The research was performed at the Faculty of Veterinary Medicine of the Latvia University of Agriculture. At the moment the research in veterinary anaesthesia is in its infancy stage. The aim of this study was to determine the effects of the acepromazine maleate on intraocular pressure (IOP) and horizontal pupil diameter (HPD) in a dog's eye. Ten adult dogs of different age, breed and sex were examined clinically and ophthalmologically. All animals were clinically and ophthalmologically healthy. Animals received acepromazine intramuscularly. IOP and HPD were measured every 5 minutes for the total period of 60 minutes. It was established that the acepromazine maleate intramuscular injection causes an IOP decrease in booth eyes. A significant IOP decrease was observed from 35 till 60 minutes after the acepromazine injection. The pupil contraction was observed 5 minutes after the treatment and continued to contract till the end of the research. As acepromazine maleate causes a significant decrease of intraocular pressure, it is not contraindicated to be used in the case of corneal trauma, perforation, glaucoma and corneal ulcers.

This study was performed to detect morphological changes of the gastric mucosa according to the prevalence of helicobacters in the gastric mucosa of domestic dogs (Canis familiaris) from the Small animal clinic of Faculty of Veterinary Medicine, Latvia within 2008 year period. Mucosal samples were taken from several places of cardiac, fundic and pyloric gland region of the stomach to detect helicobacters with light histological examination. Seventeen dogs of eighteen sampled animals showed presence of tightly spiraled helicobacters. Compared to gastric regions our study showed significantly higher prevalence of helicobacters in the cardiac and fundic gland region than in the less effected pyloric gland region of the stomach. Histological examination also showed the depth of location of helicobacters in gastric pits and deeper glandular epithelium of the gastric mucosa in different regions of the stomach. In the pyloric gland region helicobacters are located deeper in the gastric pits and glandular epithelium than in the gastric mucosa of cardiac and fundic gland region Morphological changes of gastric mucosa was divided into 4 groups: 1) mucosa without morphological changes, 2) mucosal hyperemia, 3) mucosal erosions, and 4) mucosal polips. Morphological changes especially mucosal polips are significantly more in the pyloric gland region than in the cardiac and fundic gland region. The amount of morphological changes in the helicobacter positive and negative samples were not significantly different in the cardiac and fundic gland region, but in the pyloric gland region morphological changes of the gastric mucosa are significantly more in the helicobacter positive samples than in the helicobacter negative samples.

This study was done to evaluate the prevalence and colonization density of helicobacters in the gastric mucosa of domestic dogs (Canis familiaris). Mucosal samples were taken from several places of cardiac, fundic and pyloric gland region of the stomach to detect helicobacters with urease test, brush cytology, and light histological examination. Fourteen dogs of fifteen sampled animals showed positive urease test and the presence of tightly spiraled helicobacters. Positive urease test was observed in 80.4% of all examined samples. Helicobacters were detected in 82.7% of all examined samples by brush cytology and 80.8% of all examined samples by histological examination. Compared to gastric regions, in all employed detection methods have been showed significantly higher prevalence of helicobacters in cardiac and fundic gland region than in the less effected pyloric gland region of the stomach. Histological examination showed the location and colonization density of the bacteria at the superficial epithelium, gastric pits, and deep glandular epithelium of the gastric mucosa in different regions of the stomach. Colonization density of bacteria was evaluated in 4 groups: as absent, mild, moderate, and severe colonization density of helicobacters. Occurrence of helicobacters in the cardiac and fundic gland region showed mild and moderate colonization density of helicobacters at the superficial epithelium and at the gastric pits of the gastric mucosa. Colonization density of spiral-shaped bacteria between the deep glandular epithelium was more pronounced in the pyloric gland region than in the cardiac and fundic gland regions of the gastric mucosa in the domestic dogs.

