A study of the impact of an abandoned lead (Pb) mine (“Las Musas”), located in SW Spain, on the contamination of the surface soil and pastures in its vicinity revealed the presence of widely distributed, high levels of Pb contamination. The total Pb concentrations in soils sampled at distances from 3 to 998 m from the mine ranged between 129 and 1053 mg/kg, when it has been reported that non-polluted soils have concentrations of 29–40 mg/kg. These exceed the maximum tolerable levels in agricultural soils for the protection of environmental and human health as established in international and regional regulations. While the concentrations of potentially bioavailable Pb in the soils also surpassed the regulatory levels, the effective bioavailable fractions were low. The Pb concentrations measured in native plants ranged from 1.70 to 129 mg/kg dry weight, with Cynosurus echinatus, Philadelphus coronarius, and Fraxinus angustifolia being the species that bioaccumulated the greatest concentrations of this metal. Estimation of the environmental risk to wildlife and livestock grazing in the studied area showed no potential toxicity for these animals.

Publicado en Environmental Science and Pollution Research, Volume 26, pages 30386–30398, (2019) con DOI https://doi.org/10.1007/s11356-019-06197-5 | A study of the impact of an abandoned lead (Pb) mine (“Las Musas”), located in SW Spain, on the contamination of the surface soil and pastures in its vicinity revealed the presence of widely distributed, high levels of Pb contamination. The total Pb concentrations in soils sampled at distances from 3 to 998 m from the mine ranged between 129 and 1053 mg/kg, when it has been reported that non-polluted soils have concentrations of 29–40 mg/kg. These exceed the maximum tolerable levels in agricultural soils for the protection of environmental and human health as established in international and regional regulations. While the concentrations of potentially bioavailable Pb in the soils also surpassed the regulatory levels, the effective bioavailable fractions were low. The Pb concentrations measured in native plants ranged from 1.70 to 129 mg/kg dry weight, with Cynosurus echinatus, Philadelphus coronarius, and Fraxinus angustifolia being the species that bioaccumulated the greatest concentrations of this metal. Estimation of the environmental risk to wildlife and livestock grazing in the studied area showed no potential toxicity for these animals. | peerReviewed

Over time, the consumption of pharmaceutical drugs has highly augmented, directly contributing for an increase of the discharges of these substances into sewage water due to excretion, and their direct release to the environment, with or without adequate treatment. Considering that part of the sewage water is dumped into rivers and seas, this is the major source of contamination of the aquatic environment. Paracetamol and acetylsalicylic acid are among the most worldwide consumed pharmaceutical drugs, frequently found in wastewater discharges and consequently in the aquatic environment in considerable amounts, posing ecotoxicity concerns especially towards aquatic non-target species. Thus, it is important to study the ecotoxicological implications that these drugs might pose to organisms from aquatic environments. The objective of this study was to assess the toxic effects of these two compounds on key biochemical features (antioxidant defenses and damage, metabolism, and cholinergic neurotoxicity) of the marine snail species Gibbula umbilicalis after an acute (96 h) exposure, simulating pulses of contamination. In order to understand the effects that those drugs have on this species, the biochemical biomarkers analyzed were the activities of catalase (CAT), glutathione-S-transferases (GSTs), cholinesterases (ChEs), and the levels of lipid peroxidation (TBARS). After acute exposure to paracetamol, catalase activity decreased significantly in organisms exposed to both highest concentrations; no significant alterations were observed for glutathione-S-transferases activity; TBARS concentration decreased significantly in organisms exposed to the intermediate and both highest concentrations, and cholinesterase activity increased significantly in animals exposed to the lowest concentration. However, after acute exposure to acetylsalicylic acid, catalase activity increased significantly; no significant alterations were observed for glutathione-S-transferases activity, and TBARS concentrations and cholinesterase activity increased. This set of data shows that G. umbilicalis is highly responsive to the presence of the tested drugs, and may thus be a promising species to serve as test organism in future marine ecotoxicological testing. The adoption of this species may broaden the offer of highly ecologically representative test organisms to be included in biomonitoring projects of the coastal and marine environment. Furthermore, it is possible to suggest that both drugs may pose significant deleterious effects of pro-oxidative origin to the physiology of the selected species, with potential adverse ecological consequences, even after short periods of exposure. The absence of neurotoxicity showed that despite being able to trigger antioxidant mechanisms, both drugs did not affect neurotransmission.

