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Blood mercury concentrations in four sympatric gull species from South Western France: Insights from stable isotopes and biologging Full text
2022
Jouanneau, William | Sebastiano, Manrico | Rozen-Rechels, David | Harris, Stephanie | Blévin, Pierre | Angelier, Frédéric | Brischoux, François | Gernigon, Julien | Lemesle, Jean-Christophe | Robin, Frédéric | Cherel, Yves | Bustamante, Paco | Chastel, Olivier | Centre d'Études Biologiques de Chizé - UMR 7372 (CEBC) ; La Rochelle Université (ULR)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Physiologie moléculaire et adaptation (PhyMA) ; Muséum national d'Histoire naturelle (MNHN)-Centre National de la Recherche Scientifique (CNRS) | Institut d'écologie et des sciences de l'environnement de Paris (iEES Paris) ; Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | College of Environmental Science and Engineering ; School of Ocean Sciences | Ligue pour la Protection des Oiseaux (LPO) | LIttoral ENvironnement et Sociétés (LIENSs) ; Institut national des sciences de l'Univers (INSU - CNRS)-La Rochelle Université (ULR)-Centre National de la Recherche Scientifique (CNRS) | Institut universitaire de France (IUF) ; Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.)
Blood mercury concentrations in four sympatric gull species from South Western France: Insights from stable isotopes and biologging Full text
2022
Jouanneau, William | Sebastiano, Manrico | Rozen-Rechels, David | Harris, Stephanie | Blévin, Pierre | Angelier, Frédéric | Brischoux, François | Gernigon, Julien | Lemesle, Jean-Christophe | Robin, Frédéric | Cherel, Yves | Bustamante, Paco | Chastel, Olivier | Centre d'Études Biologiques de Chizé - UMR 7372 (CEBC) ; La Rochelle Université (ULR)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Physiologie moléculaire et adaptation (PhyMA) ; Muséum national d'Histoire naturelle (MNHN)-Centre National de la Recherche Scientifique (CNRS) | Institut d'écologie et des sciences de l'environnement de Paris (iEES Paris) ; Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | College of Environmental Science and Engineering ; School of Ocean Sciences | Ligue pour la Protection des Oiseaux (LPO) | LIttoral ENvironnement et Sociétés (LIENSs) ; Institut national des sciences de l'Univers (INSU - CNRS)-La Rochelle Université (ULR)-Centre National de la Recherche Scientifique (CNRS) | Institut universitaire de France (IUF) ; Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.)
International audience | Mercury (Hg) is a toxic trace element widely distributed in the environment, which particularly accumulates in top predators, including seabirds. Among seabirds, large gulls (Larus sp) are generalist feeders, foraging in both terrestrial and marine habitats, making them relevant bioindicators of local coastal Hg contamination. In the present study, we reported blood Hg concentrations in adults and chicks of four different gull species breeding on the French Atlantic coast: the European herring gull (Larus argentatus), the Lesser black-backed gull (L. fuscus), the Great black-backed gull (L. marinus) and the Yellow-legged gull (L. michahellis). We also investigated the potential role of foraging ecology in shaping Hg contamination across species, using the unique combination of three dietary tracers (carbon, nitrogen and sulfur stable isotopes) and biologging (GPS tracking). A high concentration of Hg was associated with high trophic position and a marine diet in gulls, which was corroborated by birds’ space use strategy during foraging trips. Adults of all four species reached Hg concentrations above reported toxicity thresholds. Specifically, adults of Great black-backed gulls had a high trophic marine specialized diet and significantly higher Hg concentrations than the three other species. Blood Hg was 4–7 times higher in adults than in chicks, although chicks of all species received mainly marine and high trophic position prey, which is expected to be the cause of blood Hg concentrations of toxic concern. By using both stable isotopes and GPS tracking, the present study provides compelling insights on the main feeding habits driving Hg contamination in a seabird assemblage feeding in complex coastal environments.
