As typical pollutants, petroleum hydrocarbons that are widely present in various environmental media such as soil, water, sediments, and air, seriously endanger living organisms and human health. In the meantime, as a green environmental technology that integrates pollutant removal and resource recovery, bioelectrochemical systems (BESs) have been extensively applied to the removal of petroleum hydrocarbons from the environment. This review introduces working principles of BESs, following which it discusses the different reactor structures, application progresses, and key optimization factors when treating water, sewage sludges, sediments, and soil. Furthermore, bibliometrics was first used in this field to analyze the evolution of knowledge structure and forecast future hot topics. The research focus has shifted from the early generation of bioelectric energy to exploring mechanisms of soil remediation and microbial metabolisms, which will be closely integrated in the future. Finally, the future prospects of this field are proposed. This review focuses on the research status of bioelectrochemical degradation of petroleum hydrocarbons and provides a scientific reference for subsequent research.

In this study, we treated harmful Microcystis aeruginosa cyanobacteria using chitosan-modified nanobubbles. The chitosan-modified nanobubbles (255 ± 19 nm) presented a positive zeta potential (15.36 ± 1.17 mV) and generated significantly (p < 0.05) more hydroxyl radicals than the negatively charged nanobubbles (−20.68 ± 1.11 mV). Therefore, the interaction between the positively charged chitosan-modified nanobubbles and negatively charged M. aeruginosa (−34.81 ± 1.79 mV) was favored. The chitosan-modified nanobubble treatment (2.20 × 10⁸ particles mL⁻¹) inactivated 73.16% ± 2.23% of M. aeruginosa (2.00 × 10⁶ cells mL⁻¹) for 24 h without causing significant cell lysis (≤0.25%) and completely inhibited the acute toxicity of M. aeruginosa toward Daphnia magna. The inactivation was correlated (r² = 0.97) with the formation of reactive oxygen species (ROS) in M. aeruginosa. These findings indicated that the hydroxyl radicals generated by the chitosan-modified nanobubbles disrupted cell membrane integrity and enhanced oxidative stress (ROS formation), thereby inactivating M. aeruginosa. Moreover, the penetration of the chitosan-modified nanobubbles and cell alterations in M. aeruginosa were visually confirmed. Our results suggested that the chitosan-modified nanobubble treatment is an eco-friendly method for controlling harmful algae. However, further studies are required for expanding its practical applications.

Ustiloxin A (UA) and ustiloxin B (UB), two major mycotoxins produced by the pathogen of rice false smut (RFS) during rice cultivation, have attracted increasing attentions due to their potential health risks. However, limited data are available about their occurrence and fate in paddy fields and contamination profiles in rice. In this study, a field study was performed to investigate the occurrence and translocation of UA and UB in RFS-occurred paddies. For the first time to our knowledge, we reported a ubiquitous occurrence of the two ustiloxins in the paddy water (range: 0.01–3.46 μg/L for UA and <0.02–1.15 μg/L for UB) and brown rice (range: 0.09–154.08 μg/kg for UA and <0.09–23.57 μg/kg for UB). A significant positive correlation was observed between ustiloxin levels in paddy water and brown rice (rₛ = 0.48–0.79, p < 0.01). The occurrence of ustiloxin uptake in water-rice system was also evidenced by the rice exposure experiment, suggesting paddy water might be an important source for ustiloxin accumulation in rice. These results suggested that the contamination of ustiloxins in rice might occur widely, which was supported by the significantly high detection frequencies of UA (96.6%) and UB (62.4%) in polished rice (149 samples) from Hubei Province, China. The total concentrations of ustiloxins in the polished rice samples collected from Hubei Province ranged from <20.7 ng/kg (LOD) to 55.1 μg/kg (dry weight). Further studies are needed to evaluate the potential risks of ustiloxin exposure in the environment and humans.

