Ecotoxicological studies show the association between pesticide pollution and transgenerational toxicity in aquatic organisms. However, a less considered risk is that many pesticides can be metabolized and transferred to offspring as new toxicants. In this study, we used zebrafish to evaluate the maternal transfer risk of fipronil (FIP), which is a great threat to aquatic organisms with toxic metabolite formation. After 28-day exposure to environmentally relevant concentrations (1.0, 5.0 and 10.0 μg/L) of FIP in adult female zebrafish (F0), the toxicants off-loading and transgenerational toxicity in offspring were studied. High burdens of FIP and its sulfone metabolite were found in both F0 and the embryos (F1), resulting in increased CYP450 activity. The residual levels of the metabolite were higher than those of the parent compound. Chiral analysis further showed a preferential accumulation of S-enantiomer of FIP in both F0 and F1. Maternal exposure to FIP increased the malformation rate and decreased the swim speed in larvae. Additionally, after exposure, the levels of thyroid hormones (THs), including triiodothyronine (T3) and thyroxine (T4), decreased in both generations, particularly in the F1. Gene transcription expression along the hypothalamic-pituitary-thyroid (HPT) axis was also significantly affected. Maternal exposure to FIP increased sulfone metabolite enrichment and cause multiple toxic effects in F1. Findings from this study highlight the key role of biologically active product formation in the maternal transfer of pollutants and associated risk assessment.

Aircraft soot has a significant impact on global and local air pollution and is of particular concern for the population working at airports and living nearby. The morphology and chemistry of soot are related to its reactivity and depend mainly on engine operating conditions and fuel-type. We investigated the morphology (by transmission electron microscopy) and chemistry (by X-ray micro-spectroscopy) of soot from the exhaust of a CFM 56-7B26 turbofan engine, currently the most common engine in aviation fleet, operated in the test cell of SR Technics, Zurich airport. Standard kerosene (Jet A-1) and a biofuel blend (Jet A-1 with 32% HEFA) were used at ground idle and climb-out engine thrust, as these conditions highly influence air quality at airport areas. The results indicate that soot reactivity decreases from ground idle to climb-out conditions for both fuel types. Nearly one third of the primary soot particles generated by the blended fuel at climb-out engine thrust bear an outer amorphous shell implying higher reactivity. This characteristic referring to soot reactivity needs to be taken into account when evaluating the advantage of HEFA blending at high engine thrust. The soot type that is most prone to react with its surrounding is generated by Jet A-1 fuel at ground idle. Biofuel blending slightly lowers soot reactivity at ground idle but does the opposite at climb-out conditions. As far as soot reactivity is concerned, biofuels can prove beneficial for airports where ground idle is a common situation; the benefit of biofuels for climb-out conditions is uncertain.

The application of low ozone dosage to minimize the problems caused by filamentous foaming was evaluated in two bioreactors of an urban wastewater treatment plant. Filamentous and nitrifying bacteria, as well as protist and metazoa, were monitored throughout a one-year period by FISH and conventional microscopy to examine the effects of ozone application on these specific groups of microorganisms. Multivariate data analysis was used to determine if the ozone dosage was a key factor determining the low carbon and nitrogen removal efficiencies observed throughout the study period, as well as to evaluate its impact on the biological communities monitored. The results of this study suggested that ozonation did not significantly affect the COD removal efficiency, although it had a moderate effect on ammonia removal efficiency. Filamentous bacteria were the community most influenced by ozone (24.9% of the variance explained by ozone loading rate), whilst protist and metazoa were less affected (11.9% of the variance explained). Conversely, ozone loading rate was not a factor in determining the nitrifying bacterial community abundance and composition, although this environmental variable was correlated with ammonia removal efficiency. The results of this study suggest that different filamentous morphotypes were selectively affected by ozone.

Aromatic halogenated chemicals are an unregulated class of byproducts (DBPs) generated from disinfection processes in the water environment. Information on the toxicological interactions, such as antagonism and synergism, present in DBP mixtures remains limited. This study aimed to determine the toxicological effects of aromatic halogenated DBP mixtures on the freshwater bacterium Vibrio qinghaiensis sp.-Q67. The acute toxicities of seven DBPs and their binary mixtures toward V. qinghaiensis sp.-Q67 were determined through microplate toxicity analysis. The toxicities of single DBPs were ranked as follows: 2,5-dibromohydroquinone > 2,4-dibromophenol > 4-bromo-2-chlorophenol ≈ 2,6-dibromo-4-nitrophenol > 2,6-dichloro-4-nitrophenol > 2-bromo-4-chlorophenol > 4-bromophenol. The percentages of synergism (experimental values higher than the predicted concentration addition) on the levels of 50%, 20%, and 10% effective concentrations reached 61%, 41%, and 31%, respectively. These results indicated that the probability of synergism decreased as concentration levels decreased. The synergetic effects of the compounds were dependent on concentration levels and concentration ratios. The proposed quantitative structure–activity relationship model can be used to predict the interactive toxicities exerted by 105 binary DBP mixture rays of 21 DBP mixture systems.

