The increased frequency and intensity of short-term extreme warming phenomena have been associated to harsh biological and ecosystem outcomes (i.e., mass mortalities in marine organisms). Marine heatwaves (MHWs), occurring when seasonal temperature threshold is exceeded for at least 5 consecutive days, may reduce the tolerance of coastal species toward additional pressures, but interactions between such multiple stressors are virtually unexplored. The present study aimed to characterize in Mytilus galloprovincialis the influence of a simulated MHW scenario on the toxicological effects of the pharmaceutical carbamazepine (CBZ), ubiquitously detected in the marine environment and chosen as model compound for this relevant class of emerging contaminants. The bioaccumulation of CBZ and responsiveness of various biological parameters, including immune system, antioxidant status, lipid metabolism and cellular integrity, were analyzed in exposed mussels both during and after the end of the heatwave. MHW appeared to strongly modulate accumulation of CBZ, paralleled by weakened immunocompetence and onset of oxidative disturbance that finally evolved to cellular damages and lipid metabolism disorders. Elaboration of the overall results through a quantitative Weight of Evidence model, revealed the highest hazard in organisms exposed to both the stressors 10 days after the end of the heatwave, suggesting that MHWs could leave a footprint on the capability of mussels to counteract CBZ toxicity, thus affecting their vulnerability and predisposition to adverse effects toward multiple stressors.

Intermediate volatility organic compounds (IVOCs) have great potential to form secondary organic aerosols (SOA) in the atmosphere. Thus, a high-resolution IVOC emission inventory is essential for the accurate simulation of SOA formation. This study developed the first nationwide on-road vehicular IVOC emission inventory in China based on localized measurement of the IVOC emission factors and volatility distributions for various vehicle types. The total vehicular IVOC emissions in China in 2019 were estimated to be 241.2 Gg. Heavy-duty trucks, light-duty trucks, and light-duty passenger vehicles contributed the most, accounting for 47.6%, 24.6%, and 16.9% of total vehicular IVOC emissions, respectively. Although much higher in number, gasoline vehicles contributed 15.0%, which was far less than the contribution of diesel vehicles. The two peaks in volatility bins B₁₂–B₁₃ and B₁₆–B₁₇ accounted for 42.2% and 23.7% of the total IVOC emissions, respectively. By gridding the emission inventory into a relatively high resolution of 0.1° × 0.1°, high-emission areas and hotspots were clearly identified. In general, eastern China had substantially higher vehicular IVOC emissions than western China. High-emission areas with emission intensity >10 Mg·grid⁻¹ covered most of the North China Plain, Yangtze River Delta, and Pearl River Delta. The emission intensity over the downtown areas of Beijing and Shanghai exceeded 50 Mg·grid⁻¹. In contrast, IVOC emissions over western China were relatively lower, with a network structure gathering around the traffic arteries serving inter-provincial transportation. This study underscored the importance of having a localized emission factor to better reflect the IVOC emission characteristics from Chinese vehicles and to improve the assessment of their environmental impacts.

Aquaculture ecosystem is a hot-spot for antibiotic resistance genes (ARGs). Rice-crayfish co-culture was considered an eco-friendly aquaculture model and has been widely adopted in China. However, it is unclear whether rice-crayfish co-culture is one of the most eco-friendly models from the perspective of antibiotic resistance profiles. In this study, we evaluated the eco-friendliness of rice-crayfish co-culture, and compared this model with other aquaculture models, from the perspectives of antibiotics and ARG patterns, based on multi-omics and antibiotic profiles. Results showed that the nutrient levels, antibiotic concentrations, dominant microbial genera and ARG patterns in the rice-crayfish co-culture model were profoundly different from the other three aquaculture models (crab only aquaculture model, crayfish only aquaculture model, and crab-crayfish co-culture models). Specifically, the rice-crayfish co-culture model has significantly lower diversity of ARGs and lower potential risks of ARGs when compared to the other aquaculture models. Nutrient and antibiotic concentrations were the important environmental factors for shaping ARG patterns, but compared with environmental factors, the effects of mobile genes and bacteria community on the proliferation and transmission of ARGs were stronger. This study has deepened our understanding of ARGs in freshwater aquaculture ecosystem, and suggested that rice-crayfish co-culture model is a relatively eco-friendly aquaculture model when compared with the other aquaculture models.

The overuse of pesticides for augmenting agriculture productivity always comes at the cost of environment, biodiversity, and human health and has put the land, water, and environmental footprints under severe threat throughout the globe. Underpinning and maximizing the microbiome functions in pesticide-contaminated environments has become a prerequisite for a sustainable environment and resilient agriculture. It is imperative to elucidate the metabolic network of the microbial communities and environmental variables at the contaminated site to predict the best strategy for remediation and soil microbe-pesticide interactions. High throughput next-generation sequencing and in silico analysis allow us to identify and discern the members and characteristics of core microbiomes at the contaminated site. Integration of modern high throughput multi-omics investigations and informatics pipelines provide novel approaches and pathways to capitalize on the core microbiomes for enhancing environmental functioning and mitigation. The role of eco-genomics tools in visualising the microbial network, taxonomy, functional potential, and environmental variables in contaminated habitats is discussed in this review. The integrated role of the potential microbe identification as individual or consortia, mechanistic approach for pesticide degradation, identification of responsible enzymes/genes, and in silico approach is emphasized for the prospects of the area.