Arbuscular mycorrhizal fungi display desired potential to boost crop productivity and drought acclimation. Yet, whether nanoparticles can be incorporated into arbuscular mycorrhizal fungi for better improvement and its relevant morphologic and anatomical evidences are little documented. Pot culture experiment on wheat (Triticum aestivum L.) was conducted under drought stress (30% FWC) as well as well watered conditions (80% FWC) that involved priming of wheat seeds with iron nanoparticles at different concentrations (5mg L⁻¹, 10 mg L⁻¹ and 15 mg L⁻¹) with and without the inoculation of Glomus intraradices. The effects of treatments were observed on morphological and physiological parameters across jointing, anthesis and maturity stage. Root colonization and nanoparticle uptake trend by seeds and roots was also recorded. We observed strikingly high enhancement in biomass up to 109% under drought and 71% under well-watered conditions, and grain yield increased to 163% under drought and 60% under well-watered conditions. Iron nanoparticles at 10 mg L⁻¹ when combined with Glomus intraradices resulted in maximum wheat growth and yield, which mechanically resulted from higher rhizosphere colonization level, water use efficiency and photosynthetic rate under drought stress (P < 0.01). Across growth stages, optical micrograph observations affirmed higher root infection rate when combined with nanoparticles. Transmission electron microscopy indicated the penetration of nanoparticles into the seeds and translocation across roots whereas energy dispersive X-ray analyses further confirmed the presence of Fe in these organs. Iron nanoparticles significantly enhanced the growth-promoting and drought-tolerant effects of Glomus intraradices on wheat.

Groundwater constitutes a major source of fresh water globally. However, it faces serious quality challenges from both conventional pollutants and contaminants of emerging concern (CECs) such as pharmaceutically active compounds (PhACs), personal care products (PCPs) and pesticides. There exists a significant knowledge gap regarding the occurrence of CECs in groundwater, especially in Africa. This study presents unique data on the concentration of fourteen PhACs, five PCPs and nine pesticides in groundwater wells in Nzoia River basin, Kenya. Generally, PCPs were the most dominant class with concentrations up to 10 μg/L (methylparaben). Anti(retro)virals, being important in the treatment of HIV/AIDS, were more prevalent among the PhACs as compared to the developed world, with concentrations up to 700 ng/L (nevirapine). In contrast, pesticides were measured at lower concentrations, the maximum being 42 ng/L (metolachlor). A basic risk assessment shows that – among the detected CECs – carbamazepine may pose medium human health risk and requires further investigation among infants and children. Point-of-use (POU) technologies are being increasingly promoted especially in the developing nations to provide drinking water solutions at the household level, but very little data is available on their performance towards CECs removal. Therefore, besides measuring CECs in groundwater, we investigated ceramic filters and solar disinfection (SODIS) as possible POU treatment options. Both techniques show potential to treat CECs in groundwater, with removal efficiencies higher than 90% obtained for 41 and 22 compounds in ceramic filters and SODIS, respectively. Moreover, for the more recalcitrant compounds (e.g. sulfadoxin), the performance is improved by up to three orders of magnitude when using TiO₂ as a photocatalyst in SODIS.

GABA, a four-carbon non-protein amino acid, plays an important role in animals and plants. We previously found GABA could alleviate alkali stress in apple seedlings. However, its physiological mechanism under heavy metal cadmium (Cd) stress need to be further studied. Thus, we explored its biological role in response to Cd stress. It was verified that 0.5 mM GABA could effectively alleviate Cd toxicity. Using NMT technique, we found that exogenous GABA could significantly reduce the net Cd²⁺ fluxes in apple roots, and Cd content was significantly lower than that in roots under Cd stress. Further analysis indicated exogenous GABA could significantly reduce the expression of genes related to the uptake and transport of Cd in apples under Cd stress. In addition, exogenous GABA could significantly increase the content of amino acids in apple roots under Cd stress. GAD is a key enzyme in GABA synthesis, we obtained transgenic apple roots of overexpression MdGAD1. Compared with the control, transgenic roots accumulated less Cd, maintained lower Cd uptake by roots, and lower expression of related transport genes. These results showed that GABA could effectively alleviate Cd toxicity in apple seedlings and provide a new perspective of GABA to alleviate Cd stress.

