The objectives of this investigation in the HolyCross Mountain region of Poland were (1) to determineconcentration ranges and (2) mobility of elements within fivedetailed soil profiles and their apparent impact on thechemistry of some plant bioindicators, i.e. lichen thalli ofthe species Hypogymnia physodes (L.) Nyl. and Scotspine (Pinus sylvestris L.) bark. The results ofelemental determinations performed on soils and vegetationfrom the Holy Cross Mts show that some concentrations ofelements are elevated primarily due to air pollution.However, since 1994 a steady decrease in the content of S andsome heavy metals has been recorded in all the mediaexamined. Concentrations of many elements in H.physodes thalli and P. sylvestris needles of the Holy Cross Mountains are generally similarto those in other areas of Europe.

Six seaweed species of the Rhodophyta family were sampled for atwo-year period from June 1996 to August 1998 along the Atlanticcoast of Ghana (part of the Atlantic Ocean). The species wereanalysed to determine the levels of eleven chemicalelements namely: Al, As, Br, Cd, Fe, La, Mn, Ni, Hg, V and Zn byInstrumental Neutron Activation Analysis (INAA). Al, Br, Ni, andZn were measured in all the seaweed species studied. The elementAl generally showed the highest concentrations in most of thespecies analysed while Hg levels were the lowest. Precision andaccuracy of the method have been evaluated and the detectionlimits also calculated. The results showed high variability inand between species, among sampling sites and times ofcollection. The high values of metal concentrations in themacroalgae suggest that these marine organisms can be used asbiological indicators for studing marine pollution.

Bacterial contamination of drinking water wells is a commonproblem in many rural areas. Some of this contamination may berelated to manure spreading or housing of livestock; another source is on-site septic systems. Current indicator organisms are able to detect the presence of fecal contamination, but where there may be more than one potential source of fecal material, the current indicators are unable to ascertain the origin. This laboratory investigation was undertaken to determine the longevity and reliability of a selected suite of indicator organisms. Total coliform, fecal coliform, fecal streptococci and Clostridium perfringens were monitoredin a simulated contaminated groundwater environment for 6 months. All four indicator organisms were present at the end of6 months. The number of fecal streptococi bacteria decreased most noticeably, allowing assessment of relative age of contamination. C. perfringens was found to be a reliableindicator of contamination from animal manure. Fecal material from 28 different animals and three septic systems were assessed for the presence of the indicator organisms. Totalcoliform, fecal coliform and fecal streptococci were present in the fecal material of all animals tested including reptiles.C. perfringens was detected in feces from all but two of the animals assessed. Using an assemblage of indicator organisms provides more information regarding source and timingof contamination than just testing for total coliform and fecalcoliform bacteria.

Our field survey showed that thequality of shallow groundwater around the KatsuraRiver in the Kyoto Basin was strongly affected by theinfiltration of river water. Furthermore, that thedeterioration of the groundwater in the southern areato the west of the Katsura River may be related to theincrease in groundwater extraction. To clarify themechanism of groundwater deterioration, we havedeveloped a stochastic method to simulate groundwaterflow. The results showed that there was a largereduction in the groundwater level where groundwaterextraction was intense and recharge flowed from theKatsura River to the high extraction areas in thesouthern region. Another simulation showed that if thegroundwater extraction was 10% of the present removalrate, there would be little recharge from the KatsuraRiver into the groundwater and the quality of thegroundwater would be improved. Thus, we conclude thatthe cause of groundwater deterioration is probably dueto the induced recharge of deteriorated river waterfrom the Katsura River.

The difference in effect on acidification and species of aluminum speciation between HNO₃ and H₂SO₄for two contrasting types of soils in surface charge was investigated. The results show that the effect of H₂SO₄ on acidification of variable charge soils (Ferric Acrisol and Haplic Acrisol) of subtropical regions wasweaker than that of HNO₃, due to the specific adsorption of SO₄ ²⁻ and the accompanied release of OH⁻. For two constant charge soils, Haplic Luvisol andEutric Cambisol, the difference in effect between the two acids is small. The concentrations of total inorganic monomeric aluminum, Al³⁺, Al-F complexes and Al-SO₄complexes in the extract from variable charge soils are alsolower in H₂SO₄ systems than those in HNO₃ systems, whereas the reverse is true for constant charge soils, except the concentration of Al³⁺. For variable charge soils, Al-F complexes are the major species of inorganic monomeric aluminum at high pH butAl³⁺ might contribute to a largepart at low pH, while for constant charge soils Al-F complexes contribute almost exclusively to the inorganic monomeric aluminum. The presence of a large amount of SO₄ ²⁻ in the extract from the constant charge soilsleads to a higher proportion of Al-SO₄ complexes in totalinorganic monomeric aluminum than that from variable charge soil, although the numerical value of proportion is small.

