International audience | The environmental presence of mercury has dramatically increased over the past century, leading to increased uptake, especially by top predators such as seabirds. Understanding the exact sources of contamination requires an individual-level approach, which is especially challenging for species that migrate. We took such an approach and located the wintering areas of 80 common terns (Sterna hirundo) through tracking, and, across years, collected feathers grown in those areas to assess their mercury levels using atomic absorption spectrometry. Although feathers of males and females did not differ in their mercury level, we found the average feather mercury level to be highest in birds wintering in the Canary Current (3.87 μg g⁻¹), medium in birds wintering in the Guinea Current (2.27 μg g⁻¹) and lowest in birds wintering in the Benguela Current (1.96 μg g⁻¹). Furthermore, we found considerable inter-annual fluctuations in feather mercury levels, a within-individual repeatability of 41%, that the mercury levels of 17% of feather samples exceeded the admitted toxicity threshold of 5 μg g⁻¹, and that the overall mean concentration of 3.4 μg g⁻¹ exceeded that of other published reports for the species. Further studies therefore should assess whether these levels lead to individual-level carry-over effects on survival and reproductive performance.

International audience | Freshwater ecosystems are the main source of water for sustaining life on earth, and the biodiversity they support is the main source of valuable goods and services for human populations. Despite growing recognition of the impairment of freshwater ecosystems by micropollutant contamination, different conceptual and methodological considerations can newly be addressed to improve our understanding of the ecological impact into these ecosystems. Here, we originally combined in situ ecotoxicology and community ecology concepts to unveil the mechanisms structuring macroinvertebrate communities along a regional contamination gradient. The novelty of our study lies in the use of an innovative biomonitoring approach (measurement of metal contents in caged crustaceans) allowing to quantify and compare on a regional scale the levels of bioavailable metal contamination to which stream communities are exposed. We were hence able to identify 23 streams presenting a significant gradient of bioavailable metal contamination within the same catchment area in the South West of France, from which we also obtained data on the composition of resident macroinvertebrate communities. Analyses of structural and functional integrity of communities revealed an unexpected decoupling between taxonomic and functional diversity of communities in response to bioavailable metal contamination. We show that despite the negative impact of bioavailable metal contamination exposure on taxonomic diversity (with an average species loss of 17% in contaminated streams), functional diversity is maintained through a process of non-random species replacement by functional redundant species at the regional scale. Such unanticipated findings call for a deeper characterization of metal-tolerant communities' ability to cope with environmental variability in multistressed ecosystems.

International audience | Multiple reaction monitoring (MRM) mass spectrometry is emerging as a relevant tool for measuring customized molecular markers in freshwater sentinel species. While this technique is typically used for the validation of protein molecular markers preselected from shotgun experiments, recent gains of MRM multiplexing capacity offer new possibilities to conduct large-scale screening of animal proteomes. By combining the strength of active biomonitoring strategies and MRM technologies, this study aims to propose a new strategy for the discovery of candidate proteins that respond to environmental variability. For this purpose, 249 peptides derived from 147 proteins were monitored by MRM in 273 male gammarids caged in 56 environmental sites, representative of the diversity of French water bodies. A methodology is here proposed to identify a set of customized housekeeping peptides (HKPs) used to correct analytical batch effects and allow proper comparison of peptide levels in gammarids. A comparative analysis performed on HKPs-normalized data resulted in the identification of peptides highly modulated in the environment and derived from proteins likely involved in the environmental stress response. Overall, this study proposes a breakthrough approach to screen and identify potential proteins responding to relevant environmental conditions in sentinel species.

International audience | During the last decades, the coastal areas of Morocco have witnessed an intense socioeconomic development associated with a continuous population growth and urban extension. This has led to an overexploitation of coastal aquifers leading to a degradation of their water quality. In order to obtain large scale overview on the quality status of Morocco's coastal aquifers (MCA) to assist national water managers to make informed decisions, a comprehensive scrutinization of the MCA against common indicators and using unified methods is essential. In this study, databases from thirteen MCA were analyzed, using multivariate statistical approaches and graphical methods in order to investigate the degree of mineralization in each aquifer and to identify the main salinization processes prevailing in groundwater. The results showed that the dominant groundwater types are Na–Cl, Ca–Mg–Cl, Ca–Mg–SO4, Ca–Mg–HCO3 and Ca–HCO3–Cl. The Gibbs diagram and the seawater contribution (0–37%) indicate that the mineralization is mainly due to the seawater intrusion and water-rock interaction. The salinity degree diagram illustrates that almost all groundwater samples are located in the moderate to very saline zone, indicating that MCA are recharged by water from variable sources. The groundwater quality assessment shows a deterioration, particularly by seawater intrusion and significant nitrate pollution. The temporal evolution confirm that the MCA are influenced by seawater namely in the Atlantic part. The Wilcox and USSL diagram indicate that the majority of sampled water are unsuitable for irrigation uses. In addition, and by referring to the WHO and the Moroccan standards for water potability, large number of samples from the groundwaters of the MCA is not fully adequate for drinking purposes. A set of management actions (e,g., artificial recharge) are proposed in order to mitigate the effect of groundwater overexploitation and seawater intrusion to ensure the sustainability of MCA.

