Hyperadrenocorticism (excessive adrenal production of sex steroids related to adrenocortical adenomas and carcinomas) is a common disease in neutered male and female pet ferrets (Mustela putorius furo) and causes significant morbidity. Incidence of ferret adrenal disease in Latvia is high because the majority of them are gonadectomized. The most common clinical symptoms include symmetrical alopecia and recurrence of sexual behavior. Sex steroids used to diagnose disease are estradiol, androstenedione and 17α-OH progesterone. It is mentioned that androstenedione is the most sensitive during development of the disease. The aim of this study was to sum up, evaluate and compare clinical findings and blood hormone measurements in ferrets diagnosed with hyperadrenocorticism. Forty two ferrets (n = 20 female, n = 22 male) with various clinical symptoms related to hyperadrenocorticism were included in this research. Clinical examination based on clinical symptom protocol was performed and blood samples were taken in all cases. There is a high correlation between the age of neutering and onset of clinical signs in female ferrets. In both sexes, there is no correlation between clinical signs and levels of sex steroids. In male and female group, symmetrical alopecia, pruritus, scaling and fragility of skin and lethargy are the most common findings. During this research, a ranking system (from 1 to 4) for the severity of alopecia was developed. All ferrets had one or two elevated sex steroid levels. In our research, androstenedione was elevated in 25 cases out of 42. Clinical signs and sex steroid measurements are important in diagnosing hyperadrenocorticism in ferrets.

Parafasciolopsosis is a parasitological disease which is caused by the liver fluke Parafasciolopsis fasciolaemorpha. This parasite which belongs to herbivores often causes invasion in elks (Alces alces). The aim of our study was to diagnose the parasite and investigate what kind of histopathological changes it caused in the liver tissue. The study took place in Latvia University of Life Sciences and Technologies, Faculty of Veterinary Medicine, Laboratory of Comparative Pathology and Laboratory of Parasitology. During the study from 2017 to 2018 we collected liver samples and faeces from 46 felled elks of different age and gender, from all over Latvia. Ten grams of faeces were examined for trematode eggs by sedimentation method and a section of liver was examined for the presence of flukes and the histological structure of the liver. In this study Parafasciolopsis fasciolaemorpha were found in 11 samples of liver from 46 elks, with invasion extensity of 24%. The histopathological examination of the liver samples revealed fibrosis, proliferation of bile ducts, pericholangitis, calcerous deposits in bile ducts and other notable histological changes in the liver tissue that can cause hepatic trematodes.

The aim of our study was to describe the histopathological and cytological characteristic of the cow endometrium on the seventh day of the oestrous cycle. In this study, 11 different breeds’ dairy cows (78.18 ± 37.46 months old, in 3.6 ± 2.17 lactation, the mean body condition score 3.4 ± 0.72 (5 points scale)) from Research and Study farm ‘Vecauce’ were selected. All cows were more than 210 days postpartum. Overall health and reproductive tract examination was performed, progesterone (P4) and estradiol (E2) concentration in blood serum were established and the biopsy and cytology samples of endometrium were taken. Mean E2 concentration was 14.92 ± 7.92 pg mLE−1, mean P4 concentration was 13.64 ± 9.44 nmol LE−1. The mean percentage in the cytology slides was established: epithelial cells 89 ± 9%, polimorphonuclear leukocytes (PMN) 6 ± 5%. Cytological subclinical endometritis (SE) was confirmed in 5 cows. Histopathological findings (out of 22 samples): endometrium stromal edema in 14, hemosiderin and hemosiderophages in 8, supranuclear vacuolization in 12, pseudodecidual reaction in 12 samples. No subnuclear vacuolization and mitosis in the glandular epithelium were detected. Histopathological examination did not reveal SE. Morphology between the uterine horns with and without corpus luteum (CL) and between cows with serum P4 level higher than 15 nmol LE−1 and lower than 15 nmol LE−1 were not statistically different (p is greater than 0.05). In conclusion, histopatological examination is more reliable diagnostic method for SE. Future investigation should be performed to establish cut-off values for the diagnosis of SE in cows more than 210 days postpartum.

The preservation of local animal breeds is a topical theme in recent years. Latvian Brown (LB) and Latvian Blue (LZ) cow breeds are exhausting and must be preserved. It can be performed by the use of multiple ovulation and embryo transfer (MOET). The difficulties exist in choosing the gene-fond (GF) cows as donors caused by the small number of animals. Twenty-three cows were intended for donor cow’s role from different herds until September 2018, and twenty of them were accepted. Anamnesis, clinical examination, blood morphology (13 parameters) and biochemical indices (19 parameters) were analysed before MO induction. Three cows were rejected because of ovarian cysts or pyometra, negative energy balance (NEB) (glucose is less than 2.3 mmol LE−1 simultaneously with elevated ß-hydroxybutyric acid is greater than 1.4 mmol LE−1) and elevated (p is less than 0.05) number of leukocytes (28.20 x109 LE−1). More than 52.6% of cows had a repeated artificial insemination before the last parturition, and 5.3% of cows had lifeless offspring in the last parturition. The 1st and 2nd lactation cows were healthier than older cows (p is less than 0.05). The amount of albumins, cholesterol, triglycerides and Na, K, P, Cl, Mg was significantly different in donors with and without successfully obtained embryos (p is less than 0.05). In conclusion, not only acceptable clinical health but also the cow metabolic status is a decisive factor for success of MOET.