Coastal environments, essential for about half of the world's population living near coastlines, face severe threats from human-induced activities such as intensified urbanization, aggressive development, and particularly, coastal sewage pollution. This type of pollution, comprising untreated sewage discharging nutrients, pathogens, heavy metals, microplastics, and organic compounds, significantly endangers these ecosystems. The issue of sewage in coastal areas is complex, influenced by factors like inadequate sewage systems, septic tanks, industrial and agricultural runoff, and natural processes like coastal erosion, further complicated by oceanic dynamics like tides and currents. A global statistic reveals that over 80 % of sewage enters the environment without treatment, contributing significantly to nitrogen pollution in coastal ecosystems. This pollution not only harms marine life and ecosystems through chemical contaminants and eutrophication, leading to hypoxic zones and biodiversity loss, but also affects human health through waterborne diseases and seafood contamination. Additionally, it has substantial economic repercussions, impacting tourism, recreation, and fisheries, and causing revenue and employment losses. Addressing this issue globally involves international agreements and national legislations, but their effectiveness is hindered by infrastructural disparities, particularly in developing countries. Thus, effective management requires a comprehensive approach including advanced treatment technologies, stringent regulations, regular monitoring, and international cooperation. The international scientific community plays a crucial role in fostering a collaborative and equitable response to this pressing environmental challenge.

The increasing threats to ecosystems and humans from marine plastic pollution require a comprehensive assessment. We present a plastisphere case study from Reunion Island, a remote oceanic island located in the Southwest Indian Ocean, polluted by plastics. We characterized the plastic pollution on the island's coastal waters, described the associated microbiome, explored viable bacterial flora and the presence of antimicrobial resistant (AMR) bacteria. Reunion Island faces plastic pollution with up to 10,000 items/km2 in coastal water. These plastics host microbiomes dominated by Proteobacteria (80 %), including dominant genera such as Psychrobacter, Photobacterium, Pseudoalteromonas and Vibrio. Culturable microbiomes reach 107 CFU/g of microplastics, with dominance of Exiguobacterium and Pseudomonas. Plastics also carry AMR bacteria including β-lactam resistance. Thus, Southwest Indian Ocean islands are facing serious plastic pollution. This pollution requires vigilant monitoring as it harbors a plastisphere including AMR, that threatens pristine ecosystems and potentially human health through the marine food chain.

International audience | Environmental assessment, carried out through the life cycle assessment (LCA) method applied to NBS, presents numerous challenges. The case study involved a constructed wetland with two stages of vertical flow (French system), incorporating a complete mass balance (N, P, and C cycles) accounting for emissions to water (output of the filter), air (direct emissions), and soil (sludge spreading and reed biomass composting). Initial results indicate that the mass balance plays a crucial role in the environmental impacts. Therefore, as a recommendation, it cannot be omitted when conducting such an LCA. However, simplified emission factors can be proposed. Indirect emissions from sludge field fertilizing should also be taken into account, along with the impacts of avoided mineral fertilizers. This should provide guidelines in cases where simplifying the data gathering for LCA is considered, without a significant loss of information.

The bioaccumulation potential of contaminants in marine environments was investigated in biofilms and compared with caged mussels for a wide range of both organic and metallic contaminants across a large geographic area. Marine biofilms were sampled after three months of sub-surface immersion at 49 locations along the 1,800 km of the French Mediterranean coast. Ten chemical elements (i.e. As, Cd, Co, Cr, Cu, Hg, Mn, Ni, Pb, and Zn) and 57 organic compounds (i.e., 18 polycyclic aromatic hydrocarbons (PAHs), 8 dioxin-like and 6 non-dioxin-like polychlorinated biphenyls (PCBs) and 25 organochlorine pesticides (OCPs)) were quantified in triplicates, revealing different multi-contaminated profiles depending on sites. Most of contaminants exhibited higher concentrations in biofilms than in mussels. Moreover, a remarkable significant and positive correlation between the concentrations in both biological matrices was observed for PAHs and PCBs, and more contaminant-dependent for OCPs and metals. These results highlighted the potential of biofilms as relevant bioindicators of the marine chemical contamination.

