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Lethal and sublethal effects of the saline stressor sodium chloride on Chironomus xanthus and Girardia tigrina Full text
2020
Dornelas, Aline Silvestre Pereira | Sarmento, Renato Almeida | Cavallini, Grasiele Soares | da Silva Barbosa, Rone | Vieira, Mayane Marques | de Souza Saraiva, Althiéris | Bordalo, Maria D. | Soares, Amadeu M. V. M. | Pestana, João L. T.
Salinization in freshwaters is gradually increasing as a result of human activities and climatic changes. Higher salt content causes stress for freshwater organisms. Sodium chloride (NaCl) is among the most frequently occurring salts in freshwater ecosystems. The objective of the present study was to investigate the lethal and sublethal effects of NaCl on freshwater ecosystems, using as test organism the dipteran Chironomus xanthus and the planarian Girardia tigrina. Acute tests showed that C. xanthus was more sensitive (48-h LC₅₀ (median lethal concentration) of 2.97 g NaCl L⁻¹) than G. tigrina (48-h LC₅₀ of 7.77 g NaCl L⁻¹). C. xanthus larvae growth rate (larvae length and head capsule width) was significantly reduced under exposure to concentrations as low as 0.19 g L⁻¹ NaCl and higher. A delay in the emergence time (EmT₅₀) was also demonstrated for the same concentration. Sublethal NaCl effects in G. tigrina included feeding inhibition (LOEC (lowest observed effect concentration) of 0.4 g L⁻¹), reduced locomotion (LOEC = 0.2 g L⁻¹), and 24–48-h blastema regeneration (LOEC = 0.2 g L⁻¹ and 0.1 g L⁻¹, respectively). The results demonstrated the toxicity of NaCl to C. xanthus and G. tigrina including sublethal effects that can result in negative consequences for populations in natural freshwaters under salinization.
Show more [+] Less [-]Gallic acid affects blood-brain barrier permeability, behaviors, hippocampus local EEG, and brain oxidative stress in ischemic rats exposed to dusty particulate matter Full text
2020
Mirshekari Jahangiri, Hamzeh | Sarkaki, Alireza | Farbood, Yaghoob | Dianat, Mahin | Goudarzi, Gholamreza
Dust storms are environmental natural events that transport high concentrations of particulate matter (PM) in living spaces. Recent epidemiological studies have shown that air pollution is associated with stroke. In the present study we aimed to investigate the probable protective effects of gallic acid (GA) on blood-brain barrier (BBB) disruption, brain oxidative stress, anxiety, depression, locomotion behaviors, and changes of hippocampal local electroencephalogram (local EEG) power induced by 4-vessel transient occlusion (4VO I/R) following exposure to dusty PM in rats. Male Wistar rats were divided randomly into eight groups: (1) vehicle+Sham (Veh + Sh), (2) vehicle+4VO I/R (Veh + I/R), (3) gallic acid+sham (GA + Sh), (4) gallic acid+4VO I/R (GA + I/R), (5) vehicle+PM (Veh + PM), (6) PM + 4VO I/R (PM + I/R), (7) gallic acid+PM + Sham (GA + PM + Sh), and (8) gallic acid+PM + 4Vo I/R (GA + PM + I/R). 4VO type of I/R was induced after 10 days of pretreatment by GA 100 mg/kg/2 ml/ip, or 2 ml/kg/ip, normal saline as vehicle (Veh) and exposure to dust storm composed of dusty PM (DPM, 2000–8000 μg/m3), 60 min daily for consecutive 10 days) simultaneously. Seventy-two hours after I/R induction, all behavioral tests and BBB permeability evaluation were done. Hippocampus local EEG was recorded just before and 72 h after I/R induction, and finally brain tissue oxidative stress was assayed. Data showed that 4VO I/R following exposure to DPM increased BBB permeability (p < 0.001), brain oxidative stress (p < 0.001), induced anxiety (p < 0.001), depression (p < 0.01), locomotion impairment (p < 0.001), superoxide dismutase (SOD) activity, and local EEG power also were decreased in PM + Sh (p < 0.001). Pretreatment with GA reversed BBB permeability and MDA. Our findings suggest that GA is a probable protective agent against adverse effects of both I/R and exposure to DPM and also in I/R subjects exposed to DPM. The beneficial effects of GA may induce by its antioxidative and anti-inflammatory properties.
