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A multibiomarker approach on the Atlantic tomcod (Microgadus tomcod) in the St. Lawrence Estuary Full text
2013
Dupuy, Celie | Couillard, Catherine M. | Laroche, Jean | Nellis, Pierre | Brousseau, Pauline | Fournier, Michel
A multibiomarker approach was developed on juvenile Atlantic tomcod (Microgadus tomcod) to evaluate the pertinence of this approach for low-cost screening assessment of the environmental quality of various coastal sites within estuaries. Several biometric indices and biomarkers (ethoxyresorufin-O-deethylase (EROD) activity, metallothionein concentration, and immune responses) were investigated on immature and maturing tomcods (a parts per thousand currency sign31 months) collected in four environmentally contrasted sites in the St. Lawrence Estuary (SLE). Simultaneous examination of various age classes provides the opportunity to detect short-term responses in sensitive young-of-the-year fish (e.g., EROD induction) and longer-time effects associated with chronic exposure and bioaccumulation (e.g., metallothionein induction). Principal component analysis was helpful to discriminate between responses possibly related to contaminant exposure (EROD, metallothionein) and responses that could be affected by upstream-downstream gradient (immune response, biometric indices). Measurement of a battery of biomarkers in young tomcods at several sites along the shore of the SLE is a low-cost screening investigation useful to identify hot spots requiring further investigation with chemical analysis and additional reference sites.
Show more [+] Less [-]Integrative leaf-level phytotoxic ozone dose assessment for forest risk modelling Full text
2013
Dizengremel, Pierre | Jolivet, Yves | Tuzet, Andree | Ranieri, A. | Le Thiec, Didier | Ecologie et Ecophysiologie Forestières [devient SILVA en 2018] (EEF) ; Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL) | Environnement et Grandes Cultures (EGC) ; Institut National de la Recherche Agronomique (INRA)-AgroParisTech | University of Pisa [Italy] = Università di Pisa [Italia] = Université de Pise [Italie] (UniPi) | Absent | R. Matyssek. | N. Clarke | P. Cudlin | T.N. Mikkelsen | J.P. Tuovinen | G. Wieser | E. Paoletti
Vol.13 (Krupa, S. ed.) Chap. 13 | absent
Show more [+] Less [-]Histopathological lesions and DNA adducts in the liver of European flounder (Platichthys flesus) collected in the Seine estuary versus two reference estuarine systems on the French Atlantic coast Full text
2013
Cachot, Jerome | Cherel, Yan | Larcher, Thibaut | Pfohl-leszkowicz, Annie | Laroche, Jean | Quiniou, Louis | Morin, Jocelyne | Schmitz, Julien | Burgeot, Thierry | Pottier, Didier
Histopathological lesions and DNA adducts in the liver of European flounder (Platichthys flesus) collected in the Seine estuary versus two reference estuarine systems on the French Atlantic coast Full text
2013
Cachot, Jerome | Cherel, Yan | Larcher, Thibaut | Pfohl-leszkowicz, Annie | Laroche, Jean | Quiniou, Louis | Morin, Jocelyne | Schmitz, Julien | Burgeot, Thierry | Pottier, Didier
An epidemiological survey was conducted in the Seine estuary and in two smaller and relatively preserved estuaries on the French Atlantic coast in order to estimate the occurrence of liver lesions in European flounder, Platichthys flesus, and also to seek putative risk factors for the recorded pathologies. Four hundred and seventy-eight fish of both sexes and of different size ranges were sampled in the three studied areas, 338 of which in the Seine estuary. All fish were examined for histopathological liver lesions, while DNA adducts and otoliths were analyzed on a subsample. Five categories of hepatic lesions were recorded with the following prevalence for the Seine estuary: 36.7 % inflammations, 8 % parasites (mainly encysted nematodes), 6.5 % foci of cellular alteration (FCA), 5.3 % foci of necrosis or regeneration (FNR), and 1.5 % tumors. Inflammation occurrence increased according to age, contrary to parasitic infestations and FCA which were more prevalent in young fish, notably those of < 1 year old (group 0). Tumors were only observed in females of more than two winters. Females exhibited a higher prevalence of tumors (3.0 %) and FCA (6.5 %) than males (0 and 2.6 %, respectively). Parasitic and infectious lesions and FNR were equally distributed in males and females. The prevalence of FNR was also shown to vary according to sampling season, with significantly more occurrences of liver necrosis in the fish collected in summer than in spring. Spatial differences were observed with a higher occurrence of encysted parasites in flounders from the upper Seine estuary, while inflammations predominated in flounders living downstream. Temporal trends were also noted, with an increased prevalence of parasitic infestations, inflammations, and FCA in the 2002-2003 period in comparison to the 1996-1997 one. The three flounder populations from the Seine estuary (Normandy), Ster estuary (Brittany), and Bay of Veys (Normandy) showed different spectra of hepatic lesions. Flounders from the Bay of Veys had relatively few liver lesions as compared to flounders from the two other estuaries. Flounders from the Ster estuary exhibited the highest prevalence of parasites (37.2 %) and inflammations (51.1 %). Finally, FCA and liver tumors occurred at very similar levels in both flounder populations from the Seine and the Ster estuaries. Group 0 flounders inhabiting the upper Seine estuary were more prone to parasitic and pre-neoplastic hepatic lesions and had higher levels of liver DNA adducts than the older ones living downstream. It was postulated that group 0 European flounders may serve as valuable bioindicators for assessing the quality of estuarine waters and the health status of euryhaline fish populations.