Globally, amphibian species are experiencing dramatic population declines, and many face the risk of imminent extinction. Endocrine-disrupting chemicals (EDCs) have been recognised as an underappreciated factor contributing to global amphibian declines. In this regard, the use of hormonal growth promotants in the livestock industry provides a direct pathway for EDCs to enter the environment—including the potent anabolic steroid 17β-trenbolone. Emerging evidence suggests that 17β-trenbolone can impact traits related to metabolism, somatic growth, and behaviour in non-target species. However, far less is known about possible effects of 17β-trenbolone on anuran species, particularly during early life stages. Accordingly, in the present study we investigated the effects of 28-day exposure to 17β-trenbolone (mean measured concentrations: 10 and 66 ng/L) on body size, body condition, metabolic rate, and anxiety-related behaviour of tadpoles (Limnodynastes tasmaniensis). Specifically, we measured rates of O₂ consumption of individual tadpoles as a proxy for metabolic rate and quantified their swimming activity and their time spent in the upper half of the water column as indicators of anxiety-related behaviour. Counter to our predictions based on effects observed in other taxa, we detected no effect of 17β-trenbolone on body size, metabolic rate, or behaviour of tadpoles; although, we did detect a subtle, but statistically significant decrease in body condition at the highest 17β-trenbolone concentration. We hypothesise that 17β-trenbolone may induce taxa-specific effects on metabolic function, growth, and anxiety-related behaviour, with anurans being less sensitive to disruption than fish, and encourage further cross-taxa investigation to test this hypothesis.

Tire wear particles (TWPs), which are among the microplastic pollutants in the environment, can inevitably accumulate in coastal sediments. The present study comprehensively investigated the effect of pristine TWPs on bacterial community structure in coastal sediments and compared the effect of pristine TWPs and aged TWPs on nine strains of bacteria in sediments. In addition, the effect of the TWP leachate was studied with all the nine bacterial strains and the toxicity-causing substances in the leachate was investigated using Bacillus subtilis. Exposure to TWPs could lead to a shift in bacteria community and affect nitrogen metabolism in marine sediments. Aged TWPs were more toxic than pristine TWPs due to changes in particle surface characteristics. The leachate exhibited greater toxicity than TWPs as well, and Zn was identified to be the major toxicity-causing substance. The overall results of this study are important for understanding the effects of TWPs and the leachates on microorganisms in marine sediments.

Studies on the human body burden of dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) in populations around municipal solid waste incinerators (MSWIs) in China are limited. The objective of this study was to assess the potential adverse health effects of an 8-year MSWI on the surrounding population and identify possible exposure pathways. We hypothesized that the MSWI would result in different environmental impacts and population health outcomes between upwind and downwind of its 3 km vicinity. We conducted a 10-year retrospective mortality survey on the population surrounding the MSWI. Then, we selected 50 residents aged 50 years or older on each of the upwind and downwind sides of MSWI to test serum PCDD/Fs. Meanwhile, environmental and food exposures to PCDD/Fs were tested for selected residents. The age-adjusted mortality rates were significantly higher for residents downwind than upwind, but no significant difference was found in the standardized mortality ratio before and after the MSWI operation. The toxic equivalents (TEQ) and major congeners of PCDD/Fs were significantly higher in the sera of the downwind residents than in the upwind. The PCDD/Fs in air, soil, dust, and vegetables on the downwind side were not significantly different from those on the upwind side, but the mean concentrations of PCDD/Fs in downwind hen eggs was significantly higher than those from upwind. In conclusion, downwind residents living within 3 km of the MSWI had higher age-adjusted mortality and serum level of PCDD/Fs than upwind residents. This higher mortality rate among downwind residents was not associated with MSWI. However, the higher levels of PCDD/Fs in downwind hen eggs suggest that the downwind population dioxin exposure was related to their location.

Soil heavy metal contamination has increasingly become a serious environmental issue globally, nearing crisis proportions. There is an urgent need to find environmentally friendly materials to remediate heavy-metal contaminated soils. With the continuing maturation of research on using biochar (BC) for the remediation of contaminated soil, nano-biochar (nano-BC), which is an important fraction of BC, has gradually attracted increasing attention. Compared with BC, nano-BC has unique and useful properties for soil remediation, including a high specific surface area and hydrodynamic dispersivity. The efficacy of nano-BC for immobilization of non-degradable heavy-metal contaminants in soil systems, however, is strongly affected by plant rhizosphere processes, and there is very little known about the role that nano-BC play in these processes. The rhizosphere represents a dynamically complex soil environment, which, although having a small thickness, drives potentially large materials fluxes into and out of plants, notably agricultural foodstuffs, via large diffusive gradients. This article provides a critical review of over 140 peer-reviewed papers regarding nano-BC-rhizosphere interactions and the implications for the remediation of heavy-metal contaminated soils. We conclude that, when using nano-BC to remediate heavy metal-contaminated soil, the relationship between nano-BC and rhizosphere needs to be considered. Moreover, the challenges to extending our knowledge regarding the environmental risk of using nano-BC for remediation, as well as further research needs, are identified.