This study deals with the evaluation of water quality of the Three Gorges Reservoir (TGR) in order to assess its suitability as a raw water source for drinking water production. Therefore, water samples from (1) surface water, (2) tap water, and (3) wastewater treatment plant effluents were taken randomly by 2011-2012 in the area of the TGR and were analyzed for seven different organic contaminant groups (207 substances in total), applying nine different analytical methods. In the three sampled water sources, typical contaminant patterns were found, i.e., pesticides and polycyclic aromatic hydrocarbons (PAH) in surface water with concentrations of 0.020-3.5 μg/L and 0.004-0.12 μg/L, disinfection by-products in tap water with concentrations of 0.050-79 μg/L, and pharmaceuticals in wastewater treatment plant effluents with concentrations of 0.020-0.76 μg/L, respectively. The most frequently detected organic compounds in surface water (45 positives out of 57 samples) were the pyridine pesticides clopyralid and picloram. The concentrations might indicate that they are used on a regular basis and in conjunction in the area of the TGR. Three- and four-ring PAH were ubiquitously distributed, while the poorly soluble five- and six-ring members, perfluorinated compounds, polychlorinated biphenyls, and polybrominated diphenyl ethers, were below the detection limit. In general, the detected concentrations in TGR are in the same range or even lower compared to surface waters in western industrialized countries, although contaminant loads can still be high due to a high discharge. With the exception of the two pesticides, clopyralid and picloram, concentrations of the investigated organic pollutants in TGR meet the limits of the Chinese Standards for Drinking Water Quality GB 5749 (Ministry of Health of China and Standardization Administration of China 2006) and the European Union (EU) Council Directive 98/83/EC on the quality of water intended for human consumption (The Council of the European Union 1998), or rather, the EU Directive on environmental quality standards in the field of water policy (The European Parliament and The Council of the European Union 2008). Therefore, the suggested use of surface water from TGR for drinking water purposes is a valid option. Current treatment methods, however, do not seem to be efficient since organic pollutants were detected in significant concentrations in purified tap water.

Ambient concentrations of PM and associated elemental and ionic species were measured over the cold and the warm months of 2010 at an urban and two rural sites located in the lignite-fired power generation area of Megalopolis in Peloponnese, southern Greece. The PM concentrations at the urban site (44.2 ± 33.6 μg m) were significantly higher than those at the rural sites (23.7 ± 20.4 and 22.7 ± 26.9 μg m). Source apportionment of PM and associated components was accomplished by an advanced computational procedure, the robotic chemical mass balance model (RCMB), using chemical profiles for a variety of local fugitive dust sources (power plant fly ash, flue gas desulfurization wet ash, feeding lignite, infertile material from the opencast mines, paved and unpaved road dusts, soil), which were resuspended and sampled through a PM inlet onto filters and then chemically analyzed, as well as of other common sources such as vehicular traffic, residential oil combustion, biomass burning, uncontrolled waste burning, marine aerosol, and secondary aerosol formation. Geological dusts (road/soil dust) were found to be major PM contributors in both the cold and warm periods of the year, with average annual contribution of 32.6 % at the urban site vs. 22.0 and 29.0 % at the rural sites. Secondary aerosol also appeared to be a significant source, contributing 22.1 % at the urban site in comparison to 30.6 and 28.7 % at the rural sites. At all sites, the contribution of biomass burning was most significant in winter (28.2 % at the urban site vs. 14.6 and 24.6 % at the rural sites), whereas vehicular exhaust contribution appeared to be important mostly in the summer (21.9 % at the urban site vs. 11.5 and 10.5 % at the rural sites). The highest contribution of fly ash (33.2 %) was found at the rural site located to the north of the power plants during wintertime, when winds are favorable. In the warm period, the highest contribution of fly ash was found at the rural site located to the south of the power plants, although it was less important (7.2 %). Moderate contributions of fly ash were found at the urban site (5.4 and 2.7 % in the cold and the warm period, respectively). Finally, the mine field was identified as a minor PM source, occasionally contributing with lignite dust and/or deposited wet ash dust under dry summer conditions, with the summertime contributions ranging between 3.1 and 11.0 % among the three sites. The non-parametric bootstrapped potential source contribution function analysis was further applied to localize the regions of sources apportioned by the RCMB. For the majority of sources, source regions appeared as being located within short distances from the sampling sites (within the Peloponnesse Peninsula). More distant Greek areas of the NNE sector also appeared to be source regions for traffic emissions and secondary calcium sulfate dust.