Show more [+] Less [-]Histopathological lesions and DNA adducts in the liver of European flounder (Platichthys flesus) collected in the Seine estuary versus two reference estuarine systems on the French Atlantic coast Full text
2013
Cachot, Jérôme | Cherel, Yan | Larcher, Thibaut | Pfohl-Leszkowicz, Annie | Laroche, Jean | Quiniou, Louis | Morin, Jocelyne | Schmitz, Julien | Burgeot, Thierry | Pottier, Didier
An epidemiological survey was conducted in the Seine estuary and in two smaller and relatively preserved estuaries on the French Atlantic coast in order to estimate the occurrence of liver lesions in European flounder, Platichthys flesus, and also to seek putative risk factors for the recorded pathologies. Four hundred and seventy-eight fish of both sexes and of different size ranges were sampled in the three studied areas, 338 of which in the Seine estuary. All fish were examined for histopathological liver lesions, while DNA adducts and otoliths were analyzed on a subsample. Five categories of hepatic lesions were recorded with the following prevalence for the Seine estuary: 36.7 % inflammations, 8 % parasites (mainly encysted nematodes), 6.5 % foci of cellular alteration (FCA), 5.3 % foci of necrosis or regeneration (FNR), and 1.5 % tumors. Inflammation occurrence increased according to age, contrary to parasitic infestations and FCA which were more prevalent in young fish, notably those of <1 year old (group 0). Tumors were only observed in females of more than two winters. Females exhibited a higher prevalence of tumors (3.0 %) and FCA (6.5 %) than males (0 and 2.6 %, respectively). Parasitic and infectious lesions and FNR were equally distributed in males and females. The prevalence of FNR was also shown to vary according to sampling season, with significantly more occurrences of liver necrosis in the fish collected in summer than in spring. Spatial differences were observed with a higher occurrence of encysted parasites in flounders from the upper Seine estuary, while inflammations predominated in flounders living downstream. Temporal trends were also noted, with an increased prevalence of parasitic infestations, inflammations, and FCA in the 2002–2003 period in comparison to the 1996–1997 one. The three flounder populations from the Seine estuary (Normandy), Ster estuary (Brittany), and Bay of Veys (Normandy) showed different spectra of hepatic lesions. Flounders from the Bay of Veys had relatively few liver lesions as compared to flounders from the two other estuaries. Flounders from the Ster estuary exhibited the highest prevalence of parasites (37.2 %) and inflammations (51.1 %). Finally, FCA and liver tumors occurred at very similar levels in both flounder populations from the Seine and the Ster estuaries. Group 0 flounders inhabiting the upper Seine estuary were more prone to parasitic and pre-neoplastic hepatic lesions and had higher levels of liver DNA adducts than the older ones living downstream. It was postulated that group 0 European flounders may serve as valuable bioindicators for assessing the quality of estuarine waters and the health status of euryhaline fish populations.
