Metal-resistant bacteria can survive exposure to high metal concentrations without any negative impact on their growth. Biosorption is considered to be one of the more effective detoxification mechanisms acting in most bacteria. However, molecular-scale characterization of metal biosorption by wild metal-resistant bacteria has been limited. In this study, the Pb(II) biosorption behavior of Serratia Se1998 isolated from Pb-contaminated soil was investigated through macroscopic and microscopic techniques. A four discrete site non-electrostatic model fit the potentiometric titration data best, suggesting a distribution of phosphodiester, carboxyl, phosphoryl, and amino or hydroxyl groups on the cell surface. The presence of these functional groups was verified by the attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy, which also indicated that carboxyl and phosphoryl sites participated in Pb(II) binding simultaneously. The negative enthalpy (−9.11 kJ mol−1) and large positive entropy (81.52 J mol−1 K−1) of Pb(II) binding with the bacteria suggested the formation of inner-sphere complexes by an exothermic process. X-ray absorption fine structure (XAFS) analysis further indicated monodentate inner-sphere binding of Pb(II) through formation of C−O−Pb and P−O−Pb bonds. We inferred that C−O−Pb bonds formed on the flagellar surfaces, establishing a self-protective barrier against exterior metal stressors. This study has important implications for an improved understanding of metal-resistance mechanisms in wild bacteria and provides guidance for the construction of genetically engineered bacteria for remediation purposes.

Inhalation exposure to atmospheric polycyclic aromatic hydrocarbons (PAHs) is posing a great threat to human health. Biomass combustion in rural areas contributes greatly to the total PAH emission in China. To conduct a comprehensive risk assessment of ambient PAHs in rural China, a nationwide air sampling campaign was carried out in this study. The 16 U.S. Environmental Protection Agency priority PAHs in tree bark, which was employed as a passive air sampler, were analyzed. The summation of the 16 PAHs ranged from 11.7 to 12,860 ng/m³ in the air of rural China. The national median benzo(a)pyrene equivalent (BaPₑq) concentration was 18.4 ng/m³, with the range from 0.334 to 2497 ng/m³. The total inhalation carcinogenic risks of individual PAHs, with the exception for naphthalene, were very low (<1 × 10⁻⁶) at most of the sampling sites. The national median excess lifetime lung cancer risk associated with inhalation exposure to atmospheric PAHs was 20.3 × 10⁻⁶, corresponding to a population attributable fraction (PAF) of 3.38‰. Our estimations using tree bark were comparable to those reported in other studies and the uncertainties of the variables in the dataset were within the acceptable levels, demonstrating that tree bark is feasible for assessing the atmospheric PAH pollution and associated health risks. We feel that the outputs from this study can assist decision-makers focusing on protecting human health against exposure to atmospheric PAHs in rural China.

Using a realistic and environmental relevant approach, the present study aimed at understanding the biochemical and physiological basis of glyphosate (GLY)-induced stress in non-target plant species, using tomato (Solanum lycopersicum L.) as a model. For this purpose, plants were grown for 28 days under different concentrations of a commercial formulation of GLY (Roundup® UltraMax) - 0, 10, 20 and 30 mg kg⁻¹ soil. The exposure of plants to increasing concentrations of GLY caused a severe inhibition of growth (root and shoot elongation and fresh weight), especially in the highest treatments. In what regards the levels of reactive oxygen species (ROS), both hydrogen peroxide (H₂O₂) and superoxide anion (O₂.⁻) remained unchanged in shoots, but significantly increased in roots. Moreover, a concentration-dependent decrease in lipid peroxidation (LP) was found in shoots, though in roots differences were only found for the highest concentration of GLY. The evaluation of the antioxidant system showed that GLY interfered with several antioxidant metabolites (proline, ascorbate and glutathione) and enzyme activities (superoxide dismutase – SOD; catalase – CAT; ascorbate peroxidase – APX), generally inducing a positive response of the defense mechanisms. Overall, data obtained in this study unequivocally demonstrated that soil contamination by GLY, applied as part of its commercial formulation Roundup® UltraMax, impairs the growth and physiological performance of tomato plants, and likely of other non-target plant species, after 28 days of exposure by clearly affecting the normal redox homeostasis.

Recent studies have demonstrated the occurrence of microplastic fibers (MFs) in soil environments. To determine whether MFs are harmful for soil biota, we evaluated toxic effects on terrestrial snails (Achatina fulica) after 28 d exposure to polyethylene terephthalate MFs at concentrations of 0.01–0.71 g kg−1 (dry soil weight). Digestion kinetics experiments on 24 snails showed that MFs can be ingested and excreted within 48 h. We found the appearance of cracks and deterioration on the surface of MFs after depuration by the digestive system. Prolonged exposure to 40 snails showed that 0.14–0.71 g kg−1 MFs caused an average reduction of 24.7–34.9% food intake and 46.6–69.7% excretion. 0.71 g kg−1 MFs induced significant villi damage in the gastrointestinal walls of 40% snails, but did not influence the histology of the liver and kidney. Moreover, 0.71 g kg−1 MFs exposure reduced glutathione peroxidase (59.3 ± 13.8%) and total antioxidant capacity (36.7 ± 8.5%), but elevated malondialdehyde level (58.0 ± 6.4%) in the liver, which indicates oxidative stress is involved in the toxic mechanism. Our results suggest that MFs have adverse impacts on the fitness of soil organisms, and highlight the ecological risks of microplastic pollution in terrestrial ecosystems.

