Plastics pollution has been recognized as a serious environmental problem. Nevertheless, new plastic uses, and applications are still increasing. Among these new applications, three-dimensional resin printers have increased their use and popularity around the world showing a vertiginous annual-sales growth. However, this technology is also the origin of residues generation from the alcohol cleaning procedure at the end of each printing. This alcohol/resin mixture can originate unintentionally very small plastic particles that usually are not correctly disposed, and as consequence, could be easily released to the environment. In this work, the nanoparticle generation from 3D printer’s cleaning procedure and their physicochemical characterization is reported. Nano-sized plastic particles are easily formed when the resin residues are dissolved in alcohol and placed under UV radiation from sunlight. These nanoparticles can agglomerate in seawater showing an average hydrodynamic diameter around 1 μm, whereas the same nanoparticles remain dispersed in ultrapure water, showing a hydrodynamic diameter of ≈300 nm. The formed nanoparticles showed an isoelectric point close to pH 2, which can facilitate their interaction with other positively charged pollutants. Thus, these unexpected plastic nanoparticles can become an environmental issue and public health risk.

Air pollution is a major public health challenge in the highly urbanized megacities of China. However, knowledge on exposure to ambient unregulated air pollutants such as black carbon (BC) and ultrafine particles (UFP) among the Chinese population, especially among urban high school students who may have highly variable time-activity patterns, is scarce. To address this, the personal exposures to BC and UFP of high school students (aged 17 to 18) in Chengdu, China were measured at 1-min intervals via portable samplers. Monitoring lasted for 2 consecutive 24-h periods with days classified as “school days” or “non-school days”. Time-activity diaries and measurements were combined to explore spatial, temporal, and behavioral factors that contribute to different exposure profiles. The overall geometric means of BC and UFP were 3.60 μg/m³ and 1.83 × 10⁴p/cm³, respectively with notable spatiotemporal variation in exposures observed. In general, the household and transport microenvironments were the predominant contributors to total BC (74.5%) and UFP (36.5%) exposure. However, the outdoor public microenvironment was found to have significantly higher overall average levels of BC than the household and transport microenvironments (p < 0.001) while also presenting the greatest exposure dose intensity (EDI – a measure of exposure in a microenvironment in proportion to time spent in that environment) of 4.79. The largest overall average level of UFP occurred in the indoor public microenvironment followed by transport. The outdoor public microenvironment also presented the greatest EDI of UFP (4.17). This study shows notable spatiotemporal variety in exposure patterns and will inform future exposure and population health studies. The high EDI outdoors may mean that health positive activities, such as exercise, may be being undermined by ambient pollution.

The main objectives of the present study were to investigate urinary monohydroxylated polycyclic aromatic hydrocarbons (OH-PAHs) in 77 primiparas who live in Shenzhen, Guangdong Province, China, and their association with 8-hydroxy-2′-deoxyguanosine (8-OHdG) and human health risks. High detection frequencies of OH-PAHs demonstrated the wide occurrence of chemicals in the human exposure to PAHs. The urinary concentrations of Σ₇OH-PAHs ranged from 1.37 to 45.5 ng/mL, and the median concentrations of 1-hydroxynaphthalene (1-OHN), 2-hydroxynaphthalene (2-OHN), 2-hydoxyfluorene (2-OHF), ΣOHPhe (the sum of 1-, 2+ 3-hydroxyphenanthrene), and 1-hydroxypyrene (1-OHP) were 3.00, 2.58, 0.31, 0.44, and 0.51 ng/mL, respectively. In the sum concentration of seven OH-PAHs, 1-OHN accounted for the largest proportion (43.7% of Σ₇OH-PAHs), followed by 2-OHN (37.1%), 2-OHF (4.94%), 1-OHP (8.01%), 1-OHPhe (4.79%), and 2+3-OHPhe (1.46%). The present results showed that vehicle exhaust and petrochemical emission are the main sources of PAHs in primiparas in Shenzhen, and inhalation is the most important exposure route. The living conditions have a significant influence on human exposure to PAHs. The concentrations of 8-OHdG were positively correlated with OH-PAH concentrations in urine because evidence suggested that urinary 8-OHdG levels can be considered as a biomarker of oxidative DNA damage. Hazard quotient was used to assess the human health risks from exposure to single compound, and hazard index was used to assess the cumulative risks of the compounds, which demonstrated that the exposure risks from PAHs in primiparas were relatively low.

In most cases, honey bees experience pesticide pollution in a long-term period through direct or indirect exposure, such as the development process from larvae to the pre-harvest stage. At present, little is known about how honey bees respond to pesticide stresses during the continuous development period. This study aims to examine effects of long-term acetamiprid exposure on the development and survival of honey bees, and further present the expression profile in larvae, 1-day-old, and 7-day-old adult worker bees that related to immune, detoxification, acetylcholinesterase (AChE) and memory. Honey bees from 2-day-old larvae to 14-day-old adults except the pupal stage were continuously fed with different acetamiprid solutions (0, 5, and 25 mg/L). We found that acetamiprid over 5 mg/L disturbed the development involving birth weight and emergence rate of newly emerged bees, and reduced the proportion of capped cells of larvae at 25 mg/L; gene expression related to immune and detoxification of worker bees exposed to acetamiprid was roughly activated, returned and then inhibited from larval to emerged and to the late adult stage, respectively. Moreover, lifespans of bees treated with acetamiprid at 25 mg/L were significantly reduced. The present study reflects the potential risk for honey bees continuously exposed to acetamiprid in the development stage.

