Soil organic matter (SOM) affects arsenic (As) and antimony (Sb) mobility in soils under waterlogged conditions by acting as an electron donor, by catalyzing redox–cycling through electron shuttling and by acting as a competing ligand. This study was set up to disentangle these different effects of SOM towards As and Sb sorption in anaerobic soils. Nine samples were taken at different depths in an agricultural soil profile to collect samples with a natural SOM gradient (<1–40 g soil organic carbon kg⁻¹). The samples were incubated either or not under waterlogged conditions in an anaerobic chamber for 63–70 days, and glucose (5 g C kg⁻¹) was either or not added to the anaerobic incubated samples as an electron donor that neither acts as an electron shuttle nor as a competing ligand. The solid-liquid distribution coefficients (KD) of As and Sb were measured at trace levels. The KD values of As decreased ∼2 orders of magnitude upon waterlogging the SOM rich topsoil, while no additional changes were observed when glucose was added. In contrast, smaller changes in the As KD values were found in the low SOM containing subsoil samples, unless glucose was added that mobilised As. The Sb KD values increased upon reducing conditions up to factor 20, but again only in the high SOM topsoil samples. Surprisingly, the Sb immobilisation during waterlogging only occurred in Sb amended soils whereas the geogenic Sb was mobilised upon reducing conditions, although total dissolved Sb concentrations remained low (<10 nM). The change in As and Sb sorption upon waterlogging was similar in the SOM rich topsoil as in the low SOM subsoil amended with glucose. This suggests that the SOM dependent changes in As and Sb mobility in response to soil waterlogging are primarily determined by the role of SOM as electron donor.

Butylated hydroxytoluene (BHT) is a synthetic phenolic antioxidant that has been used as an additive for fat- or oil-containing foods. The exposure index value increases with extended usage of the chemical. Further, estimated total amount of BHT could exceed standard regulation, considering dietary intake or another exposure. Although BHT may induce side effects in reproductive systems, adequate research had not yet been performed to confirm them. In this study, we investigated the effects of BHT on mouse Leydig cells (TM3), which are components of testis. Our results indicated that BHT suppressed cellular proliferation and induced cell cycle arrest in TM3 cells. Moreover, BHT hampered cytosolic and mitochondrial calcium homeostasis in TM3 cells. Furthermore, BHT treatment led to endoplasmic reticulum (ER) stress and DNA fragmentation, simultaneously stimulating intrinsic apoptosis signal transduction. To elucidate the mode of action of BHT on Leydig cells, we performed western blot analysis and confirmed the activation of the PI3K/AKT and MAPK pathways. Collectively, our results demonstrated that BHT has toxic effects on mouse Leydig cells via induction of calcium dysregulation and ER-mitochondria dysfunction.

Soils in urban and industrial areas, especially in larger metropolitan areas such as the Ruhr area, Germany, are commonly characterized by severe anthropogenic overprinting due to urbanization processes including land development measures. Such urban soils often contain various anthropogenic substrate admixtures, like ash, coal, tailings, building rubble, industrial waste materials, as well as urban dust, soot, fly ash, and others. These admixtures often carry higher contents of pollutants such as polycyclic aromatic hydrocarbons (PAH). Whereas elevated PAH concentrations are commonly attributed to non-point pyrogenic carbon sources like soot and particulate matter, petrogenic PAH sources are still largely neglected in this context.In this study, an extended sample set of 62 samples of PAH source materials and urban soils containing anthropogenic substrate components was investigated by combining extended PAH analysis of 59 PAH, alkylated PAH distributions and benzene polycarboxylic acid (BPCA) analysis with regard to petrogenic and pyrogenic PAH source identification. For more reliability of source apportionment by a more integrative signal, the alkylated PAH distributions of different PAH groups were combined according to their degrees of alkylation. Based on this combination, a new PAH alkylation index (ΣC0/(ΣC0+ΣC2)) was derived, which considers, in contrast to commonly used single PAH ratios, a series of non-alkylated and alkylated PAH.By comparison of this PAH alkylation index with the degree of aromatic condensation a new robust and economic method for identifying petrogenic, pyrogenic and mixed PAH sources within soil samples and sediments was developed. It is shown that coal and coal ash particles are a not negligible PAH source in urban soils of mining-dominated regions and can make up a large proportion of the anthropogenic substrate components encountered.Further analyses of samples with defined levels of petrogenic and pyrogenic PAH are necessary to finally evaluate the usefulness of this proposed new PAH-BPCA approach.

