Feammox is a newly discovered and important anaerobic nitrogen (N) loss pathway, and its variation and role in removing N following the application of N fertilizer and its migration from paddies to other land use types and from surface soils to deep soils have not been thoroughly elucidated to date. In this study, field sampling and slurry incubation experiments were performed to evaluate the Feammox rate between different land use types (paddy, irrigation ditch, riparian zone and lake, 0–10 cm) and different paddy soil depths (0–70 cm) in a wheat-rice rotation area in China. Based on a ¹⁵N-labelled isotope-tracing technique and analysis of microbial communities, it was estimated that the potential Feammox rate ranged from 0.031 to 0.42 mg N kg⁻¹ d⁻¹ in this area. In the soil profile of the paddy, the depth of 20–30 cm was the active region of Feammox, with a value of 0.37 ± 0.057 mg N kg⁻¹ d⁻¹. Compared with the surface soil (0–10 cm) of the paddy (0.18 ± 0.031 mg N kg⁻¹ d⁻¹), the potential Feammox rate of the irrigation ditch soil was not significantly different, but that of the lake riparian soil and lake sediment were decreased by 27.27% and 32.11%, respectively (p < 0.01). Fe(III) content was the best predictor of the Feammox rate and explained the variation of the Feammox rate by 36.00% in the surface soil. At the genus level, the paddy soil at a depth of 20–30 cm had the greatest abundance of the genera in which the Fe reduction bacteria were distributed; and where Bacillus, Geobacter and Anaeromyxobacter had higher proportions. It was estimated that the potential N loss by Feammox was in the range of 7.36 (the lake) ∼43.35 (the paddy) kg N ha⁻¹ year⁻¹ in the surface soil of this area. Considering denitrification and the Feammox rate as a whole, we found that denitrification remained to be the main contributor to N loss in the surface soil (94.72–96.89% of N loss), although Feammox dominated N loss in the deep soil (below 0–10 cm).

Little information is available about effect of particle size and crystal structure of nTiO₂ on their trophic transfer. In this study, 5 nm anatase, 10 nm anatase, 100 nm anatase, 20 nm P25 (80% anatase and 20% rutile), and 25 nm rutile nTiO₂ were selected to investigate the effects of size and crystal structure on the toxicity, bioconcentration, and trophic transfer of nTiO₂ to algae and daphnia. In the exposed daphnids, metabolic pathways affected by nTiO₂ and nTiO₂-exposed algae (nTiO₂-algae) were also explored. The 96 h IC₅₀ values of algae and the 48 h LC₅₀ values of daphnia were 10.3, 18.9, 43.9, 33.6, 65.4 mg/L and 10.5, 13.2, 37.0, 28.4, 60.7 mg/L, respectively, after exposed to nTiO₂-5A, nTiO₂-10A, nTiO₂-100A, nTiO₂-P25, and nTiO₂-25R, respectively. The bioconcentration factors (BCFs) for 0.1, 1, and 10 mg/L nTiO₂ in daphnia ranged from 21,220 L/kg to 145,350 L/kg. The nTiO₂ biomagnification factors (BMFs) of daphnia fed with 1 and 10 mg/L nTiO₂-exposed algae were consistently greater than 1.0 (5.7–122). The results show that the acute toxicity, BCF, and BMF all decreased with increasing size or rutile content of nTiO₂. All types of nTiO₂ were largely accumulated in the daphnia gut and were not completely depurated within 24 h. At the molecular level, 22 Kyoto Encyclopedia of Genes and Genomes (KEGG) metabolic pathways of daphnia were impacted by the nTiO₂ and nTiO₂-algae treatments, including glutathione metabolism, aminoacyl-tRNA biosynthesis, among others. Six and four KEGG metabolic pathways were significantly disturbed in daphnids exposed to nTiO₂ and nTiO₂-algae, respectively, indicating the presence of algae partially alleviated the negative impact of nTiO₂ on metabolism. These findings increase understanding of the impacts of physicochemical properties of nTiO₂ on the food chain from molecular scale to that of the whole organism, and provide new insight into the ecological effect of nanomaterials.

Maternal exposure to phthalates may cause some adverse health effects on both mother and fetus, but variations of phthalate exposure and metabolism during pregnancy have not been thoroughly characterized. A total of 946 participants were selected from a cohort study conducted in Wuhan between 2014 and 2015 through which they had provided a complete set of urine samples at three trimesters. Eight phthalate metabolites were analyzed in 2838 urine samples. Based on urinary concentrations, various parameters (i.e. phthalate metabolite concentrations, ratios of metabolites of bis(2-ethylhexyl) phthalate (DEHP) in DEHP, and percentages of individual metabolites in total phthalates) were compared over three visits. We observed that levels of phthalate metabolites showed a U-shaped trend across three trimesters. The significant variations in the ratios of DEHP metabolites indicated that the efficiency in metabolizing DEHP declined during pregnancy and less recent exposure occurred in mid-pregnancy. The changes of percentages of individual compound in total phthalates suggested the inconsistent pattern over trimesters. This longitudinal study found that the exposure pattern, exposure timing and metabolic susceptibility varied by trimesters, which suggests that urine samples should be collected at multiple time points and mothers should be especially careful in the early pregnancy.