The objective of this study was to determine the effects of topical 1% atropine sulphate and systemic atropine sulphate on intraocular pressure (IOP) and horizontal pupil diameter (HPD) in dog’s eyes. Ten adult dogs for each treatment were used in this study. Dogs of different age, breed and sex were examined clinically and ophthalmologicaly. All animals were clinically and ophthalmologically healthy. One drop of topical 1% atropine sulphate was used in ten dogs unilaterally, with the contralateral eye acting as a control. IOP and HPD were measured every 5 minutes. In ten dogs systemic atropine sulphate were used intramuscularly (IM) with IOP and HPD measured every 5 minutes. In both study phases IOP and HPD were measured over a total duration of 60 minutes. After unilateral application of topical atropine, IOP increased significantly in the treated eye. A maximum average IOP of 20.3 mmHg in the treated eye was observed 20 minutes after treatment. Maximal pupil dilatation in the treated eye was observed 35 min after treatment. Measurements made after systemic atropine showed an IOP increase in both eyes, showing maximum average IOP increase 25 minutes post-treatment. Maximum average values of HPD were obtained 25 minutes after treatment. The HPD started to decrease 30 minutes after treatment but it was still significantly higher than before treatment (P is less than 0.05). Because of atropine sulphate’s ability to cause significant increase in IOP, it should not be used for diagnosis and treatment of glaucomatous eyes.

Nowadays excessively used corticosteroids in veterinary medicine induce steroid hepatopathy in dogs (Canis lupus familiaris). The objective of this study was to determine the possibility of the hepatoprotectants to decrease the corticosteroid-induced alteration in such dogs’ blood serum enzymes as alaninaminotransferase (ALAT), gammaglutamyltranferase (GGT), alkaline phosphatase (AP) and corticosteroid-induced thermostable alkaline phosphatase (cAP). The study took place in private veterinary clinics in Riga, Latvia, during 2013, with the permission of dogs’ owners. Twenty eight animals, which received corticosteroids due to present diagnosis, were divided into two groups. In the first group long-lasting corticosteroid methylprednisolone acetate injection was used once, while in the second group the hepatoprotectants were used after the injection of corticosteroids. It was discovered that after 14 and after 30 days of hepatoprotectants use, blood enzymes were significantly lower (p is less than 0.05) than in dogs that did not receive hepatoprotectants. In both groups the enzyme values did not reach the reference limits. The study is set to investigate further if and when the values reach the reference limits.

The objective of this study was to observe and evaluate the ability of innovative vascular prosthesis, made in Riga Technical University (RTU), to incorporate in a canine model. The research has been performed in Veterinary Medicine Faculty of Latvia University of Agriculture since July 19. 2011. The research was approved by the Food and Veterinary Service of the Republic of Latvia. The research is realized within the framework of European Social Fund co-financed project ‘Establishment of interdisciplinary research groups for new functional properties of smart textiles development and integrating in innovative products’ (ESF No 2009/0198/1DP/1.1.1.2.0./09/APIA/VIAA/148). RTU produced aortic grafts 5-8 mm in diameter and 8 mm to 18 mm long were implanted retroperitoneal in 9 female, 1-3 years old Beagle dogs. Once before and regularly after the surgery, abdominal aortic and vascular graft diameter was measured in cross sections. Blood pressure was measured before and regularly after the procedure. Results show no significant differences in aortic and graft diameter before and 12 months after the operation and there are no significant differences in systolic and diastolic blood pressure before and 6 months after the aortic surgery at a significance level α 0.05. The study shows that the innovative aortic prostheses do not change in diameter and abdominal aorta transplantation surgery does not cause significant variations in blood pressure. Study is continued to find out later reactions to synthetic vascular graft.

