China has been faced with severe haze pollution, which is hazardous to human health. Among the air pollutants, PM2.5 (particles with an aerodynamic diameter ≤ 2.5 μm) is the most dangerous because of its toxicity and impact on human health and ecosystems. However, there has been limited research on PM2.5 particle toxicity. In the present study, we collected daily PM2.5 samples from January 1 to March 31, 2018 and selected samples to extract water-soluble species, including SO42−, NO3−, WSOC, and NH4+. These samples represented clean, good, slight, moderate, and heavy pollution days. After extraction using an ultrasonic method, PM2.5 solutions were obtained. We used Chlorella as the test algae and studied the content of chlorophyll a, as well as the variation in fluorescence when they were placed into the PM2.5 extraction solution, and their submicroscopic structure was analyzed using transmission electron microscopy (TEM). The results showed that when the air quality was relatively clean and good (PM2.5 concentration ≤ 75 μg m−3), the PM2.5 extraction solutions had no inhibiting effects on Chlorella, whereas when the air quality was polluted (PM2.5 concentration > 75 μg m−3) and heavily polluted (PM2.5 concentration > 150 μg m−3), with increasing PM2.5 concentrations and exposure time, the chlorophyll a content in Chlorella decreased. Moreover, the maximum photochemical quantum yield (Fv/Fm) of Chlorella obviously decreased, indicating chlorophyll inhibition during polluted days with increasing PM2.5 concentrations. The effects on the chlorophyll fluorescence parameters were also obvious, leading to an increase of energy dissipated per unit reaction center (DIo/RC), suggesting that Chlorella could survive when exposed to PM2.5 solutions, whereas the physiological activities were significantly inhibited. The TEM analysis showed that there were few effects on Chlorella cell microstructure during clean days, whereas plasmolysis occurred during light- and medium-polluted days. With increasing pollution levels, plasmolysis became more and more apparent, until the organelles inside the cells were thoroughly destroyed and most of the parts could not be recognized.

Hormonal growth promoters (HGPs), widely used in beef cattle production globally, make their way into the environment as agricultural effluent—with potential impacts on aquatic ecosystems. One HPG of particular concern is 17β-trenbolone, which is persistent in freshwater habitats and can affect the development, morphology and reproductive behaviors of aquatic organisms. Despite this, few studies have investigated impacts of 17β-trenbolone on non-reproductive behaviors linked to growth and survival, like boldness and predator avoidance. None consider the interaction between 17β-trenbolone and other environmental stressors, such as temperature, although environmental challenges confronting animals in the wild seldom, if ever, occur in isolation. Accordingly, this study aimed to test the interactive effects of trenbolone and temperature on organismal behavior. To do this, eastern mosquitofish (Gambusia holbrooki) were subjected to an environmentally-relevant concentration of 17β-trenbolone (average measured concentration 3.0 ± 0.2 ng/L) or freshwater (i.e. control) for 21 days under one of two temperatures (20 and 30 °C), after which the predator escape, boldness and exploration behavior of fish were tested. Predator escape behavior was assayed by subjecting fish to a simulated predator strike, while boldness and exploration were assessed in a separate maze experiment. We found that trenbolone exposure increased boldness behavior. Interestingly, some behavioral effects of trenbolone depended on temperature, sex, or both. Specifically, significant effects of trenbolone on male predator escape behavior were only noted at 30 °C, with males becoming less reactive to the simulated threat. Further, in the maze experiment, trenbolone-exposed fish explored the maze faster than control fish, but only at 20 °C. We conclude that field detected concentrations of 17β-trenbolone can impact ecologically important behaviors of fish, and such effects can be temperature dependent. Such findings underscore the importance of considering the potentially interactive effects of other environmental stressors when investigating behavioral effects of environmental contaminants.