Chemical mixtures represent environmentally-realistic exposures of contaminants to aquatic biota. However, there remains a limited understanding of how toxicant mixtures may impact biological function, relative to their individual components. In the current study, oxidative stress responses of the freshwater galaxiid fish inanga (Galaxias maculatus) were examined following exposure to the pro-oxidant trace metal cadmium (2 or 9 μg L⁻¹), and the anti-oxidant pharmaceutical drug diclofenac (770 μg L⁻¹), individually or in simple binary mixtures. Cadmium exposure in the absence of diclofenac significantly decreased renal catalase activity, increased hepatic catalase activity, decreased renal superoxide dismutase (SOD) and decreased glutathione-S-transferase activity, effects that are suggestive of anti-oxidant defense inhibition and/or generation of increased reactive oxygen species. Diclofenac exposure in the absence of cadmium resulted in a decreased renal lipid peroxidation, consistent with its known anti-oxidant properties. The presence of waterborne diclofenac altered the effects of cadmium on catalase activity in the liver, SOD activity in the gill, and lipid peroxidation in the liver. Co-exposure with cadmium modulated diclofenac effects on lipid peroxidation in the kidney. These data indicate the capacity of each of these toxicants to offset biological effects of the other when both co-occur in urban waters at specific concentrations. This study also demonstrates the complexity of outcomes in contaminant mixtures, even when these stressors are presented as simple binary combinations.

There is growing recognition of a potential role for environmental and climatic factors in influencing cardiovascular risk. It has been speculated that temperature variability (TV) is a risk factor for cardiac arrhythmia but evidence is limited.To quantify the geographic and demographic variations in the association between TV and hospitalization for cardiac arrhythmia in Brazil during 2000–2015.Data on hospitalization for arrhythmia and weather conditions were collected from 1,814 cities. TV was calculated as the standard deviation of daily maximum and minimum temperatures during exposure days. A time-stratified case-crossover approach was applied to examine the city-specific association between TV and hospitalization for arrhythmia. City-specific estimates were pooled at the national and regional levels using a random-effect meta-analysis. Stratified analyses were conducted by sex, three age-groups (0–64, 65–74 and ≥75 years), and three arrhythmia subtypes (paroxysmal tachycardia, atrial fibrillation and flutter, and other arrhythmias).There were 447,667 arrhythmia-related hospitalizations during 2000–2015. The odds ratio of hospitalization per 1 °C increase in TV peaked on 0–1 days’ exposure [1.012 (95% confidence interval: 1.010–1.015)]. There were no substantial differences in effect estimates of TV₀₋₁ by region, age or sex, except for the non-significant association observed in the north. However, women were more affected by prolonged TV exposure than men. For the three arrhythmias subtypes, only paroxysmal tachycardia and other arrhythmias were sensitive to TV. Assuming a causal relationship, 35,813 (95%CI: 18,302−51,665) cases were attributable to TV₀₋₁ in Brazil during 2000–2015, accounting for 8.0% (95%CI: 4.1–11.5%) of hospitalizations for cardiac arrhythmia.At a population-level exposure to TV was associated with increased risk of arrhythmia-related hospitalization in Brazil, with the relationship equally distributed across most residents but varied by arrhythmia subtypes. Our findings add to the accumulating evidence-base that climatic factors can influence cardiovascular outcomes in populations.

Atmospheric stability significantly influences the accumulation and dispersion of air pollutants in the near-surface atmosphere, yet few stability metrics have been applied as predictors in statistical PM₂.₅ concentration mapping practices. In this study, eleven stability metrics were derived from radiosonde soundings collected in eastern China for the time period of 2015–2018 and then applied as independent predictors to explore their potential in favoring the prediction of PM₂.₅. The statistical results show that the in situ PM₂.₅ concentration measurements correlated well with these stability metrics, especially at monthly and seasonal timescales. In contrast, correlations at the daily timescale differed markedly between stability metric and also varied with seasons. Nevertheless, the modeling results indicate that incorporating these stability metrics into the PM₂.₅ modeling framework rendered small contribution to PM₂.₅ prediction accuracy, yielding an increase of R² by < 5% and a reduction of RMSE by < 1 μg/m³ on average. Compared with other stability indices, the inversion depth and intensity appeared to have relative larger benefiting potential. In general, our findings indicate that including these stability metrics would not result in significant contribution to the PM₂.₅ prediction accuracy in eastern China since their effects could be partially overwhelmed or offset by other predictors such as AOD and boundary layer height.

