Pesticides, in particular insecticides, can be very beneficial but have also been found to have harmful side effects on non-target insects. Butterflies play an important role in ecosystems, are well monitored and are recognised as good indicators of environmental health. The amount of information already known about butterfly ecology and the increased availability of genomes make them a very valuable model for the study of non-target effects of pesticide usage. The effects of pesticides are not simply linear, but complex through their interactions with a large variety of biotic and abiotic factors. Furthermore, these effects manifest themselves at a variety of levels, from the molecular to metapopulation level. Research should therefore aim to dissect these complex effects at a number of levels, but as we discuss in this review, this is seldom if ever done in butterflies. We suggest that in order dissect the complex effects of pesticides on butterflies we need to integrate detailed molecular studies, including characterising sequence variability of relevant target genes, with more classical evolutionary ecology; from direct toxicity tests on individual larvae in the laboratory to field studies that consider the potentiation of pesticides by ecologically relevant environmental biotic and abiotic stressors. Such integration would better inform population-level responses across broad geographical scales and provide more in-depth information about the non-target impacts of pesticides.

Mercury (Hg) is a pervasive environmental contaminant that can impair avian health, consequently there is a need to gauge exposures. Bird blood provides a measure of recent dietary exposure to Hg, but blood collection and storage can be complex and costly. Dried blood spots (DBS) may help overcome challenges of whole blood analyses, therefore, this study aimed to develop and validate a novel method to assess Hg exposure in birds using DBS. First, accuracy and precision of blood Hg concentrations for entire DBS and DBS punches were determined for white leghorn chicken (Gallus gallus domesticus) dosed with methylmercury (MeHg) via egg injection. Next, we investigated Hg stability in chicken DBS subjected to time, temperature, and humidity treatments. Lastly, we applied the method to DBS created using standard field methods from zebra finch (Taeniopygia guttatato) in the laboratory and American golden-plover (Pluvalis dominica) sampled in the field. All samples were analyzed for total Hg (THg) using direct Hg analysis. Accuracy was determined by comparing DBS concentrations with those of corresponding whole blood and reported as percent recovery. Accuracy for entire chicken DBS was 101.8 ± 5.4%, while DBS punches revealed lower recovery (87.7 ± 4.0 to 92.4 ± 4.1%). There was little effect of time, temperature, and humidity storage treatments on Hg concentrations of DBS, with mean DBS THg concentrations within ±8% of whole blood (n = 10 treatments). For zebra finch, DBS punches were more accurate (93.7 ± 9.7%) compared to entire DBS (126.8 ± 19.4%). While for American golden-plover, entire DBS resulted in the most accurate THg concentrations (111.5 ± 7.6%) compared to DBS punches (edge: 115.4 ± 18.9%, interior: 131.4 ± 16.1%). Overall, results indicate that DBS analysis using direct Hg analysis can accurately evaluate Hg exposure in birds.

Activated sludge (AS) has been regarded as the main driver in the removal of organic pollutants such as pesticides due to a high diversity and abundance of microorganisms. However, little is known about the biodegradation genes (BDGs) and pesticide degradation genes (PDGs) harbored in the AS from wastewater treatment plants (WWTPs). In this study, we explored the bacterial communities and BDGs/PDGs in the AS from five WWTPs affiliated with pesticide factories across four consecutive seasons based on high-throughput sequencing. The AS in pesticide WWTPs exhibited unique bacterial taxa at the genus level. Furthermore, a total of 17 BDGs and 68 PDGs were explored with a corresponding average relative abundance of 0.002–0.046% and 2.078–7.143% in each AS sample, respectively, and some BDGs/PDGs clusters were also identified in the AS. The bacterial communities and BDGs/PDGs were season-dependent, and the total variations of 50.4% and 76.8% were jointly explained by environmental variables (pesticide types, wastewater characteristics, and temperature). In addition, network analysis and distribution patterns suggested that the potential hosts of BDGs/PDGs were Thauera, Stenotrophomonas, Mycobacterium, Hyphomicrobium, Allochromatium, Ralstonia, and Dechloromonas. Our findings demonstrated the linkages of bacterial communities and BDGs/PDGs in the AS, and depended on the seasons and the pesticide wastewater characteristics.

Inorganic arsenic exposure may be associated with diabetes, but the evidence at low-moderate levels is not sufficient. Polymorphisms in diabetes-related genes have been involved in diabetes risk. We evaluated the association of inorganic arsenic exposure on diabetes in the Hortega Study, a representative sample of a general population from Valladolid, Spain. Total urine arsenic was measured in 1451 adults. Urine arsenic speciation was available in 295 randomly selected participants. To account for the confounding introduced by non-toxic seafood arsenicals, we designed a multiple imputation model to predict the missing arsenobetaine levels. The prevalence of diabetes was 8.3%. The geometric mean of total arsenic was 66.0 μg/g. The adjusted odds ratios (95% confidence interval) for diabetes comparing the highest with the lowest tertile of total arsenic were 1.76 (1.01, 3.09) and 2.14 (1.47, 3.11) before and after arsenobetaine adjustment, respectively. Polymorphisms in several genes including IL8RA, TXN, NR3C2, COX5A and GCLC showed suggestive differential associations of urine total arsenic with diabetes. The findings support the role of arsenic on diabetes and the importance of controlling for seafood arsenicals in populations with high seafood intake. Suggestive arsenic-gene interactions require confirmation in larger studies.

