Little is known regarding the use and ambient levels of pesticides in Chilean agricultural regions. Chlorpyrifos (CPF) is one of the most used active ingredients in Chile and in the world. However, few investigations have been conducted to understand its environmental distribution and fate. In this study, PUF disk passive air samples were deployed during similar to one year in two areas, Angol (5 sites) and Villarrica (2 sites), in the Araucania Region. The concentration in air of CPF ranged from ten to thousands of pg m(-3) (similar to 20-14 600). The highest CPF concentrations were detected at the Angol sites (similar to 14 600 pg m(3)) during period 2 (August-December 2008). These results were higher by a factor of similar to 10-15 than those detected in Villarica sites (similar to 2000 pg m(3)) in period 1 (April-July 2008). Seasonal CPF variations were observed, at both sites, within the sampling periods. Air back trajectory analyses showed that air masses from nearby agricultural zones contributed most of the CPF detected in Angol sites when the wind speed was low and at Villarrica when those sources were likely located north of the VMA site. These results provide initial data for CPF in the Chilean atmosphere and contribute new information to understanding distribution of CPF in Chilean environments. Copyright (C) 2016 Turkish National Committee for Air Pollution Research and Control. Production and hosting by Elsevier B.V. All rights reserved.

peer reviewed | Due to migration of harbour porpoises towards more polluted areas like the North Sea and their sensitivity towards pollution, there is a need for proper conservation measures for this species. As a consequence, knowledge about the pollutant’s kinetics is required. The present study is the first to investigate the kinetics of PBDEs in marine mammals using PBPK modeling as a non-destructive tool for describing the chemical’s kinetics in a protected animal species. The models were developed and parameterized using data from the literature and Black Sea harbour porpoises through computer opti- mization. The predictability of these models in time was assessed by reverse dosimetry modeling using data from North Sea porpoises (1990e2008). From these predictions, PBDE 99 levels were found to decrease the fastest, followed by PBDE 153, 47 and 100. Results show that the PBPK models can be applied for harbour porpoises from different regions and also simulate time trends.

We simulate the combined natural and pollutant-induced survival of early life stages of NEA cod and haddock, and the impact on the adult populations in response to the time of a major oil spill in a single year. Our simulations reveal how dynamic ocean processes, controlling both oil transport and fate and the frequency of interactions of oil with drifting fish eggs and larvae, mediate the magnitude of population losses due to an oil spill. The largest impacts on fish early life stages occurred for spills initiated in Feb–Mar, concomitant with the initial rise in marine productivity and the earliest phase of the spawning season. The reproductive health of the adult fish populations was maintained in all scenarios. The study demonstrates the application of a simulation system that provides managers with information for the planning of development activities and for the protection of fisheries resources from potential impacts. | An annual profile of the impacts of simulated oil spills on the Northeast Arctic cod and haddock fisherie | publishedVersion

Chile is a leading agricultural producer and thus consumer of insecticides, herbicides, and fungicides. In Molina, Central Chile, a prospective cohort has been established for studying the incidence and risk factors of chronic diseases in the adult population. Our goals were to measure airborne current use pesticides (CUPs), assess their spatial distribution and potential sources, and estimate health risks for the population in Molina. CUPs were measured using passive air samplers (PAS), deployed on six sites from October 2015 to August 2016. Thirty-eight pesticides were analyzed using high performance liquid chromatography (HPLC), but only nine of them were detected. Chlorpyrifos (CPF) was detected with air concentrations ranging from 444 to 14 624 (pg m(-3)). Diazinon, atrazine, dimethoate, metolachlor, simazine, terbuthylazine and tebuconazole were also detected; only pendimethalin had concentrations as high as those of CPF, with a maximum of 14 927 (pg m(-3)). Backward wind trajectories were used to estimate locations of potential sources contributing to airborne CUPs concentrations. Most of the exposure to CUPs was associated with local sources, while regional sources southern/eastern/western of Molina appear to contribute as secondary sources (soil evaporation followed by atmospheric transport) in spring and summer seasons. A health risk assessment using US-EPA's methodology was carried out for inhalation exposure of detected pesticides. None of the measured CUPs were associated with a hazard quotient (HQ) greater than 1, indicating no significant risk due to inhalation of pesticides in Molina's population with the exception of the group of children below 12 years old. However, further investigations are needed to evaluate others CUPs exposure route such as food consumption and dermal exposure to improve our health risk estimations. | Comisión Nacional de Investigación Científica y Tecnológica (CONICYT) 15130011-15110020 180078 1161673 Vicerrectoria de Investigacion, Pontificia Universidad Catolica de Chile 9/2014 RECETOX Research Infrastructure LM2015051 CZ.02.1.01/0.0/0.0/16_013/0001761

