The Fort McMurray region in northeastern Alberta (Canada) is rich in natural sources of polycyclic aromatic compounds (PACs) from exposed bitumen beds; anthropogenic sources are being released with increased oil sands industry expansion. Here we report on investigations of PACs (47 compounds) in three species of forage fish collected during the 2012–2013 Joint Oil Sands Monitoring Program (JOSMP) fish health investigations and compare results with PAC data for sediment and water collected under JOSMP and earlier programs. PAC concentrations in sediments varied three orders in magnitude and were highest at downstream tributary mouths, which flowed through the exposed McMurray Formation, and along reaches of the Athabasca River where the formation was exposed. PAC concentrations in water were less variable but with higher concentrations near exposed bitumen beds. Forage fish exhibited the weakest spatial gradients in ΣPACs concentration, which averaged 102 ± 32 ng/g in trout-perch from the Athabasca River, 125 ± 22 ng/g in lake chub from the Ells River, and 278 ± 267 ng/g in slimy sculpin from the Steepbank, Firebag, and Dunkirk Rivers. Low-molecular weight compounds, particularly naphthalenes and fluorenes, dominated fish PACs. Phenanthrenes occurred in greater percent composition in fish caught in areas where PAC concentrations in sediments were higher due to the proximity of bitumen sources than in other areas. Dibenzothiophene, a major component of bitumen PAC, was a minor component of fish ΣPACs. Forage fish PAC concentrations were below fish consumption guidelines established by the European Commission (2011) and for the reopening of the commercial fisheries closed by the Deepwater Horizon oil spill. PAC concentrations in forage fish were similar to concentrations observed in many other studies (fish market surveys, estuaries, and marine waters) and lower than in fish sampled from highly impacted areas (near refineries, harbors, and other industrialized areas).

The objective of this study was to evaluate the potential of three C4 perennial grasses (Miscanthus x giganteus, Panicum virgatum and Spartina pectinata) for biomass production on arable land unsuitable for food crop cultivation due to Pb, Cd and Zn contamination. We assessed soil properties, biomass yield, metal concentrations, and the photosynthetic performance of each species. Physico-chemical and elemental analyses were performed on soil samples before plantation establishment (2014) and after three years of cultivation (2016), when leaf area index, plant height, yield and heavy metal content of biomass were also determined. Physiological measurements (gas exchange, pigment content, chlorophyll a fluorescence) were recorded monthly between June and September on mature plants in 2016. Cultivation of investigated plants resulted in increased pH, nitrogen, and organic matter (OM) content in soil, although OM increase (13%) was significant only for S. pectinata plots. During the most productive months, maximal quantum yield values of primary photochemistry (Fv/Fm) and gas exchange parameter values reflected literature data of those plants grown on uncontaminated sites. Biomass yields of M. x giganteus (15.0 ± 0.4 t d.m. ha−1) and S. pectinata (12.6 ± 1.2 t d.m. ha−1) were also equivalent to data published from uncontaminated land. P. virgatum performed poorly (4.1 ± 0.4 t d.m. ha−1), probably due to unfavourable climatic conditions, although metal uptake in this species was the highest (3.6 times that of M. x giganteus for Pb). Yield and physiological measurements indicated that M. x giganteus and S. pectinata were unaffected by the levels of contamination and therefore offer alternatives for areas where food production is prohibited. The broad cultivatable latitudinal range of these species suggests these results are widely relevant for development of the bioeconomy. We recommend multi-location trials under diverse contaminant and environmental regimes to determine the full potential of these species.

Understanding the mercury (Hg) methylation process is important for the management of paddy soils contaminated by Hg. In this work, samples of eighteen paddy soils with varying soil properties were spiked with inorganic Hg and subjected to a 90 d flooding period. Soil pH and redox potential (Eh) were measured in situ at intervals, and soils were sampled for the analysis of methylmercury (MeHg). The Hg methylation efficiency increased with flooding time and reached a relatively steady state at 30 d of incubation, ranging from 0.08% to 2.52%, and was significantly correlated with the in situ soil pH and Eh. The Elovich equation could adequately describe the kinetic production of MeHg. MeHg production was well predicted by the in situ soil pH and Eh of flooded soils, in addition to the organic matter content of air-dried soil samples and flooding time. The two predictive models explained 78% and 68% of the variability of the Hg methylation efficiency. The results suggested that the methylation of inorganic Hg in paddy soils after flooding can be predicted as a function of routinely measured soil properties and flooding time, a correlation that can be utilized to improve understanding of the extent of Hg methylation and the management of Hg-contaminated paddy soils.

