International audience | Agricultural practices are a major cause of the current loss of biodiversity. Among postwar agricultural intensification practices, the use of plant protection products (PPPs) might be one of the prominent drivers of the loss of wildlife diversity in agroecosystems. A collective scientific assessment was performed upon the request of the French Ministries responsible for the Environment, for Agriculture and for Research to review the impacts of PPPs on biodiversity and ecosystem services based on the scientific literature. While the effects of legacy banned PPPs on ecosystems and the underlying mechanisms are well documented, the impacts of current use pesticides (CUPs) on biodiversity have rarely been reviewed. Here, we provide an overview of the available knowledge related to the impacts of PPPs, including biopesticides, on terrestrial vertebrates (i.e. herptiles, birds including raptors, bats and small and large mammals). We focused essentially on CUPs and on endpoints at the subindividual, individual, population and community levels, which ultimately linked with effects on biodiversity. We address both direct toxic effects and indirect effects related to ecological processes and review the existing knowledge about wildlife exposure to PPPs. The effects of PPPs on ecological functions and ecosystem services are discussed, as are the aggravating or mitigating factors. Finally, a synthesis of knowns and unknowns is provided, and we identify priorities to fill gaps in knowledge and perspectives for research and wildlife conservation.

International audience | Cadmium (Cd), which is naturally present in soils, is transferred to durum wheat grains at levels that have worrying consequences for food safety. There have been some reports that adding sulfur (S) to plants reduces Cd accumulation in shoots by increasing its retention in roots due to complexation of Cd by S-containing ligands. Consequently, low S availability in some agricultural soils could favor Cd contamination of durum wheat grains. We conducted a greenhouse experiment to test whether S deficiency would reduce Cd retention in roots, thus favoring its allocation to shoots. Durum wheat was grown in a nutrient solution containing a low level of Cd (100 nM) with sufficient (+ S) or deficient (-S) sulfate levels and harvested at heading. Surprisingly, in the -S treatment, more Cd was retained in roots and less allocated to shoots than in the + S treatment, and the same applied to P, K, Ca, Mg, Cu, Mn, Mo, and Zn. The reduced aboveground biomass resulting from S deficiency explained both the reduced net uptake (i.e., the total quantities of elements in the plant) and the reduced allocation of elements to shoots. Depending on the relative effects of S deficiency on shoot biomass compared to the effects of a reduction in S uptake and allocation to shoots, the reduced supply of S increased the concentrations of Cd and of P, K, Mn, Mo, and Zn in the shoot, whereas the concentration of Cu decreased, and that of Ca and Mg remained unchanged. The higher concentration of Cd in the shoots of S-deficient plants suggests that attention should be paid to the consequences of insufficient S for Cd contamination of durum wheat.

International audience | In the last two decades, awareness grew on the matter of the impact of environment on human health. Contaminants sorbed onto soil and settled dust can be ingested and thus represent a hazard, particularly to young children, who play on the ground and bring their hands and objects to their mouth. Metal(loid)s and polycyclic aromatic hydrocarbons (PAHs) are of concern as they are both carcinogenic to humans and ubiquitous in outdoor environments. The present study aims to assess the total and bioaccessible fractions of PAHs and metal(loid)s present in settled dust of four preschools located in industrial, urban, and suburban areas. On the one hand, children’s incremental life cancer risks (ILCR) were calculated according to ingestion pathway. On the other hand, the genotoxicities of the bioaccessible dust-bonded contaminants were determined on gastric cells. PAH concentrations ranged from 50.9 to 2267.3 ng/g, and the bioaccessible fraction represented 10.7% of the total in average. Metal(loid) concentration ranged from 12,430 to 38,941 µg/g, and the mean bioaccessibility was of 40.1%. Cancer risk ranged from 2.8.105 to 8.6.105, indicating that there is a potential cancer risk for children linked to the ingestion of settled dust. The inorganic bioaccessible fraction induced little DNA (< 20%TailDNA) and chromosomal damages (30% increase in micronuclei), whereas the organic bioaccessible fraction induced higher DNA (17–63%TailDNA) and chromosomal damages (88% increase in micronuclei). Such experimental approach needs to be deepen, as a tool complementary to cancer risk calculation, since the latter only lays on a set of targeted contaminants with known toxicity values.

