This study aimed at elucidating the long-term efficiency of soil remediation where chemical stabilization of arsenic (As) contaminated soil using zerovalent iron (Fe) amendments was applied. A combination of chemical extraction and extended X-Ray absorption fine structure (EXAFS) spectroscopy technique was applied on soils collected from five laboratory and field experiments in Sweden and France. All soils were treated with 1 wt% of zerovalent Fe grit 2–15 years prior to the sampling. The results indicate that all studied soils, despite the elapsed time since their amendment with Fe grit, had substantial amounts of ferrihydrite and/or lepidocrocite. These metastable and the most reactive Fe (oxyhydr)oxides (mainly ferrihydrite) were still present in substantial amounts even in the soil that was treated 15 years prior to the sampling and contributed most to the As immobilisation in the amended soils. This increases confidence in the long-term efficiency of As immobilisation using zerovalent Fe amendments. Both applied methods, sequential extraction and EXAFS, were in line for most of the samples in terms of their ability to highlight As immobilisation by poorly crystalline Fe phases.

Plastics in the oceans can break up into smaller size and shape resembling prey or particles selected for ingestion by marine organisms. Plastic polymers may contain chemical additives and contaminants, including known endocrine disruptors that may be harmful for the marine organisms, in turn posing potential risks to marine ecosystems, biodiversity and food availability.This study assesses the presence of plastics in the contents of the gastrointestinal tract (GIT) of a commercial fish species, the Atlantic horse mackerel, Trachurus trachurus, sampled from two different fishing areas of central Mediterranean Sea. Adverse effect of plastics occurrence on T. Trachurus health were also assessed quantifying the liver expression of vitellogenin (VTG), a biomarker for endocrine disruption.A total of 92 specimens were collected and morphometric indices were analysed. A subgroup was examined for microplastics (MP < 1 mm) and macroplastics (MaP >1 cm) accumulation in the GIT and for VTG expression.Results indicated that specimens from the two locations are different in size and maturity but the ingestion of plastic is widespread, with microplastics (fragments and filaments) abundantly present in nearly all samples while macroplastics were found in the larger specimens, collected in one of the two locations. Spectroscopic analysis revealed that the most abundant polymers in MP fragments were polystyrene, polyethylene and polypropylene, whereas MP filaments were identified mainly as nylon 6, acrylic and polyester. MaP were composed mainly of weathered polyethylene or polypropylene.The expression of VTG was observed in the liver of 60% of all male specimens from both locations.The results of this study represent a first evidence that the ingestion of plastic pollution may alter endocrine system function in adult fish T. Trachurus and warrants further research.

Agricultural mulch film (AMF) is deemed an important source of microplastics (MPs) in agricultural soil (AS). However, quantitating the contribution of AMFs to MPs in farmland soil and surface water remains a considerable challenge to date. In the present study, a basic framework was developed to address these concerns. First, the concentrations of MPs in soil derived from AMF abrasion (CMP) and the total MPs from all sources in AS (CTMP) were measured. Then, the ratios of CMP to CTMP, i.e., the contribution of AMFs to MPs in AS, were calculated. The contribution of AMFs to MPs in surface water via soil erosion was calculated based on CTMP values, the ratios of CMP to CTMP, soil erosion intensities (SEIs), and farmland areas. Furthermore, the potential contribution of soil erosion to MPs in the ocean was estimated. In China, the inventory of MPs in surface AS in 2018 ranged from 4.9 × 10⁶ to 1.0 × 10⁷ tons according to our results. AMFs contributed 10%–30% of the CTMP with certainties of 60–95%. Assuming that all MPs in AS can be exhaustively transferred to surface water via soil erosion, the national mass transfer amount of MPs (MTTMP) from AS to surface water reached 1.2 × 10⁵−2.2 × 10⁵ tons (∼2% of the inventory of MPs in the AS of China); the fluxes of MPs into the ocean from AS were 3.4 × 10⁴−6.6 × 10⁴ tons, assuming that all MPs in the AS of coastal provinces enter the ocean. It is likely that AMFs contributed 10%–30% MTTMP and fluxes of MPs to the ocean according to the ratios of CMP to CTMP. Apparently, approximately 30% of the national MTTMP (i.e., the rate of MP flux to the ocean to MTTMP) was input to the ocean.

The physico-chemical properties of submicron aerosols were measured in the spring of 2018 and 2019 over the Yellow Sea onboard the Gisang 1 research vessel. Aerosol number concentrations in 2019 were slightly higher than those in 2018, and the mean number concentrations of particles larger than 10 nm and cloud condensation nuclei (CCN) at 0.6% supersaturation (S) in spring 2019 were 7312 ± 3807 cm⁻³ and 4816 ± 1692 cm⁻³, respectively. Aerosol concentrations in June were lower than those in April and May, which was considered to be due to the East Asian summer monsoon. Aerosol number concentrations and size distributions were significantly influenced by meteorological conditions, such as wind and relative humidity. Aitken and accumulation mode particles dominated the aerosol number size distributions over the Yellow Sea. A distinct new particle formation (NPF) and growth event was observed, the spatial extent of which was estimated to cover at least 200 km × 400 km of the Yellow Sea. The general characteristics of NPF and growth over the Yellow Sea were similar to those in rural areas. Aerosol number concentrations below 1000 cm⁻³ were recorded on extremely clean days. A CCN closure experiment conducted using previous measurement data showed good results, indicating that CCN concentrations can be estimated with good accuracy, and the hygroscopicity over the Yellow Sea was similar to that of aged continental aerosols.