659 water samples from springs and wells in the Sabatini and Vicano-Cimino Volcanic Districts (central Italy) were analyzed for arsenic (As), fluoride (F⁻) and radon (²²²Rn) concentrations. Waters mostly sourced from a shallow and cold aquifer hosted within volcanic rocks, which represents the main public drinking water supply. Cold waters from perched aquifers within sedimentary formations and thermal waters related to a deep hydrothermal reservoir were also analyzed. The highest concentrations of As and F⁻ were measured in the thermal waters and attributed to their enhanced mobility during water-rock interaction processes at hydrothermal temperatures. Relatively high concentrations of As and F⁻ were also recorded in those springs and wells discharging from the volcanic aquifer, whereas waters hosted in the sedimentary units showed significantly lower contents. About 60% (As) and 25% (F⁻) of cold waters from the volcanic aquifer exceeded the maximum allowable concentrations for human consumption. Such anomalously high levels of geogenic pollutants were caused by mixing with fluids upwelling through faulted zones from the hydrothermal reservoir. Chemical weathering of volcanic rocks and groundwater flow path were also considered to contribute to the observed concentrations. Cold waters from the volcanic aquifer showed the highest ²²²Rn concentrations, resulting from the high contents of Rn-generating radionuclides in the volcanic units. Approximately 22% of these waters exceeded the recommended value for human consumption. A specific Quality Index (QI), comprised between 1 (very low) and 4 (very high), was computed for each water on the basis of As, F⁻ and ²²²Rn concentrations and visualized through a spatial distribution map processed by means of geostatistical techniques. This map and the specific As, F⁻ and ²²²Rn maps can be regarded as useful tools for water management by local authorities to both improve intervention plans in contaminated sectors and identify new water resources suitable for human consumption.

Plastic pollution in the marine environment poses threats to wildlife and habitats through varied mechanisms, among which are the transport and transfer to the food web of hazardous substances. Still, very little is known about the metal content of plastic debris and about sorption/desorption processes, especially with respect to weathering. In this study, plastic debris collected from the North Atlantic subtropical gyre was analyzed for trace metals; as a comparison, new packaging materials were also analyzed. Both the new items and plastic debris showed very scattered concentrations. The new items contained significant amounts of trace metals introduced as additives, but globally, metal concentrations were higher in the plastic debris. The results provide evidence that enhanced metal concentrations increase with the plastic state of oxidation for some elements, such as As, Ti, Ni, and Cd. Transmission electron microscopy showed the presence of mineral particles on the surface of the plastic debris. This work demonstrates that marine plastic debris carries complex mixtures of heavy metals. Such materials not only behave as a source of metals resulting from intrinsic plastic additives but also are able to concentrate metals from ocean water as mineral nanoparticles or adsorbed species.

Microcystis aeruginosa is one of the main species of cyanobacteria that causes water blooms. M. aeruginosa can release into the water several types of microcystins (MCs), which are harmful to aquatic organisms and even humans. However, few studies have investigated the hepatotoxicity of M. aeruginosa itself in zebrafish in environments that simulate natural aquatic systems. The objective of this study was to evaluate the hepatotoxicity of M. aeruginosa in adult zebrafish (Danio rerio) after short-term (96 h) exposure and to elucidate the potential underlying mechanisms. Distinct histological changes in the liver, such as enlargement of the peripheral nuclei and sinusoids and the appearance of fibroblasts, were observed in zebrafish grown in M. aeruginosa culture. In addition, antioxidant enzyme activity was activated and protein phosphatase (PP) activity was significantly decreased with increasing microalgal density. A proteomic analysis revealed alterations in a number of protein pathways, including ribosome translation, immune response, energy metabolism and oxidative phosphorylation pathways. Western blot and real-time PCR analyses confirmed the results of the proteomic analysis. All results indicated that M. aeruginosa could disrupt hepatic functions in adult zebrafish, thus highlighting the necessity of ecotoxicity assessments for M. aeruginosa at environmentally relevant densities.

