Mediterranean coastal regions are particularly exposed to oil pollution due to extensive industrialization, urbanization and transport of crude and refined oil to and from refineries. Bioremediation of contaminated beach sand through landfarming is both simple and cost-effective to implement compared to other treatment technologies. The purpose of the present study was to investigate the effect of alternative nutrients on biodegradation of crude oil contaminated beach sand in an effort to reduce the time required for bioremediation employing only indigenous hydrocarbon degraders.A natural sandy soil was collected from Agios Onoufrios beach (Chania, Greece) and was contaminated with weathered crude oil. The indigenous microbial population in the contaminated sand was tested alone (control treatment) or in combination with inorganic nutrients (KNO3 and K2HPO4) to investigate their effects on oil biodegradation rates. In addition, the ability of biosurfactants (rhamnolipids), in the presence of organic nutrients (uric acid and lecithin), to further stimulate biodegradation was investigated in laboratory microcosms over a 45-day period.Biodegradation was tracked by GC/MS analysis of aliphatic and polycyclic aromatic hydrocarbons components and the measured concentrations were corrected for abiotic removal by hopane normalizations. It was found that the saturated fraction of the residual oil is degraded more extensively than the aromatic fraction and the bacterial growth after an incubation period of approximately 3weeks was much greater from the bacterial growth in the control.The results show that the treatments with inorganic or organic nutrients are equally effective over almost 30days where C12–C35n-alkanes were degraded more than 97% and polyaromatic hydrocarbons with two or three rings were degraded more than 95% within 45days. The results clearly show that the addition of nutrients to contaminated beach sand significantly enhanced the activity of indigenous microorganisms, as well as the removal of total recoverable petroleum hydrocarbons (TRPH) over a 45-day study period.

In an attempt to analyze the chemical characterization of oil residues and examine the suitability of chemical fingerprinting methods in oil spill investigations, multiple parameters sensitive to both sources and degree of weathering were used to characterize oil residues from “7–16” Dalian oil spill, China. Oil residues collected 90days to 120days after the spill showed a weathering pattern where significant amounts of light to middle molecular weight normal alkanes were depleted with pristane and phytane as dominant peaks. Diagnostic ratios developed from n-alkane and selected isoprenoids (e.g. Pr/Ph, n-C17/Pr, n-C18/Ph, carbon preference index, LMW/HMW-alkanes ratio), all display obvious changes over weathering time, indicating that these ratios are not valid for oil source identification. Furthermore, the biomarker ratios of hopanes and steranes with relative standard deviations (RSDs) of 0.88–4.08% were useful for source identification even for severely weathered oil residues. In addition, RSD of δ13C values of individual n-alkanes in oil residue varied from 0.07% to 0.20%, which suggest that stable carbon isotope profile of n-alkanes can also be a useful tool for tracing the source of an oil spill.

Macrobenthic intertidal assemblages from five Atlantic Iberian estuaries were analyzed to develop an estuarine index. An R-mode analysis revealed a close association between the isopod Cyathura carinata, the polychaete Hediste diversicolor and the bivalve Scrobicularia plana. Although these species are abundant in all the estuaries considered, they tend to be absent from sites at the marine and freshwater ends of the environmental gradient. Three different ways of calculating the estuarine index are proposed. The index is comprised in the interval [0,1] and was constructed using relative abundances rather than absolute abundances. Transformation of the raw data helped improve the performance of the index. A non-parametric statistical test is proposed for application to the estuarine index to find sites with the same values after a significant omnibus test. The index appears to be a good proxy for recognizing estuarine limits by use of indicator species.

Sediment contamination poses a potential risk for both ecosystems and human health. Risk assessment is troublesome as sediments contain complex mixtures of toxicants, and traditional chemical analyses can neither provide information about potential hazards to organisms nor identify and measure all present contaminants. This work combines the use of the PLHC-1 cell line and the recombinant yeast assay (RYA) to assess the environmental quality of estuarine and coastal sediments. The application of multiple endpoints (cytotoxicity, generation of oxidative stress, presence of CYP1A inducing agents, micronucleus formation and estrogenicity) revealed that the organic extracts of those sediments affected by industrial activities or collected near harbours and untreated urban discharges showed significant cytotoxicity, micronuclei and CYP1A induction. The study highlights the usefulness of the applied bioassays to identify those sediments that could pose risk to aquatic organisms and that require further action to improve their environmental quality.