The water quality of a stream affected by miningactivities was investigated on the basis of a mineralogical studyfor the related solids, and their subsequent changes weremonitored for a year, so as to clarify the impact of the acidmine drainage (AMD) to the stream. The mine-affected stream wasclassified into Ca–Mg and sulfate type, and the concentrations ofits major constituents ranged from tens to hundreds times higherthan those of the background stream. This was most likely due toacid-generating reactions involving the oxidation of sulfides inthe mineralized zone, and subsequent neutralizations involvingcalcite and chlorite as possible sources of Ca and Mg,respectively. This interpretation is consistent with thethermodynamic and mass-balance calculations. The concentrationsof the dissolved constituents changed seasonally, dependinglargely on rainfall in the mine-affected stream. However, thedramatic decrease in the ratio of Mg/Ca, independent of rainfall,indicates that some changes did occur in sources, including theheterogeneous distribution of main source materials, the changein chemical conditions, especially in pH, pe(Eh), and PCO ₂,in the reacting fluid, and the consequential solubility changesin sources. In spite of the limitations of short-term monitoring,it does provide some meaningful information in order to constructa long-term monitoring program.

Corrosion products have been takenfrom 130 copper or bronze outdoor objects all overEurope. Their chemical composition and crystalsymmetry have been determined by means of scanning electron microscopy (SEM/EDS) and X-ray powderdiffraction. Data on location, sampling, objectcharacteristics, general environment and air pollutionlevel; type, colour and chemical composition of thecorrosion layers have been obtained and evaluated by multivariate statistical analysis. The resultsverify that the highest air pollution levels are usually associated with the occurrence of thick,black or dark grey corrosion layers on copper orbronze objects, preferentially containing soot, ironoxide hydroxides, and antlerite,Cu₃(SO₄)(OH)₄. Pale green corrosionusually contains brochantite,Cu₄(SO₄)(OH)₆, and is rather associatedwith less polluted areas. Atacamite, a copper hydroxide chloride with the chemical formula Cu₂Cl(OH)₃, is preferentially observed in coastal regions.In addition, sulphur isotope analyses have beenperformed on eleven corrosion samples from citycenters. The δ³⁴S values are typically inthe region from +4 to +6‰ relative to the sulphurisotope standard CDT (Canyon Diablo Troilite) with amean value of 4.7±1.2 (1σ), therebyindicating that the sulphur in the corrosion layers,in the form of brochantite or antlerite, mainlyoriginates from a similar source despite geographicvariation, most likely sulphur contained in air pollutants.

Mercury (Hg) methylation was studied in water,sediment and Eichhornia crassipesroots of a freshwater lake, in Rio de Janeiro(Brazil). Samples were incubated with²⁰³HgCl₂ and the Me²⁰³Hg producedwas measured by liquid scintillation.Methylmercury (MeHg) production was <10⁻³% in water, low in sediment (up to5.8%) and high in E. crassipesroots (21–27%). Higher MeHg formation wasfound in aerobic conditions for the roots and inanaerobic conditions for the sediment.Methylation increased with incubation time, upto 5 days. A 3-day incubation period was used inthe majority of the assays, to avoid large scalephysico-chemical changes inside the incubationflasks. Methylation was not detected inheat-sterilized root samples. Sodium sulphatestimulated Hg methylation while sodium molybdateinhibited the process in samples incubated for3, 6, 12, 24, 48 and 72 hr. This suggeststhat sulphidogenic bacteria are responsible forthe methylation process. Experiments with rootsstored at 5 and 25 °C fordifferent periods before incubation, indicatethat methylation is modified by storage time and temperature.

The aim of this work was to evaluate antimony (Sb) as apotentially valuable tracer of the anthropogenic impact on soilsand estuarine sediments. Antimony was determined in fifteenroadsoils from a medium-size city, La Coruña (NW of Spain);and twelve sediments from two estuaries (La Coruña and Ares-Betanzos). Sb determination was accomplished in a rapid andreliable way by directly measuring the solid samples with anautomatic ultrasonic slurry sampling-ETAAS device. Soil contentsranged from 0.29 to 8.81 μg g⁻¹. A relation between Sbconcentration and motor vehicle intensity was observed.Regarding estuarine sediments, Sb amounted from 0.22 to 1.51 μg g⁻¹, for the La Coruña estuary and 0.24 to 0.71 μg g⁻¹, forthe Ares-Betanzos estuary; higher Sb values were found forcoastal locations whereas lower contents corresponded to `inner'samples. In order to confirm these findings, other pollutantswere studied: As, Cd, Co, Cr, Cu, Ni, Pb, Zn, aliphatichydrocarbons (AH) and polycyclic aromatic hydrocarbons (PAH).These values, along with the Sb ones, were subjected tomultivariate studies intended to elucidate whether Sb correlated(and to what extent) to other well-known anthropogenic pollutants. Thus, Sb became associated to Cd, Cu, Pb and Zn in road soils and to As, Cu, Zn, PAH, Pb and unresolved hydrocarbons, in sediments.