Show more [+] Less [-]Blood mercury concentrations in four sympatric gull species from South Western France: Insights from stable isotopes and biologging Full text
2022
Jouanneau, William | Sebastiano, Manrico | Rozen-Rechels, David | Harris, Stephanie | Blévin, Pierre | Angelier, Frédéric | Brischoux, François | Gernigon, Julien | Lemesle, Jean-Christophe | Robin, Frédéric | Cherel, Yves | Bustamante, Paco | Chastel, Olivier
International audience | Mercury (Hg) is a toxic trace element widely distributed in the environment, which particularly accumulates in top predators, including seabirds. Among seabirds, large gulls (Larus sp) are generalist feeders, foraging in both terrestrial and marine habitats, making them relevant bioindicators of local coastal Hg contamination. In the present study, we reported blood Hg concentrations in adults and chicks of four different gull species breeding on the French Atlantic coast: the European herring gull (Larus argentatus), the Lesser black-backed gull (L. fuscus), the Great black-backed gull (L. marinus) and the Yellow-legged gull (L. michahellis). We also investigated the potential role of foraging ecology in shaping Hg contamination across species, using the unique combination of three dietary tracers (carbon, nitrogen and sulfur stable isotopes) and biologging (GPS tracking). A high concentration of Hg was associated with high trophic position and a marine diet in gulls, which was corroborated by birds’ space use strategy during foraging trips. Adults of all four species reached Hg concentrations above reported toxicity thresholds. Specifically, adults of Great black-backed gulls had a high trophic marine specialized diet and significantly higher Hg concentrations than the three other species. Blood Hg was 4–7 times higher in adults than in chicks, although chicks of all species received mainly marine and high trophic position prey, which is expected to be the cause of blood Hg concentrations of toxic concern. By using both stable isotopes and GPS tracking, the present study provides compelling insights on the main feeding habits driving Hg contamination in a seabird assemblage feeding in complex coastal environments.
Show more [+] Less [-]Blood mercury concentrations in four sympatric gull species from South Western France: Insights from stable isotopes and biologging Full text
2022
Jouanneau, William | Sebastiano, Manrico | Rozen-Rechels, David | Harris, Stephanie M. | Blévin, Pierre | Angelier, Frédéric | Brischoux, François | Gernigon, Julien | Lemesle, Jean-Christophe | Robin, Frédéric | Cherel, Yves | Bustamante, Paco | Chastel, Olivier
Mercury (Hg) is a toxic trace element widely distributed in the environment, which particularly accumulates in top predators, including seabirds. Among seabirds, large gulls (Larus sp) are generalist feeders, foraging in both terrestrial and marine habitats, making them relevant bioindicators of local coastal Hg contamination. In the present study, we reported blood Hg concentrations in adults and chicks of four different gull species breeding on the French Atlantic coast: the European herring gull (Larus argentatus), the Lesser black-backed gull (L. fuscus), the Great black-backed gull (L. marinus) and the Yellow-legged gull (L. michahellis). We also investigated the potential role of foraging ecology in shaping Hg contamination across species, using the unique combination of three dietary tracers (carbon, nitrogen and sulfur stable isotopes) and biologging (GPS tracking). A high concentration of Hg was associated with high trophic position and a marine diet in gulls, which was corroborated by birds’ space use strategy during foraging trips. Adults of all four species reached Hg concentrations above reported toxicity thresholds. Specifically, adults of Great black-backed gulls had a high trophic marine specialized diet and significantly higher Hg concentrations than the three other species. Blood Hg was 4–7 times higher in adults than in chicks, although chicks of all species received mainly marine and high trophic position prey, which is expected to be the cause of blood Hg concentrations of toxic concern. By using both stable isotopes and GPS tracking, the present study provides compelling insights on the main feeding habits driving Hg contamination in a seabird assemblage feeding in complex coastal environments.
