Ethylene regulates plant root growth and resistance to environment stress. However, the role and mechanism of ethylene signaling in response to Cd stress in rice remains unclear. Here, we revealed that ethylene signaling plays a positive role in the resistance of rice to Cd toxicity. Blocking the ethylene signal facilitated root elongation under normal conditions, but resulted in severe oxidative damage and inhibition of root growth under Cd stress. Conversely, ethylene signal enhancement by EIN2 overexpression caused root bending, similar to the response of roots to Cd stress, and displayed higher Cd tolerance than the wildtype (WT) plants. Comparative transcriptome analysis indicated EIN2-mediated upregulation of genes involved in flavonoid biosynthesis and peroxidase activity under Cd stress. The synthesis of phenolic acids and flavonoids were positively regulated by ethylene. Thus, the ein2 (ethylene insensitive 2) mutants displayed lower ROS scavenging capacity than the WT. Moreover, a significant increase in Cd accumulation and relatively increased apoplastic flow were observed in the root apex of the ein2 mutant compared with the WT plants. Overall, EIN2-mediated Cd resistance in rice is mediated by the upregulation of flavonoid biosynthesis and peroxidase activity to induce ROS scavenging, and apoplastic transport barrier formation reduces Cd uptake.

Seven organophosphate esters (OPEs) in atmospheric particles and surface seawater were observed during a cruise in the western South China Sea (SCS) in 2014. The median concentrations of ∑OPEs were 688 pg/m³ and 5.55 ng/L for particle and seawater samples, respectively. Total OPEs were dominated by tris(1-chloro-2-propyl) phosphate (TCPP) and tris(2-chloroethyl) phosphate (TCEP). The spatial distribution of OPEs indicates that the OPEs in particle phase were mainly influenced by the air masses originating from China, Indochina Peninsula and Malay Archipelago, showing the significant contribution of anthropogenic sources from these regions. Significant positive correlations between Tri-n-butylphosphate (TnBP) and organic carbon (P < 0.05) in particle phase over the western SCS suggests that it might be a potential tracer for the source regions of Indochina Peninsula and Malay Archipelago. The spatial distribution of OPEs in seawater was contributed by freshwater inputs associating with variations of human activities as well as salinity. Seawater pollution levels of OPEs in the eastern coast of Vietnam were increased compared to those measured in the northern SCS. The loadings of ∑OPEs transported to the vast area of western SCS vias atmospheric deposition and air−seawater gas exchange were estimated to be 59 tons/year and 105 tons/year, respectively. This work highlights the importance of transport processes and air-seawater interface behavior of OPEs in the oceanic area.

Household air pollution (HAP) arising from combustion of biomass fuel (BMF) is a leading cause of morbidity and mortality in low-income countries. Air pollution may stimulate pro-inflammatory responses by activating diverse immune cells and cyto/chemokine expression, thereby contributing to diseases. We aimed to study cellular immune responses among women chronically exposed to HAP through use of BMF for domestic cooking. Among 200 healthy, non-smoking women in rural Bangladesh, we assessed exposure to HAP by measuring particulate matter 2.5 (PM₂.₅), black carbon (BC) and carbon monoxide (CO), through use of personal monitors RTI MicroPEM™ and Lascar CO logger respectively, for 48 h. Blood samples were collected following HAP exposure assessment and were analyzed for immunoprofiling by flow cytometry, plasma IgE by immunoassay analyzer and cyto/chemokine response from monocyte-derived-macrophages (MDM) and -dendritic cells (MDDC) by multiplex immunoassay. In multivariate linear regression model, a doubling of PM₂.₅ was associated with small increments in immature/early B cells (CD19⁺CD38⁺) and plasmablasts (CD19⁺CD38⁺CD27⁺). In contrast, a doubling of CO was associated with 1.20% reduction in CD19⁺ B lymphocytes (95% confidence interval (CI) = -2.36, −0.01). A doubling of PM₂.₅ and BC each was associated with 3.12% (95%CI = −5.85, −0.38) and 4.07% (95%CI = −7.96, −0.17) decrements in memory B cells (CD19⁺CD27⁺), respectively. Exposure to CO was associated with increased plasma IgE levels (beta(β) = 240.4, 95%CI = 3.06, 477.8). PM₂.₅ and CO exposure was associated with increased MDM production of CXCL10 (β = 12287, 95%CI = 1038, 23536) and CCL5 (β = 835.7, 95%CI = 95.5, 1576), respectively. Conversely, BC exposure was associated with reduction in MDDC-produced CCL5 (β = −3583, 95%CI = −6358, −807.8) and TNF-α (β = −15521, 95%CI = −28968, −2074). Our findings suggest that chronic HAP exposure through BMF use adversely affects proportions of B lymphocytes, particularly memory B cells, plasma IgE levels and functions of antigen presenting cells in rural women.