Metal mining faces a number of significant economic and environmental challenges in the twenty-first century for which established and emerging biotechnologies may, at least in part, provide the answers. Bioprocessing of mineral ores and concentrates is already used in variously engineered formats to extract base (e.g., copper, cobalt, and nickel) and precious (gold and silver) metals in mines throughout the world, though it remains a niche technology. However, current projections of an increasing future need to use low-grade primary metal ores, to reprocess mine wastes, and to develop in situ leaching technologies to extract metals from deep-buried ore bodies, all of which are economically more amenable to bioprocessing than conventional approaches (e.g., pyrometallurgy), would suggest that biomining will become more extensively utilized in the future. Recent research has also shown that bioleaching could be used to process a far wider range of metal ores (e.g., oxidized ores) than has previously been the case. Biotechnologies are also being developed to control mine-related pollution, including securing mine wastes (rocks and tailings) by using “ecological engineering” approaches, and also to remediate and recover metals from waste waters, such as acid mine drainage. This article reviews the current status of biotechnologies within the mining sector and considers how these may be developed and applied in future years.

There is extensive evidence of the negative impacts on health linked to the rise of the regional background of particulate matter (PM) 10 levels. These levels are often increased over urban areas becoming one of the main air pollution concerns. This is the case on the Bilbao metropolitan area, Spain. This study describes a data-driven model to diagnose PM10 levels in Bilbao at hourly intervals. The model is built with a training period of 7-year historical data covering different urban environments (inland, city centre and coastal sites). The explanatory variables are quantitative—log [NO₂], temperature, short-wave incoming radiation, wind speed and direction, specific humidity, hour and vehicle intensity—and qualitative—working days/weekends, season (winter/summer), the hour (from 00 to 23 UTC) and precipitation/no precipitation. Three different linear regression models are compared: simple linear regression; linear regression with interaction terms (INT); and linear regression with interaction terms following the Sawa’s Bayesian Information Criteria (INT-BIC). Each type of model is calculated selecting two different periods: the training (it consists of 6 years) and the testing dataset (it consists of 1 year). The results of each type of model show that the INT-BIC-based model (R² = 0.42) is the best. Results were R of 0.65, 0.63 and 0.60 for the city centre, inland and coastal sites, respectively, a level of confidence similar to the state-of-the art methodology. The related error calculated for longer time intervals (monthly or seasonal means) diminished significantly (R of 0.75–0.80 for monthly means and R of 0.80 to 0.98 at seasonally means) with respect to shorter periods.

Intraspecific variation in six cultivars of clover Trifolium alexandrinum L., (Bundel, Wardan, JHB-146, Saidi, Fahli, and Mescavi) has been studied with ambient and elevated O3 (ambient + 10 ppb O3) in open top chambers. Significant effect of elevated O3 was detected on different morphological, physiological, and biochemical parameters depicting differential response among the test cultivars. Results showed that the magnitude of O3 induced foliar injury symptoms varied in all the cultivars. Ozone significantly depressed photosynthetic rate, stomatal conductance, and photosynthetic efficiency, although variations were cultivar specific. Ozone treatment diminished total biomass of all the cultivars; reduction was highest in Wardan with least O3 resistance followed by Bundel, JHB-146, Saidi, Mescavi, and Fahli. According to the cumulative sensitive index, variations in the sensitivity showed that two cultivars (Wardan and Bundel) were sensitive to elevated O3, while other three cultivars (Fahli, Saidi, and Mescavi) were resistant, and JHB-146 showed intermediate sensitivity. Therefore, the present study supported the selection of sensitive cultivar of clover as a bioindicator for O3 under Indian conditions for the areas experiencing higher concentrations of O3.

