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Chemical and anatomical changes in Liquidambar styraciflua L. xylem after long term exposure to elevated CO2 Full text
2015
Kim, Keonhee | Labbé, Nicole | Warren, Jeffrey M. | Elder, Thomas | Rials, Timothy G.
The anatomical and chemical characteristics of sweetgum were studied after 11 years of elevated CO2 (544 ppm, ambient at 391 ppm) exposure. Anatomically, branch xylem cells were larger for elevated CO2 trees, and the cell wall thickness was thinner. Chemically, elevated CO2 exposure did not impact the structural components of the stem wood, but non-structural components were significantly affected. Principal component analysis (PCA) was employed to detect differences between the CO2 treatments by considering numerous structural and chemical variables, as well as tree size, and data from previously published sources (i.e., root biomass, production and turnover). The PCA results indicated a clear separation between trees exposed to ambient and elevated CO2 conditions. Correlation loadings plots of the PCA revealed that stem structural components, ash, Ca, Mg, total phenolics, root biomass, production and turnover were the major responses that contribute to the separation between the elevated and ambient CO2 treated trees.
Show more [+] Less [-]Fractionation and mobility of Cu, Fe, Mn, Pb and Zn in the road dust retained on noise barriers along expressway – A potential tool for determining the effects of driving conditions on speciation of emitted particulate metals Full text
2015
Świetlik, Ryszard | Trojanowska, Marzena | Strzelecka, Monika | Bocho-Janiszewska, Anita
Road dust (RD) retained on noise barriers was used as a monitor of emission of traffic-related metals from expressway. By using SEM/EDX analysis it has been revealed that the main components of this particulate were irregular fine aggregates and tire debris with a ragged porous structure and with inclusions derived from the road surface. The results of chemical fractionation showed that driving conditions influence strongly a distribution pattern of Cu, whereas the atmospheric corrosion process affects a distribution pattern of Zn. The distribution pattern of Cu originating only from vehicle braking emission was “isolated” from the distribution pattern of road traffic copper. The predicted comparative mobilities of the emitted metals form the order: Zn >> Cu ≈ Mn > Pb >> Fe. The high mobility of zinc (K = 0.61) may create a current inhalation hazard and may be a source of future environmental hazard in the areas adjacent to heavily trafficked roads.
Show more [+] Less [-]Proteomic profile in Perna viridis after exposed to Prorocentrum lima, a dinoflagellate producing DSP toxins Full text
2015
Huang, Lu | Zou, Ying | Weng, Hui-wen | Li, Hong-Ye | Liu, Jie-Sheng | Yang, Wei-Dong
In the current study, we compared protein profiles in gills of Perna viridis after exposure to Prorocentrum lima, a dinoflagellate producing DSP toxins, and identified the differential abundances of protein spots using 2D-electrophoresis. After exposure to P. lima, the level of okadaic acid (a main component of DSP toxins) in gills of P. viridis significantly increased at 6 h, but mussels were all apparently healthy without death. Among the 28 identified protein spots by MALDI TOF/TOF-MS, 12 proteins were up-regulated and 16 were down-regulated in the P. lima-exposed mussels. These identified proteins were involved in various biological activities, such as metabolism, cytoskeleton, signal transduction, response to oxidative stress and detoxification. Taken together, our results indicated that the presence of P. lima caused DSP toxins accumulation in mussel gill, and might consequently induce cytoskeletonal disorganization, oxidative stress, a dysfunction in metabolism and ubiquitination/proteasome activity.
Show more [+] Less [-]Antibiotic resistance marker genes as environmental pollutants in GMO-pristine agricultural soils in Austria Full text
2015
Woegerbauer, Markus | Zeinzinger, Josef | Gottsberger, Richard Alexander | Pascher, Kathrin | Hufnagl, Peter | Indra, Alexander | Fuchs, Reinhard | Hofrichter, Johannes | Kopacka, Ian | Korschineck, Irina | Schleicher, Corina | Schwarz, Michael | Steinwider, Johann | Springer, Burkhard | Allerberger, Franz | Nielsen, Kaare M. | Fuchs, Klemens
Antibiotic resistance genes may be considered as environmental pollutants if anthropogenic emission and manipulations increase their prevalence above usually occurring background levels. The prevalence of aph(3′)-IIa/nptII and aph(3′)-IIIa/nptIII – frequent marker genes in plant biotechnology conferring resistance to certain aminoglycosides – was determined in Austrian soils from 100 maize and potato fields not yet exposed to but eligible for GMO crop cultivation. Total soil DNA extracts were analysed by nptII/nptIII-specific TaqMan real time PCR. Of all fields 6% were positive for nptII (median: 150 copies/g soil; range: 31–856) and 85% for nptIII (1190 copies/g soil; 13–61600). The copy-number deduced prevalence of nptIII carriers was 14-fold higher compared to nptII. Of the cultivable kanamycin-resistant soil bacteria 1.8% (95% confidence interval: 0–3.3%) were positive for nptIII, none for nptII (0–0.8%). The nptII-load of the studied soils was low rendering nptII a typical candidate as environmental pollutant upon anthropogenic release into these ecosystems.
