To better understand the Hg(II) adsorption by some typical soils and explore the insights about the binding between Hg(II) and soils, a batch of adsorption and characteristic experiments was conducted. Results showed that Hg(II) adsorption was well fitted by the Langmuir and Freundlich. The maximum adsorption amount of cinnamon soil (2094.73 mg kg⁻¹) was nearly tenfold as much as that of saline soil (229.49 mg kg⁻¹). The specific adsorption of Hg(II) on four soil surface was confirmed by X-ray photoelectron spectroscopy (XPS) owing to the change of elemental bonding energy after adsorption. However, the specific adsorption is mainly derived from some substances in the soil. Fourier transform infrared spectroscopy (FTIR) demonstrated that multiple oxygen-containing functional groups (O–H, C=O, and C–O) were involved in the Hg(II) adsorption, and the content of oxygen functional groups determined the adsorption capacity of the soil. Meanwhile, scanning electron microscopy combined with X-ray energy dispersive spectrometer (SEM–EDS) more intuitive revealed the binding of mercury to organic matter, metal oxides, and clay minerals in the soil and fundamentally confirmed the results of XPS and FTIR to further elucidate adsorptive phenomena. The complexation with oxygen-containing functional groups and the precipitation with minerals were likely the primary mechanisms for Hg(II) adsorption on several typical soils. This study is critical in understanding the transportation of Hg(II) in different soils and discovering potential preventative measures.

Atmospheric N deposition is a serious problem in subtropical China where N is present in large amounts but P is deficient. Several studies hypothesized that N₂ fixers can overcome phosphorus limitation by trading fixed N₂ for soil phosphorus. In order to know whether N₂ fixers could invest fixed N₂ in extracellular phosphatase production and could stimulate arbuscular mycorrhizal fungi (AMF) to acquire soil P in N-rich subtropical China, an open-air greenhouse experiment was carried out. Two N₂ fixers (Acacia mangium and Ormosia pinnata) and two non-N₂ fixers (Schima superba and Pinus massoniana) were exposed to three levels of N addition: 5.6 kg ha⁻¹ a⁻¹ (ambient N), 15.6 kg ha⁻¹ a⁻¹ (middle N), and 20.6 kg ha⁻¹ a⁻¹ (high N). We found that the capacity of plants to acquire soil P in N-rich subtropical China is species specific. The higher P uptake rates were found for N₂ fixers than non-N₂ fixers under N addition, which were related to the greater soil APA and higher AMF (p < 0.01) in the soil of N₂ fixers. However, with time, high N addition decreased more significant quantities of soil microbial phospholipid fatty acids (PLFAs) in the soil of N₂ fixers than that of non-N₂ fixers (p < 0.05). We conclude that N₂ fixers have higher P uptake capacity than non-N₂ fixers under ambient N deposition in subtropical China. However, continuing N deposition in the future might affect P uptake ability of N₂ fixers as high N addition would decrease soil microbial PLFAs of N₂ fixers.

This study looks at the pollution status of six priority control phthalate esters (PAEs) under different cultivation of agricultural soils in the Sanjiang Plain, northeast China. Results show the total concentration of PAEs ranged from 162.9 to 946.9 μg kg⁻¹ with an average value of 369.5 μg kg⁻¹. PAE concentrations in three types of cultivated soils exhibited decreasing order paddy field (532.1 ± 198.1 μg kg⁻¹) > vegetable field (308.2 ± 87.5 μg kg⁻¹) > bean field (268.2 ± 48.3 μg kg⁻¹). Di-(2-ethylhexyl) phthalate (DEHP) and di-n-butyl phthalate (DnBP) were the most abundant PAEs congeners. Compared with previous studies, agricultural soils in the Sanjiang Plain showed relatively low contamination levels. Anthropogenic activities such as cultivation practices and industrial emissions were associated with the distribution pattern of PAEs. Furthermore, human health risks of PAEs were estimated and the non-cancer risk shown negligible but carcinogenic risk of DEHP exceeded the threshold limits value. PAE contaminants originated from cultivation practices and intense anthropogenic activities result in placing the agricultural soils under a potential risk to human health and also to ecosystems in the Sanjiang Plain. Therefore, the contamination status of PAEs in agricultural soil and potential impacts on human health should attract considerable attention.