Over time, the consumption of pharmaceutical drugs has highly augmented, directly contributing for an increase of the discharges of these substances into sewage water due to excretion, and their direct release to the environment, with or without adequate treatment. Considering that part of the sewage water is dumped into rivers and seas, this is the major source of contamination of the aquatic environment. Paracetamol and acetylsalicylic acid are among the most worldwide consumed pharmaceutical drugs, frequently found in wastewater discharges and consequently in the aquatic environment in considerable amounts, posing ecotoxicity concerns especially towards aquatic non-target species. Thus, it is important to study the ecotoxicological implications that these drugs might pose to organisms from aquatic environments. The objective of this study was to assess the toxic effects of these two compounds on key biochemical features (antioxidant defenses and damage, metabolism, and cholinergic neurotoxicity) of the marine snail species Gibbula umbilicalis after an acute (96 h) exposure, simulating pulses of contamination. In order to understand the effects that those drugs have on this species, the biochemical biomarkers analyzed were the activities of catalase (CAT), glutathione-S-transferases (GSTs), cholinesterases (ChEs), and the levels of lipid peroxidation (TBARS). After acute exposure to paracetamol, catalase activity decreased significantly in organisms exposed to both highest concentrations; no significant alterations were observed for glutathione-S-transferases activity; TBARS concentration decreased significantly in organisms exposed to the intermediate and both highest concentrations, and cholinesterase activity increased significantly in animals exposed to the lowest concentration. However, after acute exposure to acetylsalicylic acid, catalase activity increased significantly; no significant alterations were observed for glutathione-S-transferases activity, and TBARS concentrations and cholinesterase activity increased. This set of data shows that G. umbilicalis is highly responsive to the presence of the tested drugs, and may thus be a promising species to serve as test organism in future marine ecotoxicological testing. The adoption of this species may broaden the offer of highly ecologically representative test organisms to be included in biomonitoring projects of the coastal and marine environment. Furthermore, it is possible to suggest that both drugs may pose significant deleterious effects of pro-oxidative origin to the physiology of the selected species, with potential adverse ecological consequences, even after short periods of exposure. The absence of neurotoxicity showed that despite being able to trigger antioxidant mechanisms, both drugs did not affect neurotransmission. | published

Source apportionment of atmospheric PM1 is important for air quality control, especially in urban areas where high mass concentrations are often observed. Chemical analysis of molecular inorganic and organic tracer compounds and subsequently data analysis with receptor models give insight on the origin of the PM₁ sources. In the present study, four source apportionment approaches were compared with an extended database containing inorganic and organic compounds that were measured during an intensive sampling campaign at urban traffic and urban background sites in Barcelona. Source apportionment of the combined database, containing both inorganic and organic compounds, was compared with more conventional approaches using inorganic and organic databases separately. Traffic emission sources were identified in all models for the two sites. The combined inorganic and organic databases provided higher discrimination capacity of emission sources. It identified aerosols generated by regional recirculation of biomass burning, secondary biogenic organic aerosols, harbor emissions, and specific industrial emissions. In this respect, this approach identified a relevant industrial source situated at NE Barcelona in which a waste incinerator plant, a combined-cycle power plant, and an industrial glass complex are located. Models using both inorganic and organic molecular tracer compounds improve the source apportionment of urban PM.

14 pages, 3 figures, 2 tables, supplementary material https://doi.org/10.1007/s11356-019-06199-3 | Source apportionment of atmospheric PM1 is important for air quality control, especially in urban areas where high mass concentrations are often observed. Chemical analysis of molecular inorganic and organic tracer compounds and subsequently data analysis with receptor models give insight on the origin of the PM1 sources. In the present study, four source apportionment approaches were compared with an extended database containing inorganic and organic compounds that were measured during an intensive sampling campaign at urban traffic and urban background sites in Barcelona. Source apportionment of the combined database, containing both inorganic and organic compounds, was compared with more conventional approaches using inorganic and organic databases separately. Traffic emission sources were identified in all models for the two sites. The combined inorganic and organic databases provided higher discrimination capacity of emission sources. It identified aerosols generated by regional recirculation of biomass burning, secondary biogenic organic aerosols, harbor emissions, and specific industrial emissions. In this respect, this approach identified a relevant industrial source situated at NE Barcelona in which a waste incinerator plant, a combined-cycle power plant, and an industrial glass complex are located. Models using both inorganic and organic molecular tracer compounds improve the source apportionment of urban PM | Financial support for this study was provided by the Marie Curie FP7 SAPUSS (FP7-PEOPLE-2009-IEF, project number 254773), research projects from the D.G. de Calidad y Evaluacion Ambiental (Spanish Ministry of the Environment), and the Plan Nacional de IyD (Spanish Ministry of Science and Innovation) CGL2010-19464-VAMOS and CGL2011-29621, and the Generalitat de Catalunya (AGAUR 2017 SGR41) | Peer Reviewed