Show more [+] Less [-]New insights on glass industry wasteland ecosystems Full text
2022
Jacquet, Julien | Benizri, Emile | Echevarria, Guillaume | Sirguey, Catherine | ECONICK, 1 rue Granville, 54000 Nancy, France | Laboratoire Sols et Environnement (LSE) ; Université de Lorraine (UL)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | ANRT convention Cifre n ◦ 2019/1926 | ADEME convention n◦ 1972C0020 | Projet SOLAMET | GISFI research consortium
International audience | Glass manufacturing operations lead to an increasing number of abandoned slag heaps contaminated with metallic trace elements (MTE). However, the relative influence of edaphic factors on the biodiversity of glasswork wastelands is still poorly understood although closely related to sustainable land management practices. Therefore, the objectives of this research were to provide new insights into glasswork wastelands through the investigation of (i) Orthoptera, diurnal Lepidoptera, plant communities, and (ii) abiotic parameters in the topsoils. To that end, biodiversity indices were computed from ecological inventories performed on the herbaceous layer. In addition, soil samples were taken from the topsoil layer (0-10 cm) to assess agronomic properties, actually (CEC-exchangeable) and potentially bioavailable MTE fractions (DTPA-extractable) and pseudo-total MTE contents. On the one hand, the studied site was able to support a substantially higher than excepted biodiversity with orthopteran assemblages similar to grasslands and a diurnal Lepidoptera diversity comparable to urban parks. We also noted a positive influence of plant richness on the diurnal Lepidoptera community structure. On the other hand, topsoil analysis revealed a severe Pb contamination (1800-3100 mg kg − 1) and a high potentially bioavailable Pb fraction (800-1300 mg kg − 1). However, CEC-exchangeable MTE concentrations were all below the analytical quantification limits. Moreover, the site was characterized by a medium soil fertility. From these results, Pb contamination does not appear to be a primary limiting factor for the establishment of these communities. We assume that glasswork wasteland ecosytems are more affected by soil fertility or land management practices. To conclude, these sites are able to provide biodiversity ecosystem services, acting as wildlife sanctuaries for Orthoptera and diurnal Lepidoptera, and strategic metals by phytoextraction in a circular economy model. Thus, wasteland management practices should consider the local-scale drivers of biodiversity in order to reach at least the zero net loss of biodiversity. ☆ This paper has been recommended for acceptance by Amit Bhatnagar.
Show more [+] Less [-]Morocco's coastal aquifers: Recent observations, evolution and perspectives towards sustainability Full text
2022
Ez-Zaouy, Yassine | Bouchaou, Lhoussaine | Saad, Aicha | Hssaisoune, Mohammed | Brouziyne, Youssef | Dhiba, Driss | Chehbouni, Abdelghani | Université Mohammed VI Polytechnique = Mohammed VI Polytechnic University [Ben Guerir] (UM6P) | Université Ibn Zohr = Ibn Zohr University [Agadir] | Centre d'études spatiales de la biosphère (CESBIO) ; Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National d'Études Spatiales [Toulouse] (CNES)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
Morocco's coastal aquifers: Recent observations, evolution and perspectives towards sustainability Full text
2022
Ez-Zaouy, Yassine | Bouchaou, Lhoussaine | Saad, Aicha | Hssaisoune, Mohammed | Brouziyne, Youssef | Dhiba, Driss | Chehbouni, Abdelghani | Université Mohammed VI Polytechnique = Mohammed VI Polytechnic University [Ben Guerir] (UM6P) | Université Ibn Zohr = Ibn Zohr University [Agadir] | Centre d'études spatiales de la biosphère (CESBIO) ; Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3) ; Université de Toulouse (UT)-Université de Toulouse (UT)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National d'Études Spatiales [Toulouse] (CNES)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
International audience | During the last decades, the coastal areas of Morocco have witnessed an intense socioeconomic development associated with a continuous population growth and urban extension. This has led to an overexploitation of coastal aquifers leading to a degradation of their water quality. In order to obtain large scale overview on the quality status of Morocco's coastal aquifers (MCA) to assist national water managers to make informed decisions, a comprehensive scrutinization of the MCA against common indicators and using unified methods is essential. In this study, databases from thirteen MCA were analyzed, using multivariate statistical approaches and graphical methods in order to investigate the degree of mineralization in each aquifer and to identify the main salinization processes prevailing in groundwater. The results showed that the dominant groundwater types are Na–Cl, Ca–Mg–Cl, Ca–Mg–SO4, Ca–Mg–HCO3 and Ca–HCO3–Cl. The Gibbs diagram and the seawater contribution (0–37%) indicate that the mineralization is mainly due to the seawater intrusion and water-rock interaction. The salinity degree diagram illustrates that almost all groundwater samples are located in the moderate to very saline zone, indicating that MCA are recharged by water from variable sources. The groundwater quality assessment shows a deterioration, particularly by seawater intrusion and significant nitrate pollution. The temporal evolution confirm that the MCA are influenced by seawater namely in the Atlantic part. The Wilcox and USSL diagram indicate that the majority of sampled water are unsuitable for irrigation uses. In addition, and by referring to the WHO and the Moroccan standards for water potability, large number of samples from the groundwaters of the MCA is not fully adequate for drinking purposes. A set of management actions (e,g., artificial recharge) are proposed in order to mitigate the effect of groundwater overexploitation and seawater intrusion to ensure the sustainability of MCA.