The present study describes situation of antimicrobial resistance of animal pathogens and resistance trends in Estonia in years 2006-2009. Bacterial strains isolated during period 2006-2009 were Escherichia coli (E. coli), Enterococcus faecium (E. faecium), Enterococcus faecalis (E. faecalis), collected from healthy pigs faeces as well as from diagnostic submissions of pig samples. Staphylococcus aureus (S. aureus) isolates originated from cows with clinical mastitis and Staphylococcus pseudointermedius (S. pseudointermedius) isolates from dogs with pyoderma or otitis externa. Antimicrobial susceptibility was detected by microdilution method. Normal enteric microflora from health y pigs had resistance against streptomycin, tetracyclin, sulfametoxazol and trimethoprim. E. faecalis and E. faecium were resistant to erythromycin, tetracyclin, streptomycin and kanamycin. Multiresistance occured mainly against kanamycin, streptomycin and tetracyclin. E. coli strains isolated from pathological material showed high resistance to ampicillin, tetracycline, streptomycin, sulphonamides and trimethoprim. Multiresistance was detected between 60–73% during study years. In 2009, one ESBL (extended spectrum betalactamase) producing isolate was observed. S. aureus strains isolated from clinical mastitis samples were mainly penicillin resistant (58–86%). Meticillinresistant S. aureus was not found during the study. In 2009, resistance to lincomycin (30%) and fucidinic acid (22%) was detected. In S. pseudointermedius strains isolated from canine skin samples the prevalence of resistance to penicillin as high as 53–81% was found. Multidrug resistance was relatively stable being 38% in 2006, 29% in 2007 and 25% in 2009. In conclusion, antimicrobial resistance of animal pathogens in Estonia was high. Further improvement of prudent use of antimicrobials and infection control is needed.

The aim of this study was to find out the biointegration of innovative vascular prosthesis, made in Riga Technical University (RTU), in dog’s abdominal aorta. The research is being performed in Veterinary Medicine Faculty of Latvia University of Agriculture since July 19. 2011. We used 9 female gender and 1-3 years old Beagle dogs in this study. The research is approved by the Latvian Republic Food and Veterinary Service. The study is carried out in the frame of European Social Fund co-financed project ‘Establishment of interdisciplinary research groups for a new functional properties of smart textiles development and integrating in innovative products’ (ESF No. 2009/0198/1DP/1.1.1.2.0./09/APIA/VIAA/148). 5-8 mm in diameter, 8 mm - 18 mm in length RTU produced aortic grafts were implanted retroperitoneal in dogs. Before the surgery the diameter of abdominal aorta was measured in cross sections, afterwards – cranial and caudal from the prosthesis, as well cross-section diameter of vascular graft was measured. Blood pressure was measured before the surgery and after the procedure on regular basis. Results are the following: there are no significant differences in aortic and graft diameter before and 1-2 months after the operation, there are no significant differences in systolic and diastolic blood pressure before and four months after the surgery at significance level α = 0.05. The study shows that the innovative aortic prostheses don’t change in diameter, and surgery like abdominal aorta transplantation doesn’t cause significant variations in blood pressure. The study is being continued to find out later reactions to synthetic vascular graft.

A prospective study was performed on 15 client-owned dogs to assess the clinical outcome after surgical excision of canine cutaneous mast cell tumors (MCTs) depending on histologic grade and completeness of surgical margins. The surgical margins were evaluated as complete, close or incomplete if they were more than 3 mm, from 1 to 3 mm or less than 1 mm wide, respectively. Survival time for dogs with low grade MCT (based on grading by M. Kiupel et al.) was 409 days compared with only 60 days for dogs with high grade tumor. Dogs with high grade tumors had significantly shorter survival time and worse prognosis than dogs with low grade tumors (p=0.013). Complete excision was associated with lower possibility and longer time to tumor recurrence, as well as longer survival, however, marginal surgical border status did not have a significant impact on time to tumor recurrence and survival. It was also found that tumor duration but not tumor size had a significant impact on surgical margin status (p=0.047). Tumor size significantly affected survival time with larger tumors being correlated with shorter survival (p=0.03). The results of our study suggest that grade, tumor size and recurrence are significant factors for MCT prognostication.

This article summarizes data of determining a therapeutic dose of a new immunocorrecting drug, dermatocytoglobulin, developed by the authors of this study. It has been established that this drug has a therapeutic immunocorrecting effect on allergic dermatitis. Subjects of the study cattle, horses, of Raimbek – Commercial Diary Farm, 56 dogs and 35 cats admitted to a veterinary clinic. It has been established that administration of dermatocytoglobulin in trial groups reduces autoantibody titers until their elimination on the 12th day, dose of 0.2 cm3 per 10 kg while autoantibody titers in control groups were identified on the 30th day.