Beaches are an integral part of coastal tourism, but they are deteriorated by the beachgoers and recreational activities due to lack of adequate beach environmental awareness and management. Litter is widely distributed in marine and coastal environment and has been considered a severe concern. In China investigations to determine the beach litter abundance and pollution level are limited. The aim of this study is to estimate spatio-temporal distribution and composition of litter on 10 well-known Qingdao tourist beaches, involving pollution level by beach quality indexes. Beach litter was collected within an area of 25 × 25 m² in both summer (May, June and July) and winter (Nov, Dec and Jan) seasons, and was classified into eight categories. The abundance of beach litter was found higher in summer (0.13 ± 0.04 items/m²) than in winter (0.04 ± 0.01 items/m²). Overall, the percentage of plastics were higher in both summer (23.48%) and winter (24.04%) than that of other litter categories. Based on Clean Coast Index, 70% of beaches were very clean, 25% clean, and 5% moderately clean. Beach Grade Index showed that 15% beaches were very good, 5% good, 55% fair, and 25% poor. 85% beaches constituted some quantity of hazardous litter and 15% had no hazardous litter for Hazardous Items index. The findings suggest that the sources of beach litter along Qingdao beaches mainly come from the recreational and tourist activities. The substantial quantity of litter is also being transported by ocean (tides or current), which are finally deposited along beachfront. Despite regular cleaning operation along most of Qingdao beaches, suggested management practices involve mitigation measures, source reduction, change in littering behavior to improve further quality of beaches.

Exposure measurement error is an important source of bias in epidemiological studies. We assessed the validity of employing ambient (outdoor) measurements as proxies of personal exposures at individual levels focusing on fine particles (PM₂.₅) and black carbon (BC)/elemental carbon (EC) on a global scale. We conducted a systematic review and meta-analysis and searched databases (ISI Web of Science, Scopus, PubMed, Ovid MEDLINE®, Ovid Embase, and Ovid BIOSIS) to retrieve observational studies in English language published from 1 January 2006 until 5 May 2021. Correlation coefficients (r) between paired ambient (outdoor) concentration and personal exposure for PM₂.₅ or BC/EC were standardized as effect size. We used random-effects meta-analyses to pool the correlation coefficients and investigated the causes of heterogeneity and publication bias. Furthermore, we employed subgroup and meta-regression analyses to evaluate the modification of pooled estimates by potential mediators. This systematic review identified thirty-two observational studies involving 1744 subjects from ten countries, with 28 studies for PM₂.₅ and 11 studies for BC/EC. Personal PM₂.₅ exposure is more strongly correlated with ambient (outdoor) concentrations (0.63, 95% confidence interval [CI]: 0.57–0.68) than personal BC/EC exposure (0.49, 95% CI: 0.38–0.59), with significant differences in ṝ (0.14, 95% CI: 0.03–0.25; p < 0.05). The results demonstrated that the health status of participants was a significant modifier of pooled correlations. In addition, the personal to ambient (P/A) ratio for PM₂.₅ and average ambient BC/EC levels were potential effect moderators of the pooled ṝ. The funnel plots and Egger's regression test indicated inevident publication bias. The pooled estimates were robust through sensitivity analyses. The results support the growing consensus that the validity coefficient of proxy measures should be addressed when interpreting results from epidemiological studies to better understand how strong health outcomes are affected by different levels of PM₂.₅ and their components.

Environmental cadmium (Cd) or high-fat diet (HFD) exposure alone are risk factors of male infertility. However, the effect and mechanism of co-exposure to HFD and Cd on sperm quality remain unclear. This study was aimed to explore the combined effects of HFD and Cd on spermatogenesis as well as its m6A-dependent mechanism in vivo and in vitro. As a result, co-exposure of HFD and Cd resulted in a significant decrease in the number of mature testicular seminiferous tubules and epididymis sperm quantity in mice, compared with Cd or HFD exposure alone. Correspondingly, the mRNAs expression of Smc3(spermatocytes marker), Acrv1(round spermatids marker) and Lzumo3(elongated spermatids marker) were downregulated in HFD and Cd group. Furthermore, combined exposure downregulated the expression of meiosis-related proteins (STRA8 and SYCP3), increased the m6A level of Stra8, and upregulated the expression of m6A-related proteins (METTL3 and YTHDF2) in mouse spermatocytes. Mechanistically, the above-mentioned impacts caused by co-exposure were markedly restored by Mettl3 siR and Ythdf2 siR. In addition, RNA stability assay showed that Ythdf2 siR obviously reversed co-exposure-increased Stra8 mRNA degradation rate in actinomycin-D-treated mouse spermatocytes. Meanwhile, excess ROS was observed in combined-exposure group, and a free radical scavenger N-tert-Butyl-α-phenylnitrone (PBN) attenuated co-exposure-upregulated expression of METTL3 and YTHDF2 in mouse spermatocytes. These results suggested that combination of HFD and Cd impaired spermatogenesis by degrading Stra8 in an m6A-YTHDF2-dependent manner via ROS activation.

