The effects of floating macroalgae (250gDWm⁻²) on photoautotrophic microbenthos were studied in a flow-through mesocosm with a parallel mesocosm without macroalgae serving as Control. Vertical microprofiles of O₂ at the sediment–water interface showed a immediate and complete suppression of photosynthetic activity of microphytobenthos (MPB) under the macroalgal canopy, resulting in a shift of benthic metabolism from autotrophic to heterotrophic. MPB abundance and chlorophyll a content decreased and a change from a diatom-dominated to cyanobacteria-dominated community was observed. Inorganic nitrogen nutrients´ concentrations increased in the porewater as a result of the inhibition of MPB nutrient demand, leading to an increase in net ammonification and anaerobic NOₓ ⁻ consumption rates. No organic matter transfer from macroalgae to the sediment was detected, resulting in a net consumption of the carbon and nitrogen stored in the sediment. In consequence, sediment was progressively impoverished in nitrogen, reducing sediment nutrient regeneration.

Linear Alkylbenzene Sulfonates (LAS) are ubiquitous surfactants. Traces can be found in coastal environments. Sorption and toxicity of C₁₂-LAS congeners were studied in controlled conditions (2–3500μg C₁₂LAS/L) in five marine phytoplanktonic species, using standardized methods. IC₅₀ values ranged from 0.5 to 2mg LAS/L. Sorption of ¹⁴C₁₂-6 LAS isomer was measured at environmentally relevant trace levels (4μg/L) using liquid scintillation counting. Steady-state sorption on algae was reached within 5h in the order dinoflagellate>diatoms>green algae. The sorption data, fitted a L-type Freundlich isotherm, indicating saturation. Desorption was rapid but a low LAS fraction was still sorbed after 24h. Toxic cell concentration was 0.38±0.09mg/g for the studied species. LAS toxicity results from sorption on biological membranes leading to non-specific disturbance of algal growth. Results indicate that LAS concentrations in coastal environments do not represent a risk for these organisms.

Polybrominated diphenyl ethers (PBDEs) are now found ubiquitously in the aquatic environment and biota, and there is a growing concern that PBDEs may disrupt endocrine systems, leading to reproductive impairments of aquatic animals. In our study, zebrafish (Danio rerio) were exposed to the 5ng/L, 1μg/L and 50μg/L of DE-71 for the duration of the whole life cycle (120days, from eggs to adults). The expression of selected genes along the brain–pituitary–gonadal (BPG) axis and liver, and the levels of plasma sex hormones were examined. In male fish, up-regulation of GnRH in brain, FSHβ and LHβ in pituitary, FSH-receptor, LH-receptor, and CYP19a in testis was clearly evident, while down-regulation of CYP11a and 3β-HSD was found in testis. In female fish, a 2.4-fold up-regulation of 3β-HSD was found in ovary upon exposure to 50μg/L of DE-71. GnRH in brain, FSHβ and LHβ in pituitary were also up-regulated, while ERβ, TH and TPH in brain and GnRH-receptor in pituitary were significantly down-regulated. Hepatic ERα, AR and VTG in males were all down-regulated, while hepatic ERα and AR in female were up-regulated. Serum estradiol (E₂) was reduced in both male and female upon exposure to DE-71, while significant increases in serum testosterone (T) and 11-keto-testosterone (11-KT) were only found in male but not female fish. The ratio of T/E₂ as well as the ratio of 11-KT/E₂ in male fish increased in a dose-dependent manner upon exposure to DE-71. Our overall results showed that whole life exposure of DE-71 altered the expression of regulatory genes and receptors at all three levels of the BPG axis in zebrafish, and the responses are sex dependent. The observed disruption of GnRH and GtHs can be further related to the subsequent disruption in both levels and balance sex steroid hormones.

Sediments and sponges were collected from various locations along the eastern coast of the Red Sea, the Kingdom of Saudi Arabia. Total concentrations of Cd, Zn, Ag, Cu, Pb, As and Hg in the sediments were measured. Metal contamination was not significant in most of the studied sites and only one site was moderately polluted by Zn, Cu, and Pb. Sponges accumulated specific metals readily even though the metal exposure was low in the ambient environment. Contrasting interspecies differences in metal accumulation patterns were observed among the nine collected species of sponges. Significant positive correlations were found between the metal concentrations in the two species of sponges collected from the same sites. The strong ability to accumulate specific metals and the diversity of sponges that live in the Red Sea coastal areas make them a promising biomonitor of metal contamination in the areas.

In Korea, the new permission criteria for industrial effluents based on Daphnia magna acute toxicity tests will be gradually implemented starting from 2011. Thus, in this study, toxicity assessment and identification using a marine species (Tigriopus japonicus) and the freshwater species (D. magna) was comparatively investigated. Effluent from an acid mine drainage treatment plant showed acute toxicity toward both organisms due to low pH, which was removed by neutralization of the effluent. Additionally, evaluation of the effluent of an electronics company revealed that Cu was attributable to the observed toxicity, and the effluent was more toxic toward T. japonicus than D. magna. Moreover, effluents from a metal plating factory were acutely toxic toward D. magna (6.50 TU), while they were not toxic against T. japonicus. Toxicity identification revealed that the high level of Cl⁻ (12,841mgL⁻¹) was the cause of toxicity. Thus, the effluents had no effect on the marine species, T. japonicus. These findings suggest that a marine species rather than a freshwater species is more desirable for toxicity assessment of industrial effluent discharged into the saltwater, and thus should be considered in the legislation of toxicity-based discharge limits in Korea.

