Studies have been conducted to find out the effect of the feeding of calves (Bos Taurus) with Jerusalem artichoke (Helianthus tuberosus) concentrate produced in Latvia containing the prebiotic – inulin. The study was performed on two groups of animals - a control group of 8 animals and test (prebiotic) group of 8 animals in summer (from June to August, 2013), and winter (from December, 2013 to February, 2014) in one of cow farm of Latvia, in the municipality of Bauska. Both groups were fed the whole milk, but the test group received additionally 12 g of Jerusalem artichoke powder (an average of 500 g kgE-1 inulin) per day. The overall health status and physiological parameters (temperature, heartbeat and breathing frequency) of both animal groups before the study were of the normal range. After the experiment, we found out that the calves of the test group during both winter (one case) and summer seasons (seven cases), had fewer cases of diarrhea than the control (winter months four cases and summer months nine cases) group animals, the average daily weight gain (control group 0.53 g, prebiotic group 0.75 g) and the total weight gain (control group 29.42 kg, prebiotic group 42.13 kg) during 56 test days was significantly higher ( p is less than 0.05) than that for the control animals. We concluded that the use of Jerusalem artichoke flour concentrate when fed to the calves generally gives positive impact on the development and growth of the calves, improves the status of the gastrointestinal tract and the morphometric indicators.

This article presents the results of studies of the chemical composition of the fish meat while using as a feed additive zeolite of Chankanay origin. The research was conducted during 2011-2013 at the Department of Veterinary-sanitary examination and hygiene of the Kazakh National Agrarian University and at the laboratory of JSC ‘Kazakh Academy of Nutrition’ LTD ‘NUTRITEST’. The material of study was the zeolitic tuff of Chankanay deposit (Almaty region) and two-year trout Oncorhynchus mykiss. For the research we used feed by recipes of GosNIORH - 12-80. The chemical composition of fish meat was determined by a set of methods: moisture by drying at 105 °C, fats by Soxhlet, total protein by modified Kjeldahl method (ISO 937:1974), and minerals by incineration in a muffle furnace. Calorie content of meat was determined by Alexandrov’s formula: X = C - (F+A) ´ 4.1 + F ´ 9.3. Fatty acid composition of fish meat was determined by gas-liquid chromatography. Thus, the results are the indirect evidence that zeolites added to primary diet for growing fish, do not adversely impact proteolytic enzyme systems of fish, that is, have no negative effect on fish organism.

Nutritional value and shelf life of milk and dairy products depend on the composition and stability of their constituents. The aim of the present study was to evaluate the effects of carrots as cow feed carotenoid source on butter oil fatty acid (FA) composition and oxidative stability. Milk was obtained from one trial group (TG; n=5) and one control cow group (CG; n=5) in a conventional dairy farm in Latvia. TG cows received carrots 7 kg per cow per day; the length of the supplementation period was 39 days. The stability of butter oil exposed to sunlight (3h) and held for 14 days in the temperature of 60 °C was analyzed by peroxide value method. The changes of the FA content and ratios in TG milk fat were more positive with respect to the fat nutritional value as observed in CG – stronger increase in the content of polyunsaturated FA (p is less than 0.05), and in the ratio between stearic and palmitic acids (p is less than 0.05); also a tendency was seen of increasing content of short and monounsaturated FA, as well as decreasing the ratio between ω6 and ω3 FA groups. Oxidative stability of the carrot supplemented cow group’s milk butter oil samples that were collected after 25- day trial period, exposed to sunlight (3h) and stored at a temperature of 60 °C was significantly (p is less than 0.05) higher compared to CG samples. After the 39-day long trial period stability difference was not significant, despite the tendency that average polyunsaturated FA content in TG samples was higher compared to the control.

