Coral reefs are amongst the most biodiverse ecosystems on earth, but are significantly impacted by agricultural runoff. Despite herbicides being commonly detected in coastal waters, the possibility of herbicide accumulation in coral reef species has largely been overlooked. We investigate the accumulation of several herbicides in five species of coral reef invertebrates collected from ten sites along the Maputaland coast, South Africa. Multiple herbicide residues were detected in 95% of the samples, with total average concentrations across sites ranging between 25.2 ng g⁻¹ to 51.3 ng g⁻¹ dw. Acetochlor, alachlor and hexazinone were the predominant herbicides detected at all sites, with atrazine and simazine detected less frequently. Significant interactive effects were detected between sites nested in reef complex crossed with species, based on multiple and total herbicide concentrations. In general, multivariate herbicide concentrations varied significantly between species within and across most sites. Contrastingly, the concentrations of the different herbicides and that of total herbicide did not differ between conspecifics at most sites nested in their respective reef complexes. On average, highest total herbicide concentrations were measured in soft coral (Sarcophyton glaucum; 90.4 ± 60 ng g⁻¹ and Sinularia gravis; 42.7 ± 25 ng g⁻¹) and sponge (Theonela swinhoei; 39.0 ± 40 ng g⁻¹) species, while significantly lower concentrations were detected in hard corals (Echinopora hirsutissima; 10.5 ± 5.9 ng g⁻¹ and Acropora austera; 5.20 ± 4.5 ng g⁻¹) at most sites. Agricultural runoff entering the ocean via the uMfolozi-St Lucia Estuary and Maputo Bay are likely sources of herbicide contamination to coral reefs in the region. There is an urgent need to assess the long-term effects of herbicide exposure on coral reef communities.

Urban ecosystems and remnant habitat 'islands' therein, provide important strongholds for many wildlife species including those of conservation significance. However, the persistence of these habitats can be undermined if their structure and function are too severely disrupted. Urban wetlands, specifically, are usually degraded by a monoculture of invasive vegetation, disrupted hydrology, and chronic-contamination from a suite of anthropogenic pollutants. Top predators—as bioindicators—can be used to assess and monitor the health of these ecosystems. We measured eight health parameters (e.g., parasites, wounds and scars, tail loss and body condition) in a wetland top predator, the western tiger snake, Notechis scutatus occidentalis. For three years, snakes were sampled across four wetlands along an urban gradient. For each site, we used GIS software to measure the area of different landscapes and calculate an urbanisation–landscape score. Previously published research on snake contamination informed our calculations of a metal-pollution index for each site. We used generalised linear mixed models to assess the relationship between all health parameters and site variables. We found the metal-pollution index to have the most significant association with poor body condition. Although parasitism, tail loss and wounds differed among sites, none of these parameters influenced body condition. Additionally, the suite of health parameters suggested differing health status among sites; however, our measure of contemporary landscape urbanisation was never a significant predictor variable. Our results suggest that the health of wetland predators surrounding a rapidly growing city may be offset by higher levels of environmental pollution.

Xiangjiang (XJ) is a typical urban inland river that serves as a drinking water source, which may be affected by the currently used organochlorine pesticides (CUOCPs) originating from agricultural activities in the vicinity. On this basis, this study comprehensively explored the occurrence and distribution characteristics of CUOCPs in surface water and sediments under long-term precipitation and subsequent floods. Considering the low concentration of CUOCPs in water, a technique combining high-throughput organic analysis with high-volume solid phase extraction (High-throat/Hi-volume SPE) was introduced for effective analysis of CUCOPs. The results showed that the concentration of CUOCPs in the water and sediments of XJ ranged from 2.33 to 6.40 ng L⁻¹ (average of 3.93 ± 1.15 ng L⁻¹) and from 1.52 to 21.2 ng g⁻¹ (average of 6.60 ± 4.31 ng g⁻¹ dw), respectively. The distribution of CUOCPs in water was consistent throughout XJ, but that in sediments was not uniform, indicating a stronger impact of floods on water than on sediments. Water-sediment partition coefficients were generally >2 L g⁻¹, showing a tendency of CUOCP dominance in sediments. The results of principal component analysis and cluster analysis showed that the occurrence of CUOCPs is significantly affected by exogenous disturbance, which could be flood events; meanwhile, clusters of CUOCPs were found in both water and sediments in the source-limited middle reaches in urban areas. Redundancy analysis (RDA) showed that CUOCP occurrences were not positively correlated with nutrient elements (nitrogen and phosphorus), but related to pH and dissolved oxygen (DO), indicating complex sources.