Energy production in the Williston Basin, located in the Prairie Pothole Region of central North America, has increased rapidly over the last several decades. Advances in recycling and disposal practices of saline wastewaters (brines) co-produced during energy production have reduced ecological risks, but spills still occur often and legacy practices of releasing brines into the environment caused persistent salinization in many areas. Aside from sodium and chloride, these brines contain elevated concentrations of metals and metalloids (lead, selenium, strontium, antimony and vanadium), ammonium, volatile organic compounds, hydrocarbons, and radionuclides. Amphibians are especially sensitive to chloride and some metals, increasing potential effects in wetlands contaminated by brines. We collected bed sediment and larval amphibians (Ambystoma mavortium, Lithobates pipiens and Pseudacris maculata) from wetlands in Montana and North Dakota representing a range of brine contamination history and severity to determine if contamination was associated with metal concentrations in sediments and if metal accumulation in tissues varied by species. In wetland sediments, brine contamination was positively associated with the concentrations of sodium and strontium, both known to occur in oil and gas wastewater, but negatively correlated with mercury. In amphibian tissues, selenium and vanadium were associated with brine contamination. Metal tissue concentrations were higher in tadpoles that graze compared to predatory salamanders; this suggests frequent contact with the sediments could lead to greater ingestion of metal-laden materials. Although many of these metals may not be directly linked with energy development, the potential additive or synergistic effects of exposure along with elevated chloride from brines could have important consequences for aquatic organisms. To effectively manage amphibian populations in wetlands contaminated by saline wastewaters we need a better understanding of how life history traits, species-specific susceptibilities and the physical-chemical properties of metals co-occurring in wetland sediments interact with other stressors like chloride and wetland drying.

In this work, we report on the synthesis and characterization of nanoporous bimetallic metal-organic frameworks (FeCo-BDC). Effects of synthesis time and temperature on the structures, morphology, and catalytic performance of FeCo-BDC were investigated. Scanning Electron Microscopy (SEM), Transmission electron microscopy (TEM) and Brunauer-Emmett-Teller (BET) were used to reveal the morphological and textural characteristics. The crystal structure and chemical composition of FeCo-BDC were determined by means of X-ray powder diffraction (XRD), Fourier Transform Infrared Spectroscopy (FT-IR), X-ray Photoelectron Spectroscopy (XPS), and Inductively Coupled Plasma Mass Spectrometry (ICP-MS) measurements. Interestingly, FeCo-BDC grew into the same crystal structure with different morphology in the temperature of 110–150 °C with 12–48 h. The heterogeneous catalytic activity of FeCo-BDC was tested to activate peroxydisulfate (PDS) and peroxymonosulfate (PMS) for removal of methylene blue (MB). The results found that FeCo-BDC synthesized at 150 °C with 24 h exhibited the best catalytic performance for PMS and obtained 100% of MB removal within 15 min. The abundant unsaturated metal active sites of Fe(II) and Co(II) in the skeleton of FeCo-BDC made a great contribution to the generation of sulfate (▪) and hydroxyl radicals (OH), which resulted in the excellent performance for MB degradation.

Whereas early life stages are usually considered as particularly sensitive to both organic and inorganic contaminants, field studies assessing contaminant bioaccumulation in these stages are scarce. Selenium (Se) is thought to counteract Hg toxic effects when it is found at Se:Hg molar ratios above 1. However, the variation of this ratio in key fish tissues of different early life stages is mostly unknown. The present study therefore aimed to assess Hg and Se content in gravid female tissues (gonads, muscle, liver, gut, and brain) and different life stages (egg masses, newly hatched larvae (NHL), larvae and juvenile) of Yellow Perch (YP) in a large fluvial lake (Lake Saint-Pierre, Québec, Canada). Se:Hg molar ratios were measured for each compartment in order to fill associated knowledge gaps. Total Hg (THg) and methylmercury (MeHg) concentration varied between tissue according to the following trend: Muscle > Liver > Gut > Brain > Gonads. During YP early life stages, MeHg values increased according to an ontogenetic pattern (mg/kg dw) (mean ± SEM): Egg masses (0.01 ± 0.002) < NHL (0.015 ± 0.001) < Larvae (0.14 ± 0.01) < Juveniles (0.18 ± 0.01). Se concentrations in different YP tissues showed the following trend (mg/kg dw) (mean ± SEM): Gut (3.6 ± 0.1) > Liver (2.5 ± 0.1) > Gonads (1.92 ± 0.06) > Brain (1.26 ± 0.03) > Muscle (1.23 ± 0.06). In YP early life stages, Se concentrations were highest in NHL (3.0 ± 0.2), and then decreased as follows: Egg masses (2.8 ± 0.1) > Larvae (1.37 ± 0.04) > Juveniles (0.93 ± 0.05). Se:Hg molar ratios varied considerably and were systematically above 1. This is the first study to simultaneously report Hg and Se bioaccumulation through fish life cycle.