Biomass derived biochar is a stable carbon-rich product with potential for soil amendment. Introduced into the natural environment, biochar will naturally experience ‘ageing’ processes that are liable to change its physicochemical properties and the mobility of sorbed pollutants over the longer term. To elucidate the reciprocal effects of biochar ageing and heavy metal adsorption on the affinity of biochar for organic pollutants, we systematically assessed the adsorption of diethyl phthalate (DEP), representative of phthalic acid esters (PAEs), to fresh and aged biochars with and without coexistence of Cd²⁺. Long-term oxidative ageing was simulated using 5% H₂O₂ and applied to biochar samples made from corn cob, maize straw and wheat straw made by pyrolysis at both 450 °C and 650 °C. Our results showed that biochar made at lower temperature (450 °C) and from straw exhibited the higher adsorption capacity, owing to their greater polarity and abundance of O-containing functional groups. The adsorption of DEP onto fresh biochars was found to be driven by van der Waals force and H-bonding. Biochar made at the higher temperature (650 °C) displayed higher carbon stability than that produced at lower pyrolysis temperature. Oxidized biochar showed lower adsorption capacity than fresh biochar owing to the formation of three-dimensional water clusters on biochar surface, which blocked accessible sites and decreased the H-bonding effect between DEP and biochars. The coexistence of Cd²⁺ suppressed the sorption of DEP, via competition for the same electron-rich sites. This indicates that cation/π-π EDA interactions are the primary mechanism for PAE and Cd²⁺ stabilization on biochar. Our study sheds light on the mechanism of organic pollutant sorption by biochar, as well as the potential susceptibilities of this sorption to ageing effects in the natural environment.

A limitation to recycling wood from construction and demolition (C&D) waste is contamination of metals from the inadvertent inclusion of preservative treated wood, in particular wood treated with chromated copper arsenate (CCA) and newer copper-based formulations. To minimize contamination many regions have developed best management practices (BMPs) for separating treated from untreated wood. The objective of this study was to evaluate the fraction of preservative treated wood in recycled C&D wood after the implementation of BMPs, using Florida as a case study. Methods involved collecting recycled C&D wood samples from throughout the state, measuring metals concentrations (As, Cu, and Cr) in the samples to compute the fraction of recycled wood treated with waterborne wood preservatives, and comparing measurements with those taken prior to the formalization of BMPs. Metals concentrations were measured using two methods, one based on traditional laboratory digestion methods and another using a more rapid hand-held X-ray Fluorescence (XRF) device in the field. The proportion of waterborne preservative-treated wood in recycled wood products has reduced significantly in the intervening 20 years (from 6% to 2.9%), and the fraction of CCA-treated wood has been reduced even further, to 1.4%. The remaining fraction of waterborne preservative-treated wood is comprised of new formulations of copper-based preservatives. This suggests that restrictions from the wood preservation industry and best management practices implemented at recycling facilities have been effective in reducing heavy metal contamination from pressure treated lumber in recycled wood products.

Microplastic pollution is apparent throughout the marine environment from deep ocean sediments to coastal habitats. Most of this is believed to originate on land, although marine activities, such as fishing and shipping, also contribute to the release and redistribution of microplastic. The relative importance of these maritime plastic sources, the manner by which they are distributed in the environment, and their effect on uptake by marine organisms are yet to be fully quantified. In this study, the relative impact of fishing activities on microplastic uptake by demersal fish and crustaceans was explored. Local fishing intensity, proximity to land and mean water velocity are compared to microplastic uptake in plaice, Pleuronectes platessa, and spider crab, Maja squinado, from the Celtic Sea. Observations were also made of microplastic contamination in ingested sand eels, Ammodytes tobianus, to establish a potential route of trophic transfer. This study is the first to identify microplastic contamination in spider crab and to document trophic transfer in the wild. Individuals were sampled from sites of varied fishing intensity in the Celtic Sea, and their stomach contents examined for the presence of microplastic. Contamination was observed in 50% of P. platessa, 42.4% of M. squinado, and 44.4% of A. tobianus. Locations of highest plastic abundance varied between P. platessa and M. squinado, indicating that different factors influence the uptake of microplastic in these two taxa. No significant link was observed between fishing effort and microplastic abundance; however, proximity to land was linked to increased abundance in M. squinado and Observations of whole prey demonstrate ongoing trophic transfer from A. tobianus to P. platessa. The lack of significant difference in microplastic abundance between predator and prey suggests that microplastic is not retained by P. platessa.

