Antibiotic resistance genes (ARGs) in rural environments have been poorly characterized in the literature. In this study, the diversity, abundance, and distribution of ARGs in surface waters, soils, and sediments of a typical hilly rural area in the Upper Yangtze River watershed were investigated using the high-throughput quantitative polymerase chain reaction, and their relationships with chemical properties of the samples were analyzed. No significant differences in the diversity and abundance of ARGs were observed among the three medium types while the ARG distribution pattern in the sediments was obviously different from that of the surface waters. According to the co-occurrence pattern of ARGs subtypes obtained by network analysis, blaOXA10-02, blaPSE, lnuB-02, and qacEΔ1-01 can be used to estimate the relative abundance of total ARGs for the study area. It appeared that the prevalence of ARGs in the sediments was promoted by the horizontal gene transfer (HGT) and vertical gene transfer together, while their spread in the surface waters and soils were facilitated by the supply of biogenic elements and HGT, respectively. Mobile genetic elements (MGEs) were abundant and detected in all samples, and their abundance was significantly and positively correlated with that of ARGs, implying that the potential horizontal transfer of ARGs to other bacteria and pathogens in rural environments should not be overlooked.

Based on a structural decomposition approach, the present paper simultaneously analyzes seven driving factors and forty sectors, which contribute to CO₂ emission changes for China’s transportation sector during 1992–2015. Based on the analysis of the driving factors, the total output and the energy intensity are found to be the primary positive and negative factor, respectively. From the sector analysis, top five sectors causing the increase of the transportation CO₂ emissions are identified. Particularly, information transmission, computer services and software sector (no. 28); construction sector (no. 26); transportation sector (no. 27); chemical sector (no. 12); and leasing and business service sector (no. 33) are the five most relevant contributions to the increase of CO₂ emissions of the transportation sector during 2002–2015. As a view to suppress CO₂ emission growth, besides the promotion of public transportation, low-carbon emission energy, and energy-saving technologies, policymakers should pay attention to the impacts of key sectors on transportation.

This study examines the role of industrialization in the energy-growth-FDI nexus for the case of South Africa using data over the period 1970 to 2018. The empirical exercise was conducted using Pesaran Autoregressive Distributed Lag (ARDL) bounds testing approach. To accomplish our study objective, we analyze stationarity properties of the series using the unit root test after which we applied Bayer-Hanck (B-H) combined technique to cointegration to assess whether a long-run relationship exists among the series. Empirical results show that a 1% change in FDI account for 0.002% and 0.013% increase in economic expansion in the short- and long- run respectively. Also, a 1% increase in coal consumption influence GDP negatively by 0.083% and 0.207% in the short and long run respectively. Furthermore, a 1% increase in total natural resource rent positively affects GDP by 0.02% and 0.05% respectively in the short and long run. Industrialization, on the other hand, demonstrates a positive and significant impact on the economic growth process both in the short and long run. Industrialization contributes 0.506% and 1.274% to economic expansion both in the short and long run respectively. The causality tests suggest that a one-way causal link running from FDI to industrialization and from industrialization to coal consumption exists. Finally, FDI inflow drives total natural resource rents in South Africa. This study also gives reliable growth and energy policy proposals to policymakers applicable to countries around the globe.

Water contamination by discharge of untreated or poorly treated wastewater into water bodies is a current issue that may cause harm to humans. Water quality assessment targets general parameters, which often misleads to underestimation of their implication in the environment. Acute and genotoxicity assays using Daphnia magna is a reliable tool for testing deleterious effects of wastewater exposure. This paper aimed at evaluating acute ecotoxicity as well as genotoxicity of a biological treatment system composed by an anaerobic bioreactor (AR), algal turf scrubber (ATS), followed by two downflow constructed wetlands (CW). The university’s wastewater treatment plant (WWTP) composed by an equalization tank (ET), an upflow anaerobic sludge blanket (UASB), followed by an aerobic bioreactor (AB) was also assessed for acute and genotoxicity. Our results showed the acute ecotoxicity ranged from moderately to extremely toxic, and from nontoxic to moderately toxic for ET and AB. For AR, most samples were moderately toxic. The outflow from ATS and CW’s tanks completely eliminated acute toxicity and genotoxicity evidencing that the proposed system combining ATS and vertical CWs is suitable for treating sanitary wastewater.

