Nanoplastics can be used in various fields, such as personal care products. Nevertheless, the effect of nanoplastic exposure on metabolism and its association with stress response remain largely unclear. Using Caenorhabditis elegans as an animal model, we determined the effect of nanopolystyrene exposure on lipid metabolism and its association with the response to nanopolystyrene. Exposure (from L1-larave to adult day-3) to 100 nm nanopolystyrene (≥1 μg/L) induced severe lipid accumulation and increase in expressions of mdt-15 and sbp-1 encoding two lipid metabolic sensors. Meanwhile, we found that SBP-1 acted downstream of intestinal MDT-15 during the control of response to nanopolystyrene. Intestinal transcriptional factor SBP-1 activated two downstream targets, fatty acyl CoA desaturase FAT-6 and heat-shock protein HSP-4 (a marker of endoplasmic reticulum unfolded protein response (ER UPR)) to regulate nanopolystyrene toxicity. Both MDT-15 and SBP-1 were involved in the activation of ER-UPR in nanopolystyrene exposed nematodes. Moreover, SBP-1 regulated the innate immune response by activating FAT-6 in nanopolystyrene exposed nematodes. In the intestine, function of MDT-15 and SBP-1 in regulating nanopolystyrene toxicity was under the control of upstream signaling cascade (PMK-1-SKN-1) in p38 MAPK signaling pathway. Therefore, our data raised an important molecular basis for potential protective function of lipid metabolic response in nanopolystyrene exposed nematodes.

Pharmaceutically active compounds (PhACs) have attracted increasing attention due to their large consumption volumes, high bioactivity and potential ecotoxicity. In this study, a total of 150 commonly used drugs were investigated in sediments of Jiaozhou Bay (JZB). Twenty-five target compounds were detected, of which ten were discovered for the first time in marine sediments. The range of total PhAC content was 3.62–21.4 ng/g dry weight. Ketoprofen (2.49 ng/g), oxytetracycline (1.00 ng/g) and roxithromycin (0.97 ng/g) were the preponderant PhACs. PhACs gradually decreased from east to west, and the distribution of PhACs in the sediment was controlled by the source channel, seawater dynamic process and sediment composition. The diatom, organic matter, and clay proportions in the sediments and the nutrients in the overlying water were the most important environmental factors affecting the distribution of PhACs. PhAC pollution in the sediments of the JZB exhibited an increasing trend. Coprostanol could be used as a chemical indicator of the PhAC concentration in JZB sediments. PhACs were mainly derived from direct pollution due to human fecal excretion in the eastern region. Ofloxacin, tetracycline and oxytetracycline were found to pose high or medium risks to aquatic organisms. It is necessary and urgent to improve the treatment technology of drug residues in sewage treatment plants to decrease the pollution of PhAC residues. With the continuous aging of the global population, the use of PhACs will increase rapidly, which may cause more unpredictable threats to the marine ecosystem. Therefore, the monitoring of PhACs in the marine environment needs to be strengthened, and studies on PhAC occurrence and effects must be considered a priority in global environmental research.

Thallium (Tl) is a dispersed trace metal showing remarkable toxicity. Various anthropogenic activities may generate Tl contamination in river sediments, posing tremendous risks to aquatic life and human health. This paper aimed to provide insight into the vertical distribution, risk assessment and source tracing of Tl and other potentially toxic elements (PTEs) (lead, cadmium, zinc and copper) in three representative sediment cores from a riverine catchment impacted by multiple anthropogenic activities (such as steel-making and Pb–Zn smelting). The results showed high accumulations of Tl combined with associated PTEs in the depth profiles. Calculations according to three risk assessment methods by enrichment factor (EF), geoaccumulation index (Igₑₒ) and the potential ecological risk index (PERI) all indicated a significant contamination by Tl in all the sediments. Furthermore, lead isotopes were analyzed to fingerprint the contamination sources and to calculate their quantitative contributions to the sediments using the IsoSource software. The results indicated that a steel-making plant was the most important contamination source (∼56%), followed by a Pb–Zn smelter (∼20%). The natural parental bedrock was found to contribute ∼24%. The findings highlight the importance of including multiple anthropogenic sources for quantitative fingerprinting of Tl and related metals by the lead isotopic approach in complicated environmental systems.