Cerium (Ce, CeCl₃) and Erbium (Er, ErCl₃) are increasingly used in many electronic devices facilitating the alteration of their biogeochemical cycles (e.g. e-waste). Previous surveys stated that their environmental concentrations due to natural or anthropogenic events can reach up to 161 μg/L in ore mine effluent for Ce with a mean water concentration of 0.79 μg/L, and 11.9 μg/L for Er in ore mine effluents with a mean water concentration of 0.004 μg/L. Their potential effects onto aquatic organisms are still relatively unexplored. In this study, long-term multigenerational effects on Daphnia magna were assessed using various exposure times (3, 7, 14, and 21 days) in three generations (F0, F1 and F2). Each generation was exposed to environmental concentrations of Ce and Er (0.54 and 0.43 μg/L, respectively – mean values) and effects included organisms' size, parental reproduction, and survival, determination of reactive oxygen species (ROS), enzymatic activity (superoxide dismutase (SOD), catalase (CAT), and glutathione S-transferase (GST)), gene expression of ATP-binding cassette (ABC) transporter, and uptake.Results evidenced that chronic multi-generational exposure of daphnids to Ce and Er reduced survival, growth and reproduction, decreasing ROS, SOD and CAT from F0 to F2. Ce reduced the number of generated offsprings after each generation, while Er delayed the time of offsprings emergence, but not their number. ROS, SOD, CAT and GST evidenced that Er is slightly more toxic than Ce. Up- and downregulation of genes was limited, but Ce and Er activated the ABC transporters. Uptake of Ce and Er decreased through exposure time and generations.

In recent decades, rapid development of industrialization and urbanization caused adverse impact on the aqueous ecology and environment of the Huaihe River basin, China. In this work, three ²¹⁰Pb-dated sediment cores extracted from the middle reach of Huaihe River in Anhui Province, China were analyzed to elucidate the temporal trends and sources of polybrominated diphenyl ethers (PBDEs). Source diagnostics indicated that commercial Deca-BDE, Penta-BDE and Octa-BDE products and debromination of higher brominated BDE compounds were likely the PBDE sources in the Huaihe River. The prevalence of BDE-47 in the sediment cores was attributed to the extensive use of commercial Bromkal 70-5DE and Bromkal DE-71 in the region. BDE-28 was another congener that was prevalent in all sediment samples, suggesting that reductive debromination occurred in the sediments. Dramatic increase of PBDE concentrations in both three cores since the post-1980s could be attributed to the rapid expansion of production of electronic and telecommunication equipment and household usage in China. PBDE temporal trends in core S1 located at rural area mainly reflected the regional and national inputs deriving from long distance atmospheric transport, and the positive correlations between PBDE concentration in core S1 and gross domestic product (GDP) and household appliances production volumes (HPVs) were observed. PBDE inputs at site S3 mainly include the transport of contaminated water and re-suspended fine sediment particles from the upstream site S2, which was located in the industrial area and adjacent to e-waste recycling area. The government efforts to protect the environment and improve the e-waste management resulted in the progressive decrease trends in PBDE concentrations in cores S2 and S3.

