Photocatalysis was recognised as a suitable process for the photoabatement of atmospheric pollutants. The photooxidation mechanism on TiO2 has been widely studied. However, recent studies demonstrated that the very often-assumed photooxidation intermediated by the hydroxyl radical cannot explain all the experimental observations. Indeed, this study contributes for a new understanding of NO photooxidation. First, the adsorption equilibrium isotherms of NO, NO2 and H2O on the photocatalyst, Aeroxide® P25 from Evonik Industries, were obtained. Also, the concentration of hydroxyl radicals was determined by photoluminescence. A comprehensive design of experiments was then followed; NO conversion and selectivity were obtained as a function of the relative humidity, irradiance, NO inlet concentration and residence time, following a response surface methodology (RSM). These results were then used to discuss the photooxidation mechanism of NO.

The abundance of silver nanoparticles (AgNPs) in consumer products has led to their environmental release and therefore to concern about their impact on human health. The ingestion of AgNP-contaminated soil from urban sites is an important exposure pathway, especially for children. Given the limited information on oral bioaccessibility of soil Ag, we used a physiologically based extraction test (PBET) to evaluate the bioaccessibility of AgNPs and AgNO₃ from soil digestion. The AgNPs underwent several biochemical transformations, including their simultaneous dissolution and agglomeration in gastric fluid followed by the disintegration in the intestinal fluid of the agglomerates into NPs containing silver and chlorine. Therefore, Ag-containing soil exposed the intestine to nanoparticulate Ag in forms that were structurally different from the original forms. The bioaccessibility of AgNPs (0.5 ± 0.05%–10.9 ± 0.7%) was significantly lower than that of AgNO₃ (4.7 ± 0.6%–14.4 ± 0.1%), as a result of the lower adsorption of nanoparticles to soil residues during the digestive process. For the soils tested, the bioaccessibility of AgNPs increased with decreasing clay contents and lower pH. By identifying the soil properties that control AgNP bioaccessibility, a more efficient and accurate screening can be performed of soil types that pose the greatest health risk associated with AgNP exposure.

Trace metal risk assessment and environmental quality standard definition require realistic models that quantify metal exposure and accumulation by biota. In the present study we propose a novel trait-based approach to predict whole body concentrations of metals in aquatic invertebrates from concentrations measured in different environmental compartments. Field data from a large riverine floodplain was used to calibrate and test the model. The prediction performance of the trait-based model was unbiased and uncertainty was below the twofold of measured concentrations for the four studied metals (Ni, Cu, Cd, Pb). The relative contribution of feeding, respiration and locomotion patterns as well as metal concentrations in three environmental compartments provided insights into the importance of different uptake pathways. The relation with the sediment (i.e., to what degree taxa live in or directly on the sediment) was shown to be the most important trait to predict metal accumulation. Overall, this study demonstrated the potential use of bioecological traits for the modeling of whole body metal concentrations of entire aquatic invertebrate communities.

Substantial evidence has linked short-term exposure to ambient fine particulate matter (PM2.5) with increased cardiovascular mortality, however, the specific chemical constituent and emission source responsible for this effect remained largely unclear. A time series Poisson model was employed to quantify the association of cardiovascular mortality with two sets of shipping pollution emission: nickel (Ni), vanadium (V) (the indices of shipping emission) and estimated shipping emission using a source apportionment approach in Guangzhou, China in 2014. We observed that Ni, V, and estimated shipping emission in PM2.5 were associated with increased cardiovascular mortality, an inter-quartile range (IQR) increase in lag2 Ni was associated with 4.60% (95% CI: 0.14%, 9.26%) increase in overall cardiovascular mortality, and 13.35% (95% CI: 5.54%, 21.75%) increase in cerebrovascular mortality; each IQR increase of lag1 V was correlated with 6.01% (95% CI: 1.83%, 10.37%) increase in overall cardiovascular mortality, and 11.02% (95% CI: 3.15%, 19.49%) increase in cerebrovascular mortality; and each IQR increase in lag1 shipping emission was associated with 5.55% (95% CI: 0.78%, 10.54%) increase in overall cardiovascular mortality, and 10.39% (95% CI: 1.43%, 20.14%) increase in cerebrovascular mortality. The results remained robust to adjustment for PM2.5 mass and gaseous air pollutants. This study suggests that shipping emission is an important detrimental factor of cardiovascular mortality, and should be emphasized in air pollution control and management in order to protect the public health in Guangzhou, China.