The purpose of this study was to evaluate the effect of probiotics, herbals and buckwheat bran (Fagopyrum esculentum L.) on growth, profile of blood, gut microbiota, profile of fatty acid in meat and shedding of resistant Escherichia coli (E. coli) in piglets. A total of 44 piglets (Sus scrofa domesticus) from age of day 14 to 56 were divided into 4 groups. Control received basal diet (group C), basal diet + probiotics (group P), basal diet + 3% buckwheat bran (group PB) and basal diet + 1.5% herbals (group H). No effect was observed in growth in all groups. The count of Lactobacillus spp. increased (p is less than 0.05) in jejunum in group P. In the faeces, Enterobacteriaceae decreased in the group P (p is less than 0.05) of 35 days old piglets, but Enterobacteriaceae and E. coli decreased in the group H (p is less than 0.05) of 56 days old piglets. The prevalence of resistance to at least one antibiotic class was 66.7% before and 50% after the experiment in all groups. Multidrug resistance of E. coli was not observed in 14 days old piglets, but was observed in 50% and more in all of study groups of 56 days old piglets. The fatty acid composition of Longissimus thoracis muscle had higher levels of α-linolenic acid and palmitoleic acid (p is less than 0.05), but lower level of stearic acid (p is less than 0.05) in group P. In conclusion, probiotics and herbals improved gut microbiota, fatty acid profile and affected shedding of resistant E. coli, but not growth performance.

Ruminants produce a large amount of methane (CH4 ) and carbon dioxide (CO2 ) in their foregut. These gases cause greenhouse effect. There are a lot of studies about different feed additives which can reduce the production of greenhouse gases in ruminants. Prebiotics can also change the amount of bacteria in animal gastrointestinal tract and reduce the occurrence of diarrhoea. The aim of this study was to test whether the prebiotic inulin affects the production of CH4 and CO2 in calves’ rumen and whether it affects the bacteria count in the rumen fluid and bacterial overgrowth in intestines. We used the flour of Jerusalem artichoke (Helianthus tuberosus L.) containing 50% of inulin. Approximately fifty days old, Holstein Friesian crossbreed calves were used in this study. Eight were in the control group, 8 received 12 g of flour and 8 received 24 g per day. On the 28th and 56th day of the research, we measured the amount of CH4 and CO2 in calves’ rumen took rumen fluid samples for bacterial analysis and urine to measure the level of phenol and indican. We concluded that adding the flour of Jerusalem artichoke at doses 12 g and 24 g did not significantly impact the production of CH4 and CO2 in calves’ rumen, the prebiotic inulin may suppress the growth of anaerobic microorganisms in the rumen at concentration 12 g of inulin reaching 56th day of experiment. The amount of phenol and indican in calves’ morning urine did not correlate with the faecal consistency of calves.

A study was conducted to determine efficiency of an organic acids and phytogenic additives on pigs' growth processes and digestive tract microflora. The feed of the control group (C) did not contain the additives. The feed of the organic acid group (A) contained 6 kg of organic acids additive per tonne feed for pigs after weaning, 4 kg per tonne feed for grower pigs, and 3 kg per tonne feed for finished pigs. The feed of the phytogenic additive group (F) contained 0.5% of phytogenic additive per tonne feed for pigs after weaning, and 0.2% per tonne feed for grower and finished pigs. The feed of organic acids plus phytogenic additives group (A+F) contained 6 kg of organic acids additive per tonne feed for pigs after weaning, 4 kg per tonne feed for grower pigs, and 3 kg per tonne feed for finished pigs 0.5% of phytogenic additive per tonne feed for pigs after weaning, and 0.2% per tonne feed for grower and finished pigs. Compared to groups pigs from the group (F) had by 9.7%, group (A+F) - by 8.3 % and from group (A) had by 3.2% higher mass. The bests results of feed conversion were found in the group (A+F) - 2.84 kg, which was by 8.5% less than in the group (C), whereas the group (F) had by 5.1% and group (A) - by 6.7% better results than the control group. The results of Duodenum and Rectum microflora analyses showed that use of organic acid and phytogenic additives reduced mould yeast Staphylococcus sp. colony formed units (CFU gE-1).