Numerous toxicological studies have shown that ingestion of pesticides can induce physiological stress in breeding birds, with adverse consequences on egg laying parameters and offspring quality through parental effects. However, previous studies do not mimic current levels of pesticide residues in typical landscapes, and they do not consider potential cocktail effects of pesticides as they occur in the wild. Herein, we explored whether realistic pesticide exposure affected reproduction parameters and offspring condition through parental effects in Grey partridge. We fed 24 breeding pairs with either seeds from conventional agriculture crops treated with various pesticides during cropping, or organic grains without pesticide residues as controls. The conventional and organic grain diets mimicked food options potentially encountered by wild birds in the field. The results showed that ingesting low pesticide doses over a long period had consequences on reproduction and offspring quality without altering mortality in parents or chicks. Compared with organic pairs, conventional pairs yielded smaller chicks at hatching that had a lower body mass index at 24 days old. Additionally, these chicks displayed lower haematocrit when body mass index was higher. Therefore, ingestion of conventional grains by parents resulted in chronic exposure to pesticide residues, even at low doses, and this had detrimental consequences on offspring. These results demonstrate a sublethal effect of pesticide residues through parental effects. The consequences of parental exposure on chicks might partly explain the decline in wild Grey partridge populations, which raises questions for avian conservation and demography if current agrosystem approaches are continued.

The endocrine-disrupting effects of human exposure to polybrominated diphenyl ethers (PBDEs) have been studied, but their associations with menstrual status were not clearly clarified. This study was to evaluate the associations between PBDE levels in adipose tissues and the menstrual cycle and menstrual bleeding duration alteration. A total of 298 female cases undergoing surgery were recruited from two hospitals in Shantou, China. Demographic, clinical, and pathological information were collected, and adipose tissues were obtained during mammary or abdominal surgery. Gas chromatography with mass spectrometry was used to analyze 14 PBDE congeners in the adipose samples. The associations between PBDE levels and menstrual cycle (MC) and menstrual duration (MD) were analyzed by logistic regression models, estimating odds ratios (ORs) and 95% confidence intervals (CIs). BDE-153 had the highest level in the adipose samples, followed by BDE-209, BDE-183 and BDE-47. Compared with referents, median levels of BDE-47, -71, −153, −183 were higher in women with MC > 30 days (all P < 0.05); BDE-47, -153, −183, −209 were also higher in women with MD > 5.5 days (all P < 0.05). After adjusted for age and parity, BDE-47, -71, −153, −183 were associated with prolonged MC (OR = 1.20, 1.15, 1.12, 1.11, respectively, all P < 0.05) in the logistic regression models; and BDE-47, -153, −183, −209 were associated with the prolonged MD (OR = 1.13, 1.09, 1.10, 1.11, respectively, all P < 0.05). Several individual PBDE congeners in female adipose were found associated with prolonged menstrual cycle and menstrual duration. PBDEs may influence reproductive health of women by altering menstrual status.

Tetracycline (TC) and Mn(II) is a common antibiotic and metal ion respectively. Nevertheless, literatures involving in the effects of Mn(II) on TC transformation are still insufficient. In this study, the kinetic experiment, spectral analysis, complexation experiment and electrochemical analysis, theoretical calculation and products detection were carried out to probe into oxidation and photolysis of TC with Mn(II). Mn(II) greatly accelerated TC oxidation, preferably tending to complex with TC at O10 – O12 or O2 – O3 site. There were a TC-Mn(II)/TC-Mn(III) redox couple and electron transfer process. Conversely, Mn(II) inhibited photolysis of TC. The photolysis of excited TC could compete with energy dissipation reactions. The electron transfer and complexation reaction easily made excited TC energy transfer, thus slowing down photolysis process. During the TC transformation, the intensity of functional groups was significantly decreased. Simultaneously, the degradation pathways mainly included eight reactions. It is a very interesting and probably overlooked phenomenon, which identifies new transformation of TC with Mn(II). This study helps to further understand fate and environmental behavior of antibiotics and metal ion.

Estuarine environments faced with contaminations from coastal zones and the inland are vital sinks of antibiotic resistance genes (ARGs). However, little is known about the temporal-spatial pattern of ARGs and its predominant constraints in estuarine environments. Here, we leveraged metagenomics to investigate ARG profiles from 16 China's estuaries across 6 climate zones in dry and wet seasons, and disentangled their relationships with environmental constraints. Our results revealed that ARG abundance, richness, and diversity in dry season were higher than those in wet season, and ARG abundance exhibited an increasing trend with latitude. The prevalence of ARGs was significantly driven by human activities, mobile gene elements, microbial communities, antibiotic residuals, physicochemical properties, and climatic variables. Among which, climatic variables and human activities ranked the most important factors, contributing 44% and 36% of the total variance of observed ARGs, respectively. The most important climatic variable shaping ARGs is temperature, where increasing temperature is associated with decreased ARGs. Our results highlight that the prevalence of ARGs in estuarine environments would be co-driven by anthropogenic activities and climate, and suggest the dynamics of ARGs under future changing climate and socioeconomic development.