Sediment bacterial communities are sensitive to environmental fluctuations, particularly external input sources. Studying the relationships between bacterial community distribution and the estuarine environment is critical for understanding the river-lake confluence ecosystem and the effect of inflow-rivers on lakes. In the present study, bacterial communities from the sediments of 14 estuaries and four pelagic sites of the Taihu Lake were investigated via high-throughput sequencing. The results demonstrated that Delta-, Beta-, and Gamma-proteobacteria, Acidobacteria, Nitrospira, Bacilli, Anaerolineae, and Actinobacteria were the major classes in sediment bacterial communities of the Taihu Lake. In general, the inflow-rivers of different pollution types have distinctly different influences on sediment bacterial communities of the lake. The bacterial community composition and physicochemical properties of pelagic sites were closer to those of the estuaries of western region which was polluted by serious industrial and agricultural pollution. The bacterial community diversity of estuaries was lower than those of pelagic sites. Spearman correlation analysis indicated that α-diversity of the bacterial community was significantly correlated with C/N, total nitrogen, and total carbon in estuarine sediments. Redundancy analysis revealed that the variance in bacterial community composition was also significantly associated with C/N (24.9%) followed by total phosphorus (15.8%), nitrite (7.2%), and nitrate (7.7%) among different estuaries. This study provides a reference to understand the influence of inflow-rivers on the lake ecosystem, which offered a basic guidance for maintaining the ecological system and protecting the water environment of lacustrine basin.

Recent expansion of shale and coal seam gas production worldwide has increased the need for geochemical studies in aquifers near gas deposits, to determine processes impacting groundwater quality and better understand the origins and behavior of dissolved hydrocarbons. We determined dissolved methane concentrations (n = 36) and δ¹³C and δ²H values (n = 31) in methane and groundwater from the 46,000-km² Gippsland Basin in southeast Australia. The basin contains important water supply aquifers and is a potential target for future unconventional gas development. Dissolved methane concentrations ranged from 0.0035 to 30 mg/L (median = 8.3 mg/L) and were significantly higher in the deep Lower Tertiary Aquifer (median = 19 mg/L) than the shallower Upper Tertiary Aquifer (median = 3.45 mg/L). Groundwater δ¹³CDIC values ranged from −26.4 to −0.4 ‰ and were generally higher in groundwater with high methane concentrations (mean δ¹³CDIC = −9.5 ‰ for samples with >3 mg/L CH₄ vs. −16.2 ‰ in all others), which is consistent with bacterial methanogenesis. Methane had δ¹³CCH₄ values of −97.5 to −31.8 ‰ and δ²HCH₄ values of −391 to −204 ‰ that were also consistent with bacterial methane, excluding one site with δ¹³CCH₄ values of −31.8 to −37.9 ‰, where methane may have been thermogenic. Methane from different regions and aquifers had distinctive stable isotope values, indicating differences in the substrate and/or methanogenesis mechanism. Methane in the Upper Tertiary Aquifer in Central Gippsland had lower δ¹³CCH₄ (−83.7 to −97.5 ‰) and δ²HCH₄ (−236 to −391 ‰) values than in the deeper Lower Tertiary Aquifer (δ¹³CCH₄ = −45.8 to −66.2 ‰ and δ²HCH₄ = −204 to −311 ‰). The particularly low δ¹³CCH₄ values in the former group may indicate methanogenesis at least partly through carbonate reduction. In deeper groundwater, isotopic values were more consistent with acetate fermentation. Not all methane at a given depth and location is interpreted as being necessarily produced in situ. We propose that high dissolved sulphate concentrations in combination with high methane concentrations can indicate gas resulting from contamination and/or rapid migration as opposed to in situ bacterial production or long-term migration. Isotopes of methane and dissolved inorganic carbon (DIC) serve as further lines of evidence to distinguish methane sources. The study demonstrates the value of isotopic characterisation of groundwater including dissolved gases in basins containing hydrocarbons.

The present work analyzed three hard-bottom and two soft-bottom species of sabellid polychaetes to determine the content of several heavy metals in their branchial crown and body. The highest concentrations of heavy metals were recorded in the hard-bottom species Branchiomma bairdi, a recent Mediterranean introduction. Differences in the metal concentrations were most notable in the high trace metal levels of the branchial crown for all the studied species. Statistical analysis showed that the Mediterranean hard-bottom species were similar each other in their heavy metal content in the body as well as in the branchial crown and appeared separated from all the other species. Arsenic and vanadium hyperaccumulation in the branchial crowns of the considered sabellid species probably acts as a deterrent for predation. The observed differences among the examined species were discussed not only at the light of habitat colonization but also in terms of the phylogeny.

