We measured the temporal and spatial trajectory of oiling from the April, 2010, Deepwater Horizon oil spill in water from Louisiana's continental shelf, the estuarine waters of Barataria Bay, and in coastal marsh sediments. The concentrations of 28 target alkanes and 43 target polycyclic aromatic hydrocarbons were determined in water samples collected on 10 offshore cruises, in 19 water samples collected monthly one km offshore at 13 inshore stations in 2010 and 2013, and in 16–60 surficial marsh sediment samples collected on each of 26 trips. The concentration of total aromatics in offshore waters peaked in late summer, 2010, at 100 times above the May, 2010 values, which were already slightly contaminated. There were no differences in surface or bottom water samples. The concentration of total aromatics declined at a rate of 73% y−1 to 1/1000th of the May 2010 values by summer 2016. The concentrations inside the estuary were proportional to those one km offshore, but were 10–30% lower. The oil concentrations in sediments were initially different at 1 and 10 m distance into the marsh, but became equal after 2 years. Thus, the distinction between oiled and unoiled sites became blurred, if not non-existent then, and oiling had spread over an area wider than was visible initially. The concentrations of oil in sediments were 100–1000 times above the May 2010 values, and dropped to 10 times higher after 8 years, thereafter, demonstrating a long-term contamination by oil or oil residues that will remain for decades. The chemical signature of the oil residues offshore compared to in the marsh reflects the more aerobic offshore conditions and water-soluble tendencies of the dissolved components, whereas the anaerobic marsh sediments will retain the heavier molecular components for a long time, and have a consequential effect on the ecosystems.

Soluble microbial products (SMPs) discharged into rivers from sewage treatment plants may increase the health risk for downstream drinking water by acting as a precursor of DBPs. Biotransformation or biodegradation could alter the characteristics of SMPs and affect the subsequent formation of DBPs. This study observed the relative contribution of chemical fractions in SMPs and explored the biodegradation of each fraction and their effect on disinfection by-products (DBPs) formation in surface water. The hydrophilic acid (HPIA) and hydrophobic acid (HPOA) constituted the major portion of the SMPs, which were dominated by fulvic acid and humic acids. The transphilic acid (TPIA) and hydrophobic bases (HPOB) were relatively minor but it contained a relative substantial portion of protein-like materials in SMPs. TPIA and HPOB produced insignificant amounts of DBP corresponding to 13% and 14% in the original samples, but they were collectively responsible for 50% of the DBPs yield. Much larger amounts of hydrophobic fractions were utilized than hydrophilic fractions after biodegradation. The increase in SUVA values indicating aromatic structures, except for HPOA fraction, was observed after biodegradation. The protein-like materials in both the HPOA and HPIA fractions and polycarboxylate-type humic acid in the HPIA fraction decreased but the enrichment of HPOA (MW > 100 kDa) and TPIA (MW < 1 kDa) was observed after biodegradation. The production of = C–H in HPIA fraction and the appearance of double peak at 1100 cm⁻¹ in TPIA and HPOB fractions occurred after biodegradation. In overall level, microorganisms effectively utilized DBP precursors from HPIA, HPOA and HPOB fractions but increased the DBPs precursors from the TPIA fraction. TPIA and HPOB fractions had higher DBP yield with chlorine but the DBPs yield of HPIA and HPOA changed little after biodegradation.

To better understand the toxicological activities of perfluorooctanoic acid (PFOA), we examined the effects of PFOA on apoptosis and its molecular mechanism in SMMC-7721 hepatoma cells. Cell viability was evaluated by MTT assay and apoptosis was determined by flow cytometry. Western blot and quantitative real-time PCR were used to examine the protein and gene expressions of Bax and Bcl-2. Our results showed that PFOA inhibited SMMC-7721 cell growth and induced apoptosis. PFOA treatment increased Bax expression and increased Bcl-2 expression at both gene and protein levels. Our study demonstrated that PFOA had toxic effects on SMMC-7721 cells, such as inhibiting cell proliferation and inducing apoptosis. Furthermore, we showed that PFOA-mediated induction of apoptosis involved inducing Bax and decreasing Bcl-2 expression as a molecular mechanism of its toxicological effects.