Toxicokinetics may help assessing the risk of metal-contaminated soils by quantifying the development of internal metal concentrations in organisms over time. This study assessed the toxicokinetics in Enchytraeus crypticus of non-essential (Pb and Cd) and essential elements (Zn and Cu) in metal-contaminated field soils from a mining area, containing 3.49–24.3 mg Cd/kg dry soil, 433–1416 mg Pb/kg dry soil, 15.7–44.9 mg Cu/kg dry soil and 1718–6050 mg Zn/kg dry soil. Three different uptake-elimination patterns in E. crypticus were found. Both essential elements (Zn and Cu) showed fast increasing internal concentrations reaching equilibrium within 2 d in the uptake phase, without hardly any elimination after transfer to clean soil. The non-essential Cd showed a slow linear accumulation and excretion with body concentrations not reaching steady state within 21 d. Internal Pb concentrations, however, reached equilibrium within 7 d in the uptake phase. Longer exposure times in ecotoxicological tests, therefore, are required for elements like Cd. Porewater pH and dissolved organic carbon (DOC) levels were the dominant factors controlling Cd uptake from the test soils. The 21-d body Cd and Pb concentrations were best explained from 0.01 M CaCl₂-extractable soil concentrations. Steady-state Cu and Zn body concentrations were independent of soil exposure concentrations. Bioaccumulation factors (BAF) were low for Pb (<0.1 kgₛₒᵢₗ/kgwₒᵣₘ), but high for Cd at 1.78–24.3 kgₛₒᵢₗ/kgwₒᵣₘ, suggesting a potential risk of Cd biomagnification in the terrestrial food chain of the mining area ecosystem.

As one of the most common ways to get rid of municipal waste, landfill leachate, waste with complicated compositions and high levels of contaminants, has become a significant threat to the world's environment. Here, the impact of sewage sludge (SS) and derived-biochar (SSB) amendments on the immobilization and potential mobility of heavy metals in a contaminated soil-plant system was investigated. The sequential fractionation findings showed that using SS-2%, SSB-2%, and SSBC-1% reduced the potential mobility of heavy metals while increasing the residual fraction in polluted soils. The translocation and bioconcentration factors showed that heavy metals were slightly transferred into shoots from roots and lowered accumulation in roots from contaminated soils. Fourier transform infrared (FTIR) and X-ray photoelectron spectrum (XPS) comprehensive characterization results indicated the significant role of applied amendments for heavy metals transformation from the exchangeable-soluble fractions to the least available form by lowering their mobility to confirm the adsorption-based complexes, which results in the surface adsorption of heavy metals with functional groups. The electron paramagnetic resonance (EPR) results indicated the dominance of reactive oxygen species (ROS) in the intracellular formation of hydroxyl radicals (•OH) in maize plant roots and shoots. ROS (•OH) generation plays a critical influence in the interaction between the physiological processes of plants and heavy metals. Moreover, all the amendments increased maize growth and biomass production. Our study suggests that alone and combined application of SS and SSB have great potential to remediate heavy metals contaminated soil for environmental sustainability.

The link between zinc exposure and glucose metabolism or the development of type 2 diabetes (T2D) is controversial, and underlying mechanisms are unclear. This study aimed to explore the associations of zinc exposure with glucose-insulin homeostasis traits and the long-term effects of zinc on the development of T2D, and further to estimate the potential roles of inflammation and oxidative damage in such relationships. We investigated 3890 urban adults from the Wuhan-Zhuhai cohort, and followed up every three years. Mixed linear model was applied to estimate dose-response associations between urinary zinc and glycemia traits [fasting plasma insulin (FPI), fasting plasma glucose (FPG), insulin resistance (homeostasis model assessment of insulin resistance, HOMA-IR), and β-cell dysfunction (homeostasis model assessment of β-cell function, HOMA-B)], as well as zinc and biomarkers for systemic inflammation (C-reactive protein) and oxidative damage (8-isoprostane and 8-hydroxy-2′-deoxyguanosine). Logistic regression model and Cox regression model were conducted to evaluate the relationships between urinary zinc and prevalence and incidence of T2D, respectively. We further performed mediation analysis to assess the roles of inflammation and oxidative damage biomarkers in above associations. At baseline, we observed significant dose-response relationships of elevated urinary zinc with increased FPI, FPG, HOMA-IR, and T2D prevalence and decreased HOMA-B, and such associations could be strengthened by increased C-reactive protein, 8-isoprostane, and 8-hydroxy-2′-deoxyguanosine. Elevated C-reactive protein significantly mediated 9.09% and 17.67% of the zinc-related FPG and HOMA-IR increments, respectively. In longitudinal analysis, a significantly positive association between urinary zinc and T2D incidence was observed among subjects with persistent high urinary zinc levels when compared with those with persistent low zinc levels. Our results suggested that high levels of zinc exposure adversely affected on glucose-insulin homeostasis and further contributed to increased risk of T2D cross-sectionally and longitudinally. Moreover, inflammatory response might play an important role in zinc-related glucose metabolic disorder.

