Air pollution, which includes particulate matter (PM), is classified in group 1 as a carcinogen to humans by the International Agency for Research in Cancer. Specifically, PM exposure has been associated with lung cancer in patients living in highly polluted cities. The precise mechanism by which PM is linked to cancer has not been completely described, and the genotoxicity induced by PM exposure plays a relevant role in cell damage. In this review, we aimed to analyze the types of DNA damage and alterations in DNA repair pathways induced by PM exposure, from both epidemiological and toxicological studies, to comprehend the contribution of PM exposure to carcinogenesis. Scientific evidence supports that PM exposure mainly causes oxidative stress by reactive oxygen species (ROS) and the formation of DNA adducts, specifically by polycyclic aromatic hydrocarbons (PAH). PM exposure also induces double-strand breaks (DSBs) and deregulates the expression of some proteins in DNA repair pathways, precisely, base and nucleotide excision repairs and homologous repair. Furthermore, specific polymorphisms of DNA repair genes could lead to an adverse response in subjects exposed to PM. Nevertheless, information about the effects of PM on DNA repair pathways is still limited, and it has not been possible to conclude which pathways are the most affected by exposure to PM or if DNA damage is repaired properly. Therefore, deepening the study of genotoxic damage and alterations of DNA repair pathways is needed for a more precise understanding of the carcinogenic mechanism of PM.

Surface and water bodies in many parts of the world are affected due to eutrophication, contamination and depletion. The approach of wastewater treatment using algae for eliminating nutrients and other pollutants from domestic wastewater is growing interest among the researchers. However, sustainable treatment of the wastewater is considered to be important in establishing more effective nutrient and pollutant reduction using algal systems. In comparison to the conventional method of remediation, there are opportunities to commercially viable businesses interest with phycoremediation, thus by achieving cost reductions and renewable bioenergy options. Phycoremediation is an intriguing stage for treating wastewater since it provides tertiary bio-treatment while producing potentially valuable biomass that may be used for a variety of applications. Furthermore, the phycoremediation provides the ability to remove heavy metals as well as harmful organic substances, without producing secondary contamination. In this review, the role of microalgae in treating different wastewaters and the process parameters affecting the treatment and future scope of research have been discussed. Though several algae are employed for wastewater treatment, species of the genera Chlamydomonas, Chlorella, and Scenedesmus are extensively utilized. Interestingly, there is a vast scope for employing algal species with high flocculation capacity and adsorption mechanisms for the elimination of microplastics. In addition, the algal biomass generated during phycoremediation has been found to possess high protein and lipid contents, promising their exploitation in biofuel, food and animal feed industries.

Rhizospheric microorganisms such as denitrifying bacteria are able to affect ‘rhizobioaugmention’ in aquatic plants and can help boost wastewater purification by benefiting plant growth, but little is known about their effects on the production of plant root exudates, and how such exudates may affect microorganismal nitrogen removal. Here, we assess the effects of the rhizospheric Pseudomonas inoculant strain RWX31 on the root exudate profile of the duckweed Spirodela polyrrhiza, using gas chromatography/mass spectrometry. Compared to untreated plants, inoculation with RWX31 specifically induced the exudation of two sterols, stigmasterol and β-sitosterol. An authentic standard assay revealed that stigmasterol significantly promoted nitrogen removal and biofilm formation by the denitrifying bacterial strain RWX31, whereas β-sitosterol had no effect. Assays for denitrifying enzyme activity were conducted to show that stigmasterol stimulated nitrogen removal by targeting nitrite reductase in bacteria. Enhanced N removal from water by stigmasterol, and a synergistic stimulatory effect with RWX31, was observed in open duckweed cultivation systems. We suggest that this is linked to a modulation of community composition of nirS- and nirK-type denitrifying bacteria in the rhizosphere, with a higher abundance of Bosea, Rhizobium, and Brucella, and a lower abundance of Rubrivivax. Our findings provide important new insights into the interaction of duckweed with the rhizospheric bacterial strain RWX31 and their involvement in the aquatic N cycle and offer a new path toward more effective bio-formulations for the purification of N-polluted waters.

Urbanisation of riverine landscape is an increasing threat to the functionality of river ecosystems. In this study, we identify and classify macroinvertebrates indicator signature traits and ecological preferences.We hypothesised that urban pollution would differentially influence the distribution of macroinvertebrate traits and ecological preferences along a gradient of water quality deterioration. Hence, we identified and classified potential biological indicators traits and ecological preferences that were deemed tolerant of or sensitive to urban pollution gradient in the Niger Delta region of Nigeria. Physico-chemical variables (water temperature, depth, flow velocity, dissolved oxygen, biochemical oxygen demand, electrical conductivity (EC), nitrate, phosphate), and macroinvertebrates were collected from 2008 to 2012 seasonally during the wet and dry seasons once in a month in 11 stations in eight river systems. The results based on RLQ, fourth-corner and Kruskal-Wallis analyses indicate that traits/ecological preferences such as tegumental/cutaneous respiration, cased/tubed body armouring, a preference for silty water, bivoltinism, burrowing and a high tolerance for oxygen depletion, were statistically significantly associated with the heavily impacted stations. These traits were positively correlated with physico-chemical variables such as EC, nitrate and phosphate indicative of urban pollution. On the other hand, traits/ecological preferences such as permanent attachment, crawling, swimming, univoltinism and a moderate sensitivity to oxygen depletion were associated with the least impacted stations and were negatively correlated with physico-chemical variables indicative of urban pollution. Overall, the observed differential responses of traits and ecological preferences to urban pollution along a gradient of water quality impairment suggest that traits and ecological preferences can serve as useful biological indicators and thus supports the growing evidence of the utility of the trait-based approach.