Struvite (MgNH₄PO₃·6H₂O) crystallization is one of important methods of phosphorus recovery from wastewater. As to livestock wastewater, the high-strength occurrence of antibiotics and antibiotic resistance genes might induce struvite recovery to spread antibiotic resistance to the environment. However, limited information has been reported on the simultaneous transport of antibiotics and ARGs in struvite recovery. In the present study, tetracyclines (TCs) and tetracyclines antibiotic resistance genes (ARGs) were selected as the targeted pollutants, and their discrepant residues in struvite recovery from swine wastewater were investigated. TCs and ARGs were obviously detected, with their contents of 4.88–79.5 mg/kg and 6.99 × 10⁷–2.14 × 10¹¹ copies/g, notably higher than those of TCs 0.550–1.94 mg/kg and ARGs 3.98 × 10⁴–5.66 × 10⁷ copies/g obtained from synthetic wastewater. The correlational relationship revealed that predominant factors affecting TCs and ARGs transports were different. Results from network analyses indicated that among the total edges, the negative correlations between TCs and ARGs predominately occupied 18.0%. The redundancy analysis revealed that mineral components in the recovered products, including struvite, K-struvite and amorphous calcium phosphate, coupling with organic contents, displayed insignificant roles on TCs residues, where heavy metals exerted positive and remarkable functions to boost TCs migration. Unexpectedly, mineral components and heavy metals did not displayed significant promotion on ARGs transport as a whole.

With the development of modern industry, the problem of cadmium (Cd) pollution cannot be ignored and its toxicity has caused great personal injury to humans. Poly (ADP-ribose) polymerase 1 (PARP-1) protein is a research hotspot in recent years, the research we have published shows that 5 μM of Cd-treated NRK-52E cells activated PARP-1, but the specific effects of PARP-1 on DNA damage and cell cycle is unclear. Therefore, the purpose of this study is to reveal the effect of Cd on DNA damage and cell cycle arrest in NRK-52E cells, in addition, to investigate the role of PARP-1 in mediating this effect. Western blotting, comet assay, QRT-PCR, immunofluorescence, and co-immunoprecipitation were used to detect DNA damage and cell cycle-associated protein expression. Flow cytometry was used to assess cell cycle distribution and the apoptosis rates. Results showed that after the increase in treatment time and Cd concentration, the degree of DNA damage was significantly increased, and a transition from G0/G1 to S phase arrest was observed. In addition, inhibition of PARP-1 expression exacerbated cell damage and cell cycle arrest when DNA damage was low, but attenuated cell damage and even cell cycle arrest when DNA damage was severe. These findings in this study indicate that Cd causes DNA damage in NRK-52E cells, leading to cell cycle arrest at different phases depending on the degree of DNA damage. Moreover, PARP-1 plays an important role in mediating this effect, when DNA damage is low, it functions in DNA repair, however, when DNA damage is severe, it aggravates cell damage and induces cell death.

A microbially facilitated approach was developed to degrade 2, 2′, 4, 4′-tetrabrominated diphenyl ether (BDE-47). This approach consisted of biological production of Fe(II) and H₂O₂ by the dissimilatory metal-reducing bacterium Shewanella oneidensis MR-1 during the repetitive anoxic/oxic cycles and abiotic production of hydroxyl radical (HO●) with the biologically produced Fe(II) and H₂O₂ via Fenton reaction. Under the condition tested, BDE-47 did not inhibit the growth of S. oneidensis MR-1. Water soluble Fe(III)-citrate and the solid minerals ferrihydrite [Fe(III)₂O₃•0.5H₂O] and goethite [Fe(III)OOH] were tested in this study. Under anoxic condition, the amounts of Fe(II) produced by S. oneidensis MR-1 varied among the Fe(III)s tested, which decreased in the order of Fe(III)-citrate > ferrihydrite > goethite. Under subsequent oxic condition, H₂O₂ was produced via O₂ reduction by S. oneidensis MR-1. The amounts of H₂O₂ detected also varied, which decreased in the order of the reactions with Fe(III)-citrate > goethite > ferrihydrite. S. oneidensis MR-1 maintained its ability to produce Fe(II) and H₂O₂ for up to seven anoxic/oxic cycles. At each end of anoxic/oxic cycle, HO● was detected. The amount of HO● produced decreased in the order of the reactions with ferrihydrite > goethite > Fe(III)-citrate, which was opposite to that of H₂O₂ detected. Compared to the controls without HO●, the amounts of BDE-47 in the reactions with HO● decreased. The more HO● in the reaction, the less amount of BDE-47 detected. Furthermore, no BDE-47 degradation was observed when HO● was scavenged or ferrihydrite was either omitted or replaced by nitrate. Finally, identification of degradation products, such as hydroxylated BDE-47 and trisBDE, dibromophenol and monobromophenol, suggested that OH-addition and Br-substitution by HO● were the main mechanisms for degrading BDE-47. Collectively, all these results demonstrated for the first time that the Fenton reaction driven by S. oneidensis MR-1 degraded BDE-47 effectively.

