To reduce negative environmental impacts from human aquaculture activities, the red alga Gracilaria lemaneiformis was co-cultured with the fish Pseudosciaena crocea in an integrated multi-trophic aquaculture (IMTA) system for 35d in Yantian Bay. The eutrophication index value decreased from 14.5 to 8.4 after seaweeds were co-cultured in cage farming areas, which indicated that the eutrophic water column in Yantian Bay could be mediated by IMTA. Total DIN and DIP of the tidal input and output were 9.23kg, 0.19kg and 11.08kg, and 0.27kg, respectively. Total 5.24kg of dissolved N and 0.81kg of dissolved P were released from IMTA system. These results indicate that G. lemaneiformis co-cultured in IMTA system could not completely remove all excess nutrients. In theory, at least 324.48kg of seaweed seedlings would be required to balance excess nutrients generated from fish cages.

The paper analyses the building process of a GHG emissions inventory, which would likely enable more strongly cities to manage their efforts and set realistic targets for emission reduction. The work includes the determination of GHG emission estimation tools and approaches used for estimation of key sources of these emissions at the local level. The actual task is the determination of major GHG emitting sectors including the key characteristics of these emissions sources at the local level, establishing targets for emissions reductions at Wroclaw urban area, Poland. The sectoral GHG emission trends in the selected urban area including a comparison of carbon dioxide (CO2) emission level with national inventory data are presented. The total GHG emissions from Wroclaw municipality in 2013 were 7.2 percent lower than those in 1990. Energy consumption is a major contributor of emissions responsible for 63% of citywide CO2 through the consumption of electricity and district and individual heating using coal and natural gas energy sources. The proposed data structure is connected in an informative Sankey diagram for all anthropogenic GHG emissions occurred in Wroclaw municipality in 2013. In order to determine the City progress towards urban carbon neutrality, reducing emissions 25% by 2020 and 80% by 2050, as well as the future risks and impacts of GHG emissions on climate change the ‘business as usual’ final energy consumption and emission scenarios are provided.

Aggressive behavior is crucial for maintaining social hierarchy in anemonefish. Endocrine disrupting chemicals such as EE2 may affect fish social hierarchy via disrupting their aggression. In this study, we aimed to characterize the effects of 17α-ethinylestradiol (EE2) on aggressive behavior and social hierarchy in the false clown anemonefish (Amphiprion ocellaris). In the laboratory experiment, juvenile anemonefish were randomly distributed to separated tanks to form small colonies of three individuals and were fed with EE2-dosed diet (100ng/g food) or a control diet for 90d. Through the experiment, each tank was videotaped and behavioral indicators of social status, including aggressive behavior, submissive response, and shelter utilization, were quantitatively analyzed from the videos. The EE2 exposure caused a higher frequency of intra-colonial aggressive interactions and a less stable social hierarchy. Our findings demonstrate the importance of examining the effects of endocrine disrupting chemicals on the social behavior of coral reef fish.

Since Lagrangian model coefficients vary with different conditions, it is necessary to calibrate the model to obtain optimal coefficient combination for special oil spill accident. This paper focuses on proposing a new method to calibrate Lagrangian model with time series of Envisat ASAR images. Oil slicks extracted from time series images form a detected trajectory of special oil slick. Lagrangian model is calibrated by minimizing the difference between simulated trajectory and detected trajectory. mean center position distance difference (MCPD) and rotation difference (RD) of Oil slicks' or particles' standard deviational ellipses (SDEs) are calculated as two evaluations. The two parameters are taken to evaluate the performance of Lagrangian transport model with different coefficient combinations. This method is applied to Penglai 19-3 oil spill accident. The simulation result with calibrated model agrees well with related satellite observations. It is suggested the new method is effective to calibrate Lagrangian model.

Coolia are marine benthic dinoflagellates which are globally distributed and potentially toxic. This study provides the first investigation of species diversity and toxicity assessment of Coolia in Hong Kong waters. Fifty-one strains of four Coolia species, including C. malayensis, C. canariensis, C. tropicalis, and C. palmyrensis, were isolated from twelve sub-tidal habitats, and identified phylogenetically using 28S rDNA sequences. Exposure experiments (48-hour) demonstrated that the algal lysates extracted from the four Coolia species exhibited different toxic effects on the lethality and abnormality of two invertebrate larvae, i.e., brine shrimp Artemia franciscana and sea urchin Heliocidaris crassispina. Heliocidaris crassispina was more sensitive to the toxic effects of Coolia species than A. franciscana. Toxicity tests from both larvae revealed that C. malayensis was generally more toxic, and caused higher mortality rates when compared with the other three species. The emerging threat of harmful benthic dinoflagellates to marine environments and sensitive biota is discussed.