International audience | Soil and the threats it faces nowadays are often underestimated by citizens. This is rather due to a lack of awareness than to a lack of interest. In France, the surface covered by private gardens is not negligible, as it represents 2% of the territory, which is four times larger than the area of natural reserves in the country. Therefore, ensuring the protection and preservation of healthy soils in these areas is of great importance. Citizen science is a good way to raise awareness about scientific issues. Using a scientific and educational approach, the citizen science project “La clé du sol,” funded by the Fondation de France coordinated by the evertéa Foundation, aimed to raise home gardeners’ awareness of soil complexity and how to maintain healthy soil, as well as help them realize the possible consequences of their practices. For 2 years, fourteen gardeners participated in the project which involved theoretical and practical workshops where cultivation practices were listed, garden soils were sampled, and their physico-chemical and biological parameters as well as contaminants were analyzed. The strength of this project was the close interaction between the gardeners, a local citizen association, agronomy students, and researchers to decipher and interpret the results and learn how to translate them into practical advice. The progress of the project is presented in this article. Moreover, the scientific, social, and educational contributions of “La clé du sol” as well as the challenges inherent in citizen science projects involving long-term citizen engagement will be discussed.

International audience | The increasing demand for electric vehicles (EVs) in Europe, coupled with legislative efforts to reduce combustion engine vehicles, has significantly spurred the manufacturing of lithium-ion batteries (LIBs). However, this growth has led to a rapid rise in EV-LIB scrap, from both retired batteries and manufacturing processes, a factor insufficiently addressed in prior research. Our study tackles this issue by assessing the harmonisation of industry projects and examining the recycling facilities' readiness to handle these dual waste streams. We methodically estimate the registrations of new EVs and their anticipated scrap volume, and then project future LIB manufacturing scrap in the EU. Therefore, we assess current and future recycling capacities and evaluate the balance between scrap influx and recycling readiness by 2030. Our findings indicate that the EU is facing a significant recycling challenge. By 2030, about 930 kilotonnes of scrap from the EV-LIB industry will need recycling under the baseline scenario. Although current capacities can handle today's scrap volumes, the disparity between rapidly expanding manufacturing and lagging recycling facilities will result in a shortfall in a few years. Current plans suggest EU recycling facilities will have a capacity of about 785 kilotonnes annually by 2030, which falls short of the dual scrap streams. This research underscores the need for a strategic approach to scale up recycling infrastructure and technology, to emphasise the importance of responsible manufacturing, and to align with the EU's sustainability goals and the growing demands of the EV industry.

International audience | Surfactants are used for a variety of applications such as emulsifiers, solubilizers, or foaming agents. Their intensive production and use in pharmaceutical, cosmetic and agricultural products have resulted in their continuous discharge in the environment, especially via wastewaters. Surfactants have become a threat to living organisms as they interact with, and disrupt, cell membranes and macromolecules. Their effects have mainly been studied in aquatic species; however, terrestrial organisms are also threatened by these emerging contaminants. This study investigates the effects of two widely used nonionic surfactants, Tween-20 and Triton X-100, on key traits of larvae and adults of the fruit fly Drosophila melanogaster. We assessed the toxicity of the two surfactants on viability, development time, body size and food intake of the flies. The results revealed that both surfactants induced toxic effects on the drosophila flies leading to decreased viability, delayed development and lowered food consumption at the highest tested concentrations. Both surfactants proved to be toxic to flies, and, for all tested traits, Triton X-100 appeared more toxic than Tween-20. Our results might extend to other invertebrates. The widespread use of these substances, which then end up in the environment, should be regulated to mitigate their impacts on biodiversity and ecosystems.