Show more [+] Less [-]Morocco's coastal aquifers: Recent observations, evolution and perspectives towards sustainability Full text
2022
Ez-zaouy, Yassine | Bouchaou, Lhoussaine | Saad, Aicha | Hssaisoune, Mohammed | Brouziyne, Youssef | Dhiba, Driss | Chehbouni, Abdelghani
During the last decades, the coastal areas of Morocco have witnessed an intense socioeconomic development associated with a continuous population growth and urban extension. This has led to an overexploitation of coastal aquifers leading to a degradation of their water quality. In order to obtain large scale overview on the quality status of Morocco's coastal aquifers (MCA) to assist national water managers to make informed decisions, a comprehensive scrutinization of the MCA against common indicators and using unified methods is essential. In this study, databases from thirteen MCA were analyzed, using multivariate statistical approaches and graphical methods in order to investigate the degree of mineralization in each aquifer and to identify the main salinization processes prevailing in groundwater. The results showed that the dominant groundwater types are Na–Cl, Ca–Mg–Cl, Ca–Mg–SO₄, Ca–Mg–HCO₃ and Ca–HCO₃–Cl. The Gibbs diagram and the seawater contribution (0–37%) indicate that the mineralization is mainly due to the seawater intrusion and water-rock interaction. The salinity degree diagram illustrates that almost all groundwater samples are located in the moderate to very saline zone, indicating that MCA are recharged by water from variable sources. The groundwater quality assessment shows a deterioration, particularly by seawater intrusion and significant nitrate pollution. The temporal evolution confirm that the MCA are influenced by seawater namely in the Atlantic part. The Wilcox and USSL diagram indicate that the majority of sampled water are unsuitable for irrigation uses. In addition, and by referring to the WHO and the Moroccan standards for water potability, large number of samples from the groundwaters of the MCA is not fully adequate for drinking purposes. A set of management actions (e,g., artificial recharge) are proposed in order to mitigate the effect of groundwater overexploitation and seawater intrusion to ensure the sustainability of MCA.
Show more [+] Less [-]Coastal gradients of small microplastics and associated pollutants influenced by estuarine sources Full text
2022
González-Ortegón, Enrique | Sendra, Marta | Sparaventi, Erica | Sanchez Leal, Ricardo F. | Ríos Hierro, Isaac de los | Baldó, Francisco | González Fernández, Daniel | Yeste Siguenza, María del Pilar | Biología | Ciencia de los Materiales e Ingeniería Metalúrgica y Química Inorgánica
Small microplastics (SMPs) in the gulf of Cadiz was sampled at 5 m depth by pumping it through the ship's pipe system and filtered through a 45 mu m mesh size net. Our study reveals that higher densities have been found (130 mg.m(-3)) compared to other regions worldwide and these densities decreased from the coastline to the outer stations, showing a general coastal gradient influenced by estuarine outflows. SMPs with a size range between 45 and 193 mu m were predominant and most of them composed by polyethylene and polypropylene. The metals associated with the MPs were mainly Na (21.1%), K (11.3%), Fe (8.5%), Ca (2.1%), Cr (1.8%), Zr (13.3%) and Hf (0.7%). The high proportion of Zr compared to Fe, which is different from what can be found in the environment, | Financial support to EGO and the instrument was given by CSIC through Intramural Research program 2018 under grant number 201830I081. MS is grateful to the Spanish Ministry of Science, Innovation and Universities for the Juan de la Cierva (TJFI-2017-32493) contract. DGF was funded by European Union's H2020-MSCA-IF-2018 846843-LitRivus.