Marine macroalgae are a promising source of diverse bioactive compounds with applications in the biocontrol of harmful cyanobacteria blooms (cyanoHABs). In this work, we evaluated the potential algicidal activities of 14 species of seaweed collected from the coast of Souiria Laqdima, Morocco. Methanol extracts were screened in solid and liquid medium against the growth of the toxic cyanobacteria Microcystis aeruginosa and the microalgae Chlorella sp. used as food supplement. The results in solid medium revealed that the algicidal activity was limited to M. aeruginosa with the extract of Bornetia secundiflora showing the highest growth inhibition activity against Microcystis (27.33 ± 0.33 mm), whereas the extracts of Laminaria digitata, Halopytis incurvus, Ulva lactuca, and Sargasum muticum showed no inhibition. In liquid medium, the results indicated that all methanolic extracts of different macroalgae tested have a significant inhibitory effect on M. aeruginosa compared with that of the negative control. The maximum inhibition rates of M. aeruginosa were produced by the extracts of Bifurcaria tuberculata, Codium elongatum, and B. secundiflora. Moreover, the extracts of B. secundiflora recorded the maximum inhibition rate of Chlorella sp. Overall, the results highlight the potential of the extracts from macroalgae to control toxic cyanobacteria species.

Marine macroalgae are a promising source of diverse bioactive compounds with applications in the biocontrol of harmful cyanobacteria blooms (cyanoHABs). In this work, we evaluated the potential algicidal activities of 14 species of seaweed collected from the coast of Souiria Laqdima, Morocco. Methanol extracts were screened in solid and liquid medium against the growth of the toxic cyanobacteria Microcystis aeruginosa and the microalgae Chlorella sp. used as food supplement. The results in solid medium revealed that the algicidal activity was limited to M. aeruginosa with the extract of Bornetia secundiflora showing the highest growth inhibition activity against Microcystis (27.33 ± 0.33 mm), whereas the extracts of Laminaria digitata, Halopytis incurvus, Ulva lactuca, and Sargasum muticum showed no inhibition. In liquid medium, the results indicated that all methanolic extracts of different macroalgae tested have a significant inhibitory effect on M. aeruginosa compared with that of the negative control. The maximum inhibition rates of M. aeruginosa were produced by the extracts of Bifurcaria tuberculata, Codium elongatum, and B. secundiflora. Moreover, the extracts of B. secundiflora recorded the maximum inhibition rate of Chlorella sp. Overall, the results highlight the potential of the extracts from macroalgae to control toxic cyanobacteria species. | We acknowledge the projects TOXICROP (823860) funded by the H2020 program MSCA-RISE-2018 and the project VALORMAR (24517) of the 10/SI/2016-I&DT Empresarial- Programas Mobilizadores funded by the European Regional Development Fund (ERDF) and by the European Social Fund (ESF).

Depending on the actual number of soil-based on-site wastewater treatment system (OWTS) in an area, on-site sanitation may be a significant source of pollutants and a threat to groundwater. Even in the case of a system functioning correctly, here, a sand filter substituted for the in-situ soil, as the treated effluent may reach to the water table, it is necessary evaluating in situ how much the sand and underneath soil respectively contribute to pollutant removal. On the plot of a household in a small rural community, the functioning of a real scale OWTS was monitored for 1.5 years. This system, composed of a septic tank connected to a 5 × 5 m² and 0.7-m thick aerobic sand filter was equipped with soil hydrodynamic probes (water content and matrix potential) during construction. By using the instantaneous profile method of water content, the intermittent infiltrated flux was determined across the sand-pack according to position and time. Treated water infiltrates into underneath soil acting as post-treatment. Quality of interstitial liquid from the sand and the soil was analysed each month on a 12-h pumping sample obtained through porous plates. Results of water fluxes and concentrations provide an estimate of the annual flux to the vadose zone and groundwater of metals, nutrients and some organic micro-pollutants (parabens and triclosan) through the OWTS and subsoil.

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