Parameters such as the settling rate, aggregation rate, and collision frequency in predictive models used to describe the fate of nanoparticles (NPs) are very important for the risk assessment of NPs in the environment. In this study, CeO₂ NPs were chosen as the model particles to investigate such parameters through aggregation-settling experiments under environmentally relevant conditions. The results indicate that natural colloids (Ncs) have no effect on the settling of NPs in seawaters, whereas they stabilize the NPs at a low initial particle concentration and promote the heteroaggregation of NPs at a high initial particle concentration in lake waters. In all cases, a suspended sediment absorbs the NPs and Ncs as mixed aggregates, resulting in a rapid settling. Furthermore, the calculation results of the model indicate that the shear force increases the collision frequency of the NPs by 4–5 orders of magnitude higher than that in quiescent waters. However, the break-up effect by the shear force is more obvious, namely, the shear force hinders the aggregation of NPs in natural waters, instead of promoting aggregation. Remarkably, a negative value of the dis-heteroaggregation rate based on the combined von Smoluchowski–Stokes equation can reflect the hindering effect on the aggregation process. The results of this study will provide scientific and accurate guidance for the parameter selection in the existing prediction model and contribute to a prediction of the fate and transport of NPs in the environment.

Rapid economic growth, urban sprawl, and unplanned industrialization has increased socioeconomic statuses but also decreased air quality in South Asian developing countries. Therefore, severe increase in air pollution has been a threat of local population, regarding health statuses, livability and quality of life. It is necessary to estimate fine-scale spatiotemporal distribution of ambient PM₂.₅ in a national context so that the environmental planners and government officials can use it for environmental protocol development and policy-making. In this study, a spatiotemporal land use regression (LUR) model is developed to refine global air quality data to the national-scale ambient PM₂.₅ exposure in a high-density country in South Asia – Pakistan. Combining with transport network, patterns of land use, local meteorological conditions, geographic characteristics, landscape characteristics, and satellite-derived data, our resultant model explains 54.5% of the variation in ambient PM₂.₅ concentration level. Furthermore, tree coverage and road transport are identified to be two influential factors of the national-scale spatial variation of PM₂.₅ in Pakistan, which implied that urbanization might be the major cause of air pollution across the country. In conclusion, our resultant LUR model as well as the spatial map of ambient PM₂.₅ concentration level can be used as a supporting tool for national health risk management and environmental planning, and could also contribute to the air quality management and pollution reduction actions of Pakistan.

The textile mill industry is one of the major sources of pollution and contributors of metal contaminants to the environment. At the same time, the industry is important for global economy. Pollution caused by the textile industry is characteristic due to a unique set of potentially toxic substances. Darkling beetles (Coleoptera, Tenebrionidae), which live in all biogeographical regions, are especially common in soil quality and soil degradation studies. Our study was designed to assess long-term effects of textile industry (which generates specific pollution) on soil organisms, namely Trachyderma hispida. We especially wanted to find out what changes allow the species to survive and adapt to these specific conditions. Energy-dispersive X-ray spectroscopy of soil and midgut tissues of T. hispida sampled from a polluted site in the Edku textile industrial area in Egypt revealed a high accumulation of chemical elements, compared to a reference site. The concentration of elements in soil was well correlated with their concentration in the midgut of insects. Activity of superoxide dismutase, catalase, ascorbate peroxidase and glutathione S-transferase were negatively correlated with concentration of elements in soil and in the midgut. Meanwhile, malondialdehyde concentration in the midgut revealed an opposite tendency. DNA damage and expression of stress proteins, (HSP70 and metallothionein - MT) were elevated in insects from the polluted site. The activity of textile industry in the area of Edku undoubtedly causes an increase of soil pollution and, in consequence, causes a number of changes in the bodies of organisms living in these areas, including T. hispidus. Therefore, it is necessary to find a solution which limits the emission of waste from the textile industry, as well as to design modern strategies of processing, storing and utilizing it.