Biomagnification of mercury (Hg) in the Scotia Sea food web of the Southern Ocean was examined using the stable isotope ratios of nitrogen (δ¹⁵N) and carbon (δ¹³C) as proxies for trophic level and feeding habitat, respectively. Total Hg and stable isotopes were measured in samples of particulate organic matter (POM), zooplankton, squid, myctophid fish, notothenioid fish and seabird tissues collected in two years (austral summers 2007/08 and 2016/17). Overall, there was extensive overlap in δ¹³C values across taxonomic groups suggesting similarities in habitats, with the exception of the seabirds, which showed some differences, possibly due to the type of tissue analysed (feathers instead of muscle). δ¹⁵N showed increasing enrichment across groups in the order POM to zooplankton to squid to myctophid fish to notothenioid fish to seabirds. There were significant differences in δ¹⁵N and δ¹³C values among species within taxonomic groups, reflecting inter-specific variation in diet. Hg concentrations increased with trophic level, with the lowest values in POM (0.0005 ± 0.0002 μg g⁻¹ dw) and highest values in seabirds (3.88 ± 2.41 μg g⁻¹ in chicks of brown skuas Stercorarius antarcticus). Hg concentrations tended to be lower in 2016/17 than in 2007/08 for mid-trophic level species (squid and fish), but the opposite was found for top predators (i.e. seabirds), which had higher levels in the 2016/17 samples. This may reflect an interannual shift in the Scotia Sea marine food web, caused by the reduced availability of a key prey species, Antarctic krill Euphausia superba. In 2016/17, seabirds would have been forced to feed on higher trophic-level prey, such as myctophids, that have higher Hg burdens. These results suggest that changes in the food web are likely to affect the pathway of mercury to Southern Ocean top predators.

Concentrations of airborne metal-rich particles are typically higher on subway platforms and in subway tunnels than in ambient air. The subway concourse is an area of direct air exchange with both platforms and the outside environment, but few researchers have measured the concentrations and composition of fine particles on subway concourses. We characterized the concentrations and composition of fine particles on six subway concourses in Nanjing, China in both summer and winter. We used a respiration rate-adjusted microenvironment exposure model to estimate the contribution of a 6-h work period to daily mean exposure to fine particulate matter of subway workers and compared the estimate with those for general indoor and outdoor workers. We found that particle concentrations were typically higher on the station concourses than in ambient air. The most abundant elements composing the particles were Fe, S, Ca, Si, and K in both subway concourses and reference ambient air, but their contents varied greatly between indoor and outdoor air. The indoor/outdoor ratios of Fe, Cu, and Mn were highest, and subway workers were disproportionately exposed to these three metals. The mean daily exposure dose to Fe was 44.8 μg for subway workers, approximately five times the exposure dose of indoor and outdoor workers. Daily exposure doses of Cu, Mn, V, Sr, As, Co, Sn, and Cr were also higher for subway workers. The quality of indoor air at subway stations is therefore of occupational health concern and strategies should be formulated to reduce worker exposure.

Eutrophication is one of the most widespread causes of biotic homogenization in freshwater ecosystems. Biotic homogenization can be characterized as reductions in local diversity (alpha) and occupation of available niches by more generalist species. Beta diversity is expected to decrease in more homogeneous communities, however, there is no consensus on how it responds to eutrophication. We used a space-for-time approach to analyze the process of biotic homogenization on diatom assemblages in response to eutrophication in tropical reservoirs ranging from oligotrophic to hypereutrophic conditions. Diatom assemblages were analyzed in phytoplankton and surface sediment from 12 reservoirs with different trophic levels. We calculated total beta diversity and turnover and nestedness components and used regressions to analyze their relationships with productivity differences (without distance effects). Total beta diversity had a positive influence of the trophic gradient, whereas turnover was not related to eutrophication. However, we found that eutrophication and lower species richness (alpha diversity) led to increasing rates of the nestedness component. We also observed that the homogenization process was not characterized by invasion of new species, but, on the contrary, by filtering nutrient-rich tolerant species also present in oligo-mesotrophic reservoirs and able to occupy available niches in the eutrophic reservoirs. These findings (increase in nestedness, decrease in alpha diversity, and development of tolerant species) suggest that biotic homogenization is leading to a simplification of diatom assemblages in tropical reservoirs, making assemblages from eutrophic and hypereutrophic reservoirs a subset of assemblages from oligotrophic and mesotrophic ones.