International audience | Soil and the threats it faces nowadays are often underestimated by citizens. This is rather due to a lack of awareness than to a lack of interest. In France, the surface covered by private gardens is not negligible, as it represents 2% of the territory, which is four times larger than the area of natural reserves in the country. Therefore, ensuring the protection and preservation of healthy soils in these areas is of great importance. Citizen science is a good way to raise awareness about scientific issues. Using a scientific and educational approach, the citizen science project “La clé du sol,” funded by the Fondation de France coordinated by the evertéa Foundation, aimed to raise home gardeners’ awareness of soil complexity and how to maintain healthy soil, as well as help them realize the possible consequences of their practices. For 2 years, fourteen gardeners participated in the project which involved theoretical and practical workshops where cultivation practices were listed, garden soils were sampled, and their physico-chemical and biological parameters as well as contaminants were analyzed. The strength of this project was the close interaction between the gardeners, a local citizen association, agronomy students, and researchers to decipher and interpret the results and learn how to translate them into practical advice. The progress of the project is presented in this article. Moreover, the scientific, social, and educational contributions of “La clé du sol” as well as the challenges inherent in citizen science projects involving long-term citizen engagement will be discussed.

International audience | The increasing demand for electric vehicles (EVs) in Europe, coupled with legislative efforts to reduce combustion engine vehicles, has significantly spurred the manufacturing of lithium-ion batteries (LIBs). However, this growth has led to a rapid rise in EV-LIB scrap, from both retired batteries and manufacturing processes, a factor insufficiently addressed in prior research. Our study tackles this issue by assessing the harmonisation of industry projects and examining the recycling facilities' readiness to handle these dual waste streams. We methodically estimate the registrations of new EVs and their anticipated scrap volume, and then project future LIB manufacturing scrap in the EU. Therefore, we assess current and future recycling capacities and evaluate the balance between scrap influx and recycling readiness by 2030. Our findings indicate that the EU is facing a significant recycling challenge. By 2030, about 930 kilotonnes of scrap from the EV-LIB industry will need recycling under the baseline scenario. Although current capacities can handle today's scrap volumes, the disparity between rapidly expanding manufacturing and lagging recycling facilities will result in a shortfall in a few years. Current plans suggest EU recycling facilities will have a capacity of about 785 kilotonnes annually by 2030, which falls short of the dual scrap streams. This research underscores the need for a strategic approach to scale up recycling infrastructure and technology, to emphasise the importance of responsible manufacturing, and to align with the EU's sustainability goals and the growing demands of the EV industry.

International audience | Biocontrol solutions (macroorganisms, microorganisms, natural substances, semiochemicals) are presented as potential alternatives to conventional plant protection products (PPPs) because they are supposed to have lower impacts on ecosystems and human health. However, to ensure the sustainability of biocontrol solutions, it is necessary to document the unintended effects of their use. Thus, the objectives of this work were to review (1) the available biocontrol solutions and their regulation, (2) the contamination of the environment (soil, water, air) by biocontrol solutions, (3) the fate of biocontrol solutions in the environment, (4) their ecotoxicological impacts on biodiversity, and (5) the impacts of biocontrol solutions compared to those of conventional PPPs. Very few studies concern the presence of biocontrol solutions in the environment, their fate, and their impacts on biodiversity. The most important number of results were found for the organisms that have been used the longest, and most often from the angle of their interactions with other biocontrol agents. However, the use of living organisms (micro and macroorganisms) in biocontrol brings a specific dimension compared to conventional PPPs because they can survive, multiply, move and colonize other environments. The questioning of regulation stems from this specific dimension of the use of living organisms. Concerning natural substances, the few existing results indicate that while most of them have low ecotoxicity, others have a toxicity equivalent to or greater than that of the conventional PPPs. There are almost no result regarding semiochemicals. Knowledge of the unintended effects of biocontrol solutions has proved to be very incomplete. Research remains necessary to ensure their sustainability.