In recent years, Feammox has made it possible to remove NH₄⁺-N under anaerobic conditions; however, its application in practical wastewater treatment processes has not been extensively reported. In this study, an up-flow anaerobic biological filter based on limonite (Lim-UAF) was developed to facilitate long-term and stable treatment of domestic sewage. Lim-UAF achieved the highest removal efficiency of chemical oxygen demand (COD) and NH₄⁺-N at a hydraulic retention time (HRT) of 24 h (Stage II). Specifically, the COD and NH₄⁺-N content decreased from 240.8 and 30.0 mg/L to about 7.5 and 0.35 mg/L, respectively. To analyze the potential nitrogen removal mechanism, the Lim-UAF was divided into three layers according to the height of the reactor. The results showed that COD and NH₄⁺-N removal had remarkable characteristics in Lim-UAF. More than 55.0% of influent COD was removed in the lower layer (0–30 cm) of Lim-UAF, while 60.2% of NH₄⁺-N was removed in the middle layer (30–60 cm). Microbial community analysis showed that the community structure in the middle and upper layers (60–90 cm) was relatively similar, but quite different from that of the lower layer. Heterotrophic bacteria were dominant in the lower layer, whereas iron-reducing and iron-oxidizing bacteria were enriched in the upper and middle layers. The formation of secondary minerals (siderite and Fe(OH)₃) indicated that the Fe(III)/Fe(II) redox cycle occurred in Lim-UAF, which was triggered by the Feammox and NDFO processes. In summary, limonite was used to develop a single-stage wastewater treatment process for simultaneously removing organic matter and NH₄⁺-N, which has excellent application prospects in domestic sewage treatment.

Liver disease has become a growing health burden, and little is known about the impairment of liver function caused by exposure to organophosphate esters (OPEs) in adolescents aged 12–19 years in the United States. To investigate the relationship between urinary metabolites of OPEs including diphenyl phosphate (DPHP), bis(1,3-dichloro-2-propyl) phosphate (BDCPP), bis(1-chloroethyl) phosphate (BCPP), bis(2-chloroethyl) phosphate (BCEP), and dibutyl phosphate (DBUP) and liver function in US adolescents aged 12–19 years. Liver function tests (LFTs) include aspartate aminotransferase (AST), albumin (ALB), alanine aminotransferase (ALT), alkaline phosphatase (ALP), gamma-glutamyl transferase (GGT), total bilirubin (TBIL), total protein (TP), and AST/ALT. Meanwhile, potential confounding and interaction effects were assessed. The study sample included 592 adolescents aged 12–19 from two consecutive NHANES cycles (2011–2012, 2013–2014). A composite statistical strategy combining traditional linear regression with advanced multi-pollutant models quantile based g-computation (QGC) and eXtreme Gradient Boosting (XGBoost) regression was used to analyze the joint effects of multiple OPEs on liver function indicators, and to describe the interaction between different OPEs in detail. 592 adolescent participants were 15 (14–17) years old, with similar numbers of males and females (304 vs. 288). The analysis results showed that (1) in the linear regression model, individual DPHP, BCEP exposure and ALP changes, BCEP and AST/ALT changes were positively associated. DPHP, BDCPP were negatively associated with TP changes. (2) The combined effects of various OPEs on ALB, ALT, ALP, GGT, TBIL, TP, and AST/ALT were statistically significant. (3) There is no potential interaction between different OPEs. Several OPEs and their combinations are closely related to the 8 LFT indicators. In addition, data suggest that exposure to OPEs in adolescents may be associated with liver damage. Due to limited evidence in the literature and potential limitations of the current study, our findings require more studies to confirm.

Antibiotics are omnipresent in the environment due to their widespread use, and they have wide-ranging negative impacts on organisms. Virus resistance differs substantially between domesticated Apis mellifera and wild Apis cerana, although both are commonly raised in China. Here, we investigated whether antibiotics can increase the sensitivity of honey bees to viral infection using the Israeli acute paralysis virus (IAPV) and tetracycline as representative virus and antibiotic. Although IAPV multiplied to lower levels in A. cerana than A. mellifera, resulting in decreased mortality (P < 0.01), there was no significant difference in immune responses to viral infection between the two species. Adult worker bees (A. cerana and A. mellifera) were treated with or without tetracycline to demonstrate the prominent role of gut microbiota against viral infection, and found Lactobacillus played a vital antiviral role in A. cerana. In A. cerana but not A. mellifera, tetracycline treatment reduced clearly bee survival and increased susceptibility to IAPV infection (P < 0.01). Our findings revealed that long-term antibiotic treatment in A. mellifera had altered the native gut microbiome and promoted the sensitivity to viral infection. We highlight the effects of antibiotics exposure on resistance to microbial and viral infection.