Bacterial communities in antimony (Sb) polluted soils have been well addressed, whereas the important players fungal communities are far less studied to date. Here, we report different responses of bacterial and fungal communities to Sb contamination and the ecological processes controlling their community assembly. Soil samples in the Xikuangshan mining area were collected and subjected to high through-put sequencing of 16S rRNA and ITS1 to investigate bacterial and fungal communities, respectively, along an Sb gradient. Sb speciation in the soil samples and other physicochemical parameters were analyzed as well. Bacterial communities were dominated by Deltaproteobacteria in the soil with highest Sb concentration, whereas Chloroflexi were dominant in the soil with lowest Sb concentration. Fungal communities in high-Sb soils were predominated by unclassified Fungi, whilst Leotiomycetes were dominant in low-Sb soil samples. Multivariate analysis indicated that Sb, pH and soil texture were the main drivers to strongly impact microbial communities. We further identified Sb-resistant microbial groups via correlation analysis. In total, 18 bacterial amplicon sequence variants (ASVs) were found to potentially involve in biogeochemical cycles such as Sb oxidation, sulfur oxidation or nitrate reduction, whereas 12 fungal ASVs were singled out for potential heavy metal resistance and plant growth promotion. Community assembly analysis revealed that variable selection contributed 100% to bacterial community assembly under acidic or high Sb concentration conditions, whereas homogeneous selection dominated fungal community assembly with a contribution over 78.9%. The community assembly of Sb-resistant microorganisms was mainly controlled by stochastic process. The results offer new insights into microbial ecology in Sb-contaminated soils, especially on the different responses of microbial communities under identical environmental stress and the different ecological processes underlining bacterial and fungal community assembly.

Targeting the removal of Pb²⁺ in wastewater, cellulosic materials were carbonized in an aerobic environment and activated via ion exchange. The maximum adsorption capacity reached 243.5 mg/g on an MCC-derived adsorbent activated with sodium acetate. The modified porous properties improved the adsorption capacity. The capacity could be completely recovered five times through elution with EDTA. Because of the negative effects of Ni, Mg, and Ca elements, the adsorption capacities of activated carbonized natural materials were lower than that of pure cellulose. N₂ adsorption measurement showed that the adsorbent had a large specific surface area as well as abundant micropores and 4-nm-sized mesopores. FTIR and surface potential results proved that carboxyl group was generated in the aerobic carbonization, and was deprotonated during ion exchange. This adsorbent consisted of C–C bonds as the building blocks and hydrophilic groups on the surface. XPS results demonstrated that the Pb 4f binding energies were reduced by 0.7–0.8 eV due to the interaction between Pb²⁺ and the activated adsorbent, indicating that the carboxylate groups bonded with Pb²⁺ through coordination interactions. Pseudo-second-order and Elovich kinetic models were well fitted with the adsorption processes on the pristine and activated carbonized adsorbents, indicative of chemisorption on heterogeneous surfaces. The Freundlich expression agreed well with the data measured, and the pristine and activated adsorbents had weak and strong affinities for Pb²⁺, respectively. The Pb²⁺ adsorption process was exothermic and spontaneous, and heat release determined the spontaneity. The adsorption capacity is attributed to the carboxylate groups and pores generated in the aerobic oxidation and ion exchange procedures.

The soil environment serves as an assembling area for microplastics, and is an important secondary source of microplastics in other environmental media. Recently, soil microplastics have been extensively studied; however, high variability is observed among the research results owing to different soil properties, and the complexity of soil microplastic composition. The present study amassed the findings of 2886 experimental groups, across 38 studies from 2016 to 2022, and used meta-analysis to quantitatively analyze the differences in the effects of microplastic exposure on soil physicochemical properties and biota. The results showed that among the existing soil microplastic research, agricultural soils maintained a higher environmental exposure distribution than other environments. Microplastic fibers and fragments were the predominant shapes, indicating that the extensive use of agricultural films are the primary influencing factor of soil microplastic pollution at present. The results of the meta-analysis found that microplastic exposure had a significant negative effect on soil bulk density (lnRR = −0.04) and aggregate stability (lnRR = −0.085), indicating that microplastics may damage the integrity of soil structure or damage the soil surface. The significant changes in plant root biomass and soil phosphatase further signified the potential impact of microplastics on soil nutrient and geochemical element cycling. We further constructed species sensitivity distribution curves, revealing that invertebrates had a higher species sensitivity to microplastics, as they can pass through the gut wall of soil nematodes, causing oxidative stress and affecting gene expression. In general, soil is an interconnected complex, and microplastic exposure can directly or indirectly interact with environmental chemical processes in the soil environment, potentially harming the soil ecosystem; however, current research remains insufficient with respect to breadth and depth in terms of the comprehensive “source-sink” mechanism of soil microplastics, the hazard of exposure, and the overall toxic effects.