This study proposes an integrated cattle breeding and cultivation system that provides zero emission and sustainable livelihood for the community in rural areas. The proposed integrated farming system improves agricultural productivity and environmental and sanitation conditions, minimizes the amount of waste, and increases the family income up to 41.55%. Several waste types can be recycled and transformed into valuable products, such as energy for cooking, organic fertilizer for crops, and cattle feed for breeding. Wastewater effluent from the biogas tank can be treated by biochar and results show that it then meets the standards for irrigation purposes. Also, the waste flow from cattle breeding supplies enough nutrients to cultivate plants, and the plants grown supply are adequate food for the 30 cows living on the farm. This research shows that the use of an integrated farming system could achieve zero-emission goal. Thereby, it provides a sustainable livelihood for cattle breeding family farms. The proposed integrated cattle breeding and cultivation system improves agricultural productivity, environmental and increases the farmer income up to 41.55%.

Microplastics pose threats to aquatic environments because they serve as hard-substrate for microbial community colonization and biofilm formation due to their long-life span and hydrophobic surface which can impact on aquatic ecosystems. However, the association between microplastics and other pollutants, particularly nutrients and metals in river sediments are largely unknown. In this study, microplastics abundance and hazard scores which are the risks arising from chemical compounds used for plastics manufacture, and the correlations between microplastics and the concentrations of total carbon (TC), total nitrogen (TN), total phosphorus (TP) and metals commonly present in the urban environment such as Al, As, Cr, Co, Cu, Fe, Mg, Mn, Ni, Cd, Se, Sr, Zn, Pb, in Brisbane River sediments were investigated. The study confirmed that the risk associated with microplastics is based on their monomer composition rather than the quantities present. Sediments having relatively higher abundance of microplastics with a relatively lower hazard score result in higher nutrient concentrations. The concentrations of metals in river sediments are more dependent on their original sources rather than the concentration of microplastics. Nevertheless, leachate from plastics should be considered in risk assessment in relation to the association between metals and plastics in aquatic environments.

Psychoactive substances are becoming a new concern in aquatic environment along with the increase in use of these substances. In this study, 23 psychoactive substances were investigated in the tap water collected in 63 sites in China. Eighteen out of 23 psychoactive substances were detected at the range of < method detection limits (MDLs) to 24.9 ng L-1. It was found that diazepam and temazepam were the major psychoactive substances in the tap water with the median concentration of 1.0 and 0.06 ng L⁻¹, respectively. The high exposure dose for each psychoactive substance was calculated from 0.6 to 855 pg kg⁻¹ bw d⁻¹ and showed an order of men ≥ boys ≥ girls ≥ women. Risk assessment revealed there was little risk of psychoactive substances on human health at current residual levels.

In order to examine whether 8:2 FTOH exposure would lead to a contamination risk of perfluoroalkyl and polyfluoroalkyl substances (PFASs) in broiler derived food, the biotransformation, and tissue distribution and accumulation of 8:2 FTOH following oral exposure in male broilers were investigated. The main metabolites of 8:2 FTOH in plasma and six tissues (muscle, liver, kidney, fat, heart, and lungs) identified by LC-Q-TOF were 2-perfluorooctyl ethanoic acid (8:2 FTCA), 8:2 fluorotelomer unsaturated carboxylic acid (8:2 FTUCA), 3-perfluoroheptyl propanoic acid (7:3 FTCA), perfluoropentanoic acid (PFPeA), perfluorooctanoic acid (PFOA), perfluoroheptanoic acid (PFHpA), perfluorohexanoic acid (PFHxA), perfluorononanoic acid (PFNA), 8:2 FTOH glucuronide conjugate, and 8:2 FTOH sulfate conjugate. The tissue distribution and bioaccumulation of 8:2 FTOH and its unconjugated metabolites were determinated by LC-MS/MS. 8:2 FTOH was quickly depleted in plasma and all six tested tissues, while PFOA, PFNA, and 7:3 FTCA showed strong accumulation in blood and all six examined tissues and were eliminated more slowly than the other metabolites. The tissues with the highest accumulation levels for 8:2 FTOH and its metabolites were heart, kidneys and liver, and the tissue with the lowest accumulation levels was muscle. The elimination half-lifes of PFNA in kidney and 7:3 FTCA in lung were longer compared to those of other metabolites in all six determined tissues. Thus, PFNA and 7:3 FTCA can be selected as potential biomonitoring markers after 8:2 FTOH exposure. This study has improved our understanding of 8:2 FTOH biotransformation and tissue bioaccumulation in broilers, which will help us monitor human exposure risk via food derived from broilers polluted by 8:2 FTOH.