PM2.5 is becoming a worldwide environmental problem, which profoundly endangers public health, thus progressively capturing public attention this decade. As a fragile target of PM2.5, the underlying mechanisms of endothelial cell damage are still obscure. According to the previous microarray data and signaling pathway analysis, a new form of cell death termed ferroptosis in the current study is proposed following PM2.5 exposure. In order to verify the vital role of ferroptosis in PM2.5-induced endothelial lesion and further understand the potential mechanism involved, intracellular iron content, ROS release and lipid peroxidation, as well as biomarkers of ferroptosis were detected, respectively. As a result, uptake of particles increases cellular iron content and ROS production. Meanwhile, GSH depletion, and the decrease of GSH-Px and NADPH play significant roles in PM2.5-induced endothelial cell ferroptosis. Moreover, significantly changed expression of TFRC, FTL and FTH1 hinted that dysfunction of iron uptake and storage is a major inducer of ferroptosis. Importantly, index monitored above can be partially rescued by lipid peroxidation inhibitor ferrostatin-1 and iron chelator deferoxamine mesylate, which mediated antiferroptosis activity mainly depends on the restoration of antioxidant activity and iron metabolism. In conclusion, our data basically show that PM2.5 enhances ferroptosis sensitivity with increased ferroptotic events in endothelial cells, in which iron overload, lipid peroxidation and redox imbalance act pivotal roles.

Microplastics pose a great threat to entire marine ecosystems, but little is known about their impacts on phytoplankton, especially for the harmful dinoflagellates. In this study, effects of micro polyvinyl chloride (mPVC) on the growth, chlorophyll content and photosynthetic efficiency of the dinoflagellate Karenia mikimotoi at different periods (0, 24, 48, 72 and 96 h) were assessed using gradient concentrations (0, 5, 25, 50 and 100 mg L⁻¹) of mPVC with a size of 1 μm. PVC microplastics had dose-dependent adverse effects on K. mikimotoi growth, chlorophyll content and photosynthetic efficiency. The density of algal cell decreased with increasing mPVC concentrations and the highest inhibitory rate (IR) was 45.8% at 24 h under 100 mg L⁻¹ of mPVC. The total chlorophyll content and chlorophyll content in a single algal cell decreased at 96 h and the ФPSⅡ and Fv/Fm decreased 25.3% and 17.1%, respectively. The SEM images provided an intuitive visual method to observe the behaviors and interactions between microplastics and microalgae. It was found from the SEM images that microalgae was wrapped by microplastic beads. The physical blockage and aggregation were also responsible for the cytotoxicity of K. mikimotoi. Our study clarified that PVC microplastics can reduce algal growth, chlorophyll content and photosynthetic efficiency, and it is beneficial to evaluate the possible impact of plastics on aquatic ecosystems.

Norfloxacin (NOR) is a persistent organic pollutant and can be effectively removed from effluent by adsorption of biochar. However, the presence of other emerging contaminants, such as surfactants, will potentially alter adsorption performance of norfloxacin by biochar and the molecular-scale mechanisms of the interaction between surfactants and biochar remain poorly understood. In this study, adsorption of norfloxacin on magnetic biochar prepared with iron-containing furfural residue (FRMB) in the presence or absence of anionic surfactants was investigated. The adsorption of NOR was significantly affected by the initial pH and anionic surfactants-sodium dodecyl sulfate (SDS) and sodium dodecyl benzene sulfonate (SDBS). In the presence of SDS and SDBS, the maximum sorption capacities of NOR were 2.33 and 1.97 times higher than that in the absence of surfactants, reached to 698.6 mg g⁻¹ and 589.9 mg g⁻¹, respectively. The optimal pH condition which was 4 indicated that electrostatic adsorption played a decisive role in the adsorption process after introduction of SDS/SDBS. The adsorption data were fitted well by the Elovich model and Freundlich model at the optimal conditions in which both SDS and SDBS were hemimicelle (0.8 mM SDS or 0.4 mM SDBS), indicating surface heterogeneity of FRMB and the adsorption mechanism was related to the assembly of surfactants on biochar. FTIR results showed that FRMB and SDS/SDBS interacted through hydrophobic action, and more complex or aggregates were formed between the NOR and biochar/SDS/SDBS. This work highlights the synergistic enhancement effects of tested surfactants on the removal of NOR by magnetic biochar from aqueous systems.