The present study investigated the beneficial role of selenium-nanoparticles (Se-NPs) in mitigating the adverse effects of soil-salinity on growth and yield of strawberry (Fragaria × ananassa Duch.) plants by maneuvering physiological and biochemical mechanisms. The foliar spray of Se-NPs (10 and 20 mg L⁻¹) improved the growth and yield parameters of strawberry plants grown on non-saline and different saline soils (0, 25, 50 and 75 mM NaCl), which was attributed to their ability to protect photosynthetic pigments. Se-NPs-treated strawberry plants exhibited higher levels of key osmolytes, including total soluble carbohydrates and free proline, compared with untreated plants under saline conditions. Foliar application of Se-NPs improved salinity tolerance in strawberry by reducing stress-induced lipid peroxidation and H₂O₂ content through enhancing activities of antioxidant enzymes like superoxide dismutase and peroxidase. Additionally, Se-NPs-treated strawberry plants showed accumulation of indole-3-acetic acid and abscisic acid, the vital stress signaling molecules, which are involved in regulating different morphological, physiological and molecular responses of plants to salinity. Moreover, the enhanced levels of organic acids (e.g., malic, citric and succinic acids) and sugars (e.g., glucose, fructose and sucrose) in the fruits of Se-NPs-treated strawberry plants under saline conditions indicated the positive impacts of Se-NPs on the improvement of fruit quality and nutritional values. Our results collectively demonstrate the definite roles of Se-NPs in management of soil salinity-induced adverse effects on not only strawberry plants but also other crops.

Atmospheric stability significantly influences the accumulation and dispersion of air pollutants in the near-surface atmosphere, yet few stability metrics have been applied as predictors in statistical PM₂.₅ concentration mapping practices. In this study, eleven stability metrics were derived from radiosonde soundings collected in eastern China for the time period of 2015–2018 and then applied as independent predictors to explore their potential in favoring the prediction of PM₂.₅. The statistical results show that the in situ PM₂.₅ concentration measurements correlated well with these stability metrics, especially at monthly and seasonal timescales. In contrast, correlations at the daily timescale differed markedly between stability metric and also varied with seasons. Nevertheless, the modeling results indicate that incorporating these stability metrics into the PM₂.₅ modeling framework rendered small contribution to PM₂.₅ prediction accuracy, yielding an increase of R² by < 5% and a reduction of RMSE by < 1 μg/m³ on average. Compared with other stability indices, the inversion depth and intensity appeared to have relative larger benefiting potential. In general, our findings indicate that including these stability metrics would not result in significant contribution to the PM₂.₅ prediction accuracy in eastern China since their effects could be partially overwhelmed or offset by other predictors such as AOD and boundary layer height.

Anaerobic ammonium oxidation (anammox) is recognized as an important bioprocess for nitrogen removal, yet little is known about the associated microbial communities in urban river networks which are intensively disturbed by human activity. In the present study, we investigated the community composition and abundance of anammox bacteria in the urban river network of Shanghai, and explored their potential correlations with nitrogen removal activities and the environmental parameters. High biodiversity of anammox bacteria was detected in the sediment of urban river networks, including Candidatus Brocadia, Scalindua, Jettenia, and Kuenenia. Anammox bacterial abundance ranged from 3.7 × 10⁶ to 3.9 × 10⁷ copies g⁻¹ dry sediment based on 16S rRNA gene, which was strongly correlated to the metabolic activity of anammox bacteria (P < 0.01). A strong linkage between anammox bacteria and denitrifiers was detected (P < 0.05), implying a potential metabolic interdependence between these two nitrogen-removing microbes was existed in urban river networks. Sediment ammonium (NH₄⁺) made a significant contribution to the anammox bacterial community-environment relationship, while anammox bacterial abundance related significantly with sediment total organic carbon (TOC) and silt contents (P < 0.05). However, no statistically significant correlation was observed between cell-specific anammox rate and the measured environmental factors (P > 0.05). In general, the community composition and abundance of anammox bacteria in different hierarchies of the river network was homogeneous, without significant spatial variations (P > 0.05). These results provided an opportunity to further understand the microbial mechanism of nitrogen removal bioprocesses in urban river networks.

Once released into the environment, engineered nanomaterials can significantly influence the transformation and fate of organic contaminants. To date, the abilities of composite nanomaterials to catalyze environmentally relevant abiotic transformation reactions of organic contaminants are largely unknown. Herein, we investigated the effects of two nanocomposites – consisting of anatase titanium dioxide (TiO2) with different predominantly exposed crystal facets (i.e., {101} or {001} facets) anchored to hydroxylated multi-walled carbon nanotubes (OH-MWCNT) – on the hydrolysis of 1,1,2,2-tetrachloroethane (TeCA), a common groundwater contaminant, at ambient pH (6, 7 and 8). Both OH-MWCNT/TiO2 nanocomposites were more effective in catalyzing the dehydrochlorination of TeCA than the respective component materials (i.e., bare OH-MWCNT and bare TiO2). Moreover, the synergistic effect of the two components was evident, in that the incorporation of OH-MWCNT increased the TeCA adsorption capacity of the nanocomposites, significantly enhancing the catalytic effect of the deprotonated hydroxyl and carboxyl groups on nanocomposite surfaces, which served as the main catalytic sites for TeCA hydrolysis. The findings may have important implications for the understanding of the environmental implications of composite nanomaterials and may shed light on the design of high-performance nanocomposites for enhanced contaminant removal.