Anthropogenic stressors can have an impact in a broad range of physiological processes and can be a major selective force leading to rapid evolution and local population adaptation. In this study, three populations of the invasive crayfish Procambarus clarkii were investigated. They are geographically separated for at least 20 years, and live in different abiotic environments: a freshwater inland lake (Salagou lake) with no major anthropogenic influence and two other coastal wetlands regularly polluted by pesticides along the Mediterranean coast (Camargue region and Bages-Sigean lagoon). Collected adults were genetically characterized using the mitochondrial COI gene and haplotype frequencies were analyzed for genetic variability within and between populations. Results revealed a higher genetic diversity for these invasive populations than any previous report in France, with more than seven different haplotypes in a single population. The contrasting genetic diversity between the Camargue and the other two populations suggest different times and sources of introduction. To identify differences in key physiological responses between these populations, individuals from each population were maintained in controlled conditions. Data on oxygen consumption rates indicate that the Salagou and Bages-Sigean populations possess a high inter-individual variability compared to the Camargue population. The low individual variability of oxygen consumption and low genetic diversity suggest a specific local adaptation for the Camargue population. Population-specific responses were identified when individuals were exposed to a pesticide cocktail containing azoxystrobin and oxadiazon at sublethal concentrations. The Salagou population was the only one with altered hydro-osmotic balance due to pollutant exposure and a change in protease activity in the hepatopancreas. These results revealed different phenotypic responses suggesting local adaptations at the population level.

International audience | Biomonitoring of persistent pesticides in birds of prey has been carried out for decades, but few studies have investigated their relevance for the monitoring of non-persistent pesticides. Herein, we determined the contamination patterns of multiple pesticides in Montagu's harrier (Circus pygargus) chicks in an intensive farming area of southwestern France. Blood samples from 55 chicks belonging to 22 nests in 2021 were assessed for 104 compounds (herbicides, fungicides, insecticides, safeners and synergists). All chicks had at least one herbicide in their blood, and half had at least two compounds. The 28 compounds detected comprised 10 herbicides, 12 fungicides, 5 insecticides and 1 synergist. Mixtures in blood were predominantly composed of herbicides, and six chicks presented a mixture of the three pesticide classes. The most prevalent compounds were sulcotrione (96% of chicks), tebutam (44%) and chloridazon (31%), of which the latter two had been banned in France for 19 and 3 years, respectively, at the time of sampling. Most compounds are considered non-acutely toxic, but sulcotrione is potentially carcinogenic, mutagenic and reprotoxic, raising questions about the effects on the health of nestlings. Biomonitoring of multiple pesticides through Montagu's harrier chicks in agroecosystems is clearly relevant because it reflects the general pattern of agricultural pesticide use in the study area. It also raises questions about exposure pathways in chicks, and further investigations are needed to disentangle the roles of dietary routes and maternal transfer for the established pesticide contamination patterns.

Anthropogenic pollutants are near-ubiquitous in aquatic systems. Aquatic animals such as fishes are subject to physiological stress induced by pollution present in aquatic systems, which can translate to changes in behaviour. Key adaptive behaviours such as shoaling and schooling may be subject to change as a result of physiological or metabolic stress or neurosensory impacts of pollution. This can result in fitness and ecological impacts such as increased predation risk and reduced foraging success. Here, we conducted a systematic metanalysis of the existing literature, comprising 165 studies, on the effects of anthropogenic pollution on sociability and group cohesion in fish species. Both organic (number of studies = 92, posterior mean (PM) = -0.483, p < 0.001) and inorganic (n = 24, PM = -0.697, p < 0.05) chemical pollutants, as well as light exposure (n = 21, PM = -3.176, p < 0.01) were found to reduce sociability. These pollutants did not reduce group cohesion, indicating that effects may be masked in group settings, though fewer studies were carried out on group cohesion and this is a key area for future research. Mixtures of chemical pollutants (n = 16) were found to reduce cohesion (PM = -45.42, p < 0.01), but increase sociability (PM = 46.00, p < 0.01). Evidence was found that fish may behaviourally acclimate to two forms of pollutant, namely mixed chemical pollutants (PM = -0.693, p < 0.05) and noise exposure (n = 22, PM = -4.059, p < 0.05). While aquatic systems are often subject to pollution from multiple sources and of multiple types, very few studies investigated the effects of multiple stressors concurrently. This review identifies trends in the existing literature, and highlights areas where further research is required in order to understand the behavioural and ecological impacts of anthropogenic pollutants in aquatic systems.