Phthalic acid esters (PAEs) pollution in agricultural soils caused by widely employed plastic products is becoming more and more widespread in China. PAEs polluted soil can lead to phytotoxicity in higher plants and potential health risks to human being. We evaluated the individual toxicity of di-n-butyl phthalate (DnBP) and bis(2-ethylhexyl) phthalate (DEHP), two representative PAEs, to sown rape (Brassica chinensis L.) seeds within 72 h (as germination stage) and seedlings after germination for 14 days by monitoring responses and trends of different biological parameters. No significant effects of six concentrations of PAE ranging from 0 (not treated/NT) to 500 mg kg(-1) on germination rate in soil were observed. However, root length, shoot length, and biomass (fresh weight) were inhibited by both pollutants (except root length and biomass under DEHP). Stimulatory effects of both target pollutants on malondialdehyde (MDA) content, superoxide dismutase (SODase) activity, ascorbate peroxidase (APXase) content, and polyphenoloxidase (PPOase) activity in shoots and roots (SODase activity in shoots excluded) were in the same trend with the promotion of proline (Pro) but differed with acetylcholinesterase activity (except in shoots under DnBP) for analyzed samples treated for 72 h and 14 days. Responses of representative storage compounds free amino acids (FAA) and total soluble sugar (TSS) under both PAEs were raised. Sensitivity of APXase and Pro in roots demonstrates their possibility in estimation of PAE phytotoxicity and the higher toxicity of DnBP, which has also been approved by the morphological photos of seedlings at day 14. Higher sensitivity of the roots was also observed. The recommended soil allowable concentration is 5 mg DnBP kg(-1) soil for the development of rape. We still need to know the phytotoxicity of DEHP at whole seedling stage for both the growing and development; on the other hand, soil criteria for PAE compounds are urgently required in China.

Extracellular polymeric substances (EPS) are, along with microbial cells, the main components of the biological sludges used in wastewater treatment and natural biofilms. EPS play a major role in removing pollutants from water by means of sorption. The ability of soluble EPS (S-EPS) and bound EPS (B-EPS) derived from various bacterial aggregates (flocs, granules, biofilms) to bind at pH 7.0 ± 0.1 to two pharmaceutical substances, acetaminophen (ACE) and erythromycin ethylsuccinate (ERY), has been investigated using the fluorescence quenching method. Two intense fluorescence peaks, A (Ex/Em range, 200-250/275-380 nm) and B (Ex/Em range, 260-320/275-360 nm), corresponding respectively to the aromatic protein region and soluble microbial by-product-like region, were identified in a three-dimensional excitation-emission matrix of EPS samples. The fluorescence peak, which corresponds to humic-like substances, was also identified though at low intensity. The ability of EPS to bind ACE was found to exceed that for ERY. The aromatic protein fraction of EPS displays a slightly higher affinity for drugs than that shown by the soluble microbial by-product-like fraction. The S-EPS and B-EPS present the same affinity for ACE and ERY. The effective quenching constants (log K) derived from the Stern-Volmer Equation equaled at peak A (with S-EPS): 3.7 ± 0.2 to 4.0 ± 0.1 for ACE and 2.1 ± 0.3 to 2.7 ± 0.1 for ERY. With B-EPS, these values were 3.9 ± 0.1 to 4.0 ± 0.1 for ACE and 2.0 ± 0.2 to 2.6 ± 0.1 for ERY. Our results suggest that the weaker EPS affinity for ERY than for ACE serves to partially explain why only about 50-80 % of ERY is removed from wastewater at the treatment plant. Moreover, this work demonstrates that EPS from natural river biofilms are able to bind drugs, which in turn may limit the mobility of drugs in natural waters.