The Atrato watershed is a rainforest that supports exceptional wildlife species and is considered one of the most biodiversity-rich areas on the planet, currently threatened by massive gold mining. Aimed to protect this natural resource, the Constitutional Court of Colombia declared the river subject to rights. The objective of this study was to quantify trace elements in sediments and fish from Atrato watershed, assessing their environmental and human health risk. Forty-two trace elements were quantified using ICP-MS. Thirty-one elements increased their concentration downstream the river. Concentration Factors (CF) suggest sediments were moderately polluted by Cr, Cu, Cd, and strongly polluted by As. Most stations had Cr (98%) and Ni (78%) concentrations greater than the Probable Effect Concentration (PEC) criteria. Together, toxic elements generate a Pollution Load Index (PLI) and a Potential Ecological Risk Index (RI) that categorized 54% of the sediments as polluted, and 90% as moderate polluted, respectively. Hemiancistrus wilsoni, a low trophic guild fish species, had the greater average levels for Ni, Cu, As and Cd, among other elements. Rubidium and Cs showed a positive correlation with fish trophic level, suggesting these two metals biomagnify in the food chain. The Hazard Quotient (HQ) for As was greater than 1 for several species, indicating a potential risk to human health. Collectively, data suggest gold mining carried out in this biodiversity hotspot releases toxic elements that have abrogated sediment quality in Atrato River, and their incorporation in the trophic chain constitutes a large threat on environmental and human health due to fish consumption. Urgent legal and civil actions should be implemented to halt massive mining-driven deforestation to enforce Atrato River rights.

A total of 74 high volume air samples were collected at a background site in Czech Republic from 2012 to 2014 in which the concentrations of 20 per- and polyfluoroalkyl substances (PFASs) were investigated. The total concentrations (gas + particle phase) ranged from 0.03 to 2.08 pg m⁻³ (average 0.52 pg m⁻³) for the sum of perfluoroalkyl carboxylic acids (∑PFCAs), from 0.02 to 0.85 pg m⁻³ (average 0.28 pg m⁻³) for the sum of perfluoroalkyl sulfonates (ΣPFSAs) and from below detection to 0.18 pg m⁻³ (average 0.05 pg m⁻³) for the sum of perfluorooctane sulfonamides and sulfonamidoethanols (ΣFOSA/Es). The gas phase concentrations of most PFASs were not controlled by temperature dependent sources but rather by long-range atmospheric transport. Air mass backward trajectory analysis showed that the highest concentrations of PFASs were mainly originating from continental areas. The average particle fractions (θ) of ΣPFCAs (θ = 0.74 ± 0.26) and ΣPFSAs (θ = 0.78 ± 0.22) were higher compared to ΣFOSA/Es (θ = 0.31 ± 0.35). However, they may be subject to sampling artefacts. This is the first study ever reporting PFASs concentrations in air samples collected over consecutive years. Significant decreases in 2012–2014 for PFOA, MeFOSE, EtFOSE and ∑PFCAs were observed with apparent half-lives of 1.01, 0.86, 0.92 and 1.94 years, respectively.

Microfibres are one of the most ubiquitous particulate pollutants, occurring in all environmental compartments. They are often assumed to be microplastics, but include natural as well as synthetic textile fibres and are perhaps best treated as a separate class of pollutants given the challenges they pose in terms of identification and contamination. Microfibres have been largely ignored by traditional methods used to sample floating microplastics at sea, which use 300–500 μm mesh nets that are too coarse to sample most textile fibres. There is thus a need for a consistent set of methods for sampling microfibres in seawater. We processed bulk water samples through 0.7–63 μm filters to collect microfibres in three ocean basins. Fibre density increased as mesh size decreased: 20 μm mesh sampled 41% more fibres than 63 μm, and 0.7 μm filters sampled 44% more fibres than 25 μm mesh, but mesh size (20–63 μm) had little effect on the size of fibres retained. Fibre density decreased with sample volume when processed through larger mesh filters, presumably because more fibres were flushed through the filters. Microfibres averaged 2.5 times more abundant at the sea surface than in water sampled 5 m sub-surface. However, the data were noisy; counts of replicate 10-L samples had low repeatability (0.15–0.36; CV = 56%), suggesting that single samples provide only a rough estimate of microfibre abundance. We propose that sampling for microfibres should use a combination of <1 μm and 20–25 μm filters and process multiple samples to offset high within-site variability in microfibre densities.