Effective emulsification plays an important role in the treatment of marine oil spills. The negative effects of chemical surfactants have necessitated a search for alternative dispersant that are sustainable and environmentally-friendly. To identify alternate dispersants, oil-in-seawater emulsions stabilized by hydrocarbon-degrading bacteria were investigated. After individual immobilization and surface-modification, the hydrocarbon-degrading bacteria, Bacillus cereus S-1, was found to produce a stable oil-in-seawater Pickering emulsion, which was similar to particle emulsifiers. The individual immobilization and surface-modification process improved the surface hydrophobicity and wettability of the bacterial cells, which was responsible for their effective adsorption at the oil–water interface. Through effective emulsification, the biodegradation of oil was remarkably facilitated by these treated bacteria, because of the increased interfacial area. By combining the emulsification and biodegradation, the results of this reported work demonstrated a novel approach for developing environmentally-friendly bioremediation technology in the field of oil treatment.

A review of the state of the art in oil spill modeling, focused on the period from 2000 to present is provided. The review begins with an overview of the current structure of spill models and some lessons learned from model development and application and then provides guiding principles that govern the development of the current generation of spill models. A review of the basic structure of spill models, and new developments in specific transport and fate processes; including surface and subsurface transport, spreading, evaporation, dissolution, entrainment and oil droplet size distributions, emulsification, degradation, and sediment oil interaction are presented. The paper concludes with thoughts on future directions in the field with a primary focus on advancements in handling interactions between Lagrangian elements.

This work carries out a landscape analysis for the last 60years to compare the degree of preservation of two areas on the same Italian coastline characterized by different environmental protection levels: a National designated protected areas and a highly tourist coastal destination. The conversion of natural land-covers into human land uses were detected for protected and unprotected coastal stretches highlighting that the only establishment of a protected area is not enough to stem undesirable land-use outcomes. A survey analysis was also conducted to assess attitudes of beach users and to evaluate their perception of natural habitats, beach and coastal water quality, and coastal dynamic over time. The results of 2071 questionnaires showed that there is similarity between subjective and objective data. However, several beach users perceived a bad quality of coastal water in the legally unprotected coastal area. The implications from a planning and management perspective are discussed.

Effects of water-based drill cuttings on sediment reworking activity were studied on two important benthic bioturbators (the bivalve Abra segmentum and the brittle star Amphiura filiformis) using thin aquaria, fluorescent-dyed sediment particles (luminophores), time lapse photography and image analysis. In the present context, sediment reworking activity was measured as maximum mixing depth and total amount of luminophores transported below the sediment-water interface. There was a significant reduction in the amount of downward transported luminophores in drill cuttings treatments compared to controls with added natural sediments for both species, which also was true regarding maximum mixing depth for A. segmentum. Further, A. filiformis showed a clearly delayed burrowing of luminophores in the drill cuttings treatment compared to control. To conclude, the study showed that water-based drill cuttings have the potential to reduce sediment reworking. Further, it is evidenced that water-based drill cuttings not only cause burial effects.

Fish larvae of four SE Brazilian estuaries were investigated to assess if the larval fish assemblages reflect the ecological status of estuaries. All samples were collected in the same water mass to guarantee similar natural water parameters, assuring that major differences among estuaries were related to anthropogenic pressures. Water temperature, oxygen, pH, chlorophyll a, faecal coliforms, nutrient load and total particulate matter were obtained at each sampling area. A pressure index was used to assess the overall anthropogenic pressures acting in each estuary. Results showed that fish larvae were sensitive to water contamination, reducing the diversity and especially exhibiting a high dominance of few species. Furthermore, this study reinforced the idea that the high sensitivity of fish larvae can increase the accuracy of the environmental assessments when tackling short-time events of hydrological controls (physical barriers and control of the freshwater input), representing an advance in the water ecological quality assessments.