Chinese cabbage (Brassica rapa ssp. pekinensis) is one of the most popular and frequently consumed leafy vegetables. It was found that atmospheric PM₂.₅-Pb contributes to Pb accumulation in the edible leaves of Chinese cabbage via stomata in North China during haze seasons with high concentrations of fine particulate matter in autumn and winter. However, it is unclear whether both stomata and trichomes co-regulate foliar transfer of PM₂.₅-Pb from atmospheric deposition to the leaf of Chinese cabbage genotypes with trichomes. Field and hydroponic experiments were conducted to investigate the effects of foliar uptake of PM₂.₅-Pb on Pb accumulation in leaves using two genotypes of Chinese cabbage, one without trichomes and one with trichomes. It was verified that open stoma is a prominent pathway of foliar PM₂.₅-Pb transfer in the short-term exposure for 6 h, contributing 74.5% of Pb accumulation in leaves, whereas Pb concentrations in the leaves of with-trichome genotype in the rosette stage were 6.52- and 1.04-fold higher than that of without-trichome genotype in greenhouse and open field, respectively, which suggests that stomata and trichomes co-regulate foliar Pb uptake of from atmospheric PM₂.₅. Moreover, subcellular Pb in the leaves was distributed in the following order of cytoplasm (53.8%) > cell wall (38.5%)> organelle (7.8%), as confirmed through high-resolution secondary ion mass spectrometry (NanoSIMS). The Leadmium™ Green AM dye manifested that Pb in PM₂.₅ entered cellular space of trichomes and accumulated in the basal compartment, enhancing foliar Pb uptake in the edible leaves of cabbage. The results of these experiments are evidence that both stomata and trichomes are important pathways in the regulation of foliar Pb uptake and translocation in Chinese cabbage.

Impacts of tropospheric ozone on sweet potato (Ipomoea batatas) are poorly understood despite being a staple food grown in locations deemed at risk from ozone pollution. Three varieties of sweet potato were exposed to ozone treatments (peaks of: 30 (Low), 80 (Medium), and 110 (High) ppb) using heated solardomes. Weekly measurements of stomatal conductance (gs) and chlorophyll content (CI) were used to determine physiological responses, along with final yield. gs and CI were reduced with increasing ozone exposure, but effects were partially masked due to elevated leaf senescence and turnover. Yield for the Erato orange and Murasaki varieties was reduced by ∼40% and ∼50% (Medium and High ozone treatments, respectively, vs Low) whereas Beauregard yield was reduced by 58% in both. The DO₃SE (Deposition of Ozone for Stomatal Exchange) model was parameterized for gs in response to light, temperature, vapour pressure deficit and soil water potential. Clear responses of gs to the environmental parameters were found. Yield reductions were correlated with both concentration based AOT40 (accumulated ozone above a threshold of 40 ppb) and flux based POD₆ (accumulated stomatal flux of ozone above a threshold of 6 nmol m⁻ ² s⁻ ¹) metrics (R² 0.66 p = 0.01; and R² 0.44 p = 0.05, respectively). A critical level estimate of a POD₆ of 3 (mmol m⁻² Projected Leaf Area⁻¹) was obtained using the relationship. This study showed that sweet potato yield was reduced by ozone pollution, and that stomatal conductance and chlorophyll content were also affected. Results from this study can improve model predictions of ozone impacts on sweet potato together with associated ozone risk assessments for tropical countries.

Next-generation sequencing method employing the sphingomonads-specific and universal 16s rRNA primers were applied to analyze the response of soil bacterial community to continuous stress of nanoscale zero-valent iron (nZVI) and/or polycyclic aromatic hydrocarbons (PAHs) under suspended conditions. The group specific primer greatly improves taxonomic resolution, the overall OTU numbers increased from 12 by universal primer to 42 by sphingomonads-specific primer. It was found that nZVI and PAHs had opposite selection effects on bacterial community composition, furthermore, the abundance of Sphingopyxis was positively related to nZVI while negatively related to PAHs, whereas abundances of Sphingobium and Sphingosinicella were positively related to PAHs (p < 0.01). The improved taxonomic resolution helps identify the potential keystone taxa, which provides valuable information for future bioremediation technology such as synthetic microbiome.