The depletion of fossil fuels and hike in crude oil prices were some of the main reasons to explore new alternatives from renewable source of energy. This work presents the impact of various bowl geometries on diesel engine with diesel and biodiesel samples. Three non-edible oils were selected, namely pumpkin seed oil, orange oil and neem oil. These oils were converted into respective biodiesel using transesterification process in the presence of catalyst and alcohol. After transesterification process, the oils were termed as pumpkin seed oil methyl ester (PSOME), orange oil methyl ester (OME) and neem oil methyl ester (NOME), respectively. The engine used for experimentation was a single-cylinder four-stroke water-cooled direct-injection diesel engine and loads were applied to the engine using eddy current dynamometer. Two bowl geometries were developed, namely toroidal combustion chamber (TCC) and trapezoidal combustion chamber (TRCC). Also, the engine was inbuilt with hemispherical combustion chamber (HCC). The base line readings were recorded using neat diesel fuel with HCC for various loads. Followed by 20% of biodiesel mixed with 80% neat diesel for all prepared methyl esters and termed as B1 (20% PSOME with 80% diesel), B2 (20% OME with 80% diesel) and B3 (20% NOME with 80% diesel). All fuel samples were tested in HCC, TCC and TRCC bowl geometries under standard injection timing and with compression ratio of 18. Increased brake thermal efficiency and reduced brake specific fuel consumption were observed with diesel in TCC geometry. Also, higher heat release and cylinder pressures with lower ignition delay were recorded with TCC bowl geometry. TCC bowl geometry showed lower CO, HC and smoke emissions with B2 fuel sample than diesel and other biodiesel samples. But, higher NOx emission was observed in HCC and TCC than that in TRCC bowl geometry. Graphical abstract ᅟ

A synergistic system of dielectric barrier discharge (DBD) combined with La/Ce-TiO₂ was developed to investigate the decomposition performance of the environmentally persistent perfluorooctanoic acid (PFOA). The La/Ce-TiO₂ was modified by sol–gel method and characterized by XRD, SEM, and energy dispersive X-ray. The effects of PFOA concentration, applied voltage, initial pH, liquid conductivity, and additives on the removal rate of PFOA were explored. The results showed that the La/Ce-TiO₂ exhibited excellent catalytic effects on PFOA degradation in DBD system. When the applied voltage, PFOA concentration, pH value, and solution volume were 75 V, 100 mg/L, 3.63, and 1000 mL, respectively, the removal efficiency of PFOA was up to 97.5% by adding La₄Ce₁-TiO₂ in DBD. The corresponding defluorination ratio, TOC removal, and decomposition yield were 62.2%, 57.3%, and 37 g/kWh, respectively. Furthermore, five main intermediates including CF₃(CF₂)₆H, CF₃(CF₂)₅COOH, CF₃(CF₂)₅COH, CF₃(CF₂)₄COOH, and CF₃CF₂CF₃ were identified with LC–MS, and the degradation pathways of PFOA were proposed. The degradation mechanisms revealed that hydroxyl radicals play a significant role in the degradation of PFOA in the synergistic system.

The level of 16PAH accumulation was determined in 75 soil samples collected from two agricultural regions of Poland corresponding to the smallest Polish administrative unit at the LAU 2 level. Both regions are characterised by similar territory and soil cover but different history of pollution and different pressure of anthropogenic factors. Overall accumulation of Σ16PAHs in the upper soil layer was within a wide range with the median value of 291 and 1253 μg kg⁻¹ for a non-contaminated and high anthropopressure region, respectively. Nearly 75 % of the total polycyclic aromatic hydrocarbon (PAH) pool was represented by high molecular four-to-six-ring compounds, deriving mainly from combustion sources. The total organic carbon (Cₒᵣg) and black carbon (BC) contents were the main parameters associated with the PAH accumulation in soils, and the level of the regional anthropopressure was considered a significant factor. The strongest links of PAHs/BC (r = 0.70, p ≤ 0.05) were found in the region of high anthropopressure, characterized by a relatively high content of BC (up to 45.3 g kg⁻¹), which tends to heavily adsorb hydrocarbons. In a region of low influence exerted by anthropopressure, the PAH/Cₒᵣg or PAH/BC relationships were not observed, which may suggest different diffuse sources of PAH origin and a dominant role of other organic matter fractions in retention of PAHs in soils.

Flow cytometry has recently been presented as a research tool in the assessment of the viability/activity of activated sludge from municipal wastewater treatment plants, but it has not put in practice for industrial biotreatments yet. In this study, for the first time ever, the reliability and significance of the multiparameter flow cytometry applied to the biological nitrification-denitrification treatment of leachate have been evaluated. Using a double staining procedure (cFDA/PI), the viable, damaged, and dead subpopulations were determined, and the results were compared to those obtained with conventional methods, such as nitrogen and oxygen uptake rates or plate counting. Flow cytometry showed that viable cells represented approximately 47% of the total population, whereas active cells accounted for 90%. For both sludge from nitrification and denitrification processes, with less than 1% of them being also culturable in plate. Either flow cytometry or uptake rates revealed that health status of sludge remained constant throughout the biotreatment, which is consistent with the high recirculation rates. Under anaerobic starvation conditions, physiological status of sludge remained constant as well as specific oxygen and denitrification rates. Nevertheless, both the culturability in plate and the nitrification rate significantly decreased. These findings proved that multiparameter flow cytometry is a useful tool for the assessment of the viability and activity of sludge from a nitrification-denitrification biotreatment process. These results gathered all the bacterial communities in the sludge, so the decay in minority populations, such as nitrifying bacteria, requires the use of a complementary technique to evaluate specific activities.