Show more [+] Less [-]Multiple environmental factors analysis of flash flood risk in Upper Hanjiang River, southern China Full text
2020
Zhong, Ming | Jiang, Tao | Li, Kun | Lu, Qiaoqian | Wang, Jiao | Zhu, Jiajun
Identifying the environmental factors and analyzing the causal mechanism of flash floods help to manage the risk. Maximum 24-h precipitation (MP), digital elevation (DE), slope degree (SD), soil type (ST), drainage density (DD), and vegetation cover (VC) are selected as the risk factors of flash floods in this study. Precipitation is the important meteorological components in flash floods; thus spatial characteristics of precipitation trend have been analyzed by using Mann-Kendall tests, and a positive trend of precipitation in Upper Hanjiang River is detected. Then, association rule mining approach is proposed to investigate the multiple environmental factors of flash floods, in which both single and multiple dimension data mining have been conducted by Apriori algorithm. Considering the high rate of 5-year return period floods in the flash flood inventory, further association rule mining after sampling has been conducted in order to deeply mine the causal patterns of flash floods in different risk magnitudes. Results show that soil type, slope degree, and digital elevation are the dominant environmental factors of flash floods in the study area, and precipitation is one of the important causal factors in severe flash flood hazards. It is also highlighted that flash floods might easily occur even with a slight rainfall present due to the instability of sand clay and saturated soil moisture. The proposed novel use of field data and data mining has the potential for providing procedures and solutions for an effective interpretation of flash flood mechanism. The results are expected to be applicable for decision-making and sustainable management in flooding risk.
Show more [+] Less [-]Bone turnover markers in children and adolescents with environmentally determined short stature, living in the oil and gas processing region Full text
2020
Zhumalina, Akmaral K. | Tusupkaliev, Balash T. | Zharlikasinova, Mairamkul B. | Kim, Irina S. | Darzhanova, Klara B.
In conditions of intensive development of gas fields, environmental safety issues are becoming increasingly important. High rates of gas production and growth of its chemical processing have turned gas industry enterprises into a powerful source of environmental pollution, which poses a real threat to public health and contributes to an increase in morbidities and environmentally determined pathological conditions. The development of methods for the diagnosis, treatment and prevention of such conditions is an important task for modern science. The aim of the research is to make a comprehensive assessment of serum markers of bone synthesis and resorption in children with short stature living in the oil and gas processing regions. A total of 240 children aged 8–17 years, living in the oil and gas processing region (Kenkiyak), and 409 schoolchildren (8–17 years old), living in the environmentally favourable region (Kobda village), during 3 years were examined. It was established that emissions of pollutants by oil processing enterprises account for almost 80% of all emissions into the atmosphere. Gas flaring at oil production sites plays a special role in this process. Speaking of the effects of environmental factors on children’s health, it should be noted that there is no consensus on the mechanisms and consequences of exposure to low doses of chemical atmospheric pollutants in children and adolescents so far.
Show more [+] Less [-]Energy conservation and carbon reduction potential for solid waste in China Full text
2020
Wang, Minxi | Li, Zhi | Chen, Lu | Liu, Ping | Zhang, Rui | Liu, Wei | Ma, You | Wang, Yajie | Li, Xin
The generation of solid waste has increased dramatically in China, owing largely to the rapid development and expansion of the Chinese economy. The management of solid waste is critical and becoming a challenge for some cities in China. Waste recycling is an effective solution to solid waste management and seeks to balance ecological sustainability with economic improvements. This study assessed the energy conservation and CO₂ emission reduction potential of solid waste using an embodied energy/carbon model from a life cycle perspective. The results showed that compared with the production of virgin materials, solid waste recycling results in a reduction of 294.2 Mtce in energy consumption, and 614.5 Mt in CO₂ emission is shown in 2017. The recycling of steel waste was the highest contributor, accounting for more than 45% of energy conservation and at least 62% of CO₂ reduction. If 100% recycling of recyclable waste like steel waste and plastic waste can be achieved, energy conservation and CO₂ reduction could reach 551.89 Mtce and 933.69 Mt, respectively, accounting for 12.29% of energy consumption and 8.46% of CO₂ emission. A robust recycling system must be established to achieve the purpose of environment production and resource conservation. The proposed evaluation framework could help in the decision-making process. The waste classification must be promoted to increase waste recovery rate and improve waste reproduction technology to maximize energy conservation and CO₂ emission reduction.
