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PCR-identification of mycobacteria isolated from tuberculin-reacting cattle in Estonia
2001
Sudakov, M. | Suett, S. | Kumar, J. | Haekkinen, L. (Estonian Agricultural Univ., Tartu (Estonia). Agrobiocentre)
However, the last case of tuberculosis to cattle was registered in Estonia in 1986, rapid and accurate diagnosting of the disease is still an actual veterinary problem. On farms, tuberculin testing has been regularly carried out showing that tuberculin positive cattle constitute 5,47% from all the herd. During 1997-2001, 23 clinical samples were isolated from the tuberculin positive cattle. Each sample was analysed with conventional morphological and bacteriological methods and by PCR. None of eleven mycobacterial cultures, isolated from the lymph modes of cattle, belonged to M. tuberculosis complex. There were seven cultures identified as M. avium and one as M. avium complex (MAC). The research confirmed that M. avium is the most frequently isolated bacteria causing NTM-Mycobacterioses in cattle in Estonia (63,6%). Two cultures of MAC were isolated from the milk of tuberculin-positive cows, verifying that milk can be a potential source of MAC transmission to man.
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