Show more [+] Less [-]Histopathological lesions and DNA adducts in the liver of European flounder (<em>Platichthys flesus</em>) collected in the Seine estuary versus two reference estuarine systems on the French Atlantic coast Full text
2013
Cachot, Jerome | Cherel, Yan | Larcher, Thibaut, | Pfohl-Leszkowicz, Annie | Laroche, Jean | Quiniou, Louis | Morin, Jocelyne | Schmitz, Julien | Burgeot, Thierry | Pottier, Didier
An epidemiological survey was conducted in the Seine estuary and in two smaller and relatively preserved estuaries on the French Atlantic coast in order to estimate the occurrence of liver lesions in European flounder, Platichthys flesus, and also to seek putative risk factors for the recorded pathologies. Four hundred and seventy-eight fish of both sexes and of different size ranges were sampled in the three studied areas, 338 of which in the Seine estuary. All fish were examined for histopathological liver lesions, while DNA adducts and otoliths were analyzed on a subsample. Five categories of hepatic lesions were recorded with the following prevalence for the Seine estuary: 36.7 % inflammations, 8 % parasites (mainly encysted nematodes), 6.5 % foci of cellular alteration (FCA), 5.3 % foci of necrosis or regeneration (FNR), and 1.5 % tumors. Inflammation occurrence increased according to age, contrary to parasitic infestations and FCA which were more prevalent in young fish, notably those of < 1 year old (group 0). Tumors were only observed in females of more than two winters. Females exhibited a higher prevalence of tumors (3.0 %) and FCA (6.5 %) than males (0 and 2.6 %, respectively). Parasitic and infectious lesions and FNR were equally distributed in males and females. The prevalence of FNR was also shown to vary according to sampling season, with significantly more occurrences of liver necrosis in the fish collected in summer than in spring. Spatial differences were observed with a higher occurrence of encysted parasites in flounders from the upper Seine estuary, while inflammations predominated in flounders living downstream. Temporal trends were also noted, with an increased prevalence of parasitic infestations, inflammations, and FCA in the 2002-2003 period in comparison to the 1996-1997 one. The three flounder populations from the Seine estuary (Normandy), Ster estuary (Brittany), and Bay of Veys (Normandy) showed different spectra of hepatic lesions. Flounders from the Bay of Veys had relatively few liver lesions as compared to flounders from the two other estuaries. Flounders from the Ster estuary exhibited the highest prevalence of parasites (37.2 %) and inflammations (51.1 %). Finally, FCA and liver tumors occurred at very similar levels in both flounder populations from the Seine and the Ster estuaries. Group 0 flounders inhabiting the upper Seine estuary were more prone to parasitic and pre-neoplastic hepatic lesions and had higher levels of liver DNA adducts than the older ones living downstream. It was postulated that group 0 European flounders may serve as valuable bioindicators for assessing the quality of estuarine waters and the health status of euryhaline fish populations.
Show more [+] Less [-]Analysis of the bacterial community in the two typical intertidal sediments of Bohai Bay, China by pyrosequencing Full text
2013
Wang, Liping | Liu, Lusan | Zheng, Binghui | Zhu, Yanzhong | Wang, Xing
For full understanding of the bacterial community in the intertidal zones of Bohai Bay, China, we used pyrosequencing-based approach to analyze the 16S rRNA gene of bacteria in the sediments from the two typically intertidal zones – Qikou (Qi) and Gaoshaling (Ga). Results showed that, at a 0.03 distance, the sequences from the Qi sediment were assigned to 3252 operational taxonomic units (OTUs) which belong to 34 phyla, 69 classes and 119 genera, while the 3740 OTUs from the Ga sediment were affiliated with 33 phyla, 66 classes and 146 genera. Comparing the bacterial communities inhabiting in the two intertidal sediments, we observed significant difference in the dominant composition and distribution at phylum, class and genus levels. Canonical correspondence analysis (CCA) showed that the median grain size and DO were the most important factors regulating the bacterial abundance and diversity, while the other environmental factors have effects with different degree.
Show more [+] Less [-]Contrasting key roles of Ruppia cirrhosa in a southern Mediterranean lagoon: Reservoir for both biodiversity and harmful species and indicator of lagoon health status Full text
2013
Dhib, Amel | Ben Brahim, Mounir | Turki, Souad | Aleya, Lotfi
The distribution of Ruppia cirrhosa meadow density and its epiphytic organisms in relation with environmental factors were studied in summer 2011 at five stations in the Ghar El Melh lagoon (GML; southern Mediterranean Sea). Eleven epiphytic groups were recognised among which diatoms and dinoflagellates were the dominant groups and greatest contributors to temporal dissimilarity. An overwhelming concentrations of harmful microalgae was recorded, mainly represented by the toxic dinoflagellate Prorocentrum lima with maximal concentrations attaining 6×105cells 100g−1 of Ruppia fresh weight. The epifauna community accounted for only 1.4% of total epiphyte abundance and was comprised predominantly of nematodes (47.51%), ciliates (32.59%), fish eggs (7.2%) and larvae (4.95%). PERMANOVA analyses revealed a significant spatio-temporal variation of all epiphytic groups (p<0.01). In this study, R. cirrhosa and its epiphytes were studied as potential early warning indicators of the health status of GML waters.
Show more [+] Less [-]Levels and spatial distribution of perfluorinated compounds (PFCs) in superficial sediments from the marine reserves of the Tuscan Archipelago National Park (Italy) Full text
2013
Perra, Guido | Focardi, Silvano Ettore | Guerranti, Cristiana
Surface sediments from marine reserves of the Tuscan Archipelago National Park (Italy), including remote islands not directly affected by anthropogenic influences, were analyzed for two dominant perfluorinated compounds (PFCs), namely perfluorooctane sulfonate (PFOS) and perfluorooctanoate (PFOA), using LC–ESI-MS/MS. The concentrations of PFOS were low, with medians ranging from 0.25 to 1.50ng/g dry wt, whereas PFOA was always below the detection limit of 0.1ng/g dry wt. The present paper can be considered a baseline study useful in future environmental monitoring programs.