Anthropogenic activities including coastal industries, urbanization, extensive agriculture and aquaculture as well as their cumulative impacts represent major sources of perturbation of marine coastal systems. Macrobenthic communities are useful ecological indicators for monitoring the health status of marine environments (or polluted environments). The present study reports, for the first time, the response of benthic macrofauna sampled during two years survey (2015–2016) to multiple anthropogenic pressures on the coastal zone south of Sfax (Tunisia). A total of 12 stations were monitored seasonally at locations downstream from the main potential sources of disturbance. 106 macrobenthos taxa, belonging to six animal phyla and 70 families, were identified with a dominance of polychaetes (42%), crustaceans (35%) and molluscs (18%). We used an ANOVA test and cluster analysis to identify spatial gradient linked to environmental and anthropogenic factors, including depth, sedimentary texture and anthropogenic activities (i.e. phosphogypsum discharges).The macrofauna present lowest species number and abundance on stations undergoing anthropogenic inputs, which are extremely polluted by heavy metals (Cd, Cu, F and N) and excess of organic matter. Univariate parameters reveal a general trend of increasing species diversity with increasing distance from the pollution source. The polluted stations are strongly dominated by carnivores, and selective deposit feeders, and more closely linked to the availability of trophic resources than to anthropogenic constraints. The seasonal changes in macrobenthic abundance, diversity indices and community structure are mainly linked to the biological cycle (e.g. recruitment events) of the dominant species. Biotic indices (AMBI and BO2A) classified the coastal zone south of Sfax as moderate and good ecological status. This study suggests that initiating a long-term monitoring programme would improve our understanding of the temporal changes of macrobenthic communities of this ecosystem, contributing to the assessment of effective management and conservation measures in this disturbed area.

Air pollution is one of the leading preventable threats to public health. Emerging evidence indicates that exposure to environmental stressors is associated with abnormal foetal development. However, how prenatal exposure to diesel exhaust PM2.5 (DEP) predisposes adult offspring to the development of non-alcoholic fatty liver disease (NAFLD) remains unclear. To examine this, C57BL/6J mice were exposed to DEP or a vehicle before conception and during pregnancy and fed normal chow or a high-fat diet. Then, the hepatic fatty accumulation in the adult male offspring and possible molecular mechanisms were assessed. Our data showed that prenatal exposure to DEP on normal chow led to hepatic steatosis in adult male offspring with normal liver function. However, prenatal DEP exposure relieved the hepatic steatosis and liver function in offspring of mice fed a high-fat diet. Furthermore, prenatal exposure to DEP on normal chow increased lipogenesis and worsened fatty acid oxidation. The counteractive effect of prenatal DEP exposure on high-fat-diet-induced hepatic steatosis was produced through upregulated adenosine 5′-monophosphate-activated protein kinase, and this improved lipogenesis and fatty acid oxidation. Collectively, prenatal exposure to DEP programmed the development of NAFLD differently in the adult male offspring of mice fed normal chow and a high-fat diet, showing the pleotrophic effects of exposure to adverse environmental factors in early life.

In the study, the effects of dimethyl phthalate (DMP) on the antioxidant defense capacity and immune functions of human erythrocytes were experimentally explored. DMP affected the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) and the contents of glutathione (GSH) and malondialdehyde (MDA) in erythrocytes, thus impairing the function of antioxidant defense system of erythrocytes. When DMP concentration increased from 0 to 28 μmol L⁻¹, the SOD and GPX activities were increased firstly and then gradually decreased. When DMP concentration was below 20 μmol L⁻¹, the relative activity of SOD was enhanced by DMP and the effect was known as hormesis. The relative activity of GPX was also increased when the concentration of DMP was below 12 μmol L⁻¹. The CAT activity was more significantly inhibited by DMP than the activities of SOD and GPX, whereas the relative GSH content was increased by DMP. MDA levels were significantly changed after the exposure to DMP (0–24 μmol L⁻¹). The experimental results of the activity of SOD and CAT, and the content of MDA also suggested that DMP could inhibit the immune functions of red blood cells (RBCs), which were further proved by the decrease of two indicators (RBC-C₃b and RBC-IC) due to the destruction of C₃b receptor with immune adherence function on erythrocyte membrane. The study provides a deep understanding of the toxicity of DMP on erythrocytes.