One of the biggest problems of modern world is the air pollution which causes the death of millions of people every year. The heavy metals included in the component of air pollution occupy an important position in human health since they could remain intact in nature for a long time and build bioaccumulation, and also some of them are toxic or carcinogenic even at some low concentrations. Even the heavy metals functioning as micronutrient element could create toxic effect for human beings at the high concentrations. For this reason, the determination of the heavy metal concentration has an important role in terms of the detection of risky regions and risky level. One of the primary sources of heavy metals is industrial plants where the heavy metal ores are processed. Those regions might have risks at high level in terms of particular heavy metals. Consequently, it is significant to find out in which level they influence the area around these plants and to what extent the heavy metal pollution is effective. In this study, Ba, Zn, Cd, K, and Na concentrations are determined by analyzing the samples which are taken from 1- and 2-year-old needles of Scots pines (Pinus sylvestris L.) growing in 1 km, 3 km, 10 km and 25 km distances around a processing and mining of magnesite ore in Russia. In the end of the study, it is concluded that generally, the concentrations of heavy metals subject to the study are increasing depending upon the distance, and this increase is quite apparent in some elements, and finally in many points, the concentrations determined in 2-year-old needles have higher levels than 1-year-old needles.

Imazalil (IMZ), a fungicide containing imidazole group, is extensively used for the prevention and treatment of fungal diseases in plants. Current study was performed to examine cyto-genotoxic potential of IMZ on Allium cepa roots by following Allium ana-telophase and single cell gel electrophoresis (comet) assays. The concentration which reduced the growth of the root tips of IMZ by 50% compared to the negative control group (EC₅₀) was found to be 1 μg/mL by Allium root growth inhibition test. 0.5, 1, and 2 μg/mL concentrations of IMZ were exposed to Allium roots for intervals of 24, 48, 72, and 96 h. 10 μg/mL of methyl methane sulfonate (MMS) and distilled water were used as control groups, both positive and negative. Statistical analysis was performed by using one-way ANOVA with Duncan’s multiple comparison tests at p ≤ 0.05 and Pearson correlation test at p = 0.01. IMZ showed cytotoxic effect by statistically decreasing root growth and mitotic index (MI) and also genotoxic effect by statistically increasing chromosomal aberrations (CAs) and DNA damage compared to the negative control group. With these cyto-genotoxic effects, it should be used carefully and further cyto-genotoxic mechanisms should be investigated along with other toxicity tests.

The study of subjective well-being (SWB) has attracted considerable attention from scholars globally. This has stimulated numerous studies that have identified regional and individual factors associated with SWB, but the extant research lacks multi-level studies that simultaneously examine their influence on SWB. Environmental pollution is one of such factors, but few studies have investigated its effect on SWB in China particularly. The current study addressed these problems by conducting hierarchical linear regressions to explore the effects of regional and individual factors on Chinese people’s SWB. Three major environmental pollutions (wastewater pollution, domestic waste pollution, and air pollution) were studied using data from the Chinese General Social Survey 2013 and China Statistical Yearbook 2014. The results indicated that wastewater pollution and domestic waste pollution had significant negative influence on SWB. Moreover, gross domestic product (GDP) per capita might contribute more to the improvement of SWB than income inequality. This implies that individuals’ SWB might be enhanced by improving absolute income, which is consistent with the micro-level proposition of the Easterlin paradox. Overall, these findings signal that effective management of environmental pollution is essential for promoting the SWB of the people in China.