Microplastics have been proven to be hotspots of bacterial pathogens and antibiotic resistance genes (ARGs). The enrichment of ARGs in microplastisphere, the specific niche for diverse microbial communities attached to the surface of microplastic, has attracted worldwide attention. By collecting 477 pairs of ARG abundance data belonging to 26 ARG types, based on the standardized mean difference (SMD) under the random effect model, we have performed the first meta-analysis of the ARG enrichment on microplastics in aquatic environments in order to quantitatively elucidate the enrichment effect, with comparison of non-microplastic materials. It was found that ARGs enriched on the microplastics were more abundant than that on the inorganic substrates (SMD = 0.26) and natural water environments (SMD = 0.10), but lower abundant than that on the natural organic substrates (SMD = −0.52). Furthermore, microplastics in freshwater tended to have a higher degree of ARG enrichment than those in saline water and sewage. The biofilm formation stage, structure, and component of microplastisphere may play a significant role in the enrichment of ARGs.

The impact of land use type on the content of potentially toxic elements (PTEs) in the soils of the Qinghai-Tibet Plateau (QTP) and the associated ecological and human health risks has drawn great attention. Consequently, in this study, top- and subsurface soil samples were collected from areas with four different land uses (i.e., cropland, forest, grassland, and developed area) and the total contents of Cr, Cd, Cu, Pb and Zn were determined. Geostatistical analysis, self-organizing map (SOM), and positive matrix factorization (PMF), ecological risk assessment (ERA) and human health risk assessment (HRA) were applied and used to classify and identify the contamination sources and assess the potential risk. Partial least squares path modeling (PLS-PM) was applied to clarify the relationship of land use with PTE contents and risk. The PTE contents in all topsoil samples surpassed the respective background concentrations of China and corresponding subsurface concentrations. However, the ecological risk of all soil samples remained at a moderate or considerable level across the four land use types. Developed area and cropland showed a higher ecological risk than the other two land use types. Industrial discharges (32.8%), agricultural inputs (22.6%), natural sources (23.7%), and traffic emissions (20.9%) were the primary PTE sources in the tested soils, which indicate that anthropogenic activities have significantly affected soil PTE contents to a greater extent than other sources. Industrial discharge was the most prominent source of non-carcinogenic health risk, contributing 37.7% for adults and 35.2% for children of the total risk. The results of PLS-PM revealed that land use change associated with intensive human activities such as industrial activities and agricultural practices distinctly affected the PTE contents in soils of the Qinghai-Tibet Plateau.

In recent years, biochar has become of considerable interest for environmental applications, it can be used as a catalyst for sulfides reduction of perchloroethylene, but the crucial role of biochar properties played in catalyzing dechlorination remained ambiguous investigation. To pinpoint the critical functional groups, the modified biochars were respectively produced by HNO₃, KOH and H₂O₂ with similar dimensional structures but different functional groups. Combined with the adsorption and catalytic results of different biochars, the acid-modified biochar had the best catalytic performance (99.9% removal) due to the outstanding specific surface area and ample functional groups. According to characterization and DFT results, carboxyl and pyridine nitrogen exhibited a positive correlation with the catalytic rate, indicating that their contribution to catalytic performance. Customizing biochar with specific functional groups removed depth demonstrated that the carboxyl was essential component. Further, alkaline condition was conducive to catalytic reduction, while tetrachloroethylene cannot be reduced under acidic conditions, because HS⁻ and S²⁻ mainly existed in alkaline environment and the sulfur-containing nucleophilic structure formed with biochar was more stable under this condition. Overall, this study opens new perspectives for in situ remediation by biochar in chlorinated olefin polluted anoxic environment and promotes our insight of modifying for biochar catalyst design.

Numerous onshore oil production wells currently exist, and the petroleum hydrocarbon contamination of the surrounding soil caused by oil production wells is not well understood. Moreover, the impact of the distribution of the total petroleum hydrocarbons (TPH) in the soil on the microbiota requires further investigation. Accordingly, in this study, the distribution of petroleum hydrocarbons in the soils around oil production wells was investigated, and their alteration of the microbiota was revealed. The results revealed that in the horizontal direction, the heavily TPH-contaminated soils were mainly distributed within a circle with a radius of 200 cm centered on the oil production well; and in the vertical direction, the heavily TPH-contaminated soils were distributed within the 0–50 cm soil layer. A significant positive correlation was found between the microbial abundance and the TPH concentration in the soil with relatively low total carbon contents. Heavy TPH contamination (TPH concentration of >3000 mg/kg) significantly reduced the microbial diversity and altered the microbiota compared with the light TPH contamination (TPH concentration of around 1000 mg/kg). In the heavily TPH-contaminated soils, the relative abundances of the Proteobacteria and Bacteroides increased significantly; the network complexity among the soil microorganisms decreased; and the co-occurrence patterns were altered. In summary, the results of this study have reference value in the remediation of soils around oil production wells and provide guidance for the construction of microbial remediation systems for petroleum contamination.