International audience | Sea turtles are considered as bio-indicators for monitoring the efficiency of restoration measures to reduce marine litter impacts on health. However, the lack of extended and standardised empirical data has prevented the accurate analysis of the factors influencing litter ingestion and the relationships with individual health. Historic data collected from 1988 and standard data collected from 2016 were harmonised to enable such analyses on necropsied loggerhead turtles (Caretta caretta) in eight Mediterranean and North-East Atlantic countries. Litter was found in 69.24 % of the 1121 individuals, mostly single-use and fishing-related plastics. Spatial location, sex and life history stage explained a minor part of litter ingestion. While no relationships with health could be detected, indicating that all individuals can be integrated as bio-indicators, the mechanistic models published in literature suggest that the high proportion of plastics in the digestive contents (38.77 % per individual) could have long-term repercussions on population dynamics.

International audience | Metal pollution in rivers should not be overlooked before their entry into the sea. However, there are few studies for estimating such contamination in rivers entering the Algerian coastal waters. Semimonthly quantification of dissolved and particulate metals, near the mouths of two industrial-tainted rivers, El Harrach and Mazafran rivers, was carried out during a period of one year. All the trace metals analyzed are originating from anthropogenic sources (EF > 1.5), with higher contamination of dissolved Pb, Cd, Zn and Ni and a slight degree of contamination of particulate Cu and Zn (0 < I geo < 1). Particulate metals show a stable complex with the particulate phase (e.g. 2 < LogK d < 6). The risk assessment results indicate that particulate Pb and Zn have a 33% likelihood of toxicity for adverse biological effects. A significant toxicity effect (ΣTUi >4) of the combined particulate metals (Pb, Cd, Cu, Zn, Cr, Ni and As) was primarily due to the higher particulate Cd, Zn, and Cr availability.

peer reviewed | The northern fulmar Fulmarus glacialis ingests a larger number of (micro)plastics than many other seabirds due to its feeding habits and gut morphology. Since 2002, they are bioindicators of marine plastics in the North Sea region, and data are needed to extend the programme to other parts of their distribution areas, such as the Arctic. In this study, we provide data on ingested plastics by fulmars collected in 1997 in Kongsfjorden, Svalbard. An extraction protocol with KOH was used and for half of the birds, the gizzard and the proventricular contents were analysed separately. Ninety-one percent of the birds had ingested at least one piece of plastic with an average of 10.3 (±11.9 SD) pieces. The gizzards contained significantly more plastics than the proventriculus. Hard fragments and polyethylene were the most common characteristics. Twelve percent of the birds exceeded the EcoQO value of 0.1 g.

Contamination of aquatic ecosystems by hydrophobic organic contaminants (HOCs) is often assessed based on their concentrations in riverbed sediment and suspended particulate matter (SPM). However, total HOC concentration (CTOT) in sediment or SPM is of limited value for evaluating the exposure of benthic or pelagic organisms. The accessible HOC concentration (CAS) presents a useful parameter quantifying the overall pool of HOC in sediment or SPM available for fast partitioning to the water phase or biota. We applied a novel approach of ex situ sequential equilibrium partitioning with silicone elastomer sampler at a high sampler/SPM phase ratio to measure CAS of HOC in SPM from the Danube River. We compared CTOT and CAS in SPM and surface layer sediment collected at the same sites to evaluate whether HOC monitoring in the two matrices provides equivalent information on environmental quality. At most sites, there was a good agreement and correlation of organic carbon (OC)-normalised CTOT in SPM and sediment for polychlorinated biphenyls (PCBs) and the majority of organochlorine pesticides (OCPs). In contrast, CTOT of polycyclic aromatic hydrocarbons (PAHs) in SPM were up to a factor 10 lower in SPM than in sediment. Site-specific differences of OC-normalised CAS concentrations in SPM and sediments were observed for PCBs and OCPs, with accessibility mostly lower in SPM than in sediment. The highest accessibility in SPM was observed for PCBs, ranging between 15 and 30%. The accessibility of OCPs varied from 0 to 23%. SPM and riverbed sediment samples provide complementary but not mutually interchangeable information on HOC contamination. | acceptedVersion

