This study sampled six times of river water, sediment, and tilapia (Oreochromis niloticus) in the Dan-Shui River, Taipei, Taiwan; 10 feminizing compounds were analyzed using ultra-performance liquid chromatography-tandem mass spectrometry. Bisphenol A (508 ± 634 ng/L, geometric mean (GM) 303 ng/L) and nonylphenol (491 ± 570 ng/L, GM 328 ng/L) were the most abundant among analytes in the river water. Nonylphenol (770 ± 602 ng/g wet weight, GM 617 ng/g wet weight) was also the highest in sediment. Fish may uptake nonylphenol and nonylphenol ethoxylates from river water and sediment because there were significant correlations between the concentrations in these matrixes and those in fish tissues (r ₛ ranged from 0.21 to 0.49, p < 0.05). The bioaccumulation of nonylphenol, nonylphenol ethoxylates and bisphenol A in gonad, eggs, and liver was much higher than that in muscle (e.g. mean bioaccumulation factors of nonylphenol were 27,287, 20,971, 9,576 and 967, respectively) and might result in low liver fractions in fish body weights (0.66 % ± 0.39 %, GM 0.55 %) and the skewed sex ratio of fish (male to female = 0.52). This innovative study linked the environmental and internal doses statistically in the globally distributed wild fish by analyzing feminizing compounds in water, sediment, and four fish tissues including gonad and eggs.

Inhibition of nitrification by four typical pollutants (acrylonitrile, acrylic acid, acetonitrile and cyanide) in acrylonitrile wastewater was investigated. The inhibitory effect of cyanide on nitrification was strongest, with a 50% inhibitory concentration of 0.218 mg·gVSS⁻¹ being observed in a municipal activated sludge system. However, the performance of nitrification was recovered when cyanide was completely degraded. The nitrification, which had been inhibited by 4.17 mg·gVSS⁻¹ of free cyanide for 24 h, was recovered to greater than 95% of that without cyanide after 10 days of recovery. To overcome cyanide inhibition, cyanide-degrading bacteria were cultivated in a batch reactor by increasing the influent cyanide concentration in a stepwise manner, which resulted in an increase in the average cyanide degradation rate from 0.14 to 1.01 mg CN⁻·gVSS⁻¹·h⁻¹ over 20 days. The cultured cyanide-degrading bacteria were shaped like short rods, and the dominant cyanide-degrading bacteria strain was identified as Pseudomonas fluorescens NCIMB by PCR.

The low bioavailability of Pb and low number of Pb-tolerant plant species represent an important limitation for Pb phytoextraction. It was recently suggested that halophyte plant species may be a promising material for this purpose, especially in polluted salt areas while Pb mobility may be improved by synthetic chelating agents. This study aims to evaluate Pb extraction by the halophyte Sesuvium portulacastrum in relation to the impact of EDTA application. Seedling were cultivated during 60 days on Pb artificially contaminated soil (200, 400, and 800 ppm Pb) in the presence or in the absence of EDTA (3 g kg⁻¹soil). Results showed that upon to 400 ppm, Pb had no impact on plant growth. However, exogenous Pb induce a decrease in shoot K⁺while it increased shoot Mg²⁺and had no impact on shoot Ca²⁺concentrations. Lead concentration in the shoots increased with increasing external Pb doses reaching 1,390 ppm in the presence of 800 ppm lead in soil. EDTA addition had no effect on plant growth but strongly increased Pb accumulation in the shoot which increased from 1,390 ppm in the absence of EDTA to 3,772 ppm in EDTA-amended plants exposed to 800 ppm exogenous Pb. Both Pb absorption and translocation from roots to shoots were significantly enhanced by EDTA application, leading to an increase in the total amounts of extracted Pb per plant. These data suggest that S. portulacastrum is very promising species for decontamination of Pb²⁺-contaminated soil and that its phytoextraction potential was significantly enhanced by addition of EDTA to the polluted soil.

