Tea plant is capable of hyper-accumulating fluoride (F) in leaves, suggesting drinking tea may cause excessive F intake in our body and threaten the health. This study investigated the changes in the structure, composition, and F content in the leaf cell wall of the tea (Camellia sinensis) under different F conditions to demonstrate the role of cell wall in F enrichment in tea plants. The cell wall was shown as the main part for F accumulation (67%–92%), with most of F distributed in the pectin fraction (56%–71%). With increasing F concentration, a significant increase (p < 0.05) was observed in the F content of cell wall and its components, the level of cell wall metal ions (i.e. Cu, Mg, Zn, Al, Ca, Ba, Mn), as well as the content of total cell wall materials, cellulose, and pectin. Meanwhile, the level of Cu, Mg, Zn, pectin, and cellulose was significantly positively correlated with the F content in the leaf cell wall. F addition was shown to increase the fluorescence intensity of LM19 and 2F4 antibody-labeled low-methylesterified homogalacturonans (HGs), while decrease LM20-labeled high-methylesterified HGs, coupled with an increase in the activity and gene expression of pectin methyl esterases (PMEs) in tea leaves. All these results suggest that F addition can increase pectin content and demethylesterification, leading to increased absorption of metal cations and chelation of F in the cell wall through the action of metal ions.

Dioxins are highly injurious environmental pollutants with proven toxicological effects on both animals and humans, but to date their effects on plants still need to be studied in detail. We identified a dioxin-inducible caleosin/peroxygenase isoform, PdPXG4, that is mostly expressed in leaves of date palm seedlings and exhibits a specific reductase activity towards the 13-hydroperoxide of C18:2 and C18:3 (HpODE and HpOTrE, respectively). After exposure to TCDD, lipid droplets (LDs) isolated from TCDD-exposed leaves were about 6.5–15.7-fold more active in metabolizing 13-HpOTrE compared with those isolated from non-exposed leaves. A characteristic spectrum of leaf dioxin-responsive oxylipins (LDROXYL) was detected in dioxin-exposed seedlings. Of particular importance, a group of these oxylipins, referred to as Class I, comprising six congeners of hydroxides fatty acids derived from C18:2 and C18:3, was exclusively found in leaves after exposure to TCDD. The TCDD-induced oxylipin pattern was confirmed in vitro using terbufos, a typical inhibitor towards the PdPXG4 peroxygenase activity. Of particular interest, the response of terbufos-pretreated protoplasts to TCDD was drastically reduced. Together, these findings suggest that PdPXG4 is implicated in the establishment of a dioxin-specific oxylipin signature in date palm leaves soon after their exposure to these pollutants.

We investigated the fates of seven neonicotinoids (NNIs) in full-scale drinking water treatment plants and assessed human exposure to NNIs through consuming drinking water. The total NNI concentrations in raw water and treated water samples from the drinking water treatment plants were 20.4–166 ng/L (median 118 ng/L) and 1.11–94.7 ng/L (median 20.4 ng/L), respectively. The dinotefuran (DIN) concentrations in raw water collected in different seasons were different, and the highest DIN concentration was found in summer. The drinking water treatment processes removed >91% of the NNIs except DIN and thiamethoxam (THIAM), for which the mean removal rates were 70% and 74%, respectively. The removal rates for all of the NNIs were higher for the granular activated carbon filtration process (mean 83.5%) than the other drinking water treatment plant processes (coagulation/sedimentation 22.3%, ozonation 29.2%). However, the removal rates in the granular activated carbon process were lower for DIN and THIAM (61.0% and 59.2%, respectively) than the other NNIs. Significant correlations were found between the NNI removal rates and physicochemical properties (solubility in water and log (octanol–water partition coefficient)). The estimated mean human exposure to NNIs in drinking water was 0.528 ng/(kg body weight d).

Health risks caused by exposure to black carbon (BC) and nanoparticles (NP) are well studied, although no standard currently exists for them worldwide. Exposure to children may lead to serious health effects due to their increased vulnerability and longer time spend inside the classrooms, making it important to assess the factors that affect air quality in preschools. Thus, this work aims to evaluate indoor-outdoor (I/O) relationships of NPs in the 10–420 nm range, BC and volatile organic compounds (VOCs) at rural and urban preschools (aged 3–5 years) between May 2016 and July 2017. Factorial analysis was applied to identify the possible emission sources. Prior communalities were estimated by the squared multiple correlations with all other variables. We used the varimax rotation method and the criterion for factor selection was the number of eigenvalues greater than one. Results indicate that BC and NP were 4- and 3.2-times higher in urban outdoor caused by traffic emissions, respectively. Highest concentrations occurred during rush hours and during the pickup time of children. In urban school, BC was directly related to accumulation mode (N₄₉₋₂₀₅), while in the rural area, BC was related to local traffic and particles from pulp industries in the regional background. Nucleation mode (N₁₁₋₃₆) was related to traffic emissions in urban school, while in the rural school was related with secondary formation of particles. Mean I/O ratios of BC and NP in the urban (0.54; 0.51) and rural (0.71; 0.91) schools, respectively, suggested that their higher concentrations occurred in outdoors. VOCs were higher indoor in urban (I/O = 1.97) and rural (I/O = 2.22) sites, indicating these pollutants are generated inside, regardless of urban or rural sites. These findings suggest the necessity of improving ventilation and commuting styles to lower the exposure of children to air pollutants in and around school environments.