Show more [+] Less [-]Eco-friendly pulping of wheat straw using crude xylano-pectinolytic concoction for manufacturing good quality paper Full text
2020
Varghese, Libin Mathew | Agrawal, Sharad | Nagpal, Raksha | Miśra, Omaprakāśa | Bhardwaj, Nishi Kant | Mahajan, Ritu
In this study, suitability of xylano-pectinolytic enzymes in pulping of wheat straw has been explored. The suitable biopulping conditions were optimized, with xylanase dose of 400 and pectinase dose of 120 IU/g wheat straw, 1:10 (g/ml) material to liquid ratio, 55 °C temperature, 3 h treatment time, 0.75% Tween 80 and pH 8.5. Enzymatic pretreatment efficiently increased the pulpability of wheat straw, generated pulp with higher yield, lower kappa number (15.67%) and rejections (59.65%) in comparison with chemical pulp. The brightness of pretreated wheat straw pulp with enzyme was 16.04% higher than that of the non-enzyme treated wheat straw pulp. The biopulping resulted in 12% reduction of pulping chemicals along with more residual alkali content, in order to achieve similar optical and chemical properties as obtained by 100% chemically treated pulp. Physical properties of pulp also improved after enzymatic pretreatment, increasing burst index (26.50%), tear index (18.22%) and breaking length (5.56%). The enzyme plus chemical (88% pulping chemicals) treated pulp showed improvement in brightness and whiteness, with reduction in yellowness at all bleaching stages. In comparison with chemically bleached pulp, biopulp with reduced alkali dose (88%) had higher breaking length (6.63%), double fold number (51.28%), tear index (2.83%), burst index (24.31%), along with increased viscosity (6.12%) and Gurley porosity (27.50%). These results clearly suggest that biopulping of wheat straw with xylano-pectinolytic enzymes can reduce chemical loading during soda-anthraquinone pulping and also improve the quality of paper. This is the first report demonstrating the biopulping of wheat straw using crude xylano-pectinolytic enzymes.
Show more [+] Less [-]The impact of age-related sub-chronic exposure to chlorpyrifos on metabolic indexes in male rats Full text
2020
Samarghandian, Saeed | Foadoddin, Moshen | Zardast, Mahmoud | Mehrpour, Omid | Sadighara, Parisa | Roshanravan, Babak | Farkhondeh, Tahereh
Chlorpyrifos (CPF), an organophosphorus pesticide (OP), has been implicated in metabolic diseases; however, the data are controversial. Rising age has been found as a main risk factor for metabolic diseases, and it has been proposed that advanced age increases susceptibility to the toxic effects of OPs. Therefore, this investigation aimed to evaluate the impact of CPF on hyperglycemia, hypercholesterolemia, and inflammation in animals with different ages. CPF (5 mg/kg) for 45 consecutive days was administered orally to male Wistar rats with different ages including 2-, 10-, and 20-month-old. The results indicated an increase in glucose and inflammatory indices, and also lipid profile was changed in the serum of aged animals in comparison with the 2-month-old animals. CPF administration amplified these parameters in the 20-month-old rats in comparison with that of aged-matched controls. The histopathological examination also indicated that CPF caused slight to moderate changes in the liver of 2-, 10-, and 20-month-old animals. Cholestasis was also observed in the CPF-administrated 20-month-old rats. In conclusion, aging may increase the susceptibility to CPF-induced metabolic disturbances in the animal models. It is proposed that advancing in age elevates the susceptibility to the metabolic effects of CPF.
Show more [+] Less [-]Ecotoxicological assessments of biochar additions to soil employing earthworm species Eisenia fetida and Lumbricus terrestris Full text
2020
Elliston, Tom | Oliver, Ian W.
