The Southern California coastal sewage outfalls were investigated in relation to their effect upon standing stocks of phytoplankton, and on primary production. Kinetic parameters for the assimilation of ammonium, nitrate, and urea were determined at the outfall sites using N-15 labeled substrates. Laboratory studies investigated the utilization of various forms of nitrogen by phytoplankton, mechanisms and rates of nitrogen assimilation, and enzymes of nitrogen assimilation. It was found that the maximum growth rate was a variable, while the saturation constant was uniform over a range of dilution rates of N-limited chemostat cultures. The chemical composition of phytoplankton varied with dilution rate in reproducible ways. By varying the dilution rate of such cultures one seems to regulate the degree of nitrogen-deficiency of the phytoplankton.

Effects of rainfall runoff from a beef cattle feedlot on the water quality of a small impoundment were determined. Changes in chemical concentration of impounded water and changes in the community structure of aquatic organisms were measured and related to the amount and composition of feedlot runoff received. Water quality changes were also monitored in a nearby reservoir which received no feedlot runoff to serve as a control. Changes in chemical concentration or population structure of organisms were not apparent for discharges of about one-part feedlot runoff to 40 parts receiving water. Runoff discharges for two pumping periods with each contributing one-fourth of the volume of the receiving water were shown to degrade water quality in the impoundment. Several significant chemical and biological changes occurred. The most dramatic reduction in the biological community was the suffocation of about 90% of the game fish in the impoundment.

A project has been underway which attempts to demonstrate practical means of abating pollution from coal mine mineral wastes. The site included a refuse pile occupying approximately 40 acres and a slurry lagoon complex of 50 acres. In the report, the characteristics and acid formation rate of the refuse pile are described. The average rate of acid formation for this refuse pile is 198 pounds of acidity, as CaCO₃, per acre per day. Acid contribution from the slurry lagoons was not determined but appears to be negligible. As an abatement measure, a number of experimental vegetative covers were tested. Grass was successfully established with and without the use of topsoil, weathering well for one year. The long-term effects of establishing a grass cover directly on the refuse without the use of topsoil are not known at this time.

Instantaneous amounts of organic carbon, both particulate and dissolved, in a number of freshwaters ranging from unproductive tarns to moderately productive reservoirs were measured by acid-persulfate digestion and infrared absorption. Organic carbon was designated as net seston carbon, filter seston carbon, or dissolved carbon based on filtering techniques. Repeated sampling showed amounts of organic carbon, either dissolved or particulate, fluctuated considerably over short periods of time; the dissolved fraction probably varying less than particulate forms. Almost daily measurements in samples incubated over three week periods also revealed erratic changes, with no clear reduction in total organic carbon. The data have descriptive value, although they cannot, at this time, be related satisfactorily to the general limnological knowledge.

The water impounded at Indian Creek Reservoir near Lake Tahoe, California is approximately one-third surface runoff and direct precipitation and two-thirds reclaimed water exported from South Tahoe Public Utility District Plant. Discussed are the results of tests which determined the temporal changes and relationships between water quality characteristics of Indian Creek Reservoir and those of the reclaimed water. Initially the reservoir would not support fish life, but as the reservoir matured, ammonia levels declined to less than 4 mg/l and by 1970 it was an excellent trout fishery. Approximately 70% of the ammonia nitrogen was lost to the atmosphere by nitrification-denitrification. Good biological productivity indicated access to other phosphorus sources, probably runoff. Relative to conductivity and chemical components the water is of good irrigation quality. Various parameters showed that the reservoir responds to more complex factors than those measurable in the reclaimed waste water, raising the question of the effectiveness of tertiary treatment for recreational impoundments.