Substantial heterogeneities have been found in previous estimations of the risk from dietary exposures to persistent organic pollutants (POPs) in China, mainly due to spatiotemporal variations. To comprehensively evaluate the dietary risks of POPs listed in the Stockholm Convention, more than 27,580 data records from 753 reports published over the last three decades were examined. Respectively, for various food categories, the results obtained for the range of mean concentrations of POPs are as follows: total dichlorodiphenyltrichloroethanes (DDTs: 1.4–27.1 μg/kg), hexachlorocyclohexanes (HCHs: 1.8–29.3 μg/kg), polybrominated diphenyl ethers (PBDEs: 0.046–2.82 μg/kg), polychorinated biphenyls (PCBs: 0.05–7.57 μg/kg), polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD&Fs: 2.9–210 pg toxic equivalent (TEQ)/kg), perfluorooctanoic acid (PFOA: 0.02–0.97 μg/kg), perfluoroctane sulfonate (PFOS: 0.00082–2.76 μg/kg) and short-chain chlorinated paraffins (SCCPs: 64–348.92 μg/kg). Temporal decreasing trends were observed for DDTs, HCHs, PBDEs, PCDD&Fs, and PFOA, with no significant change for other POPs. Meanwhile, the estimated daily intake for adults were 75.2 ± 43.6 ng/kg/day for DDTs, 123 ± 87 ng/kg/day for HCHs, 0.37 ± 0.17 pg TEQ/kg/day for PCDD&Fs, 17.8 ± 9.5 ng/kg/day for PCBs, 3.3 ± 1.8 ng/kg/day for PBDEs, 3.6 ± 1.9 ng/kg/day for PFOA, 3.3 ± 2.0 ng/kg/day for PFOS, and 2.5 ± 1.6 μg/kg/day for SCCPs. Furthermore, non-carcinogenic risks were the highest for PCBs (0.89) and PCDD&Fs (0.53), followed by PFOA (0.18), PFOS (0.17), HCHs (0.062), SCCPs (0.025), DDTs (0.0075), and PBDEs (0.00047). These findings illustrated that exposure to POPs declined due to the control policies implemented in China, while the cumulative risk of POPs was still higher than 1, indicating continuous efforts are required to mitigate associated contamination.

Epidemiological indications connect maternal and developmental presence or exposure to pesticides with an increased risk for a spectrum of neurological trajectories. To provide pre-clinical data in support of this hypothesis, we used two distinct experimental models. First, female and male mice were fed immediately prior to mating, and the resulting pregnant dams were continously fed during gestation and lactation periods using chow pellets containing a cocktail of six pesticides at tolerable daily intake levels. Male and female offspring were then tracked for behavioral and in vivo electrophysiological adaptations. Second, a zebrafish model allowed us to screen toxicity and motor-behavior outcomes specifically associated with the developmental exposure to a low-to-high concentration range of the cocktail and of each individual pesticide. Here, we report anxiety-like behavior in aging male mice maternally exposed to the cocktail, as compared to age and gender matched sham animals. In parallel, in vivo electrocorticography revealed a decrease in gamma (40–80 Hz) and an increase of theta (6–9 Hz) waves, delineating a long-term, age-dependent, neuronal slowing. Neurological changes were not accompanied by brain structural malformations. Next, by using zebrafish larvae, we showed an increase of all motor-behavioral parameters resulting from the developmental exposure to 10 μg/L of pesticide cocktail, an outcome that was not associated with midbrain structural or neurovascular modifications as assessed by in vivo 2-photon microscopy. When screening each pesticide, chlorpyrifos elicited modifications of swimming parameters at 0.1 μg/L, while other components provoked changes from 0.5 μg/L. Ziram was the single most toxic component inducing developmental malformations and mortality at 10 μg/L. Although we have employed non-equivalent modalities and timing of exposure in two dissimilar experimental models, these outcomes indicate that presence of a pesticide cocktail during perinatal periods represents an element promoting behavioral and neurophysiological modifications. The study limitations and the possible pertinence of our findings to ecotoxicology and public health are critically discussed.

The assessment of the distribution and composition of microlitter in the sea is a great challenge. Biological indicators can be an irreplaceable tool since they measure microlitter levels in their environments in a way that is virtually impossible to replicate by direct physical measurements. Furthermore, trends can provide policymakers with statistically robust analysis. We looked into the capacity of multiple fish species to describe the distribution and composition of microlitter vertically across different compartments of the water column. A total of 502 individuals from six selected species (Scomber scombrus, Oblada melanura, Spicara smaris, Boops boops, Merluccius merluccius and Mullus barbatus) were collected on the western side of Sardinia island and allocated to three compartments: surface, mid-water and bottom. The species of the surface exhibited a higher frequency of occurrence (41.89%) of microlitter ingestion, compared to those of the mid-water and bottom (19.60%; 22.58%). A significant difference in the average number of ingested microlitter was found between the surface and the bottom compartment. All the microlitter fragments found were analysed through Fourier Transform Infrared Spectroscopy (FTIR). The comparison of the expected buoyancies of the polymers identified puth faith in the allocation of the species to the respective compartments. Therefore, considering the Marine Strategy Framework Directive objective, this approach could be useful in assessing microlitter distribution and composition vertically across the water column.