Selected water quality parameters and spectroscopic characteristics of dissolved organic matter (DOM) were examined during two different seasons for an artificial coastal lake (Shiwha Lake in South Korea), which are affected by seawater exchange due to the operation of a tidal power plant and external organic loadings from the upstream catchments. The coastal lake exhibited much lower concentrations of organic matter and nutrients than the upstream sources. The spectroscopic properties of the lake DOM were easily distinguished from those of the catchment sources as revealed by a lower absorption coefficient, lower degree of humification, and higher spectral slopes. The observed DOM properties suggest that the lake DOM may be dominated by smaller molecular size and less condensed structures. For the lake and the upper streams, higher absorption coefficients and fluorescence peak intensities but lower spectral slopes and humification index were found for the premonsoon versus the monsoon season. However, such seasonal differences were less pronounced for the industrial channels in the upper catchments. Three distinctive fluorophore groups including microbial humic-like, tryptophan-like, and terrestrial humic-like fluorescence were decomposed from the fluorescence excitation-emission matrix (EEM) of the DOM samples by parallel factor analysis (PARAFAC) modeling. The microbial humic-like component was the most abundant for the industrial channels, suggesting that the component may be associated with anthropogenic organic pollution. The terrestrial humic-like component was predominant for the upper streams, and its relative abundance was higher for the rainy season. Our principal component analysis (PCA) results demonstrated that exchange of seawater and seasonally variable input of allochthonous DOM plays important roles in determining the characteristics of DOM in the lake.

Maluan Bay, characterized by various degrees of anthropogenic contamination, is considered as one of the most industrialized and urbanized coastal lagoon in China, where large amounts of metal contaminants in surface water and biota were detected in previous studies. However, no clear discriminating power among sampling sites could be made only through comparisons between contaminant levels and Environmental Quality Standards and especially biological-based monitoring integrating biomarkers and bioaccumulation of exposure are scarce. For this purpose, antioxidants enzymes (superoxide dismutase, catalase, and glutathione peroxidase) and glutathione-S-transferase were assessed using the hepatopancreas of shrimp Litopenaeus vannamei after 7 days laboratory exposure under controlled conditions to characterize the effects of polluted waters to shrimps. The metal concentrations of sampled water and bioaccumulation in hepatopancreatic tissues were also analyzed, and data were linked to biomarkers’ responses by multivariate (principal component analysis–factor) analysis. A representation of estimated factor scores was performed to confirm the factor descriptions classifying the pollution status and characterizing the studied sites, which pointed out the impact of multiple sources of contaminants to the water quality and provided further evidences to the existence of clear pollution and toxicological gradients in critical areas. The results of the present investigation underlined that the integrated approach could be a powerful tool for the identification of causal toxic contaminants in complex mixtures and the assessment of human-induced environmental quality of the system in coastal zones.

The present study examined the effects of oral exposure of 4,4′-dibromodiphenyl ether (BDE-15), 4,4′-dichlorodiphenyl ether (CDE-15), and 4,4′-dihydroxydiphenyl ether (HODE-15) on hepatic oxidative stress (OS) and metal status in Institute of Cancer Research (ICR) male mice. Furthermore, the role of vitamin E in ameliorating potential OS caused by BDE-15, CDE-15, and HODE-15 was investigated. Three groups of mice were exposed to 1.20 mg/kg(body weight)/day of each of the three toxicants for 28 days. Results showed that none of the three toxicants altered growth rates of mice, but significantly increased (P < 0.05) relative liver weights and decreased relative kidney weights. Pathological changes including cell swelling, inflammation and vacuolization, and hepatocellular hypertrophy in livers were observed. Significant decreases (P < 0.05 and P < 0.01) in superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) activity, and glutathione (GSH) levels, together with increases in malondialdehyde (MDA) content were recorded in all toxicant-treated groups. Hepatic copper levels increased in all toxicant-treated groups. Hepatic zinc levels decreased in the liver of BDE-15-treated mice, whereas they increased in the livers of CDE-15-treated and HODE-15-treated mice. In conclusion, daily exposure to the three toxicants perturbed metal homeostasis and increased OS in mouse liver. Experimental data indicated the hepatic oxidative toxicity of the three toxicants followed the order BDE-15 < HODE-15 < CDE-15. Moreover, the study proved that daily supplementation of 50 mg/kg vitamin E is effective to ameliorate the hepatic OS status and metal disturbance in mice.

The objective of the study is to investigate the long-run relationship between climatic factors (i.e., greenhouse gas emissions, agricultural methane emissions, and industrial nitrous oxide emission), air pollution (i.e., carbon dioxide emissions), and energy sources (i.e., nuclear energy; oil, gas, and coal energy; and fossil fuel energy) in the panel of 35 developed countries (including EU-15, new EU member states, G-7, and other countries) over a period of 1975–2012. In order to achieve this objective, the present study uses sophisticated panel econometric techniques including panel cointegration, panel fully modified OLS (FMOLS), and dynamic OLS (DOLS). The results show that there is a long-run relationship between the variables. Nuclear energy reduces greenhouse gases and carbon emissions; however, the other emissions, i.e., agricultural methane emissions and industrial nitrous oxide, are still to increase during the study period. Electricity production from oil, gas, and coal sources increases the greenhouse gases and carbon emissions; however, the intensity to increase emissions is far less than the intensity to increase emissions through fossil fuel. Policies that reduce emissions of greenhouse gases can simultaneously alter emissions of conventional pollutants that have deleterious effects on human health and the environment.