A study was conducted to determine efficiency of an organic acids additive on pigs metabolic processes and diggestive tract microflora. The pigs of control group were fed without an organic acids additive. The feed of the trial group piglets contained 6 kg of organic acids additive per tonne feed, for starter pigs - 4 kg per tonne feed, for finished pigs - 3 kg per tonne feed. The study indicated that at the age of 130 days, pig mass in the trial group was 54.08 kg an average, but in the control group - 51.06 kg, which showed that pigs from the trial group had by 6% higher average mass than in the control group (p is less than 0.005). At the age of 160 days, pig mass in the trial group was an average 94.3 kg, in the control group - 90.63 kg; pigs from the trial group had by 4% higher mass than in the control group (p is less than 0.005). Feed consumption for the trial group was by 1% less than in the control group. Average daily gain for the trial group was 0.663 kg, which was by 6% more than for the control group pigs (p is less than 0.005). Feed conversion in the trial group was 3.06 kg, but in the control group - 3.275 kg, i.e. 6.4% higher than in the trial group. Analyses of Jejunum microflora showed that use of acidifier additive reduced Mould colony formed units - CFU amount in 1 g of the trial group sample by 6%. Staphylococcus species amount in the trial group was 500 CFU in 1 g of sample, but in the control group - 40000 CFU, i.e. 80 times more. In the trial group, Lactic acid bacteria CFU amount in 1 g of sample was by 6% higher than in the control group. The results of Rectum microflora analyses showed that Staphylococcus species amount in the trial group was 12000 CFU in 1 g of sample, but in the control group it was by 34% higher.

A study was conducted to determine efficiency of a phytoadditive on pig growth processes and digestive tract microflora. The pigs of control group were fed without the phytoadditive. The feed of the trial group piglets contained 0.5% of the phytoadditive per tonne feed, for starter pigs and finished pigs - 0.2% per tonne feed. The study indicated that at the age of 170 days, pig mass in the trial group was 111.67+-1.22 kg on average, but in the control group - 101.79+-0.81 kg, which showed that pigs from the trial group had by 9.7% higher average mass than in the control group (p is less than 0.05). Average daily gain for the trial group was 0.777+-0.009 kg, which was by 12 % more than for the control group pigs (p is less than 0.05). Feed conversion in the trial group was 2.928 kg, but in the control group - 3.129 kg, which was by 6.4% higher than in the trial group. Gastric microflora analyses showed that use of phytoadditive reduced mould colony forming units (CFU) amount in the trial group decreased by 24 times. Duodenum microflora analyses showed that use of phytoadditive reduced mould CFU amount by 25%, yeast CFU amount by 34%, Escherichia coli mesophilic and termophilic forms CFU by 16.3% compared to the control. A lactic acid bacterium CFU in the trial group was 2.5 times higher compared to the control. Rectum microflora analyses showed that use of phytoadditive reduced mould CFU amount by 31.6%, yeast CFU amount - by 62%, Escherichia coli mesophilic and termophilic forms by 57 % and 15.6 % respectively. Lactic acid bacteria CFU amount in 1 g of sample in the trial group increased by 5.1 times.

Blood haematology is one of the indicators that shows if a goat (Capra hircus) kid gets a sufficient quantity of liquid, has a good supply of oxygen in the body, as well as an indication of inflammatory processes. Our aim of this research was to find out how feed additives (probiotics (Enterococcus faecium), prebiotics (Jerusalem artichoke (Helianthus tuberosus L.)) and symbiotics (Enterococcus faecium+ Jerusalem artichoke)) influence on haematological parameters of kids. Research was performed in Latvia in 2014. The blood samples were analyzed for leucocytes (WBC), erythrocyte number (RBC), haemoglobin (HGB) concentration, packed cell volume (HCT), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH) and mean corpuscular haemoglobin concentrations (MCHC). Since the blood indicators have not significantly changed in all examination times, we analyzed the results of the age of 4, 8 and 12 weeks. HCT downward trend in blood sample MRG + PRO, MRG + PRE and MRG + SIM group of kids was observed from the fourth week up to the eighth week and then it gradually increased. The results showed that the HCT, HGB, RBC, MCHC and WBC number was comparable (p is greater than 0.05) between groups. Our results prove that the use of Jerusalem artichoke powder as an additive to food not only improves digestion and metabolism of ruminant, but also helps to maintain constant haematological values. In the control group and the kids who received probiotics, prebiotics or symbiotics - significant differences are evident in all haematological parameters (p is less than 0.05) at the age of 12 weeks.