Show more [+] Less [-]Assessment of elemental composition in commercial fish of the Bay of Cádiz, Southern Iberian Peninsula Full text
2022
Calero Cano, Sandra | Donázar Aramendía, Íñigo | Morales, Emilio | Arechavala Lopez, Pablo | Cervera Currado, Juan Lucas | Guerra-Garcia, Jose Manuel | Giráldez, Inmaculada | Biología
The assessment of trace metal content in our fish diet is important due to the adverse effect on human health. Despite the increasing interest about the fish quality, little information is available for Southern Spain, a region characterized by high seafood intake. Nine species from the Bay of Cádiz with high commercial value were selected. Similar values were measured in the nine studied species for most of the elements, except for the macroelements Ca and S, and the microelements Fe, Mn and As, which showed significant differences among species. Metal Pollution Index (MPI) did not differ among species, and it was similar to those obtained for other Atlantic and Mediterranean locations. The values measured for the nine species were below the health limits provided by World, European and Spanish legislations, indicating that, in general terms, consumption of these species is safe in the study area. | 9 páginas
Show more [+] Less [-]Microplastics concentrations in soil along a racetrack | Microplastics concentrations in soil along a racetrack Full text
2022
Mengistu, Demmelash | Nilsen, Vegard | Coutris, Claire | Amdal, Helena Marie | Heistad, Arve
Microplastics concentrations in soil along a racetrack | Microplastics concentrations in soil along a racetrack Full text
2022
Mengistu, Demmelash | Nilsen, Vegard | Coutris, Claire | Amdal, Helena Marie | Heistad, Arve
Motorsport is known for its high tire wear due to speed, cornering, and high acceleration/deceleration activities. However, studies on the generation of microplastics from racetracks are rare. This study aimed at quantifying microplastics concentrations in topsoil (0–5 cm) along a racetrack. The results showed that rubber materials (RM) and tire reinforcement microplastics (TRMP) were deposited in the soil along the racetrack. Concentrations of the two microplastics were affected by the distance from the edge of the racetrack (highest concentrations within 20 cm from the track) and track alignment (highest concentrations at the start/finish area). In addition, a weak correlation was observed between the concentrations of the two microplastics, suggesting the effect of track alignment on the type of microplastics abraded. The results also showed that coarser microplastics (1000–5000 μm) dominate the size distribution of microplastics along a racetrack. The findings of this study may provide racetrack managers with basic information for designing microplastic-controlling solutions. While additional studies are required to map environmental effects and policy measures, our initial results suggest that motorsport is of concern in terms of microplastics release to the environment. | Microplastics concentrations in soil along a racetrack | publishedVersion
Show more [+] Less [-]Microplastics concentrations in soil along a racetrack Full text
2022
Motorsport is known for its high tire wear due to speed, cornering, and high acceleration/deceleration activities. However, studies on the generation of microplastics from racetracks are rare. This study aimed at quantifying microplastics concentrations in topsoil (0–5 cm) along a racetrack. The results showed that rubber materials (RM) and tire reinforcement microplastics (TRMP) were deposited in the soil along the racetrack. Concentrations of the two microplastics were affected by the distance from the edge of the racetrack (highest concentrations within 20 cm from the track) and track alignment (highest concentrations at the start/finish area). In addition, a weak correlation was observed between the concentrations of the two microplastics, suggesting the effect of track alignment on the type of microplastics abraded. The results also showed that coarser microplastics (1000–5000 μm) dominate the size distribution of microplastics along a racetrack. The findings of this study may provide racetrack managers with basic information for designing microplastic-controlling solutions. While additional studies are required to map environmental effects and policy measures, our initial results suggest that motorsport is of concern in terms of microplastics release to the environment. | Microplastics concentrations in soil along a racetrack
Show more [+] Less [-]Sublethal effects of metal toxicity and the measure of plant fitness in ecotoxicological experiments Full text
2022