The assessment of trace metal content in our fish diet is important due to the adverse effect on human health. Despite the increasing interest about the fish quality, little information is available for Southern Spain, a region characterized by high seafood intake. Nine species from the Bay of Cádiz with high commercial value were selected. Similar values were measured in the nine studied species for most of the elements, except for the macroelements Ca and S, and the microelements Fe, Mn and As, which showed significant differences among species. Metal Pollution Index (MPI) did not differ among species, and it was similar to those obtained for other Atlantic and Mediterranean locations. The values measured for the nine species were below the health limits provided by World, European and Spanish legislations, indicating that, in general terms, consumption of these species is safe in the study area. | 9 páginas

International audience | Mercury (Hg) is a toxic trace element widely distributed in the environment, which particularly accumulates in top predators, including seabirds. Among seabirds, large gulls (Larus sp) are generalist feeders, foraging in both terrestrial and marine habitats, making them relevant bioindicators of local coastal Hg contamination. In the present study, we reported blood Hg concentrations in adults and chicks of four different gull species breeding on the French Atlantic coast: the European herring gull (Larus argentatus), the Lesser black-backed gull (L. fuscus), the Great black-backed gull (L. marinus) and the Yellow-legged gull (L. michahellis). We also investigated the potential role of foraging ecology in shaping Hg contamination across species, using the unique combination of three dietary tracers (carbon, nitrogen and sulfur stable isotopes) and biologging (GPS tracking). A high concentration of Hg was associated with high trophic position and a marine diet in gulls, which was corroborated by birds’ space use strategy during foraging trips. Adults of all four species reached Hg concentrations above reported toxicity thresholds. Specifically, adults of Great black-backed gulls had a high trophic marine specialized diet and significantly higher Hg concentrations than the three other species. Blood Hg was 4–7 times higher in adults than in chicks, although chicks of all species received mainly marine and high trophic position prey, which is expected to be the cause of blood Hg concentrations of toxic concern. By using both stable isotopes and GPS tracking, the present study provides compelling insights on the main feeding habits driving Hg contamination in a seabird assemblage feeding in complex coastal environments.

International audience

International audience | Diffuse pollution of the environment by pesticides has become a major soil threat to non-target organisms, such as earthworms for which declines have been reported. However some endogeic species are still abundant and persist in intensively cultivated fields, suggesting they become tolerant to long-term anthropogenic pressure. We thus considered the working hypothesis that populations of Aporrectodea caliginosa earthworms from conventionally managed fields developed a tolerance to pesticides compared with those from organically managed fields. To investigate this hypothesis, we studied earthworm populations of the same genetic lineage from soils that were either lowly or highly contaminated by pesticides to detect any constitutive expression of differentially expressed molecular pathways between these populations. Earthworm populations were then experimentally exposed to a fungicide-epoxiconazole-in the laboratory to identify different molecular responses when newly exposed to a pesticide. State-of-the-art omics technology (RNA sequencing) and bioinformatics were used to characterize molecular mechanisms of tolerance in a non-targeted way. Additional physiological traits (respirometry, growth, bioaccumulation) were monitored to assess tolerance at higher levels of biological organization. In the present study, we generated the de novo assembly transcriptome of A. caliginosa consisting of 64,556 contigs with N50 = 2862 pb. In total, 43,569 Gene Ontology terms were identified for 21,593 annotated sequences under the three main ontologies (biological processes, cellular components and molecular functions). Overall, we revealed that two same lineage populations of A. caliginosa earthworms, inhabiting similar pedo-climatic environment, have distinct gene expression pathways after they long-lived in differently managed agricultural soils with a contrasted pesticide exposure history for more than 22 years. The main difference was observed regarding metabolism, with upregulated pathways linked to proteolytic activities and the mitochondrial respiratory chain in the highly exposed population. This study improves our understanding of the long-term impact of chronic exposure of soil engineers to pesticide residues.

International audience

International audience | Glass manufacturing operations lead to an increasing number of abandoned slag heaps contaminated with metallic trace elements (MTE). However, the relative influence of edaphic factors on the biodiversity of glasswork wastelands is still poorly understood although closely related to sustainable land management practices. Therefore, the objectives of this research were to provide new insights into glasswork wastelands through the investigation of (i) Orthoptera, diurnal Lepidoptera, plant communities, and (ii) abiotic parameters in the topsoils. To that end, biodiversity indices were computed from ecological inventories performed on the herbaceous layer. In addition, soil samples were taken from the topsoil layer (0-10 cm) to assess agronomic properties, actually (CEC-exchangeable) and potentially bioavailable MTE fractions (DTPA-extractable) and pseudo-total MTE contents. On the one hand, the studied site was able to support a substantially higher than excepted biodiversity with orthopteran assemblages similar to grasslands and a diurnal Lepidoptera diversity comparable to urban parks. We also noted a positive influence of plant richness on the diurnal Lepidoptera community structure. On the other hand, topsoil analysis revealed a severe Pb contamination (1800-3100 mg kg − 1) and a high potentially bioavailable Pb fraction (800-1300 mg kg − 1). However, CEC-exchangeable MTE concentrations were all below the analytical quantification limits. Moreover, the site was characterized by a medium soil fertility. From these results, Pb contamination does not appear to be a primary limiting factor for the establishment of these communities. We assume that glasswork wasteland ecosytems are more affected by soil fertility or land management practices. To conclude, these sites are able to provide biodiversity ecosystem services, acting as wildlife sanctuaries for Orthoptera and diurnal Lepidoptera, and strategic metals by phytoextraction in a circular economy model. Thus, wasteland management practices should consider the local-scale drivers of biodiversity in order to reach at least the zero net loss of biodiversity. ☆ This paper has been recommended for acceptance by Amit Bhatnagar.