Isoflavones (ISOs) are naturally occurring endocrine-disrupting compounds. Few human studies have evaluated the effects of ISO exposure on neonatal anthropometry. This study aimed to examine the associations of maternal soy product consumption and urinary ISO concentrations, including genistein, daidzein, glycitein, and equol, with neonatal anthropometry, based on a Chinese cohort study. In Shanghai-Minhang Birth Cohort Study, pregnant women at 12–16 weeks of gestation were recruited, and they completed a structured questionnaire to assess soy product consumption during pregnancy. They also provided a single spot urine sample for the ISO assay. Neonatal anthropometric indices (birth weight; arm, waist, and head circumference; and triceps, back, and abdominal skinfold thickness) were measured at birth. Multivariable linear regression analysis was performed among the 1188 mother-infant pairs to examine the associations between maternal soy product consumption and neonatal anthropometry. The same statistical model was applied to examine the associations between maternal ISO exposure and neonatal anthropometry among 480 mother-infant pairs. Neonate girls born to mothers who “sometimes” and “frequent” consumed soy products had 169.1 g (95% confidence interval [CI], −68.9–407.1) and 256.5 g (95% CI, 17.1–495.8) higher birth weight, respectively, than those born to mothers who “never” consumed soy products during pregnancy. We observed consistent associations between higher maternal urine ISO concentrations and increased anthropometric indices (birth weight, arm and waist circumference, and triceps and abdominal skinfold thickness) in neonate girls, while no association was observed among boys. The findings suggested that maternal dietary ISO intake during pregnancy is associated with fetal development in a sex-specific pattern. In addition, follow-up studies are required to evaluate whether the observed changes in anthropometric indices at birth are associated with health conditions later in life.

Pollutant emissions in ship exhaust have been continually increasing. SO₂ is one of the main gaseous pollutants in ship exhaust, resulting from the use of marine heavy fuel oil with high sulfur content. Therefore, it is necessary to detect the fuel sulfur content (FSC) to regulate ship exhaust emissions. Optical remote sensing methods, such as differential optical absorption spectroscopy (DOAS), light detection and ranging (LIDAR), and ultraviolet (UV) camera techniques, are regarded as simple and effective remote monitoring methods. One common technique is to estimate the SO₂ concentration in a ship plume using its local optical characteristics and use this to calculate FSC. One drawback of this technique is that there are always errors in the estimations of the SO₂ concentration despite the continuous improvement of such estimations. Another drawback is that calculating FSC from SO₂ often requires additional measurement methods. Here, a sulfur content prediction model based on a deep convolutional neural network using a UV camera is introduced. First, a ship benchmark test is performed. In the test, a large number of ultraviolet characteristic images of the ship exhaust plume are taken with a UV camera and the corresponding FSC data are collected. Next, a visual geometry group (VGG)-16 convolutional neural network model based on transfer learning is built. The model extracts all the features of the exhaust plume image as input data to the deep neural network and outputs the predicted FSC as a classification label. The results show that the model can predict the FSC value with high accuracy corresponding to the exhaust plume image. This study proves that it is theoretically feasible to apply a convolutional neural network to learn features of ultraviolet ship exhaust plume images for FSC predictions, which can provide guidance for the remote regulation of ship exhaust emissions.

Environmental pollution is a widespread problem, which has seriously threatened human health and led to an increase of human diseases. Therefore, it is critical to evaluate environmental pollutants quickly and efficiently. Because of obvious inter-species differences between animals and humans, and lack of physiologically-relevant microenvironment, animal models and in vitro two-dimensional (2D) models can not accurately describe toxicological effects and predicting actual in vivo responses. To make up the limitations of conventional environmental toxicology screening, organ-on-a-chip (OOC) systems are increasingly developing. OOC systems can provide a well-organized architecture with comparable to the complex microenvironment in vivo and generate realistic responses to environmental pollutants. The feasibility, adjustability and reliability of OCC systems make it possible to offer new opportunities for environmental pollutants screening, which can study their metabolism, collective response, and fate in vivo. Further progress can address the challenges to make OCC systems better investigate and evaluate environmental pollutants with high predictive power.