Nowak, Julien | Faure, Nathalie | Glorieux, Cédric | Vile, Denis | Pauwels, Maxime | Frérot, Hélène | Évolution, Écologie et Paléontologie (Evo-Eco-Paleo) - UMR 8198 (Evo-Eco-Paléo (EEP)) ; Université de Lille-Centre National de la Recherche Scientifique (CNRS) | Écophysiologie des Plantes sous Stress environnementaux (LEPSE) ; Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Montpellier ; Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro) | Laboratoire Avancé de Spectroscopie pour les Intéractions la Réactivité et l'Environnement - UMR 8516 (LASIRE) ; Institut de Chimie - CNRS Chimie (INC-CNRS)-Université de Lille-Centre National de la Recherche Scientifique (CNRS) | French Ministry of Higher Education, Research and Innovation. | ANR-12-JSV7-0010,ELOCANTH,Evolution de l'adaptation locale en environnement anthropisé(2012)
International audience | Anthropogenic pollution is a major driver of global environmental change. To be properly addressed, the study of the impact of pollutants must consider both lethal effects and sublethal effects on individual fitness. However, measuring fitness remains challenging. In plants, the total number of seeds produced, i.e. the seed set, is traditionally considered, but is not readily accessible. Instead, performance traits related to survival, e.g., vegetative biomass and reproductive success, can be measured, but their correlation with seed set has rarely been investigated. To develop accurate estimates of seed set, relationships among 15 vegetative and reproductive traits were analyzed. For this purpose, Noccaea caerulescens (Brassicaceae), a model plant to study local adaptation to metalcontaminated environments, was used. To investigate putative variation in trait relationships, sampling included several accessions cultivated in contrasting experimental conditions. To test their applicability, selected estimates were used in the first generation of a Laboratory Natural Selection (LNS) experiment exposing experimentally plants to zinc soil pollution. Principal component analyses revealed statistical independence between vegetative and reproductive traits. Traits showing the strongest positive correlation with seed set were the number of non-aborted silicles, and the product of this number and mean silicle length. They thus appeared the most appropriate to document sublethal or fitness effects of environmental contaminants in plant ecotoxicological studies. The relevance of both estimates was confirmed by using them to assess the fitness of parental plants of the first generation of an LNS experiment: the same families consistently displayed the highest or the lowest performance values in two independent experimental metal-exposed populations. Thus, both these fitness estimates could be used to determine the expected number of offspring and the composition of successive generations in further LNS experiments investigating the impact of multi-generational exposure of a plant species to environmental pollution.
Show more [+] Less [-]Multiple reaction monitoring mass spectrometry for the discovery of environmentally modulated proteins in an aquatic invertebrate sentinel species, Gammarus fossarum Full text
2022
Leprêtre, Maxime | Geffard, Olivier | Espeyte, Anabelle | Faugere, Julien | Ayciriex, Sophie | Chaumot, Arnaud | Delorme, Nicolas | Salvador, Arnaud | Degli-Esposti, Davide | RiverLy - Fonctionnement des hydrosystèmes (RiverLy) ; Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | ISA-ANABIO-MS - Analyse biomoléculaire par spectrométrie de masse - Biological Analysis by Mass Spectrometry ; Institut des Sciences Analytiques (ISA) ; Université Claude Bernard Lyon 1 (UCBL) ; Université de Lyon-Université de Lyon-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL) ; Université de Lyon-Université de Lyon-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS) | ANR-18-CE34-0013,APPROve,Démarche intégrée pour proposer la protéomique dans la surveillance : accumulation, devenir et multimarqueurs(2018)
International audience | Multiple reaction monitoring (MRM) mass spectrometry is emerging as a relevant tool for measuring customized molecular markers in freshwater sentinel species. While this technique is typically used for the validation of protein molecular markers preselected from shotgun experiments, recent gains of MRM multiplexing capacity offer new possibilities to conduct large-scale screening of animal proteomes. By combining the strength of active biomonitoring strategies and MRM technologies, this study aims to propose a new strategy for the discovery of candidate proteins that respond to environmental variability. For this purpose, 249 peptides derived from 147 proteins were monitored by MRM in 273 male gammarids caged in 56 environmental sites, representative of the diversity of French water bodies. A methodology is here proposed to identify a set of customized housekeeping peptides (HKPs) used to correct analytical batch effects and allow proper comparison of peptide levels in gammarids. A comparative analysis performed on HKPs-normalized data resulted in the identification of peptides highly modulated in the environment and derived from proteins likely involved in the environmental stress response. Overall, this study proposes a breakthrough approach to screen and identify potential proteins responding to relevant environmental conditions in sentinel species.