Microplastics (MPs) are an emerging contaminant and are confirmed to be ubiquitous in the environment. Adverse effects of MPs on aquatic organisms have been widely studied, whereas little research has focused on soil invertebrates. We exposed the soil springtail Folsomia candida to artificial soils contaminated with polyethylene MPs (<500 μm) for 28 d to explore the effects of MPs on avoidance, reproduction, and gut microbiota. Springtails exhibited avoidance behaviors at 0.5% and 1% MPs (w/w in dry soil), and the avoidance rate was 59% and 69%, respectively. Reproduction was inhibited when the concentration of MPs reached 0.1% and was reduced by 70.2% at the highest concentration of 1% MPs compared to control. The half-maximal effective concentration (EC₅₀) value based on reproduction for F. candida was 0.29% MPs. At concentrations of 0.5% dry weight in the soil, MPs significantly altered the microbial community and decreased bacterial diversity in the springtail gut. Specifically, the relative abundance of Wolbachia significantly decreased while the relative abundance of Bradyrhizobiaceae, Ensifer and Stenotrophomonas significantly increased. Our results demonstrated that MPs exerted a significant toxic effect on springtails and can change their gut microbial community. This can provide useful information for risk assessment of MPs in terrestrial ecosystems.

A growing concern for contamination due to pharmaceutical compounds in groundwater is expanding globally.The β-blocker propranolol is a β-adrenoceptors antagonist commonly detected in European groundwater bodies. The effect of propranolol on stygobiotic species (obligate groundwater dweller species) is compelling in the framework of environmental risk assessment (ERA) of groundwater ecosystems. In fact, in Europe, ERA procedures for pharmaceuticals in groundwater are based on data obtained with surrogate surface water species. The use of surrogates has aroused some concern in the scientific arena since the first ERA guideline for groundwater was issued. We performed an ecotoxicological and a behavioural experiment with the stygobiotic crustacean species Diacyclops belgicus (Copepopda) to estimate a realistic value of the Predicted No Effect Concentration (PNEC) of propranolol for groundwater ecosystems and we compared this value with the PNEC estimated based on EU ERA procedures. The results of this study showed that i) presently, propranolol does not pose a risk to groundwater bodies in Europe at the concentrations shown in this study and ii) the PNEC of propranolol estimated through the EU ERA procedures is very conservative and allows to adequately protect these delicate ecosystems and their dwelling fauna. The methodological approach and the results of this study represent a first contribution to the improvement of ERA of groundwater ecosystems.

Analysis of persistent halogenated organic pollutants (HOPs) in human follicular fluid is important given previous reports of their adverse effects on the reproductivity of women. In the present work, HOPs, including polybrominated diphenyl ethers (PBDEs) and polychlorinated biphenyls (PCBs), were analyzed in 127 follicular fluid samples collected from patients who were undergoing in vitro fertilization in Central China. The concentrations of ∑₇BDEs (sum of BDE-28, -47, −99, −100, −153, −154, and −183) in follicular fluid ranged from not detected (n.d.) to 110 ng/g lipid weight (lw), with an average of 50 ± 24 ng/g lw. BDE-100 was suggested to be an indicator of BDE congeners in follicular fluid, with the highest concentrations and showing a significantly high correlation (p < 0.01) with ∑₇BDEs. Penta-BDE products were the principal source of PBDEs in follicular fluid samples. The concentrations of ∑₇CBs (CB-28, -52, −101, −118, −138, −153, and −180) in follicular fluids ranged from n.d. to 250 ng/g lw, with an average of 77 ± 69 ng/g lw. CB-28 and CB-52 were considered to be indicator CB congeners, with tri-CBs and tetra-CBs dominating in follicular fluid. No significant correlation was observed between patient age and PBDE or PCB concentrations in follicular fluid, indicating that age was not the controlling factor influencing the bioaccumulation of most HOPs in this study.

Over the last decades, plastic debris has been identified and quantified in the marine environment. Coastal and riverine input have been recognized as sources of plastic debris, whereas oceanic gyres and sediments are understood to be sinks. However, we have a limited understanding of the fate of plastic debris in the nearshore environment. To investigate the movement and distribution of plastic debris in the nearshore environment, we collected samples at three distinct locations: below the high tide line, the turbulent zone created by the combination of breaking wave and backflush (defined as the boundary), and the outer nearshore. We estimated the abundance and physical characteristics (e.g. density, hardness, etc.) of macroplastic and microplastics. Four times and 15 times more macroplastics and microplastics are observed, respectively, at the boundary than in the outer nearshore waters, which suggests an accumulation driven by the physical properties of the plastic particles such as density, buoyancy and surface area. We further report that highly energetic conditions characteristic of the boundary area promote the long-term suspension and/or degradation of low density, highly buoyant or large surface area plastic debris, leading to their preferential accumulation at the boundary. Contrastingly, denser and low surface area plastic pieces were transported to the outer nearshore. These results emphasize the role of selective plastic movement at the nearshore driven by physical properties, but also by the combined effects of several hydrodynamics forces like wave action, wind or tide in the resuspension, as well as degradation and transport of plastic debris out of the nearshore environment.