Polycyclic aromatic hydrocarbons (PAHs) are one of the most common groups of pollutants that have toxic and carcinogenic effects. Black alder trees (Alnus glutinosa L.) have been used to remediate contaminated soils from industrial pollutants and heavy metals; however, their usefulness for PAH remediation is unclear. In this study, we examined the response of seedlings from four alder half-sib families (genetic groups sharing the same mother but different fathers) to exposure to four PAHs—phenanthrene, pyrene, naphthalene, and fluoranthene—each at three concentrations. Plant growth parameters were evaluated, and concentration of secondary metabolites and antioxidant activity were measured. The results of the morphological parameters showed that in general, higher PAH concentrations had a more negative effect on tree vitality than lower concentrations (shoot growth reduction by up to 76%). Each half-sib family also exhibited distinct responses in total phenol content (TPC) when exposed to varying concentrations of pollutants, with reductions in TPC ranging from 4 to 52% across different genetic lineages. Enzyme activity also varied between families, pollutants, and their concentrations; for example, while phenanthrene generally increased glutathione S-transferase (GST) activity in the 13–99-1K and 38–61-7K half-sib families, it unexpectedly decreased GST levels by 23% and 29% in the seedlings of the 26–133-6K and 41–65-7K families, respectively, emphasizing the nuanced and divergent enzymatic responses observed in this study. Further secondary metabolite and antioxidant activity analysis revealed distinct variations in the way PAHs impact the defense mechanism of alder seedlings from different genetic groups—prioritizing either enzymatic or non-enzymatic systems. To sum up, analyzing the varying effects of PAHs on distinct half-sib families of alders can prove advantageous in identifying the most efficient black alder genetic families for phytoremediation purposes.

The behavior, and history of lead (Pb) contamination in the ecosystem of the Loire estuary was examined using elemental concentrations and Pb isotope data in water, sediment, bivalves, shrimps, and fish. In the estuary and in the surrounding coastal area, Pb concentrations in water and sediment decreased compared to concentrations determined in the 1980s, with concentrations ranging from 15.8 to 65.7 mg kg−1 in the surface sediment, 0.04–0.26 nM in the water column, and 48.0–77.9 mg kg−1 in suspended particles. Pb biomonitoring using blue mussels collected by the French Mussel Watch Program over the last 40 years showed a concentration decrease from 3.8 to 0.8 mg kg−1. A similar trend is observed in an estuarine sediment core. Changes in accompanying Pb isotope compositions strongly suggest a binary mixing process between Pb derived from terrigenous material and anthropogenic sources. Thus, environmental regulations restricting the release of lead into the environment contribute to a decrease in estuarine levels of this pollutant, which occurs on a decadal time scale.

International audience | In the Algerian Sahara, non-renewable groundwater resources estimated at 5 billion m3 thus constitute the indispensable support for irrigation. In the Berriane region in the north of Ghardaïa region (center of Algerian Sahara), an agricultural perimeter called Oued El Bir (300 ha in total) was officially created in 2013 downstream a wastewater treatment plant. A “frontier settlement” that proves the high value placed on this resource.As part of Massire project financed by IFAD (2018-2024), the objective of our study was to analyze the water reuse practices in this Berriane arid region, i.e. to study the importance of the institutional management of the treated wastewater reuse on the one hand. And on the other hand to show that the malfunctioning of the wastewater treatment plants can lead to many side effects such as the infiltration of raw sewage into the groundwater, illicit untreated wastewater irrigation and a slowdown in the development of the agricultural perimeter. Our research methodology is founded, firstly, on interviews with the local stakeholders (about 20 surveys with farmers and local institutions) and, secondly, on isotopic, chemical and biological water analyses that will be carried out soon to validate the hypothesis of infiltration of wastewater into the aquifer. In addition, to address the institutional opposition and issues relating to the technical sustainability of water reuse in the region, we implemented a water reuse demonstrator. The choice of location and equipment was determined in concertation with local stakeholders. The decentralized treatment process is based on a septic tank and a fixed bacterial filter (Advanced Enviro Septic) with 10 m3/day capacity. The treated wastewaters are used to irrigate a 1 ha surface of the new oasis area. As part of our study, we are investigating the sanitary and agronomic safety of this new treatment process for water reuse in the arid context.