Reservoirs are lentic man-made waterbodies resulting from river damming processes. Pollutants coming from adjacent areas can accumulate in the water and sediment of these modified freshwater environments. Fish are often found in reservoirs occupying several trophic niches. Biochemical biomarkers are early warning signals of environmental disturbance to an organism. It is essential to understand how pollutants, abiotic variables and biochemical biomarker responses behave throughout the seasons to implement biomonitoring programs. Loricariichthys anus and Geophagus brasiliensis were collected, and abiotic variables were seasonally measured for one year, at six sampling sites in Passo Real reservoir, in a subtropical region of Southern Brazil. Biochemical biomarkers were analyzed in four tissues of both fish species, as well as metal and pesticide concentrations in the reservoir’s water and sediment. Redundancy analysis (RDA) was carried out to find the temporal relationship between biomarkers and environmental variables. RDA has clearly shown the separation of seasons for both species. Azoxystrobin, simazine and propoxur were the pesticides mostly contributing to the variation, whereas metals had lesser contribution to it. Seasonality appears to be the main factor explaining biomarkers’ variability. PERMANOVA has confirmed the effect of temperature and dissolved oxygen on biomarkers of both fish species. Thus, it is hard to differentiate if the fluctuation in biomarkers’ responses only reflects the normal state of organisms or it is a biological consequence from negative effects of fish exposure to several types of pollution (sewage, pesticides, and fertilizers) entering this aquatic system. In this study, to circumvent the seasonality issue on biomonitoring, the analysis of biomarkers on these fish should not be carried out in organs directly affected by temperature (such as liver and gills), or during reproduction periods (mainly in Spring).

The trace elements contamination of agricultural soils near petrochemical industry complexes is a concern due to the risk of accumulating in food systems and subsequently affecting human health. We measured representative trace elements (Cu, Ni, Cr, Pb, Zn, Pb, Hg and As) through the soil-plant (maize)-human contamination pathway near a petrochemical industry complexes in an agricultural region from September 20 to 28, 2016. We found that the soil was mildly to moderately polluted by multiple trace elements, which was also confirmed by the contamination factor and enrichment factor values. Cd (enrichment factor = 2.28), Cu (2.75), Zn (1.85) and Pb (1.70) should be given more attention and prioritized over the other trace elements due to their higher potential risks. Furthermore, the trace elements contamination in maize grains was lower than the corresponding limits. The sequence of the transfer coefficient values was Zn > Cd > Cu > Hg > Ni > As > Cr > Pb. Maize grain safety was threatened mainly by Zn, Cd and Cu. There was no risk to humans through soil ingestion, while a potential health risk from maize grain consumption existed. Children were more sensitive than adults to the non-carcinogenic risks of maize grain consumption. Trace element As was found to be the priority metal for risk control. For carcinogenic risk, adults were more sensitive than children; As, Cr and Cd were the priority metals for risk control, with CRₘₐᵢzₑ values exceeding the risk threshold (1 × 10⁻⁴). Overall, strict, intensive monitoring, especially of Cr and Cd, and soil protection measures are needed to prevent any furthertrace elements contamination and to ensure food safety. This study also provides a reference for similar studies worldwide.

Civilization development is associated with the use of plastic. When plastic was introduced to the market, it was assumed that it was less toxic than glass. Recently, it is known that plastics are serious ecological problem they, do not degrade and remain in the environment for hundreds of years.Plastic may be degraded into micro-particles < 5000 nm in diameter, and further into nanoparticles (NPs) < 100 nm in diameter. NPs have been detected in air, soil, water and sludge.One of the most commonly used plastics is polystyrene (PS) - a product of polymerization of styrene monomers. It is used for the production of styrofoam and other products like toys, CDs and cup covers. In vivo and in vitro studies have suggested that polystyrene nanoparticles (PS-NPs) may penetrate organisms through several routes i.e. skin, respiratory and digestive tracts. They can be deposited in living organisms and accumulate further along the food chain. NPs are surrounded by “protein corona” that allows them penetrating cellular membranes and interacting with cellular structures. Depending on the cell type, NPs may be transported through pinocytosis, phagocytosis, or be transported passively. Currently there are no studies that would indicate a carcinogenic potential of PS-NPs. On the other hand, the PS monomer (styrene) was classified by the International Agency for Research on Cancer (IARC) as a potentially carcinogenic substance (carcinogenicity class B2).Despite of the widespread use of plastics and the presence of plastic NPs of secondary or primary nature, there are no studies that would assess the effect of those substances on human organism. This study was aimed at the review of the literature data concerning the formation of PS-NPs in the environment, their accumulation along the food chain, and their potential adverse effects on organisms on living various organization levels.

Due to extensive mining and industrial activities, arsenic (As) and antimony (Sb) contaminations are becoming a global environmental concern. Both As and Sb are toxic and carcinogenic metalloids from the group 15 in the periodic table. Since As and Sb share many similar geochemical properties, it is often assumed that they exert similar environmental pressure on the native microbial communities. This hypothesis, however, still requires further confirmation. In the current study, a systematic comparison of microbial responses to As and Sb contamination were conducted. The results suggested that regular geochemical parameters, such as pH, nitrate, and TOC, were the driving forces for shaping the microbial community. In correspondence, two heavily contaminated groups showed similar microbial community compositions and the same microbial populations were enriched. The interactions between the contaminant fractions (As and Sb related fractions) and the individual OTUs, however, suggested the different and more diverse impacts of As comparing to Sb fractions, with more taxa significantly impacted by As species comparing to Sb species. The identification of the keystone taxa in the heavily contaminated samples revealed a group of microbial populations that could survive in both As and Sb heavily contaminated conditions and may providing critical environmental services to the community. Further investigation of these key microbial populations may provide valuable insights on employing these microorganisms for remediation applications.