Show more [+] Less [-]Temperature–salinity modeling for Ruwais coastal area in United Arab Emirates Full text
2013
Elshorbagy, Walid | Azam, Mir Hammadul | Elhakeem, Abubaker
A three dimensional rectangular grid model is applied to resolve the temperature–salinity dynamics of Ruwais, a segment of the UAE coast which is well known as dense water formation zone. The model employs a heat flux module and a turbulence closure scheme that facilitate realistic calculation of temperature–salinity dynamics. A field survey campaign is carried out to support the modeling study, involving measurements of tide, currents, temperature, and salinity. Investigation is done for two meteorologically extreme conditions, i.e. summer and winter. The model study showed that the western flux develops an anticlockwise circulation in the study area. The water industrial discharges elevated the temperature and salinity of the water near the southeastern shoreline. This water mass propagated towards north under the influence of gravity.
Show more [+] Less [-]Biomagnification of persistent chlorinated and brominated contaminants in food web components of the Yellow Sea Full text
2013
Byun, Gyo-Hyuk | Moon, Hyo-Bang | Ch'oe, Chŏng-hwa | Hwang, Jeomshik | Kang, Chang-Keun
Concentrations of polychlorinated biphenyls (PCBs), organochlorine pesticides (OCPs), and polybrominated diphenyl ethers (PBDEs) were measured in 32 species inhabiting the Yellow Sea to assess their bioaccumulation potentials. The concentrations in these samples were lower than those reported for other countries or locations. Relatively high levels of BDE 209 in biota suggest an ongoing source of deca-BDE technical mixing within the Yellow Sea. The accumulation profiles of PCBs were uniform between species, but the concentrations of OCPs and PBDEs varied widely. Pelagic and benthic food-chain components were separated by their δ13C values. Significant positive correlations between δ15N and PCB 153, PCB 138, p,p′-DDE, oxy-chlordane, and trans-nonachlordane were found only for pelagic consumers, indicating that the pelagic food chain is an important bioaccumulation pathway for selected PCB and OCP compounds. The other compounds did not show any biomagnification through benthic and pelagic food chains, suggesting the lower bioaccumulation potentials of these contaminants.
Show more [+] Less [-]Health impact assessment of marine emissions in Pearl River Delta region Full text
2013
Lai, H.K. | Tsang, H. | Chau, J. | Lee, C.H. | McGhee, S.M. | Hedley, A.J. | Wong, C.M.
Global marine vessels emissions are adversely affecting human health particularly in southeast Asia. But health burdens from both ocean- and river-going vessels in Pearl River Delta (PRD) regions are not quantified. We estimated the potential health impacts using pooled relative risks of mortality and hospital admissions in China, and the model derived concentrations of sulfur dioxide (SO2), particulate matter (PM10), nitrogen dioxide (NO2) and ozone (O3) due to vessels emissions. SO2 concentrations due to marine emissions in Hong Kong were 13.6μgm−3 compared with 0.7μgm−3 in PRD regions that were far from the marine vessels. In PRD regions, the estimated annual numbers (per million people) of excess deaths from all natural causes and hospital admissions from cardiorespiratory causes attributable to SO2, NO2, O3 and PM10 combined from marine emissions were 45 and 265 respectively. Marine emission control measures could contribute a large reduction in mortality and hospital admissions in PRD regions especially in Hong Kong.
Show more [+] Less [-]Link between sewage-derived nitrogen pollution and coral disease severity in Guam Full text
2013
Redding, Jamey E. | Myers-Miller, Roxanna L. | Baker, David M. | Fogel, Marilyn | Raymundo, Laurie J. | Kim, Kiho
The goals of this study were to evaluate the contribution of sewage-derived N to reef flat communities in Guam and to assess the impact of N inputs on coral disease. We used stable isotope analysis of macroalgae and a soft coral, sampled bimonthly, as a proxy for N dynamics, and surveyed Porites spp., a dominant coral taxon on Guam’s reefs, for white syndrome disease severity. Results showed a strong influence of sewage-derived N in nearshore waters, with δ15N values varying as a function of species sampled, site, and sampling date. Increases in sewage-derived N correlated significantly with increases in the severity of disease among Porites spp., with δ15N values accounting for more than 48% of the variation in changes in disease severity. The anticipated military realignment and related population increase in Guam are expected to lead to increased white syndrome infections and other coral diseases.
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