International audience

International audience | More than 20 years after the Water Framework Directive was adopted, there are still major gaps in the sanitary status of small rivers and waterbodies at the head of basins. These small streams supply water to a large number of wetlands that support a rich biodiversity. Many of these waterbodies are fishponds whose production is destined for human consumption or for the restocking of other aquatic environments. However, these ecosystems are exposed to contaminants, including pesticides and their transformation products. This work aims to provide information on the distribution, diversity, and concentrations of agricultural contaminants in abiotic and biotic compartments from a fishpond located at the head of watersheds. A total of 20 pesticides and 20 transformation products were analyzed by HPLC-ESI-MS/MS in water and sediment sampled monthly throughout a fish production cycle, and in three fish species at the beginning and end of the cycle.The highest mean concentrations were found for metazachlor-OXA (519.48 +/- 56.52 ng.L-1) in water and benzamide (4.23 +/- 0.17 ng g(-1) dry wt.) in sediment. Up to 20 contaminants were detected per water sample and 26 per sediment sample. The transformation products of atrazine (banned in Europe since 2003 but still widely used in other parts of the world), flufenacet, imidacloprid (banned in France since 2018), metazachlor, and metolachlor were more concentrated than their parent compounds. Fewer contaminants were detected in fish and principally prosulfocarb accumulated in organisms during the cycle.Our work brings innovative data on the contamination of small waterbodies located at the head of a basin. The transformation products with the highest frequency of occurrence and concentrations should be prioritized for further environmental monitoring studies, and specific toxicity thresholds should be defined. Few contaminants were found in fish, but the results challenge the widely use of prosulfocarb.

Wind energy has experienced a notable development during the last decades, driving new challenges for animal communities. Although bird collisions with wind turbines and spatial displacement due to disturbance have been widely described in the literature, other potential impacts remain unclear. In this study, we addressed the effect of turbine noise on the vocal behaviour of a threatened shrub-steppe passerine highly dependent on acoustic communication, the Dupont's lark Chersophilus duponti. Based on directional recordings of 49 calling and singing males exposed to a gradient of turbine noise level (from 15 up to 51 dBA), we tested for differences in signal diversity, redundancy, and complexity, as well as temporal and spectral characteristics of their vocalizations (particularly the characteristic whistle). Our results unveiled that Dupont's lark males varied the vocal structure when subject to turbine noise, by increasing the probability of emitting more complex whistles (with increased number of notes) and shifting the dominant note (emphasizing the longest and higher-pitched note). In addition, males increased duration and minimum frequency of specific notes of the whistle, while repertoire size and signal redundancy remain constant. To our knowledge, this is the first study reporting multiple and complex responses on the vocal repertoire of animals exposed to turbine noise and unveiling a shift of the dominant note in response to anthropogenic noise in general. These findings suggest that the Dupont's lark exhibits some level of phenotypic plasticity, which might enable the species to cope with noisy environments, although the vocal adjustments observed might have associated costs or alter the functionality of the signal. Future wind energy projects must include fine-scale noise assessments to quantify the consequences of chronic noise exposure.