To evaluate the superoxide dismutase (SOD) genes as a potential indicator of environmental stress, we cloned and sequenced the full-length cDNA of a Cu/Zn-SOD and Mn-SOD genes from the marine polychaete, Neanthes succinea. Ns-Cu/Zn- and Mn-SOD expression was measured using quantitative real-time RT-PCR in response to the oxidative stress induced by copper in the form of CuCl₂. Ns-Cu/Zn-SOD expression increased in a dose dependent manner but the increase of Mn-SOD expression was evident only at a high concentration of copper. Particularly, Ns-Mn-SOD dramatically increased in the 72μg/L CuCl₂-exposed groups, indicating that both Ns-SOD genes were induced by copper exposure. These results suggest that Ns-Cu/Zn- and Ns-Mn-SOD would play important roles in the detoxification of cellular oxidative stresses in the polychaete, N. succinea. Furthermore, as part of field work, the marine polychaete, N. succineaCu/Zn-SOD mRNA collected from the polluted area was accordingly upregulated in expression but not for NS-Mn-SOD.

The potential of 2,2′,4,4′-tetrabromodiphenyl ether (BDE47) and its related hydroxylated analogs (2′-HO-BDE28, 6-HO-BDE47, 4′-HO-BDE17, and 4′-HO-BDE49) to modulate estrogen/thyroid/androgen receptor-(ER, TR, AR), mediated responses were investigated by use of reporter gene assays. Exposure to 1 or 10μM, 4′-HO-BDE17 significantly up-regulated expression of Luc, whereas other four chemicals did not induce Luc expression under control of the ER. Anti-estrogenic potency was observed for 4′-HO-BDE17 (IC50=1.14μM)>6-HO-BDE47 (IC50=2.65μM)>2′-HO-BDE28 (IC50=9.49μM)>BDE47 (IC50=21.11μM). No anti-estrogenic effect of 4′-HO-BDE49 was observed. Both 4′-HO-BDE17, 4′-HO-BDE49 resulted in greater responses of Luc expression induced by T3. BDE47, 2′-HO-BDE28, 6-HO-BDE47 did not show any effect on the expression of Luc induced by 5nM T3. 6-HO-BDE47 (IC50=0.34μM)>4′-HO-BDE17 (IC50=1.41μM)>BDE47 (IC50=3.83μM)>2′-HO-BDE28 (IC50=29.22μM) exhibited anti-androgenic potency, while 4′-HO-BDE49 did not show androgenic transcriptional activity.

The Seine’s estuary (France) waters are the receptacle of effluents originating from wastewater treatment plants (WWTP). In this estuary, mudflats are deposition zones for sediments and their associated contaminants, and play an essential role in the mercury (Hg) biogeochemical cycle mainly due to indigenous microorganisms. Microcosms were used to assess the impact of WWTP-effluents on mercury methylation by monitoring Hg species (total dissolved Hg in porewater, methylmercury and total mercury) and on microbial communities in sediments. After effluent amendment, methylmercury (MeHg) concentrations increased in relation with the total Hg and organic matter content of the WWTP-effluents. A correlation was observed between MeHg and acid-volatile-sulfides concentrations. Quantification of sulfate-reducing microorganisms involved in Hg methylation showed no increase of their abundance but their activity was probably enhanced by the organic matter supplied with the effluents. WWTP-effluent spiking modified the bacterial community fingerprint, mainly influenced by Hg contamination and the organic matter amendment.

Since 1990s, various booster biocides have been increasingly used as substitutes of organotins. However, knowledge about their toxicities on tropical/sub-tropical marine species is significantly lacking. This study comprehensively investigated the acute toxicities of copper, tributyltin (TBT), and five commonly used booster biocides including Irgarol, diuron, zinc pyrithione (ZnPT), copper pyrithione (CuPT) and chlorothalonil on the growth or survival of 12 marine species in which eight of them are native species of subtropical Hong Kong. We found that Irgarol was more toxic than TBT on the growth of autotrophic species. The toxicity of CuPT was comparable to that of TBT on almost all test species, while it showed higher toxicity than TBT on medaka fish larvae. As the usage of these biocides is expected to further increase worldwide, accurate assessments of their ecological risks are required for better informed decision on their management. This study provided useful datasets for such purposes.

This study determined the concentrations of major and trace elements in shellfish (oysters, clams and mussels) and conducted an assessment of the health risks due to the consumption of contaminated seafood. Samples were collected at 34 sites along Todos os Santos Bay, Brazil. The elements were determined by ICP OES and Hg by Direct Mercury Analysis. Relatively high concentrations of trace elements (As, Zn, Se and Cu) were found in seafood tissues. Potential daily intake of As, Co, Se, Zn and Cu associated to shellfish consumption suggested relevant non-carcinogenic risk for all studied locations. Copper was the element that posed the greatest non-carcinogenic risk, while Pb posed the highest carcinogenic risk. Health risks for humans were greatest from the consumption of mussels. Contaminated shellfish offer the greatest risk for children, subsistence fishers and subsistence shellfish consumers.