Show more [+] Less [-]Organic waste-borne ZnS nanoparticles: The forgotten ones Full text
2022
Levard, C. | Le Bars, M. | Formentini, T. | Legros, S. | Doelsch, E. | Centre Européen de Recherche et d'Enseignement des Géosciences de l'Environnement (CEREGE) ; Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Collège de France (CdF (institution))-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Swedish University of Agricultural Sciences = Sveriges lantbruksuniversitet (SLU) | Recyclage et risque (UPR Recyclage et risque) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad) | Département Performances des systèmes de production et de transformation tropicaux (Cirad-PERSYST) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad) | ANR-15-CE34-0003,DIGESTATE,Diagnostic des traitements des déchets et comportement des contaminants dans l'environnement(2015)
International audience
Show more [+] Less [-]Associations between internal concentrations of fluorinated and organochlorinated chemicals in women and in vitro fertilization outcomes: A multi-pollutant study Full text
2022
Lefebvre, Tiphaine | Fréour, Thomas | Duval, Gauthier | Ploteau, Stéphane | Marchand, Philippe | Le Bizec, Bruno | Antignac, Jean-Philippe | Cano-Sancho, Germán | Centre Hospitalier Universitaire de Nantes = Nantes University Hospital (CHU Nantes) | Laboratoire d'étude des Résidus et Contaminants dans les Aliments (LABERCA) ; École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) | Team 2 : Cell and gene engineering in tolerance, fertility and regenerative medicine (Team 2 - U1064 Inserm - CR2TI) ; Centre de Recherche en Transplantation et Immunologie - Center for Research in Transplantation and Translational Immunology (U1064 Inserm - CR2TI) ; Institut National de la Santé et de la Recherche Médicale (INSERM)-Nantes Université - UFR de Médecine et des Techniques Médicales (Nantes Univ - UFR MEDECINE) ; Nantes Université - pôle Santé ; Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ)-Nantes Université - pôle Santé ; Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Nantes Université - UFR de Médecine et des Techniques Médicales (Nantes Univ - UFR MEDECINE) ; Nantes Université - pôle Santé ; Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ)-Nantes Université - pôle Santé ; Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ) | Centre de Recherche en Transplantation et Immunologie - Center for Research in Transplantation and Translational Immunology (U1064 Inserm - CR2TI) ; Institut National de la Santé et de la Recherche Médicale (INSERM)-Nantes Université - UFR de Médecine et des Techniques Médicales (Nantes Univ - UFR MEDECINE) ; Nantes Université - pôle Santé ; Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ)-Nantes Université - pôle Santé ; Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ) | Nantes Université (Nantes Univ)
International audience | The impact of persistent organic pollutants (POPs) on reproductive health is still poorly understood, even though infertility management has high associated societal and economical costs. The aims of this study were to characterize the internal levels of polychlorinated biphenyls (PCBs), organochlorine pesticides (OCPs) and perfluoroalkylated substances (PFAS) in women undergoing in vitro fertilization (IVF); and evaluate their association with IVF outcomes, individually and as mixtures in a combined multipollutant approach. Thus, 136 women undergoing IVF treatment at Nantes University Hospital (France) were prospectively recruited between 2019 and 2020. Serum samples were analyzed using liquid chromatography with tandem-mass spectrometry for 14 PFAS. Follicular fluid was analyzed with gas chromatography coupled to high resolution mass spectrometry for 14 PCBs and 25 OCPs. Intermediate and clinical IVF outcomes were ascertained by embryologists and clinicians using standardized protocols. Multivariate Poisson regression models and Bayesian Kernel Machine Regressions (BKMR) were used to identify individual and joint associations between POPs and IVF outcomes adjusting for age, body mass index (BMI) and anti-Müllerian hormone. The results showed that most POPs were widely present in women, and globally not associated with clinically relevant IVF outcomes, like live birth rates. Nonetheless, negative associations between PCB138 and trans-nonachlor with useable blastocysts were identified, β -0.28 (95%CI [-0.52; -0.04] p = 0.02) and β -0.22 (95%CI [-0.40; -0.03] p = 0.02). Conversely, PCB28 showed positive associations with the number of useable blastocysts, pregnancy rate and live birth rate. The BKMR analysis suggested the lack of association of the mixture with intermediate and clinical outcomes. The study supports the need of conducting further studies in a larger population sample in order to ensure sufficient statistical power to identify modest effects and a robust stratification analysis to account for the large underlying disease heterogeneity.
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