We investigated the spatial scales of variation of macrofauna in intertidal flats subjected to different levels of contamination from urban effluents in two areas sampled in the Paranaguá Estuarine Complex. The scales considered were: Conditions; Tidal flats and Plots. Although the numerically dominant taxa showed the greatest variability at a scale of Tidal flats, the variability at the Condition scale was also significant. Tubificinae sp. 1, Laeonereis culveri and Heteromastus sp. were the most abundant organisms in the Contaminated area, while Heleobia australis was most abundant in the Non-contaminated area. Our results, contrary to those frequently observed in the literature, showed that the variability was significant at the scale of hundreds of metres (Tidal flats). At this scale, the intrinsic characteristics of each tidal flat are more important in determining macrofaunal distribution, while the effects of the urban sewage contamination represent the primary forces acting at a greater spatial scale.

The Arabian Gulf is considered among the highest anthropogenically impacted regions in the world. Heavy metals contamination in coastal and marine environments is becoming an increasingly serious threat to both the naturally stressed marine ecosystems and humans that rely on marine resources for food, industry and recreation. Heavy metals are introduced to coastal and marine environments through a variety of sources and activities including sewage and industrial effluents, brine discharges, coastal modifications and oil pollution. The present paper reviews heavy metal contamination in a variety of marine organisms, and sediments, and suggests measures for environmental management of heavy metal pollution in the Arabian Gulf. Most of the reviewed literature confirmed that heavy metal concentrations in marine organisms were generally within allowable concentrations and pose no threat to public health. Likewise, studies suggested that levels of heavy metals in marine sediments are similar or lower compared to other regions. However, localized hotspots of chronic metal pollution in areas influenced by industrial facilities, desalination plants, and oil refineries have been reported. Holistic spatial and temporal monitoring and comprehensive national and regional strategies are critical to combat and manage heavy metal pollution in the Arabian Gulf.

Pharmaceuticals are common chemical contaminants in estuaries receiving effluent from wastewater and sewage treatment facilities. The purpose of this research was to examine benthic microalgal (BMA) community responses to sublethal exposures to tylosin, a common and environmentally persistent antibiotic. Bioassays, using concentrations of 0.011–218μmoltylosinl−1, were performed on intertidal muddy sediments from North Inlet Estuary, SC. Exposure to tylosin resulted in a reduction in total BMA biomass and primary productivity. Furthermore, exposure seemed to retard diatom growth while having a minimal effect on cyanobacteria biomass. Estuarine systems receiving chronic inputs of trace concentrations of tylosin, as well as other antibiotics, may experience significant reductions in BMA biomass and primary productivity. Given the well-documented role of BMA in the trophodynamics of estuaries, these impacts will likely be manifested in higher trophic levels with possible impairments of the structure and function of these sensitive systems.

This study focused on the spatial distribution of trace metals in the Veracruz Reef System in the Southern Gulf of Mexico, and its variability in the early (July) and late (September) rainy season of 2008, by analyzing the concentration of Cd, Cu and Pb in benthic macroalgae. Mean concentrations are lower (Pb 295±347ngg−1, Cd 17.9±15.0ngg−1), or similar (Cu 3.4±4.5μgg−1) to those reported from other coastal areas. Cd and Pb concentrations are influenced by the discharge of the Jamapa River, evidencing a fluvial control on coastal trace metal levels. Also, Cd and Cu concentrations were lower in the late rainy season, when there is a high load of suspended sediments derived from fluvial discharge, which probably adsorb dissolved metals decreasing their bioavailability. Pb concentrations have been decreasing in the last two decades in the SGM, after the banning of leaded-gasoline in the late 20th century.

The paper detailed a water quality modelling study of a hyper-tidal estuary, undertaken to assess the impact of various bacteria input loads on the receiving waters in a coastal basin in the UK, by using the model developed in previous study of the same authors enterococci, used as the indicators for bathing water quality under the new European Union (EU) Bathing Water Directive, were numerically modelled using a hydro-environmental model. In particular, the numerical model used in this study includes the effects of sediment on bacteria transport processes in surface water. Finally, the importance of sediment bacteria inputs on the bathing water quality was also investigated under different weather and tidal condition. During spring tide, the bacteria input from the bed sediments are dominant for both wet and dry weather conditions. During neap tides and during dry weather conditions the inputs of bacteria from the bed sediment were still dominant, but during wet weather conditions the inputs from river were dominant. Under different tidal flow conditions some parameters had a more significant role than others. During high flow conditions the sediment re-suspensions processes were dominant, therefore the bed bacteria concentrations played a dominant role on the overall bacteria concentration levels in the water column. In contrast, during low flow conditions sediment deposition prevails and bacteria are removed from the water column. The partition coefficient was found to be more important than the bed bacteria concentrations, during low flow conditions.