peer reviewed | Very few ecotoxicological studies have been performed on long-term exposure under controlled conditions, hence limiting the assessment of the impact of chronic and diffuse chemical pressures on the health of aquatic organisms. In this study, an ecotoxicoproteomic approach was used to assess the integrated response and possible acclimation mechanisms in Gammarus fossarum following chronic exposures to Cd, Cu or Pb, at environmentally realistic concentrations (i.e. 0.25, 1.5 and 5 µg/L respectively). After 10-week exposure, changes in protein expression were investigated in caeca of control and exposed males. Gel-free proteomic analyses allowed for the identification of 35 proteins involved in various biological functions, for which 23 were significantly deregulated by metal exposures. The protein deregulation profiles were specific to each metal, providing evidence for metal-specific action sites and responses of gammarids. Among the tested metals, Cu was the most toxic in terms of mortality, probably linked with persistent oxidative stress. Moulting and osmoregulation were the major biological functions affected by Cu in the long-term. In Pb-exposed gammarids, significant deregulations of proteins involved in immune response and cytoskeleton were observed. Reproduction appears to be strongly affected in gammarids chronically exposed to Cd or Pb. Besides, modified expressions of several proteins involved in energy transfer and metabolism highlighted important energetic reshuffling to cope with chronic metal exposures. These results support the fact that metallic pressures induce a functional and energetic cost for individuals of G. fossarum with potential repercussions on population dynamics. Furthermore, this ecotoxicoproteomic study offers promising lines of enquiry in the development of new biomarkers that could make evidence of long-term impacts of metals on the health of organisms.

We have investigated the occurrence, distribution, and biomagnification of per- and polyfluoroalkyl substances (PFAS) in two tropical lakes (Asejire and Eleyele) of Southwestern Nigeria, with contrasting urban intensities. Over an 8-month period, we sampled sediment and fish species (Clarias gariepinus: CIG; Oreochromis niloticus: ON; Coptodon guineensis: CG; Sarotherodon melanotheron: SM) across trophic levels, and analyzed various PFAS congeners, in addition to a select group of toxicological responses. While herbivores (SM) and benthic omnivores (CIG) at Asejire exhibited elevated levels of PFBS and PFOS, the pelagic omnivores (ON) showed a dominance of PFOS, PFDA, PFHxDA and EtFOSE in the muscle. At the Eleyele urban lake, PFAS patterns was dominated by PFBS, EtFOSE, PFPeS, PFOcDA and PFOS in the herbivores (SM, CG), EtFOSE, PFOS and PFBS in the pelagic omnivore (ON) and benthic omnivore (ClG). The estimated biomagnification factor (BMF) analysis for both lakes indicated trophic level increase of PFOS, PFUnA and PFDA at the suburban lake, while PFOS and EtFOSE biomagnified at the urban lake. We detected the occurrence of diSAMPAP and 9CL-PF3ONS, novel compounds not commonly reported, in PFAS studies at both lakes. The studied toxicological responses varied across trophic groups in both lakes with probable modulations by environmental conditions, trophic structure, and relative PFAS exposures in the lakes. The present study documents, for the first time in Nigeria, or any other African country, the role of urbanization on contaminant load into the environment and their implications for contaminant dynamics within the ecosystem and for aquatic food safety. | publishedVersion