Freshwater fish Cirrhinus mrigala were exposed to tris(2-chloroethyl) phosphate (TCEP) with three different concentrations (0.04, 0.2, and 1 mg/L) for a period of 21 days. During the study period, thyroid-stimulating hormone (TSH), triiodothyronine (T3), and thyroxine (T4) levels were significantly (p < 0.05) inhibited. The superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST), and lipid peroxidation (LPO) levels were increased significantly (p < 0.05) in gills, liver, and kidney tissues, whereas glutathione (GSH) and glutathione peroxidase (GPx) (except liver tissue) activities were inhibited when compared to the control group. Likewise, exposure to TCEP significantly (p < 0.05) altered the biochemical (glucose and protein) and electrolyte (sodium, potassium, and chloride) levels of fish. Light microscopic studies exhibited series of histopathological anomalies in the gills, liver, and kidney tissues. The present study reveals that TCEP at tested concentrations causes adverse effects on fish and the studied biomarkers could be used for monitoring the ecotoxicity of organophosphate esters (OPEs).

Ambient particulate matter (PM) newly has been regarded as a conceivable hazard for public health. A large number of studies have described that PM, exceptionally PM₂.₅, is correlated with respiratory, cardiovascular, and metabolic diseases, etc. PM₂.₅-induced hepatocyte steatosis previously has been uncovered both in cellular and murine models. Nevertheless, less is known about the underlying mechanism. Here, we found that PM₂.₅ could cause the downregulation of farnesoid X receptor (FXR), a key transcription factor for lipid metabolism. FXR could regulate the accumulation of lipid droplets induced by PM₂.₅ in vitro. Moreover, FXR⁻/⁻ mice were exposed to PM₂.₅ for 2 months to investigate the role of FXR in pathogenesis of PM₂.₅-induced hepatic steatosis in vivo. The results showed that exposure of wild-type (WT) mice to PM₂.₅ caused mild liver steatosis compared with the mice exposure to filtered air (FA). Furthermore, the content of triglyceride (TG) and total cholesterol (TC) was elevated in WT mice liver triggered by the inhalation of PM₂.₅. However, there was no statistical difference in TG and TC content between FXR⁻/⁻ mice with and without PM₂.₅ exposure. Overall, our finding suggested FXR mediated PM₂.₅-induced hepatic steatosis.

Many areas throughout the world, mainly arid and semi-arid regions, are simultaneously affected by salinity stress and heavy metal (HM) pollution. Phytoremediation of such environments needs suitable plants surviving under those combined stresses. In the present study, native species naturally growing under an extreme condition, around Qaleh-Zari copper mine located in the eastern part of Iran, with HM-contaminated saline–sodic soil, were identified to find suitable plant species for phytoremediation. For this purpose, the accumulation of HMs (Cu, Zn, Cd, and Pb) in the root and shoot (stem and leaf) of the plants and their surrounding soils was determined to find their main phytoremediation strategies: phytoextraction or phytostabilization. Seven native species surviving in such extreme condition were found, including Launaea arborescens (Batt.) Murb, Artemisia santolina Schrenk, Pulicaria gnaphalodes (Vent.) Boiss, Zygophyllum eurypterum Boiss. & Buhse, Peganum harmala L., Pteropyrum olivieri Jaub. & Spach, and Aerva javanica (Burm. f.) Juss. ex Schult. Evaluation of phytoremediation potential of the identified species based on the calculated HM bioconcentration in roots, HM translocation from roots to shoots, and HM accumulation in the shoots revealed that all of the species were metal phytostabilizers rather than hyperaccumulators. Therefore, these native species can be used for phytostabilization in the HM-contaminated saline soils to prevent HMs entering the uncontaminated areas and groundwater. Compared with the biennial low-biomass hyperaccumulators, some native species such as Z. eurypterum and A. javanica may have more economic value for phytoremediation because of a significant accumulation of HMs in their relatively higher biomass.