Excessive use of nitrogenous fertilizers and their improper management in agriculture causes nitrate contamination of surface and groundwater resources. This study was conducted along the seasonally flooded alluvial agricultural area of Indus River Basin to determine the spatial and temporal dynamics of nitrate concentrations in the groundwater along the river. Total of 112 samples were collected from shallow (30–40 ft) and deep groundwater (120–150 ft) wells at seven sites, 25 km apart from each other and covered an area of 170 km along the river, during four sampling campaigns between October 2016 to May 2017 i.e. in start, mid and end of dry season. The study period covered the whole agricultural cycle including the wet summer season with no agricultural activities under flooding and the sampling sites were always less than 2 km from the river bank. Nitrate concentrations of shallow wells were 15–54 and 20–45 mg L⁻¹ during the start and middle of dry season, respectively. However, at the end of the dry season, the highest nitrate concentrations of 35–75 mg L⁻¹ were recorded and 70% of these samples contained nitrate concentrations above the permissible limit 50 mg L⁻¹. Similar seasonal patterns of nitrate concentrations were observed in deep wells, however, δ¹⁸O data suggested lower recharge in deep well than shallow wells. The results illustrated that high nitrate concentrations in shallow wells were associated with high δ¹⁸O values indicating that the quantity of evaporated water infiltrated from the floodplain, possibly from distribution channels, along with the nitrate polluting shallow wells more than the deep wells. At the end of the dry season, nitrate concentrations exceeded the permissible limits in both shallow and deep wells, which possibly happened due to the horizontal movement of groundwater along with the nitrate mixing during vertical seepage of river water to the aquifers.

The increase in ambient fine dust particles (FDP) due to urbanization and industrialization has been identified as a major contributor to air pollution. It has become a serious issue that threatens human health because it causes respiratory diseases and skin aging. In the present study, the protective effect of the green tea catechin, (−)-epigallocatechin gallate (EGCG), against FDP (ERM-CZ100)-stimulated skin aging in human dermal fibroblasts (HDFs) was investigated. The results demonstrate that EGCG significantly and dose-dependently scavenged intracellular reactive oxygen species (ROS) in and increased the viability of FDP-stimulated HDFs. In addition, EGCG dose-dependently recovered collagen synthesis and inhibited intracellular elastase and collagenase activities. Moreover, EGCG decreased the expression of human matrix metalloproteinases (MMPs) via regulation of nuclear factor kappa B (NF-κB), activator protein 1 (AP-1), and mitogen-activated protein kinases (MAPKs) signaling pathways in FDP-stimulated HDFs. This study suggests that EGCG is a potential anti-aging candidate that can be used for FDP-induced skin aging as a therapeutic agent itself or as an ingredient in pharmaceutical and cosmeceutical products.

Land degradation by salinization and sodification changes soil function, destroys soil health, and promotes bioaccumulation of heavy metals in plants, but little is known about their fundamental mechanisms in shaping microbial communities and regulating microbial interactions. In this study, we explored the impact of saline-alkaline (SA) stress on soil bacterial and fungal community structures in different Cd-contaminated soils of Dezhou, Baoding, Xinxiang, Beijing and Shenyang cities from the North China Plain, China. Increased soil salinity and alkalinity enhanced Cd availability, indicated by significant increases in available Cd2+ in soil solution of 34.1%–49.7%, soil extractable Cd of 32.0–51.6% and wheat root Cd concentration of 24.5%–40.2%, as well as decreased activities of antioxidative enzymes of wheat root when compared with CK (no extra neutral or alkaline salts added). Soil bacteria were more active in response to the SA stress than fungi, as the significant structural reorganization of soil bacterial microbiota rather than fungal microbiota between SA and CK treatments was illustrated by principal component analysis. Adding neutral and alkaline salts enriched oligotrophic and haloalkaliphilic taxa in the Sphingobacteriaceae, Cellvibrionaceae, and Caulobacteraceae bacterial families, but decreased some Acidobacteria such as subgroup 6_norank, which was a sensitive biomarker that responded only to Cd contamination in CK-treated soils. Conversely, fungi were more sensitive to soil differences than bacteria: the composition of the fungal community was significantly different among different soil types. Phylogenetic molecular ecological network (pMEN) analysis further indicated that the microbial community structure and network interactions were altered to strengthen the adaptability of microorganisms to SA stress; the changes in structure and network interactions were proposed to contribute to competitive interactions. Most of the keystone genera identified in SA-treated soils, such as Blastococcus, Gemmatimonas, RB41, or Candida, had relatively low abundances (<1%), indicating their disproportionate ecological roles in triggering resistance or tolerance to SA stress and Cd toxicity.