Persistent organic pollutants (POPs), including brominated flame retardants (BFRs), perfluoroalkyl substances (PFAS) and metals, can accumulate in marine mammals and be transferred to offspring. In this study, we analyzed 64 lipophilic POPs, including four emerging BFRs, in the blubber, liver and muscle of 17 adult common minke whales (Balaenoptera acutorostrata) from the Barents Sea to investigate occurrence and tissue partitioning. In addition, the placental transfer concentration ratios of 14 PFAS and 17 metals were quantified in the muscle of nine female-fetus pairs to investigate placental transfer. Legacy lipophilic POPs were the dominating compound group in every tissue, and we observed generally lower levels compared to previous studies from 1992 to 2001. We detected the emerging BFRs hexabromobenzene (HBB) and pentabromotoluene (PBT), but in low levels compared to the legacy POPs. We detected nine PFAS, and levels of perfluorooctane sulfonate (PFOS) were higher than detected from the same population in 2011, whilst levels of Hg were comparable to 2011. Levels of lipophilic contaminants were higher in blubber compared to muscle and liver on both a wet weight and lipid adjusted basis, but tissue partitioning of the emerging BFRs could not be determined due to the high number of samples below the limit of detection. The highest muscle ΣPFAS levels were quantified in fetuses (23 ± 8.7 ng/g ww), followed by adult males (7.2 ± 2.0 ng/gg ww) and adult females (4.5 ± 1.1 ng/g ww), showing substantial placental transfer from mother to fetus. In contrast, Hg levels in the fetus were lower than the mother. Levels were under thresholds for risk of health effects in the whales. This study is the first to report occurrence and placental transfer of emerging contaminants in common minke whales from the Barents Sea, contributing valuable new data on pollutant levels in Arctic wildlife. | publishedVersion

Comparative investigations of microplastic (MP) occurrence in the global ocean are often hampered by the application of different methods. In this study, the same sampling and analytical approach was applied during five different cruises to investigate MP covering a route from the East-Siberian Sea in the Arctic, through the Atlantic, and into the Antarctic Peninsula. A total of 121 subsurface water samples were collected using underway pump-through system on two different vessels. This approach allowed subsurface MP (100 μm–5 mm) to be evaluated in five regions of the World Ocean (Antarctic, Central Atlantic, North Atlantic, Barents Sea and Siberian Arctic) and to assess regional differences in MP characteristics. The average abundance of MP for whole studied area was 0.7 ± 0.6 items/m3 (ranging from 0 to 2.6 items/m3), with an equal average abundance for both fragments and fibers (0.34 items/m3). Although no statistical difference was found for MP abundance between the studied regions. Differences were found between the size, morphology, polymer types and weight concentrations. The Central Atlantic and Barents Sea appeared to have more MP in terms of weight concentration (7–7.5 μg/m3) than the North Atlantic and Siberian Arctic (0.6 μg/m3). A comparison of MP characteristics between the two Hemispheres appears to indicate that MP in the Northern Hemisphere mostly originate from terrestrial input, while offshore industries play an important role as a source of MP in the Southern Hemisphere. The waters of the Northern Hemisphere were found to be more polluted by fibers than those of the Southern Hemisphere. The results presented here suggest that fibers can be transported by air and water over long distances from the source, while distribution of fragments is limited mainly to the water mass where the source is located. | publishedVersion

We simulate the combined natural and pollutant-induced survival of early life stages of NEA cod and haddock, and the impact on the adult populations in response to the time of a major oil spill in a single year. Our simulations reveal how dynamic ocean processes, controlling both oil transport and fate and the frequency of interactions of oil with drifting fish eggs and larvae, mediate the magnitude of population losses due to an oil spill. The largest impacts on fish early life stages occurred for spills initiated in Feb–Mar, concomitant with the initial rise in marine productivity and the earliest phase of the spawning season. The reproductive health of the adult fish populations was maintained in all scenarios. The study demonstrates the application of a simulation system that provides managers with information for the planning of development activities and for the protection of fisheries resources from potential impacts | An annual profile of the impacts of simulated oil spills on the Northeast Arctic cod and haddock fisheries | publishedVersion