In several countries, flower import regulations are restricted to food security, by establishing maximum residue limits (MRL) for pesticides in flower-based food products and biosafety, in order to limit the circulation of vectors, pests and exotic species across borders. In this context, the lack of limits on pesticides in flower-products for ornamental purposes can influence the pesticide overuse in production areas, as well as the transfer of contaminated products between countries. Therefore, the purpose of this review was to discuss possible adverse effects on human and environmental health of pesticides used in floriculture, evaluating regulations on the use of these pesticides in the main importing and flower-producing countries. This review included 92 documents. The use of 201 compounds was identified by interviews and analytical measurements. Among them, 93 are banned by the European Union (EU), although 46.3 % of these compounds have been identified in samples from European countries. Latin American countries have a large number of scientific publications on pesticides in flower production (n = 51), while the EU and China have less studies (n = 24) and the United States and Japan have no studies. Regarding adverse health effects, poorer neurobehavioral development, reproductive disorders, congenital malformations and genotoxicity have been reported for residents of flower production areas and workers throughout the flower production cycle. Studies including water samples show overuse of pesticides, while environmental impacts are related to water and air contamination, soil degradation and adverse effects on the reproduction and development of non-target organisms. This review points out that the absence of MRL for non-edible flowers can be crucial for the trade of contaminated products across borders, including pesticides banned in importing countries. Furthermore, setting limits on flowers could reduce the use of pesticides in producing countries.

Between 9 March and 18 May 2020, strict lockdown measures were adopted in Italy for containing the COVID-19 pandemic: in Rome, despite vehicular traffic on average was more than halved, it was not observed a evident decrease of the airborne particulate matter (PM) concentrations, as assessed by air quality data. In this study, daily PM₁₀ filters were collected from selected automated stations operated in Rome by the regional network of air quality monitoring: their magnetic properties – including magnetic susceptibility, hysteresis parameters and FORC (first order reversal curves) diagrams - were compared during and after the lockdown, for outlining the impact of the COVID-19 measures on airborne particulate matter. In urban traffic sites, the PM₁₀ concentrations did not significantly change after the end of the lockdown, when vehicular traffic promptly returned to its usual levels; conversely, the average volume and mass magnetic susceptibilities approximately doubled, and the linear correlation between volume magnetic susceptibility and PM₁₀ concentration became significant, pointing out the link between PM₁₀ concentrations and the increasing levels of traffic-related magnetic emissions. Magnetite-like minerals, attributed to non-exhaust brakes emissions, dominated the magnetic fraction of PM₁₀ near urban traffic sites, with natural magnetic components emerging in background sites and during exogenous dusts atmospheric events. Magnetic susceptibility constituted a fast and sensitive proxy of vehicular particulate emissions: the magnetic properties can play a relevant role in the source apportionment of PM₁₀, especially when unsignificant variations in its concentration levels may mask important changes in the traffic-related magnetic fraction. As a further hint, increasing attention should be drawn to the reduction of brake wear emissions, that are overcoming by far fuel exhausts as the main particulate pollutant in traffic contexts.

Biomass burning, a recurring global phenomenon is also considered an environmental menace, making headlines every year in India with onset of autumn months. Agriculture is demographically the broadest economic sector and plays a significant role in the overall socio-economic fabric of India. Hence, disposal of crop residue is done mainly by burning leading to deterioration of air quality. Residue burning in parts of India is blamed for changing air quality in nearby cities. The spatial distribution of these emissions has always been a challenge due to various data constraints. We hereby present a comprehensive spatially resolved seasonal high resolution gridded (∼10 km × ∼10 km) emission inventory of major pollutants from crop residue burning source in India for the latest year 2018. The winter months contributes almost around ∼50% of total emission followed by summer (∼48%), which is the prime cause of changing air quality in nearby cities. Among all the crops; rice, wheat, maize and sugarcane accounts ∼90% of total PM₁₀ load in the country. The estimated emission for PM₂.₅, PM₁₀, BC and OC, CO, NOx, SO₂, VOC, CH₄ and CO₂ are found to 990.68 Gg/yr, 1231.26 Gg/yr, 123.33 Gg/yr, 410.99 Gg/yr, 11208.18 Gg/yr, 484.55 Gg/yr, 144.66 Gg/yr, 1282.95 Gg/yr, 785.56 Gg/yr and 262051.06 Gg/yr respectively. The cropping pattern and its role in different geographic regions are analysed to identify all potential emission hotspots regions scattered across the country. The developed gridded emissions inventory is envisaged to serve as an important input to regional atmospheric chemistry transport model to better quantify its contribution in deteriorating air quality in various regions of India, paving the way to policy makers to better plan the mitigation and control strategies. The developed fundamental tool is likely to be useful for air quality management.