Dichlorophenol (DCP), a commonly used fungicide and insecticide, is widely found in waters and wastewaters. Herein, the degradation of DCP by Ferrate (Fe(VI)) in different matrices was comprehensively investigated. In pure water, a complete removal of DCP was achieved in 300 s at [Fe(VI)]:[DCP] molar ratio of 2:1. The presence of HA (10 mg L⁻¹) inhibited DCP degradation to a certain extent. A total of twenty degradation products were identified by HPLC/MS analysis. Based on these products, reaction pathways including the cleavage of C–C bridge bond, hydroxylation, and radical coupling were proposed. These reaction mechanisms were further rationalized by theoretical calculations. The analyses of Wiberg bond orders and transition state indicated that C₇–C₈ bond was the most vulnerable site for cleavage, and C₁₂ site was the most likely site for hydroxyl addition. Mulliken atomic spin densities distribution suggested that self-coupling products was easily generated via C–O–C coupling ways. Finally, the feasibility of applying Fe(VI) to degrade DCP (20 μM) in a municipal wastewater effluent and a lake water was evaluated and verified. The findings in this study are of relevance in designing Fe(VI)-based treatment strategy for chlorine-containing persistent pesticides.

India struggles with frequent exceedances of the ambient air quality standard for particulate matter and benzene. In the past two decades, India has made considerable progress in tackling indoor air pollution, by phasing out kerosene lamps, and pushing biofuel using households towards Liquefied Petroleum Gas (LPG) usage. In this study, we use updated emission inventories and trends in residential fuel consumption, to explore changes in the contribution of different sectors towards India's largest air pollution problem. We find that residential fuel usage is still the largest air pollution source, and that the <10% households using cow dung as cooking fuel contribute ∼50% of the residential PM₂.₅ emissions. However, if current trends persist, residential biofuel usage in India is likely to be phased out by 2035. India's renewable energy policies are likely to reduce emissions in the heat and electricity sector, and manufacturing industries, in the mid-term. PM₂.₅ emissions from open waste burning, on the other hand, hardly changed in the decade from 2010 to 2020. We conclude that without strong policies to promote recycling and upcycling of non-biodegradable waste, and the conversion of biodegradable waste to biogas, open waste burning is likely to become India's largest source of air pollution by 2035. While our study is limited to India, our findings are of relevance for other countries in the global South suffering from similar waste management challenges.

Multiple systematic reviews and meta-analyses linked prenatal exposure to ambient air pollutants to adverse birth outcomes with mixed findings, including results indicating positive, negative, and null associations across the pregnancy periods. The objective of this study was to systematically summarise systematic reviews and meta-analyses on air pollutants and birth outcomes to assess the overall epidemiological evidence. Systematic reviews with/without meta-analyses on the association between air pollutants (NO₂, CO, O₃, SO₂, PM₂.₅, and PM₁₀) and birth outcomes (preterm birth; stillbirth; spontaneous abortion; birth weight; low birth weight, LBW; small-for-gestational-age) up to March 30, 2022 were included. We searched PubMed, CINAHL, Scopus, Medline, Embase, and the Web of Science Core Collection, systematic reviews repositories, grey literature databases, internet search engines, and references of included studies. The consistency in the directions of the effect estimates was classified as more consistent positive or negative, less consistent positive or negative, unclear, and consistently null. Next, the confidence in the direction was rated as either convincing, probable, limited-suggestive, or limited non-conclusive evidence. Final synthesis included 36 systematic reviews (21 with and 15 without meta-analyses) that contained 295 distinct primary studies. PM₂.₅ showed more consistent positive associations than other pollutants. The positive exposure-outcome associations based on the entire pregnancy period were more consistent than trimester-specific exposure averages. For whole pregnancy exposure, a more consistent positive association was found for PM₂.₅ and birth weight reductions, particulate matter and spontaneous abortion, and SO₂ and LBW. Other exposure-outcome associations mostly showed less consistent positive associations and few unclear directions of associations. Almost all associations showed probable evidence. The available evidence indicates plausible causal effects of criteria air pollutants on birth outcomes. To strengthen the evidence, more high-quality studies are required, particularly from understudied settings, such as low-and-middle-income countries. However, the current evidence may warrant the adoption of the precautionary principle.