Sewage sludges are rich in organic matter and several essential nutrients for plant growth, making them very appealing for application in agricultural soils. However, they may also contain a wide range of emerging pollutants, which has raised concerns about the potential risks of this practice to crops, the environment, and public health - accumulation in soils and/or plant uptake and translocation of contaminants. Therefore, there is a need to study plant-soil interactions and assess the uptake potential of these contaminants by food crops to better understand these risks. The main aim of this work was to assess the possible drawbacks of sludge application to cropland, by observing the impact on the growth and yield of a model crop (pea plant - Pisum sativum) grown over an 86-day greenhouse experiment and by assessing the uptake potential of synthetic musk fragrances. Different sewage sludge application rates (4–30-ton ha⁻¹) and initial concentrations of contaminants were tested. The application of sludge yielded benefits to the cultivated plants, finding improved crop productivity with an application rate of 30-ton ha⁻¹. At the end of the experiment, soil samples and plants separated into sections were analysed using a QuEChERS extraction methodology followed by gas chromatography-mass spectrometry (GC-MS) quantification. Galaxolide (HHCB) and tonalide (AHTN) underwent uptake by the plant roots, having been detected in concentrations up to 346 ng g⁻¹ on a dry weight basis (dw), but only HHCB was detected in above ground tissues. At the end, a decrease in the levels of synthetic musks in the amended soils (>80% in several instances) was observed. Assuming the worst-case scenario, no risk to human health was observed from the ingestion of peas grown on sewage sludge-amended soils. However, a soil hazard quotient analysis yielded worryingly high quotient values for AHTN in nearly all tested conditions.

Although the hazards of microplastics (MPs) have been quite well explored, the aberrant metabolism and the involvement of the autophagy pathway as an adverse response to environmental MPs in benthic organisms are still unclear. The present work aims to assess the impact of different environmental MPs collected from the south coast of the Mediterranean Sea, composed by polyethylene (PE), polyethylene vinyl acetate (PEVA), low-density polyethylene (LDPE), high-density polyethylene (HDPE), polypropylene (PP) and polyamide (PA) on the metabolome and proteome of the marine polychaete Hediste diversicolor. As a result, all the microplastic types were detected with Raman microspectroscopy in polychaetes tissues, causing cytoskeleton damage and induced autophagy pathway manifested by immunohistochemical labeling of specific targeted proteins, through Tubulin (Tub), Microtubule-associated protein light chain 3 (LC3), and p62 (also named Sequestosome 1). Metabolomics was conducted to further investigate the metabolic alterations induced by the environmental MPs-mixture in polychaetes. A total of 28 metabolites were differentially expressed between control and MPs-treated polychaetes, which showed elevated levels of amino acids, glucose, ATP/ADP, osmolytes, glutathione, choline and phosphocholine, and reduced concentration of aspartate. These novel findings extend our understanding given the toxicity of environmental microplastics and unravel their underlying mechanisms.

Land application of sewage sludge is increasingly used as an alternative to landfilling and incineration owing to a considerable content of carbon and essential plant nutrients in sewage sludge. However, the presence of chemical and biological contaminants in sewage sludge poses potential dangers; therefore, sewage sludge must be suitably treated before being applied to soils. The most common methods include anaerobic digestion, aerobic composting, lime stabilization, incineration, and pyrolysis. These methods aim at stabilizing sewage sludge, to eliminate its potential environmental pollution and restore its agronomic value. To achieve best results on land, a comprehensive understanding of the transformation of organic matter, nutrients, and contaminants during these sewage-sludge treatments is essential; however, this information is still lacking. This review aims to fill this knowledge gap by presenting various approaches to treat sewage sludge, transformation processes of some major nutrients and pollutants during treatment, and potential impacts on soils. Despite these treatments, overtime there are still some potential risks of land application of treated sewage sludge. Potentially toxic substances remain the main concern regarding the reuse of treated sewage sludge on land. Therefore, further treatment may be applied, and long-term field studies are warranted, to prevent possible adverse effects of treated sewage sludge on the ecosystem and human health and enable its land application.