Recent studies have demonstrated the occurrence of microplastic fibers (MFs) in soil environments. To determine whether MFs are harmful for soil biota, we evaluated toxic effects on terrestrial snails (Achatina fulica) after 28 d exposure to polyethylene terephthalate MFs at concentrations of 0.01–0.71 g kg−1 (dry soil weight). Digestion kinetics experiments on 24 snails showed that MFs can be ingested and excreted within 48 h. We found the appearance of cracks and deterioration on the surface of MFs after depuration by the digestive system. Prolonged exposure to 40 snails showed that 0.14–0.71 g kg−1 MFs caused an average reduction of 24.7–34.9% food intake and 46.6–69.7% excretion. 0.71 g kg−1 MFs induced significant villi damage in the gastrointestinal walls of 40% snails, but did not influence the histology of the liver and kidney. Moreover, 0.71 g kg−1 MFs exposure reduced glutathione peroxidase (59.3 ± 13.8%) and total antioxidant capacity (36.7 ± 8.5%), but elevated malondialdehyde level (58.0 ± 6.4%) in the liver, which indicates oxidative stress is involved in the toxic mechanism. Our results suggest that MFs have adverse impacts on the fitness of soil organisms, and highlight the ecological risks of microplastic pollution in terrestrial ecosystems.

We have previously reported on the ubiquitous presence of perchlorate in the deposited and airborne fine dusts of Malta and shown that the source of the chemical in the dusts of this small central Mediterranean island is fireworks. There are no local geologic or anthropogenic sources of perchlorate other than firework manufacture and display. The hypothesis was tested that ground-deposited perchlorate will be mobilized in runoff and would partly migrate to the water table and eventually also affect tap water, one third of which being derived from groundwater. Forty four percent of 36 groundwater samples contained perchlorate above detection limit with mean and median values of 1.09 and 1.1 μg L−1. Sixty-two percent of 16 runoff samples collected during storms contained perchlorate above detection limit with mean and maximum concentrations, respectively, of 50.8 and 129 μg L−1, values which are far too high to be explained by atmospheric inputs given that rainwater perchlorate levels are typically <3 μg L−1. Between 42 and 89% of the tap waters analyzed in three sampling campaigns contained perchlorate above detection limit and had mean concentrations ranging from 0.4 to 1.6 μg L−1 suggesting contamination levels similar to those reported from China but lower than levels reported from the USA. The phenomenon of contamination of the water resources of Malta by perchlorate is probably unique in that it results not from geologic or industrial inputs but from an intense and prolonged pyrotechnic activity that is deeply rooted in the popular culture of the islanders.

A novel carbonaceous material (NCM), prepared by the pyrolyzation of the oily sludge of tank bottom, was proposed to remove Cr(VI) from a synthetic solution for the first time. The effects of initial Cr(VI) concentration, NCM dosage and initial solution pH on Cr(VI) removal and the adsorption kinetics, the adsorption isothem were investigated. The removal mechanism was studied by comparing the surface properties of NCM before and after the Cr(VI) removal. The results showed that NCM can effectively remove Cr(Ⅵ) from the synthetic solution with the increase of solution pH at equilibrium. At the initial Cr(Ⅵ) concentrations of 40, 100, 150 and 250 mg/L and NCM dosages of 1, 3, 6 and 8 g/L with initial solution pH of 2, the removal efficiency of Cr(VI) was 95.5, 96.8, 95.2 and 81.2%, and the solution pH at equilibrium reached 2.3, 3.5, 5.8 and 7.5, respectively. NCM was suitable for Cr(Ⅵ) removal while the initial Cr(VI) concentration was less than 100 mg/L and initial solution pH was lower than 2.5. Most of Cr(VI) was removed by the reduction of Fe2+ and S2− in NCM to Cr(III) and with the generation of stable FeCr2O4. Some Cr(VI) may be removed by reacting with Fe2+ and Ca2+ to produce CaCrO4 and FeCrO4 on the NCM surface. The dissolution of CaAl2Si2O8 and CaS in the solution increased the solution pH at equilibrium. NCM has been proved to be a material with dual functions both chemical reduction and adsorption.

Ambient particulate matter (PM) exposure is associated with pulmonary and cardiovascular diseases; however, there is scant research linking data on animal and human cells. The objective of this study was to investigate these associations. Vascular remodeling plays a crucial role in both pulmonary and cardiovascular diseases. Therefore, we conducted a transcriptomic analysis using vascular smooth muscle cells (VSMCs) to identify potential regulators or markers of PM exposure. We demonstrated that fine and coarse PM increased VSMC proliferation in mice. We conducted a genome-wide cDNA microarray analysis, followed by a pathway analysis of VSMCs treated with coarse PM for durations of 24, 48, and 72 h. Sixteen genes were discovered to be time-dependently upregulated and involved in VSMC proliferation. Osteopontin (OPN) is indicated as one of the regulators of these upregulated genes. Both fine and coarse PM from industrial and urban areas significantly increased OPN expression in VSMCs and macrophages. Moreover, oropharyngeal instillation of fine and coarse PM for 8 weeks increased the VSMCs in the pulmonary arteries of mice. OPN level was consistently increased in the lung tissues, bronchoalveolar lavage fluid, and serum of mice. Moreover, we analyzed the plasma OPN levels of 72 healthy participants recruited from the studied metropolitan area. Each participant wore a personal PM2.5 sampler to assess their PM2.5 exposure over a 24 h period. Our results indicate that personal exposure to fine PM is positively correlated with plasma OPN level in young adults. The data obtained in this study suggest that exposure to fine and coarse PM may cause pulmonary vascular lesions in humans and that OPN level may be a biomarker of PM exposure in humans.