The development and application of an analytical methodology for the pretreatment and determination of 253 multiclass pesticides, in lake sediment samples, using liquid chromatography coupled with mass spectrometry (LC-MS/MS) are described in this work. Sediments of lakes Volvi, Doirani, and Kerkini, located in northern Greece, were collected in two-time periods (fall/winter 2010 and spring/summer 2011) and analyzed, applying the developed analytical methodology. Microwave-assisted extraction (MAE) was applied to extract the pesticide residues from lake sediment samples. Analytical results were stored, categorized, and visualized using geographical information systems, in order to assess and observe spatial and temporal variations of the pollution. Main pesticides that were detected included the following: amitrole, tebuconazole, phoxim, diniconazole, sethoxydim, temephos, tetrachlorvinphos, pendimethalin, boscalid, disulfoton sulfone, lenacil, propiconazole, cycloxydim, pyridaben, and terbuthylazine. Amitrole, diniconazole, and tebuconazole were found to be common in all three lakes. Lakes Kerkini and Doirani exhibited increased concentrations during the first sampling period (winter 2010) with predominant pesticide classes, triazines/triazoles and organophosphates. Pollution is mainly located near the populated villages of the lakes and the nearby cultivations. During the second sampling period, pesticide concentrations appear lower and located in sediments near the center of the lake. Lake Volvi exhibits increased pesticide concentrations during the second sampling period, temporal and spatial variations and different pesticide profile pattern. Increased pollution occurs near the center of the lake during the first sampling period, mainly comprised by triazines/triazoles and organophosphates. During the second sampling period, the majority of the sediment samples demonstrated a different pesticide profile dominated by unclassified pesticides and triazines/triazoles. Mineralogical analysis of the samples demonstrates that sediments are mainly composed of clay, mud, and sand particles, and they present spatial variations. Near the center of the lakes, sediments appear to be more fine-grained with higher clay content and are more likely to adsorb pesticides.

This study focuses on the biodegradation of recalcitrant, coloured compounds resulting from auto-oxidation of Acid Orange 7 (AO7) in a sequential facultative anaerobic–aerobic treatment system. A novel mixed bacterial culture, BAC-ZS, consisting of Brevibacillus panacihumi strain ZB1, Lysinibacillus fusiformis strain ZB2, and Enterococcus faecalis strain ZL bacteria were isolated from environmental samples. The acclimatisation of the mixed culture was carried out in an AO7 decolourised solution. The acclimatised mixed culture showed 98 % decolourisation within 2 h of facultative anaerobic treatment using yeast extract and glucose as co-substrate. Subsequent aerobic post treatment caused auto-oxidation reaction forming dark coloured compounds that reduced the percentage decolourisation to 73 %. Interestingly, further agitations of the mixed culture in the solution over a period of 48 h significantly decolourise the coloured compounds and increased the decolourisation percentage to 90 %. Analyses of the degradation compounds using UV–visible spectrophotometer, Fourier transform infrared spectroscopy (FTIR) and high performance liquid chromatography (HPLC) showed complete degradation of recalcitrant AO7 by the novel BAC-ZS. Phytotoxicity tests using Cucumis sativus confirmed the dye solution after post aerobic treatment were less toxic compared to the parent dye. The quantitative real-time PCR revealed that E. faecalis strain ZL was the dominant strain in the acclimatised mix culture.

The 42nd congress of the “Groupe Français des Pesticides” (French Group of Pesticide research–GFP 2012) took place 30 May–1 June at the “École Nationale Supérieure d’Ingénieurs de Poitiers” (ENSIP), a French generalist engineering grande école in Poitiers. Its focus is on the protection of the environment and is part of the University of Poitiers, one of the oldest universities in Europe. GFP is an annual conference where the latest developments in the field of pesticide research are presented. The topics most commonly discussed mainly concern environmental quality, monitoring, fate, and transfer of pesticides in the environment or risks associated with use of pesticides, but every year a key theme sets the tone. For this 42nd edition, the theme “Innovative strategies for plant protection” was retained.