Biochar is the degradation-resistant product generated by the pyrolysis of organic materials and is produced for the intended use of land application in order to promote carbon sequestration and soil improvement. However, despite the many potential benefits biochar application offers, it is important to quantify any ecological impacts that may result from soil amendment in order to avoid potentially causing negative effects upon soil biota which are crucial in the many ecosystem services provided by soil. Any impacts on earthworms in particular are important to evaluate because of their pivotal role in organic matter breakdown, nutrient cycling and soil formation. In this study, we conducted a series of ecotoxicological assays to determine lethal and sublethal (avoidance, mass change and moisture content) effects of heavy biochar applications that reflect levels that may be used in soil restoration efforts. Two earthworm species, Eisenia fetida, an epigeic species, and Lumbricus terrestris, an anecic species, were utilised as test organisms. Two types of biochar, produced from wheat straw and rice husk feedstocks, respectively, were applied to OECD artificial soil and to a natural soil (Kettering loam) at rates of up to 20% w/w. The influence of biochar application on soil porewater chloride, fluoride and phosphate concentrations was also assessed. The biochar applications induced only a subtle level of avoidance behaviour while effects on survival over a 4-week exposure period were inconsistent. However, death and physical damage to some individual earthworms at high biochar application rates were observed, the mechanisms and processes leading to which should be investigated further. Earthworm development (mean mass change over time) proved to be a more sensitive measure, revealing negative effects on L. terrestris at 10% and 20% (w/w) wheat biochar applications in OECD soil and at 20% (w/w) applications of both biochars in Kettering loam. The moisture content of E. fetida remained remarkably consistent across all treatments (~ 82%), indicating that this is not a sensitive measure of effects. The high rates of biochar application resulted in increased chloride (2 to 3-fold) and phosphate (100-fold) concentrations in simulated soil porewaters, which has important implications for soil fertility and production but also for environmental management.
Show more [+] Less [-]Application of biochar, compost and ZVI nanoparticles for the remediation of As, Cu, Pb and Zn polluted soil Full text
2020
Baragaño, Diego | Forján, Rubén | Fernández, Begoña | Ayala, Julia | Afif, Elias | Gallego, José Luis R.
Here we tested the capacity of zero valent iron nanoparticles (nZVI) combined with two organic amendments, namely, compost and biochar, to immobilize metal(oid)s such as As, Cu, Pb, and Zn. In addition, the effects of the amendments on the development of Brassica juncea L., a plant widely used for phytoremediation purposes, were also examined. To perform the experiments, pots containing polluted soil were treated with nZVI, compost-biochar, or a blend of compost-biochar-nZVI. Metal(oid)s availability and soil properties were evaluated after 15 and 75 days, and the height and weight of the plants were measured to determine development. The compost-biochar amendment showed excellent capacity to immobilize metals, but As availability was considerably increased. However, the addition of nZVI to the mixture corrected this effect considerably. In addition, soil treatment with nZVI alone led to a slight increase in Cu availability, which was not observed for the mixture with organic amendments. With respect to soil properties, the CEC and pH were enhanced by the compost-biochar amendment, thereby favoring plant growth. Nevertheless, the nanoparticles reduced the concentration of available P, which impaired plant growth to a certain extent. In conclusion, Fe-based nanoparticles combined with organic amendments emerge as powerful approaches to remediate soils contaminated by metals and metalloids.
Show more [+] Less [-]Effects of soil acid stress on the survival, growth, reproduction, antioxidant enzyme activities, and protein contents in earthworm (Eisenia fetida) Full text
2020
Wu, Jialong | Ren, Zongling | Zhang, Chi | Motelica-Heino, Mikael | Deng, Ting | Wang, Haoyu | Dai, Jun
This study focused on the study of earthworm survival, growth, reproduction, enzyme activities, and protein contents to evaluate and predict the effects of different soil pH levels and determine the optimal risk assessment indicators for the effects. Survival rate, growth rate, and cocoon number as well as four enzyme (glutathione peroxidase (GSH-PX), superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT)) activities and two proteins (total protein (TP) and metallothionein (MT)) contents in earthworms were determined to characterize the responses of earthworm activity to five soil pH levels. These biological datasets (survival, growth, and reproduction) were compared with biochemical indexes (GSH-PX, SOD, POD, CAT, TP, and MT), mainly using biphasic dose-response models. The results indicated that the soil pH value had significant inhibitory effects on the survival, growth, and reproduction of earthworms beginning with 3.0, 4.0, and 5.2, respectively. The dose-response models (J-shaped and inverted U-shaped curves) statistics indicated that the critical values (ECZEP) of the GSH-PX, SOD, POD, CAT, TP, and MT inhibited by soil acid stress were 3.46, 3.76, 3.35, 3.54, 3.50, and 3.96 (average 3.60), respectively. In the present study, the fitting curve analysis showed that the responses of the CAT activities and TP and MT contents in earthworm in response to soil pH have the behavior of hormesis.
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