Show more [+] Less [-]International scale implementation of the CNOSSOS-EU road traffic noise prediction model for epidemiological studies Full text
2015
Morley, D.W. | de Hoogh, K. | Fecht, D. | Fabbri, F. | Bell, M. | Goodman, P.S. | Elliott, P. | Hodgson, S. | Hansell, A.L. | Gulliver, J.
The EU-FP7-funded BioSHaRE project is using individual-level data pooled from several national cohort studies in Europe to investigate the relationship of road traffic noise and health. The detailed input data (land cover and traffic characteristics) required for noise exposure modelling are not always available over whole countries while data that are comparable in spatial resolution between different countries is needed for harmonised exposure assessment. Here, we assess the feasibility using the CNOSSOS-EU road traffic noise prediction model with coarser input data in terms of model performance. Starting with a model using the highest resolution datasets, we progressively introduced lower resolution data over five further model runs and compared noise level estimates to measurements. We conclude that a low resolution noise model should provide adequate performance for exposure ranking (Spearman's rank = 0.75; p < 0.001), but with relatively large errors in predicted noise levels (RMSE = 4.46 dB(A)).
Show more [+] Less [-]Genetic variation of Lymnaea stagnalis tolerance to copper: A test of selection hypotheses and its relevance for ecological risk assessment Full text
2015
Côte, Jessica | Bouétard, Anthony | Pronost, Yannick | Besnard, Anne-Laure | Coke, Maïra | Piquet, Fabien | Caquet, Thierry | Coutellec, Marie-Agnès
Genetic variation of Lymnaea stagnalis tolerance to copper: A test of selection hypotheses and its relevance for ecological risk assessment Full text
2015
Côte, Jessica | Bouétard, Anthony | Pronost, Yannick | Besnard, Anne-Laure | Coke, Maïra | Piquet, Fabien | Caquet, Thierry | Coutellec, Marie-Agnès
The use of standardized monospecific testing to assess the ecological risk of chemicals implicitly relies on the strong assumption that intraspecific variation in sensitivity is negligible or irrelevant in this context. In this study, we investigated genetic variation in copper sensitivity of the freshwater snail Lymnaea stagnalis, using lineages stemming from eight natural populations or strains found to be genetically differentiated at neutral markers. Copper-induced mortality varied widely among populations, as did the estimated daily death rate and time to 50% mortality (LT50).Population genetic divergence in copper sensitivity was compared to neutral differentiation using the QST-FST approach. No evidence for homogenizing selection could be detected. This result demonstrates that species-level extrapolations from single population studies are highly unreliable. The study provides a simple example of how evolutionary principles could be incorporated into ecotoxicity testing in order to refine ecological risk assessment.
Show more [+] Less [-]Genetic variation of Lymnaea stagnalis tolerance to copper: A test of selection hypotheses and its relevance for ecological risk assessment | Genetic variation of Lymnaea stagnalis tolerance to copper: A test of selection hypotheses and its relevance for ecological risk assessment: Genetic variation of Lymnaea stagnalis tolerance to copper: A test of selection hypotheses and its relevance for ecological risk assessment Full text
2015
Côte, Jessica | Bouetard, Anthony | Pronost, Yannick | Besnard, Anne-Laure | Coke, Maïra | Piquet, F. | Caquet, Thierry | Coutellec, Marie-Agnès | Écologie et santé des écosystèmes (ESE) ; Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST | Unité d'Ecologie et Ecotoxicologie Aquatiques (UEEA) ; Institut National de la Recherche Agronomique (INRA) | Institut en Santé Agro-Environnement (ISAE) | Département Ecologie des Forêts, Prairies et milieux Aquatiques (DEPT EFPA) ; Institut National de la Recherche Agronomique (INRA)
INRA-Onema "Phylogeny and Polluosensitivity" program This work was supported by the INRA-Onema 2013-2014 "Phylogeny and Polluosensitivity" program. The authors thank Marc Collinet and Antoine Gallard for technical assistance. Elsevier sci ltd | International audience | The use of standardized monospecific testing to assess the ecological risk of chemicals implicitly relies on the strong assumption that intraspecific variation in sensitivity is negligible or irrelevant in this context. In this study, we investigated genetic variation in copper sensitivity of the freshwater snail Lymnaea stagnalis, using lineages stemming from eight natural populations or strains found to be genetically differentiated at neutral markers. Copper-induced mortality varied widely among populations, as did the estimated daily death rate and time to 50% mortality (LT50). Population genetic divergence in copper sensitivity was compared to neutral differentiation using the Qs-r-FsT approach. No evidence for homogenizing selection could be detected. This result demonstrates that species-level extrapolations from single population studies are highly unreliable. The study provides a simple example of how evolutionary principles could be incorporated into ecotoxicity testing in order to refine ecological risk assessment. (C) 2015 Elsevier Ltd. All rights reserved.