While increasing evidence suggested that PM₂.₅ is the most harmful fraction of the particle pollutants, the health effects of coarse particles (PM₁₀–₂.₅) have been inconclusive, especially on cerebrovascular diseases, we thus evaluated the effects of PM₁₀, PM₂.₅, and PM₁₀–₂.₅ on stroke mortality in six Chinese subtropical cities using generalized additive models. We also conducted random-effects meta-analyses to estimate the overall effects across the six cities. We found that PM₁₀, PM₂.₅, and PM₁₀₋₂.₅ were significantly associated with stroke mortality. Each 10 μg/m³ increase of PM₁₀, PM₂.₅ and PM₁₀₋₂.₅ (lag03) was associated with an increase of 1.88% (95% CI: 1.37%, 2.39%), 3.07% (95% CI: 2.35%, 3.79%), and 5.72% (95% CI: 3.82%, 7.65%) in overall stroke mortality. Using the World Health Organization's guideline as reference concentration, we estimated that 3.21% (95% CI: 1.65%, 3.01%) of stroke mortality (corresponding to 1743 stroke mortalities, 95% CI: 896, 1633) were attributed to PM₁₀, 5.57% (95% CI: 0.50%, 1.23%) stroke mortality (3019, 95% CI: 2286, 3777) were attributed to PM₂.₅, and 2.02% (95% CI: 1.85%, 3.08%) of stroke mortality (1097, 95% CI: 1005, 1673) could be attributed to PM₁₀₋₂.₅. Our analysis indicates that both PM₂.₅ and PM₁₀₋₂.₅ are important risk factors of stroke mortality and should be considered in the prevention and control of stroke in the study area.

Persistent pollutants such as organochlorine compounds (OCs) have been highlighted as a cause of population decline in avian predators. Understanding patterns of OCs contamination can be crucial for the conservation of affected species, yet little is known on these threats to African raptors. Here we report on OC concentrations in an endangered predator endemic to southern Africa, the Black Harrier Circus maurus. Blood samples were collected in 2012–2014 from wild nestlings (n = 90) and adults (n = 23) in south-western South Africa, where agriculture and urbanization have developed rapidly since the 1950s. Polychlorinated biphenyl (ΣPCB) and dichlorodiphenyltrichloroethane (ΣDDT, for p,p’-DDT + p,p’-DDE) were detected in 79% and 84% of sampled individuals, respectively, with varying concentrations among demographic groups: nestlings had significantly higher ΣPCB and p,p’-DDT concentrations than adults, while adults had higher levels of p,p’-DDE than nestlings. Levels of ΣPCB significantly increased with an index of electric transformer density, a measure of the number and power of electric transformers around active nests. We propose this index as a useful tool for assessing ΣPCB exposure risk in other wildlife. Levels of p,p’-DDE significantly increased with the proportion of wetlands within the breeding territory, and also with the proportion of bird biomass in the diet. No association was found between OC levels and the protected area status of nesting sites. Physiological effects of contaminants were also manifest in increased white blood cell counts with higher p,p’-DDT levels. Heterophil to lymphocyte ratio increased with higher ΣPCB levels, suggesting increased physiological stress and reduced immunity in contaminated individuals. Our results suggest that OCs are still a current cause of concern for endangered Black Harriers, as well as other sympatric predators.

Although many previous studies have reported the soil pH and organic matter to be the most critical factors that affect the transfer of Cd in soil-crop systems in temperate zones, the behavior of Cd transfer is different in the Pearl River Delta (PRD), which is located in a subtropical zone with different climate and soil conditions. Therefore, we must determine the critical environmental factors that influence the transfer of Cd in the soil-vegetable system in the PRD region. Such knowledge can improve the safety of vegetables. In this study, the soil geochemical properties are investigated to explore the key soil factors that control the uptake of Cd by flowering cabbage, a popular leaf vegetable in China, from soils in the PRD region. The Cd contents in vegetables were most positively correlated to soil oxalate-Cd (p < 0.01), which indicates that amorphous Cd is the most available form for uptake into the cabbages. With the characteristics of rich in Fe oxide and Al oxide in the PRD soils, soil Fe and Al oxides were found to be the most relevant to the transfer factors of Cd from the soils to the cabbages. Soil secondary minerals are the key factor that affects the transfer of Cd, thereby influencing the migration and fate of Cd in soil-cabbage systems, with DCB-Fe significantly decreasing the Cd accumulation in cabbages. Additionally, models were developed to predict the enrichment of Cd in flowering cabbages, in which oxalate-Cd, DCB-Fe, and NaOAc-Al in soils were determined to be the most important factors that affect the Cd enrichment in flowering cabbages. In this study, we determine the important role of soil secondary minerals in affecting the transfer of Cd in soil-cabbage systems in the PRD. These observations are important to evaluate the accumulation of Cd in vegetables in subtropical zones.