Ciliated protozoa (ciliates) play important ecological roles in coastal waters, especially regarding their interaction with environmental parameters. In order to increase our knowledge and understanding on the functional structure of ciliate communities and their relationships to environmental conditions in marine ecosystems, a 12-month study was carried out in a semi-enclosed bay in northern China. Samples were collected biweekly at five sampling stations with differing levels of pollution/eutrophication, giving a total of 120 samples. Thirteen functional groups of ciliates (A–M) were defined based on their specific spatio-temporal distribution and relationships to physico-chemical parameters. Six of these groups (H–M) were the primary contributors to the ciliate communities in the polluted/eutrophic areas, whereas the other seven groups (A–G) dominated the communities in less polluted areas. Six groups (A, D, G, H, I and K) dominated during the warm seasons (summer and autumn), with the other seven (B, C, E, F, J, L and M) dominating in the cold seasons (spring and winter). Of these, groups B (mainly aloricate ciliates), I (aloricate ciliates) and L (mainly loricate tintinnids) were the primary contributors to the communities. It was also shown that aloricate ciliates and tintinnids represented different roles in structuring and functioning of the communities. The results suggest that the ciliate communities may be constructed by several functional groups in response to the environmental conditions. Thus, we conclude that these functional groups might be potentially useful bioindicators for bioassessment and conservation in marine habitats.

In the last decade, various molecular methods (e.g., fluorescent hybridization assay, sandwich hybridization assay, automatized biosensor detection, real-time PCR assay) have been developed and implemented for accurate and specific identification and estimation of marine toxic microalgal species. This review focuses on the recent quantitative real-time PCR (qrt-PCR) technology developed for the control and monitoring of the most important taxonomic phytoplankton groups producing biotoxins with relevant negative impact on human health, the marine environment, and related economic activities. The high specificity and sensitivity of the qrt-PCR methods determined by the adequate choice of the genomic target gene, nucleic acid purification protocol, quantification through the standard curve, and type of chemical detection method make them highly efficient and therefore applicable to harmful algal bloom phenomena. Recent development of qrt-PCR-based assays using the target gene of toxins, such as saxitoxin compounds, has allowed more precise quantification of toxigenic species (i.e., Alexandrium catenella) abundance. These studies focus only on toxin-producing species in the marine environment. Therefore, qrt-PCR technology seems to offer the advantages of understanding the ecology of harmful algal bloom species and facilitating the management of their outbreaks.

The impact of the insecticide chlorpyrifos (CPF) on the mammalian digestive system has been poorly described. The present study aimed at evaluating the effect of chronic, low-dose exposure to CPF on the composition of the gut microbiota in a Simulator of the Human Intestinal Microbial Ecosystem: the SHIME® and in rats. The SHIME® comprises six reactor vessels (stomach to colon). The colonic segments were inoculated with feces from healthy humans. Then, the simulator was exposed to a daily dose of 1 mg of CPF for 30 days. The changes over time in the populations of bacteria were examined at different time points: prior to pesticide exposure (as a control) and after exposure. In parallel, pregnant rats were gavaged daily with 1 mg/kg of CPF (or vehicle) until the pups were weaned. Next, the rats were gavaged with same dose of CPF until 60 days of age (adulthood). Then, samples of different parts of the digestive tract were collected under sterile conditions for microbiological assessment. Chronic, low-dose exposure to CPF in the SHIME® and in the rat was found to induce dysbiosis in the microbial community with, in particular, proliferation of subpopulations of some strains and a decrease in the numbers of others bacteria. In compliance with European guidelines, the use of the SHIME® in vitro tool would help to (1) elucidate the final health effect of toxic agents and (2) minimize (though not fully replace) animal testing. Indeed, certain parameters would still have to be studied further in vivo.