IMO’s Anti-Fouling Systems convention banned the use of organotin-based antifouling systems in 2008 as the ultimate effort to stop tributyltin (TBT) inputs into the marine environment. One of the hazardous effects of TBT is imposex (the superimposition of male sexual characters onto gastropod females), a phenomenon that may cause female sterility and the gastropod populations decline. Despite previous European Union legislation had already been shown effective in reducing the imposex levels along the Portuguese coast, this study intends to confirm these decreasing trends after 2008 and describe the global evolution in the last 15 years. Imposex levels were assessed in two bioindicators – the dog-whelk Nucella lapillus and the netted-whelk Nassarius reticulatus (Gastropoda, Prosobranchia) – in 2011 and 2014, and the results were compared with previous years. Both species showed progressive decreasing trends in imposex levels over the last 15 years; median values of the vas deferens sequence index (VDSI) fell from 3.96 to 0.78 in N. lapillus and from 3.39 to 0.29 in N. reticulatus. The temporal/spatial evolution of imposex suggests an apparent shift of TBT hotspots, being now restricted to fishing ports and marinas in detriment of large commercial harbours where TBT levels fell rapidly. Butyltins were measured in the whole tissues of N. lapillus females collected in 2014: monobutyltin (MBT) varied from < DL (detection limit: 1 ng Sn/g) to 13 ng Sn/g dw, dibutyltin (DBT) from 2.2 to 27 ng Sn/g dw and TBT from 1.5 to 55 ng Sn/g dw. Although TBT body burden has declined over time, the butyltin degradation index ([MBT]+[DBT])/[TBT] exhibited values < 1 in c. a. 90% of the sites assessed, suggesting that recent TBT inputs are still widespread in the Portuguese coast eventually due to illegal use of TBT antifouling systems and TBT desorption from sediments.

Fungi are well associated with the degradation of hydrocarbons by the production of different enzymes, among which catalases (CBH), laccases (LCC) and peroxidases (LiP and MnP) are of immense importance. In this study, crude oil tolerance and enzyme secretions were demonstrated by rhizospheric fungal strains. Four most abundant strains were isolated from the rhizosphere of grasses growing in aged oil spill sites and identified through morphological characterization and molecular PCR-amplification of 5.8–28S ribosomal rRNA using ITS1 and ITS4 primers. These strains were subjected to crude oil tolerance test at 0–20% concentrations. Presence and transcriptase responses of putative genes lig (1–6), mnp, cbh (1.1, 1.1 and 11), and lcc encoding lignin peroxidase, manganese peroxidase, catalase, and laccase enzymes respectively were also studied in these strains using RT-PCR. In addition, activities of secreted enzymes by each strain were studied in aliquots. The strains were identified as Aspergillus niger asemoA (KY473958), Talaromyces purpurogenus asemoF (KY488463), Trichoderma harzianum asemoJ (KY488466), and Aspergillus flavus asemoM (KY488467) through sequencing and comparing the sequences’ data at NCBI BLAST search software. All the isolated strains showed tolerance to crude oil at 20% concentration, but the growth rate reduced with increasing in oil concentrations. All the isolated strains possess the tested genes and lig 1–6 gene was overexpressed in A. niger and T. harzianum while lcc and mnp genes were moderately expressed in all the four strains. Almost 145 U.mL⁻¹ of lignin and manganese peroxidase, 87 U.mL⁻¹ of catalase, and 180 U.mL⁻¹ of laccase enzymes were produced by these strains and it was also observed that these strain mostly produced studied enzymes in response to increasing crude oil concentrations. Considering the robust nature and diverse production of these catalytic enzymes by these strains, they can be exploited for various bioremediation technologies as well as other biotechnological applications.