International audience | Glyphosate (N-phosphonomethylglycine; GLP) and its main metabolite AMPA (aminomethylphosphonic acid), are frequently detected in relatively high concentrations in European agricultural topsoils. Glyphosate has a high sorption affinity, yet it can be detected occasionally in groundwater. We hypothesized that shrinkage cracks occurring after dry periods could facilitate GLP transport to greater depths where subsoil conditions slow further microbial degradation. To test this hypothesis, we simulated a heavy rainfall event (HRE) on a clay-rich arable soil. We applied 2.1 kg ha−1 of 100% 13C3, 15N-labeled GLP one day before the simulated rainfall event. Microbial degradation of translocated GLP over a 21-day period was assessed by quantifying 13C incorporation into phospholipid fatty acids. Microbial degradation potential and activity were determined by quantifying the abundance and expression of functional genes involved in the two known degradation pathways of GLP; to AMPA (goxA) or sarcosine (sarc). We confirmed that goxA transcripts were elevated in the range of 4.23 x 105 copy numbers g−1 soil only one day after application. The increase in AMPA associated with a rise in goxA transcripts and goxA-harboring microorganisms indicated that the degradation pathway to AMPA dominated. Based on 13C-enrichment 3 h after the HRE, fungi appeared to initiate glyphosate degradation. At later time points, Gram+-bacteria proved to be the main degraders due to their higher 13C-incorporation. Once GLP reached the subsoil, degradation continued but more slowly. By comparing GLP distribution and its microbial degradation in macropores and in the bulk soil, we demonstrated different time- and depth-dependent GLP degradation dynamics in macropores. This indicates the need for field studies in which soil properties relevant to GLP degradation are related to limiting environmental conditions, providing a realistic assessment of GLP fate in soils.

Water stored in open-pit lakes can be a water resource when the mine is closed. This study aimed to develop a reliable model to evaluate the water quality, based on the sulphate concentration, in the Reocín Mine area (Spain) by using geostatistical algo rithms. To this end, water samples were taken from the beginning of the flooding period in November 2004 until August 2020. The model showed that the sulphate concentration was highest between February 2009 and February 2012 and decreased as the flooding process progressed. The area with the highest concentration (2000 mg L-1) was the central part of the study area, where the mine is located, while in the northeast and southwest, the values from the beginning of the flooding period were lower, below 500 mg L-1. In the last obtained model, the values decreased considerably to 1300 mg L-1 in the central area and below 250 mg L-1 in the northeast and southwest areas. The modelling conducted to assess the water quality in the area of influence of the mine determined that the flooding process has little influence on the water in the rivers and streams in the area, since the sulphate concentration measured in the adjacent rivers and streams was less than 250 mg L-1, indicating that anomalous concentrations were only found in the open-pit area. It was shown that geostatistical algorithms are useful tools that can be used to model the intensity and extension of water pollutants in space over time.

International audience | The research paper is aimed to modify the human health impact assessment of Cr in soils. The current article presents the input of several critical parameters for the human health Impact Score (IShum) assessment in soils. The modification of the IShum is derived using geological data — results of neutron activation analysis of soils are used in the IShum calculation; research area is divided using the watersheds and population size and density. Watersheds reflect the local environmental conditions of the territory unlike the administrative units (geographical areas of the studied region) due to their geological independence. The calculations of the characterization factor value underestimate the influence of the population size and density on the final result. Default characterization factor values cannot be considered during the assessment of the potential human health impact for the big sparsely inhabited areas. In case of very low population density, the result will be overrated and underestimated in the opposite case. The current approach demonstrates that the geographical separation in the USEtox model should be specified. The same approach can be utilized for other geo zones due to the accessibility of this information (area size, population size, and density, geological, and landscape features). | The research paper is aimed to modify the human health impact assessment of Cr in soils. The current article presents the input of several critical parameters for the human health Impact Score ( IShum) assessment in soils. The modification of the IShum is derived using geological data — results of neutron activation analysis of soils are used in the IShum calculation; research area is divided using the watersheds and population size and density. Watersheds reflect the local environmental conditions of the territory unlike the administrative units (geographical areas of the studied region) due to their geological independence. The calculations of the characterization factor value underestimate the influence of the population size and density on the final result. Default characterization factor values cannot be considered during the assessment of the potential human health impact for the big sparsely inhabited areas. In case of very low population density, the result will be overrated and underestimated in the opposite case. The current approach demonstrates that the geographical separation in the USEtox model should be specified. The same approach can be utilized for other geo zones due to the accessibility of this information (area size, population size, and density, geological, and landscape features).