The non-steroidal anti-inflammatory drugs (NSAIDs) ibuprofen and diclofenac are highly prescribed worldwide and their presence in aquatic system may pose a potential risk to aquatic organisms. Here, we systematically assessed their cardiovascular disruptive effects in zebrafish (Danio rerio) at environmentally relevant concentrations between 0.04 and 25.0 μg/L. Ibuprofen significantly increased the cardiac outputs of zebrafish embryos at actual concentrations of 0.91, 4.3 and 21.9 μg/L. It up-regulated the blood cell velocity, total blood flow and down-regulated the blood cell density at concentrations of 4.3 μg/L and higher. In comparison, diclofenac led to inhibition of spontaneous muscle contractions and decreased hatching rate of zebrafish embryos at the highest concentration (24.1 μg/L), while it had negligible effects on the cardiac physiology and hemodynamics. Transcriptional analysis of biomarker genes involved in cardiovascular physiology, such as the significantly up-regulated nppa and nkx2.5 expressions response to ibuprofen but not to diclofenac, is consistent with these observations. In addition, both ibuprofen and diclofenac altered the morphology of intersegmental vessels at high concentrations. Our results revealed unexpected cardiovascular functional alterations of NSAIDs to fish at environmental or slightly higher than surface water concentrations and thus provided novel insights into the understanding of their potential environmental risks.

Cadmium (Cd) is a toxic heavy metal and widespread in environment and food, which is adverse to human and animal health. Food intervention is a hot topic because it has no side effects. Selenium (Se) is an essential trace element, found in various fruits and vegetables. Many previous papers have described that Se showed ameliorative effects against Cd. However, the underlying mechanism of antagonistic effect of Se against Cd-induced cytotoxicity in avian leghorn male hepatoma (LMH) cells is unknown, the molecular mechanism of Se antagonistic effect on Cd-induced and calcium (Ca²⁺) homeostasis disorder and crosstalk of ER stress and autophagy remain to be explored. In order to confirm the antagonistic effect of Se on Cd-induced LMH cell toxicity, LMH cells were treated with CdCl₂ (2.5 μM) and Na₂SeO₃ (1.25 and 2.5 μM) for 24 h. In this study, Cd exposure induced cell death, disrupted intracellular Ca²⁺ homeostasis and Ca²⁺ homeostasis related regulatory factors, interfered with the cycle of cadherin (CNX)/calreticulin (CRT), and triggered ER stress and autophagy. Se intervention inhibited Cd-induced LDH release and crosstalk of ER stress and autophagy via regulating intracellular Ca²⁺ homeostasis. Moreover, Se mitigated Cd-induced Intracellular Ca²⁺ overload by Ca²⁺/calmodulin (CaM)/calmodulin kinase IV (CaMK-IV) signaling pathway. Herein, CNX/CRT cycle played a critical role for the protective effect of Se on Cd-induced hepatotoxicity. Based on these findings, we demonstrated that the application of Se is beneficial for prevention and alleviation of Cd toxicity.

The high frequency of acid rain in southern China has captured public and official concern since 1980s. Subsequently, gas emission reduction measures have been implemented to improve the air quality. Variations in SO₂ emission intensities can influence the sulfur and oxygen isotopic compositions of sulfate in rainwater, since atmospheric sulfate is mainly formed via the oxidation of sulfur gases from natural and anthropogenic sources. To evaluate the impacts of emission reduction measures on atmospheric sulfate, the seasonal and long-term trends in stable isotopic compositions of sulfate in rainwater in Guizhou province, southwestern China have been investigated based on rainwater samples collected from June 2016 to June 2018 and literature investigation (2000–2010).The results reveal that coal combustion remains a major contributor to sulfate in rainwater, although its SO₂ emission has significantly decreased over the past two decades. The δ³⁴Sₛᵤₗfₐₜₑ and δ¹⁸Oₛᵤₗfₐₜₑ values in rainwater are negatively correlated and have significant seasonal changes. The seasonality in δ³⁴Sₛᵤₗfₐₜₑ has been interpreted as due to the changes in contributions of dimethyl sulfide and coal combustion, while the seasonal pattern of δ¹⁸Oₛᵤₗfₐₜₑ is consistent with that of δ¹⁸Owₐₜₑᵣ values, indicating sulfate in rainwater is mainly formed by heterogeneous oxidation of SO₂. Combined with the data from previous studies (Xiao and Liu, 2002; Liu, 2007; Xiao et al., 2009; Xiao et al., 2014), we found that the volume weighted mean δ³⁴S values of sulfate in rainwater in Guizhou province show a marked increase between 2001 and 2018, indicating that the ³⁴S-depleted SO₂ emission from coal combustion has declined during this period. Furthermore, the synchronous changes in δ³⁴S values, sulfate concentration and pH values of rainwater suggest that the frequency of acid rain in Guizhou province has dropped over the past two decades, which is likely to result from the emission reduction measures taken in Guizhou province.