Humans are extensively exposed to polycyclic aromatic hydrocarbons (PAHs) daily via multiple pathways. Epidemiological studies have demonstrated that occupational exposure to PAHs increases the risk of lung cancer, but related studies in the general population are limited. Hence, we conducted a case-control study among the Chinese general population to investigate the associations between PAHs exposure and lung cancer risk and analyze the modifications of genetic polymorphisms in DNA repair genes. In this study, we enrolled 122 lung cancer cases and 244 healthy controls in Wuhan, China. Urinary PAHs metabolites were determined by gas chromatography-mass spectrometry, and rs25487 in X-ray repair cross-complementation 1 (XRCC1) gene was genotyped by the Agena Bioscience MassARRAY System. Then, multivariable logistic regression models were performed to estimate the potential associations. We found that urinary hydroxynaphthalene (OH-Nap), hydroxyphenanthrene (OH-Phe) and the sum of hydroxy PAHs (∑OH-PAHs) levels were significantly higher in lung cancer cases than those in controls. After adjusting for gender, age, BMI, smoking status, smoking pack-years, drinking status and family history, urinary ∑OH-Nap and ∑OH-Phe levels were positively associated with lung cancer risk, with dose-response relationships. Compared with those in the lowest tertiles, individuals in the highest tertiles of ∑OH-Nap and ∑OH-Phe had a 2.13-fold (95% CI: 1.10, 4.09) and 2.45-fold (95% CI: 1.23, 4.87) increased risk of lung cancer, respectively. Effects of gender, age, smoking status and smoking pack-years on the associations of PAHs exposure with lung cancer risk were shown in the subgroup analysis. Furthermore, associations of urinary ∑OH-Nap and ∑OH-PAHs levels with lung cancer risk were modified by XRCC1 rs25487 (Pᵢₙₜₑᵣₐcₜᵢₒₙ ≤ 0.025), and were more pronounced in wild-types of rs25487. These findings suggest that environmental exposure to naphthalene and phenanthrene is associated with increased lung cancer risk, and polymorphism of XRCC1 rs25487 might modify the naphthalene exposure-related lung cancer effect.

The world's oceans are under increasing pressure from anthropogenic activities, including significant and rapidly increasing inputs of plastic pollution. Seabirds have long been considered sentinels of ocean health, providing data on physical and chemical pollutants in their marine habitats. However, long-term data that can elucidate important patterns and changes in seabird exposure to marine pollutants are relatively limited but are urgently needed to identify and support effective policy measures to reduce plastic waste. Using up to 12 years of data, we examined the benefits and challenges of different approaches to monitoring plastic in seabirds, and the relationship between plastic and body size parameters. We found the mass and number of ingested plastics per bird varied by sample type, with lavage and road-kill birds containing less plastic (9.17–9.33 pieces/bird) than beach-washed or otherwise dead birds (27.62–32.22 pieces/bird). Beached birds therefore provide data for only a particular subset of the population, mostly individuals in poorer body condition, including those severely impacted by plastics. In addition, the mass and number of plastics in beached birds were more variable, therefore the sample sizes required to detect a change in plastic over time were significantly larger than for lavaged birds. The use of lavaged birds is rare in studies of plastic ingestion due to ethical and methodological implications, and we recommend future work on ingested plastics should focus on sampling this group to ensure data are more representative of a population's overall exposure to plastics.

We developed and validated a trace analytical method for the simultaneous determination of 10 benzophenone (BP)-type ultraviolet filters (BPs; BP, BP-1, BP-2, BP-3, BP-8, 2-hydroxybenzophenone [2-OHBP], 4-hydroxybenzophenone [4-OHBP], 4-methylbenzophenone [4-MBP], methyl-2-benzoylbenzoate [M2BB], and 4-benzoylbiphenyl [PBZ]) to analyze BPs in 110 commercial fish samples. The quick, easy, cheap, effective, rugged, and safe (QuEChERS) technique coupled with ultrahigh-performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) was employed. The developed method exhibited satisfactory linearity (R² > 0.993), favorable precision with intraday and interday relative standard deviation ranges of 1.0%–26.6% and 2.3%–29.3%, respectively, and a limit of detection ranging from 0.001 to 0.1 ng/g. BP and 2-OHBP were detected in 100% of the samples; BP-3, 4-OHBP, PBZ, and 4-MBP were detected in >70% of the samples; M2BB was detected in 33% of the samples, and BP-2 and BP-8 were not detected. Higher mean (minimum–maximum) levels of 2-OHBP and BP were found in fish samples with low lipid content, ranging from 18 (1.1–218.3) to 10 (0.5–45.4) ng/g, and those with high lipid content, ranging from 22 (1.5–76.4) to 9.6 (5.2–18.5) ng/g; low levels of the remaining six BPs were found, ranging from 0.01 to 0.9 ng/g. The mean 2-OHBP levels were 21.3, 14.4, and 30.2 ng/g for fish samples obtained from aquaculture in Taiwan, the wild in Taiwan, and different countries, respectively, with levels up to 218.3 ng/g (field eel) for a sample from Indonesia. BPs may not pose a health risk to Taiwanese adults through the consumption of fish; however, additional studies are needed to examine the risk that these contaminants may pose to ecosystems and human health.