Show more [+] Less [-]Influence of feeding and earthworm density on compound bioaccumulation in earthworms Eisenia andrei Full text
2015
Šmídová, Klára | Šerá, Jana | Bielská, Lucie | Hofman, Jakub
Earthworm density and feeding during exposure to contaminated soil have been used inconsistently in bioaccumulation studies, which may lead to possible errors in risk assessment and modeling. Hydrophobic organic pollutants with a wide range of environmental properties (phenanthrene, pyrene, lindane, p,p′-DDT, and PCB 153) were used to study the effect of different earthworm densities in combination with the presence or absence of feeding on bioaccumulation factors (BAFs). Similar BAFs were found at various soil-to-worm ratios, with the exception of phenanthrene. We recommend using at least 15 gsoil dw per earthworm. The absence of feeding doubled the BAFs and, thus, using no food ration can be considered as “the worst case scenario”. Whenever food is to be applied (i.e. to ensure the validity of the test in earthworm mass loss), we suggest feeding depending on the organic carbon content of the studied soil.
Show more [+] Less [-]Quantifying the effect of vegetation on near-road air quality using brief campaigns Full text
2015
Tong, Zheming | Whitlow, Thomas H. | MacRae, Patrick F. | Landers, Andrew J. | Harada, Yoshiki
Many reports of trees' impacts on urban air quality neglect pattern and process at the landscape scale. Here, we describe brief campaigns to quantify the effect of trees on the dispersion of airborne particulates using high time resolution measurements along short transects away from roads. Campaigns near major highways in Queens, NY showed frequent, stochastic spikes in PM2.5. The polydisperse PM2.5 class poorly represented the behavior of discrete classes. A transect across a lawn with trees had fewer spikes in PM2.5 concentration but decreased more gradually than a transect crossing a treeless lawn. This coincided with decreased Turbulence Kinetic Energy downwind of trees, indicating recirculation, longer residence times and decreased dispersion. Simply planting trees can increase local pollution concentrations, which is a special concern if the intent is to protect vulnerable populations. Emphasizing deposition to leaf surfaces obscures the dominant impact of aerodynamics on local concentration.
Show more [+] Less [-]Sources, occurrence and predicted aquatic impact of legacy and contemporary pesticides in streams Full text
2015
McKnight, Ursula S. | Rasmussen, Jes J. | Kronvang, Brian | Binning, Philip J. | Bjerg, Poul L.
We couple current findings of pesticides in surface and groundwater to the history of pesticide usage, focusing on the potential contribution of legacy pesticides to the predicted ecotoxicological impact on benthic macroinvertebrates in headwater streams. Results suggest that groundwater, in addition to precipitation and surface runoff, is an important source of pesticides (particularly legacy herbicides) entering surface water. In addition to current-use active ingredients, legacy pesticides, metabolites and impurities are important for explaining the estimated total toxicity attributable to pesticides. Sediment-bound insecticides were identified as the primary source for predicted ecotoxicity. Our results support recent studies indicating that highly sorbing chemicals contribute and even drive impacts on aquatic ecosystems. They further indicate that groundwater contaminated by legacy and contemporary pesticides may impact adjoining streams. Stream observations of soluble and sediment-bound pesticides are valuable for understanding the long-term fate of pesticides in aquifers, and should be included in stream monitoring programs.
Show more [+] Less [-]Health risk assessment of various metal(loid)s via multiple exposure pathways on children living near a typical lead-acid battery plant, China Full text
2015
Cao, Suzhen | Duan, Xiaoli | Zhao, Xiuge | Wang, Beibei | Ma, Jin | Fan, Delong | Sun, Chengye | He, Bin | Wei, Fusheng | Jiang, Guibin
Manufacture of lead-acid batteries is of widespread interest because of its emissions of heavy metals and metalloids into environment, harming environmental quality and consequently causing detrimental effects on human health. In this study, exposure pathways and health risks of children to heavy metal(loid)s (Pb, Cd, As, etc) were investigated based on field sampling and questionnaire. Pb was one of the most abundant elements in children's blood, with an elevated blood lead level of 12.45 μg dL−1. Soil/dust and food were heavily polluted by targeted metal(loid)s. Food ingestion accounted for more than 80% of the total exposure for most metal(loid)s. The non-cancer risks to children were 3–10 times higher than the acceptable level of 1, while the cancer risks were 5–200 times higher than the maximum acceptable level of 1.0 × 10−4. The study emphasized the significance of effective environmental management, particularly to ensure food security near battery facilities.
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