The study was aimed to investigate the effect of potato (Solanum tuberosum L.) in vitro plantlets planting density under greenhouse conditions on obtained minitubers number per unit area, multiplication rate and their weight distribution. Three cultivars of different maturity (‘Monta’ – early maturity, ‘Prelma’ – medium early and ‘Mandaga’ – medium late maturity) were used for the study. In vitro plantlets were planted in a greenhouse of State Priekuli Plant Breeding Institute, Latvia in 2014 at four planting densities (PDs) 63 plants per square m, 95 plants per square m, 142 plants per m2 and 184 plants per square m respectively. Fertilized peat was used as a substratum. Significant effect of planting density (p is less than 0.001) and cultivar (p is less than 0.01) was found on analyzed yield parameters. Increased planting densities resulted in increased minitubers number per square m (from 272 minutubers mE-2 at PD 63 plants mE-2 to 414 minutubers mE-2 at PD 184 plants mE-2), decreased multiplication rate (4.3 to 2.7 minitubers per planted plant) and mean fresh weight of minitubers (from 20.26. g to 12.11 g). The highest increase of minitubers number per m2 was observed within size (weight) range 3 to 5 g. Minitubers number per m2 increase within bigger size ranges (5 to 10 g, 10 to 20 g) was less pronounced. Slight insignificant (p=0.330) decrease of minitubers number is greater than 20 g was observed in relation of planting density increase (112 minitubers mE-2 at PD 95 plants mE-2 to 84 minitubers mE-2 at PD 184 plants mE-2).