If volatile organic compound (VOC)-contaminated soil exists underneath a building, vapors may migrate upwards and intrude into the interior air of the building. Most previous models used to simulate vapor intrusion (VI) were developed by assuming that the source was constant, although a few recent models, such as the Risk-Based Corrective Action (RBCA) Tool Kit (TK) model, have been developed to consider source depletion (SD). However, the RBCA TK model ignores the effects of building characteristics due to its assumption that the ground is not covered by the actual building it models, which leads to incorrect results since the presence of the building affects the SD. In this study, a SD model is developed based on the three processes of VI while considering the impact of key building parameters on SD. The proposed model (i.e., the SD model) still follows the law of mass conservation, and the sensitivity analysis shows that the soil-building pressure differential (dP) is an important building characteristic that affects SD. Taking trichloroethylene (TCE) for simulation in the case of a soil concentration below the saturation concentration, as the soil permeability decreases, the differences in the results between the SD model and RBCA TK model decrease; as the Peclet number decreases, the effect of the dP on the results of the SD model decreases. The new model only accounts for the migration of contaminants at the source of depletion; therefore, the model is more applicable for these contaminants, which are considered to have low-biodegradable characteristics. Furthermore, since the model emphasizes the impact of buildings on the source, it is applicable when there is a considerable building area above the source, such as large commercial buildings or residential communities with underground parking lots, which exist in most cities.

Birnessites are abundant naturally occurring minerals with high sorption and oxidation capacity that could therefore play an important role in antimony (Sb) migration and transformation. There are various types of birnessites in the environment. However, little is known about the similarities and differences in Sb oxidation and sorption on birnessites with different properties. In this study, the behavior of Sb oxidation and sorption on two contrasting birnessites (δ-MnO₂ and triclinic birnessite (TrBir)) were investigated via batch and kinetic experiments and various spectroscopic techniques. Our results showed that the reaction mechanisms between Sb and the two birnessites were similar. The edge sites of birnessites were responsible for Sb(III) oxidation. Mn(IV) was reduced to Mn(III) and Mn(II), bound with birnessites and released to the solution, respectively. Because of the rapid rate of electron transfer of adsorbed Sb(III) to birnessites, the only Sb species on δ-MnO₂ after the oxidation reaction was Sb(V). Sb(V) was adsorbed at the edge sites of birnessites by replacing the OH group of birnessites, forming corner-sharing complexes with birnessites. However, the Sb sorption and oxidation capacities of the two birnessites were significantly different. Poorly-crystallized δ-MnO₂ exhibited a much higher oxidation and sorption capacity than well-crystallized TrBir because the former had many more edge sites than the latter. This study reveals the general mechanism of the reaction between Sb and birnessite and indicates that birnessite with a high number of edge sites would exhibit a huge capacity in Sb oxidation and sorption.

Abandoned nonferrous metal(loid) tailings sites are anthropogenic, and represent unique and extreme ecological niches for microbial communities. Tailings contain elevated and toxic content of metal(loid)s that had negative effects on local human health and regional ecosystems. Microbial communities in these typical tailings undergoing natural attenuation are often very poorly examined. The diversity and inferred functions of bacterial communities were examined at seven nonferrous metal(loid) tailings sites in Guangxi (China), which were abandoned between 3 and 31 years ago. The acidity of the tailings sites rose over 31 years of site inactivity. Desulfurivibrio, which were always coupled with sulfur/sulfide oxidation to dissimilate the reduction of nitrate/nitrite, were specific in tailings with 3 years abandonment. However, genus beneficial to plant growth (Rhizobium), and iron/sulfur-oxidizing bacteria and metal(loid)-related genera (Acidiferrobacter and Acidithiobacillus) were specific within tailings abandoned for 23 years or more. The increased abundance of acid-generating iron/sulfur-oxidizing and metal(loid)-related bacteria and specific bacterial communities during the natural attenuation could provide new insights for understanding microbial ecosystem functioning in mine tailings. OTUs related to Sulfuriferula, Bacillus, Sulfurifustis, Gaiella, and Thiobacillus genera were the main contributors differentiating the bacterial communities between the different tailing sites. Multiple correlation analyses between bacterial communities and geochemical parameters indicated that pH, TOC, TN, As, Pb, and Cu were the main drivers influencing the bacterial community structures. PICRUSt functional exploration revealed that the main functions were related to DNA repair and recombination, important functions for bacterial adaptation to cope with the multi-contamination of tailings. Such information provides new insights to guide future metagenomic studies for the identification of key functions beyond metal-transformation/resistance. As well, our results offers novel outlooks for the management of bacterial communities during natural attenuation of multi-contaminated nonferrous metal(loid) tailings sites.