To understand the characteristics of particulate matter (PM) and other air pollutants in Xinjiang, a region with one of the largest sand-shifting deserts in the world and significant natural dust emissions, the concentrations of six air pollutants monitored in 16 cities were analyzed for the period January 2013–June 2019. The annual mean PM₂.₅, PM₁₀, SO₂, NO₂, CO, and O₃ concentrations ranged from 51.44 to 59.54 μg m⁻³, 128.43–155.28 μg m⁻³, 10.99–17.99 μg m⁻³, 26.27–31.71 μg m⁻³, 1.04–1.32 mg m⁻³, and 55.27–65.26 μg m⁻³, respectively. The highest PM concentrations were recorded in cities surrounding the Taklimakan Desert during the spring season and caused by higher amounts of wind-blown dust from the desert. Coarse PM (PM₁₀₋₂.₅) was predominant, particularly during the spring and summer seasons. The highest PM₂.₅/PM₁₀ ratio was recorded in most cities during the winter months, indicating the influence of anthropogenic emissions in winters. The annual mean PM₂.₅ (PM₁₀) concentrations in the study area exceeded the annual mean guidelines recommended by the World Health Organization (WHO) by a factor of ca. ∼5–6 (∼7–8). Very high ambient PM concentrations were recorded during March 19–22, 2019, that gradually influenced the air quality across four different cities, with daily mean PM₂.₅ (PM₁₀) concentrations ∼8–54 (∼26–115) times higher than the WHO guidelines for daily mean concentrations, and the daily mean coarse PM concentration reaching 4.4 mg m⁻³. Such high PM₂.₅ and PM₁₀ concentrations pose a significant risk to public health. These findings call for the formulation of various policies and action plans, including controlling the land degradation and desertification and reducing the concentrations of PM and other air pollutants in the region.

Inhalation of airborne antibiotic resistance genes (ARGs) can lead to antimicrobial resistance and potential health risk. In modern society, increasing individuals stay more indoors, however, studies regarding the exposure to airborne ARGs in indoor environments and the associated risks remain limited. Here, we compared the variance of aerosol-associated ARGs, bacterial microbiomes, and their daily intake (DI) burden in dormitory, office, and outdoor environments in a university in Tianjin. The results indicated that compared to outdoor aerosols, indoors exhibited significantly higher absolute abundance of both ARG subtypes and mobile genetic elements (MGEs) (1–7 orders of magnitude), 16S rRNA genes (2–3 orders), and total culturable bacteria (1–3 orders). Furthermore, we observed that significantly different airborne bacterial communities are the major drivers contributing to the variance of aerosol-associated ARGs in indoor and outdoor aerosols. Notably, the high abundances of total bacteria, potential pathogenic genera, and ARGs (particularly those harbored by pathogens) in indoor and outdoor aerosols, especially in indoors, may pose an increased exposure risk via inhalation. The successful isolation of human pathogens such as Elizabethkingia anopheles, Klebsiella pneumonia, and Delftia lacustris resistant to the “last-resort” antibiotics carbapenems and polymyxin B from indoor aerosols further indicated an increased exposure risk in indoors. Together, this study highlights the potential risks associated with ARGs and their inhalation to human health in indoor environments.

Overexpression of estrogen receptor β (ERβ) in endometrium contributes to endometriosis (EM) pathogenesis. Trimethylation of the H3 lysine (K) 4 (H3K4me3) in promoters is strongly correlated with gene expression. This study aimed to explore the effects of bisphenol A (BPA) exposure on EM development from the perspective of the regulation of ERβ expression in eutopic endometrium via the H3K4me3-related epigenetic pathway. A mouse EM model was established to investigate the effects of BPA. Immortalized human normal endometrial stromal cells (iESCs) were cultured and treated with BPA to explore the underlying mechanism. Eutopic endometria from patients with or without EM were collected and analyzed. Results showed that BPA elevated ERβ expression in mouse eutopic endometrium and promoted lesion growth. BPA also promoted WD repeat domain 5 (WDR5) expression and upregulated H3K4me3 levels in the ERβ promoter and Exon 1. Further research indicated that WDR5 interacted with tet methylcytosine dioxygenase 2 (TET2), while BPA exposure enhanced the interaction between these two proteins, promoted the recruitment of the WDR5/TET2 complex to the ERβ promoter and Exon 1, and inhibited DNA methylation of CpG islands. The WDR5/TET2 interaction was essential for BPA-induced ERβ overexpression. Enhanced WDR5/TET2 interaction was also observed in eutopic endometria from EM patients. Further results showed that BPA upregulated WDR5 expression through the G protein-coupled estrogen receptor (GPER)-mediated PI3K/mTOR signaling pathway. In conclusion, our study suggests that BPA exposure promotes EM development by upregulating ERβ expression in eutopic endometrium via the WDR5/TET2-mediated epigenetic pathway.