Air pollution has been shown to be significantly associated with morbidity and mortality in urban areas, but there is lack of studies focused on extreme pollution events such as extreme dust episodes in high-density Asian cities. However, such cities have had extreme climate episodes that could have adverse health implications for downwind areas. More importantly, few studies have comprehensively investigated the mortality risks of extreme dust events for socioeconomically vulnerable populations.This paper examined the association between air pollutants and mortality risk in Hong Kong from 2006 to 2010, with a case-crossover analysis, to determine the elevated risk after an extreme dust event in a high-density city. The results indicate that PM₁₀₋₂.₅ dominated the all-cause mortality effect at the lag 0 day (OR: 1.074 [1.051, 1.098]). This study also found that people who were aged ≥ 65, economically inactive, or non-married had higher risks of all-cause mortality and cardiorespiratory mortality during days with extreme dust events. In addition, people who were in areas with higher air pollution had significantly higher risks of all-cause mortality and cardiorespiratory mortality.In conclusion, the results of this study can be used to target the vulnerable among a population or an area and the day(s) at risk to assist in health protocol development and emergency planning, as well as to develop early warnings for the general public in order to mitigate potential mortality risk for vulnerable population groups caused by extreme dust events.

Increased generation of shrimp shell from exploitation of krill results in emerging biowaste pollution, in addition, uranium pollution has drawn public concern due to the rapid development of nuclear power, uranium mining, and nuclear fuel processing. In this study, krill shells were recovered and used as a potential natural biosorbent for uranium immobilization, thereby enabling both uranium decontamination and krill shell reutilization. Interaction of uranium with krill shell surface and their transformation were investigated by using batch sorption experiments, scanning electron microscopy, and transmission electron microscopy. Krill shell had high uranium sorption ability. Uranium was transformed into a nano-scale precipitate. The mapping of phosphorus and uranium was related to the nano-scale precipitate, indicating that sorption of uranium was dependent on phosphorus. Surface chemisorption between phosphate in krill shell and uranium as well as the formation of the nano-scale precipitate were interpreted as the mechanism of uranium immobilization. Thus, natural krill shell waste has potential for extensive use as a promising and cost-effective sorbent for uranium immobilization and krill shell reutilization.

During the last several decades, persistent organic pollutants and metals cause great concern for their toxicity in organisms as well as for their bioaccumulation and/or trophic transfer through the food chains in ecosystems. A large number of studies therefore have focused on the trophic levels of organisms to illustrate food web structure, as a critical component in the study of pollutant dynamics and biomagnification. The trends in biomagnification of pollutants in food webs indeed provide fundamental information about the properties and fates of pollutants in ecosystems. The trophic magnification supports the establishment of a reliable trophic structure, which can further aid the understanding of the transport and exposure routes of contaminants in accumulation and risk assessments. Recently, efforts to interpret the food web structure using carbon and nitrogen stable isotope ratios have contributed to better understanding of the fate of pollutants in the ecosystem. However, it is known that this isotope analysis of bulk ones has many weaknesses, particularly for uncertainties on the estimate of trophic levels and therefore of magnification factors for studied organisms, enough to support a regulatory interpretation. In this review, we collate studies that investigated biomagnification characteristics of pollutants in aquatic ecosystems, along with calculated trophic magnification factors. Moreover, we introduce a novel approach, compound-specific stable isotope analysis of nitrogen in amino acids, to establish reliable food web structures and accurate trophic levels for biomagnification studies. This method promises to provide sound results for interpreting the influence of the pollutant in organisms, along with their bioaccumulation and magnification characteristics, as well as that in ecosystem.

Soil algal bioassays have been limited by their inability to evaluate several toxic endpoints because it is difficult to collect pure soil algae growing on and beneath the soil surface. This study describes the extension of a previously developed paper-disc method for analyzing soil toxicity to algae. The method can be used in conjunction with flow cytometric analysis and facilitates the assessment of previously proposed toxicity endpoints, such as the growth zone, biomass, and photosynthetic activity. We assessed the applicability of this paper-disc soil method using the green algae Chlamydomonas reinhardtii and Pseudokirchneriella subcapitata exposed to nickel-contaminated soil; examined cell sizes, cell granularity, enzyme activity, and oxidative stress as new toxicity endpoints using flow cytometry; and identified morphological changes in green algae assayed. The results showed that, used in conjunction with flow cytometry, the extended paper-disc soil method is sufficiently sensitive to detect decreases in cell granularity in C. reinhardtii and esterase activity in P. subcapitata. The method also revealed decreases in growth zone, biomass, and electron transfer from the reaction center to the quinone pool. Collectively, the results of this study indicate that soil algal bioassays using nonspecific algae can be used to assess soil quality, to derive several toxicity endpoints for individual cells, and to evaluate previously established flow cytometric toxicity endpoints.