Rorippa globosa (Turcz.) Thell. is known as Cd hyperaccumulator, however neither hyperaccumulation nature, nor affecting factors like the effect of Cd compounds entering soil from different sources, or of specific soil amendments, are not yet satisfactorily clarified. In the pot culture experiment, Cd accumulation by R. globosa from soils spiked with 3 and 9 mg Cd kg⁻¹ in the form of Cd(NO₃)₂, CdCl₂, CdBr₂, CdI₂, CdSO₄, CdF₂, Cd(OH)₂, CdCO₃, Cd₃(PO₄)₂, CdS and effect of soil amendment with glutathione (GSH) were investigated. Accumulation capacity of R. globosa for Cd appeared to reflect its extractability in soils and was about two-fold bigger for high soluble compounds than for low-soluble ones. At that, the differences between the accumulation of Cd originating from high soluble compound group did not exceed 20%, while the differences within the low soluble compound group were insignificant (p < 0.05). The analysis of Cd uptake, uptake factor (UF), enrichment factor (EF) and translocation factor (TF) patterns revealed that Cd hyperaccumulating properties of R. globosa are based on the high water/nutrients demand and strong tolerance to Cd, although weak protection against Cd uptake by root system was also observed. Amendment with GSH enhanced Cd availability to plant and its uptake from soil, but exerted no effect on Cd translocation in plants. In the light of the results, the use of R. globosa for phytoremediation of moderately polluted agricultural lands as forecrop or aftercrop, and the GSH-assisted phytoremediation of highly polluted post-industrial sites seem to be viable options.

Nickel nanoparticles (NiNPs) have an estimated production of ca. 20 tons per year in the US. Nickel has been risk-assessed for long in Europe, but not NiNPs, hence the concern for the environment. In the present study, we focused on investigating the mechanisms of toxicity of NiNPs and the comparison to NiNO3. The high-throughput microarray for the soil ecotox model Enchytraeus crypticus (Oligochaeta) was used. To anchor gene to phenotype effect level, organisms were exposed to reproduction effect concentrations EC20 and EC50, for 3 and 7 days. Results showed commonly affected pathways between NiNPs and NiNO3, including increase in proteolysis, apoptosis and inflammatory response, and interference with the nervous system. Mechanisms unique to NiNO3 were also observed (e.g. glutathione synthesis). No specific mechanisms for NiNPs were found, which could indicate that longer exposure period (>7 days) is required to capture the peak response to NiNPs. A mechanisms scheme is assembled, showing both common and unique mechanisms to NiNO3 and NiNPs, providing an important framework for further, more targeted, studies.

Epidemiological evidence showed that the particulate matter exposure is associated with atherosclerotic plaque progression, which may be related to foam cell formation, but the mechanism is still unknown. The study was aimed to investigate the toxic effects and possible mechanism of PM2.5 on the formation of macrophage foam cells induced by oxidized low density lipoprotein (ox-LDL). Results showed that PM2.5 induced cytotoxicity by decreasing the cell viability and increasing the LDH level in macrophage foam cells. PM2.5 aggravated the lipid accumulation in ox-LDL-stimulated macrophage RAW264.7 within markedly increasing level of intracellular lipid by Oil red O staining. The level of ROS increased obivously after co-exposure to PM2.5 and ox-LDL than single exposure group. In addition, serious mitochondrial damage such as the mitochondrial swelling, cristae rupturing and disappearance were observed in macrophage foam cells. The loss of the mitochondrial membrane potential (MMP) further exacerbated the mitochondrial damage in PM2.5-induced macrophage foam cells. The apoptotic rate increased more severely via up-regulated protein level of Bax, Cyt C, Caspase-9, Caspase-3, and down-regulated that of Bcl-2, indicating that PM2.5 activated the mitochondrial-mediated apoptosis pathway. In summary, our results demonstrated that PM2.5 aggravated the lipid accumulation, mitochondrial damage and apoptosis in macrophage foam cells, suggesting that PM2.5 was a risk factor of atherosclerosis progression.

Radioactive contamination of the natural areas is one of the most long-lasting anthropogenic impacts on the environment. Scots pine (Pinus sylvestris L.) is a promising organism for radiation-related research because of its high radiosensitivity, but the genome size of Pinacea species has imposed obstacles for high-throughput studies so far. In this work, we conducted the analysis of the de novo assembled transcriptome of Scots pine populations growing in the Chernobyl-affected zone, which is still today contaminated with radionuclides because of the accident at the nuclear power plant in 1986. The transcriptome profiles indicate a clear pattern of adaptive stress response, which seems to be dose-dependent. The transcriptional response indicates a continuous modulation of the cellular redox system, enhanced expression of chaperones and histones, along with the control of ions balance. Interestingly, the activity of transposable element families is inversely correlated to the exposure levels to radiation. These adaptive responses, which are triggered by radiation doses 30 times lower than the one accepted as a safe for biota species by international regulations, suggest that the environmental management in radiation protection should be reviewed.