The objectives of the investigation were to determine effect of hull-less gene on grain quality traits (crude protein, starch and β-glucans) and to evaluate the heritability of these traits in the F4 and F5 generations of six crosses between covered and hull-less barley (Hordeum vulgare L.). The trial was carried out at the State Stende Cereal Breeding Institute during the years 2007 and 2008. Mean value, range and the coefficient of variation of traits for covered and hull-less lines within each cross combination were calculated. Heritability of quality traits was estimated as intergeneration correlation (r) and linear regression coefficients (b). The highest intergeneration correlation values were observed for starch content within covered and hull-less barley populations (rF4:F5=0.609 and 0.635 respectively). Moderate intergeneration relationship was observed for crude protein content within covered barley population (r=0.582), and for β-glucans content within hull-less barley population (r=0.593). The regression estimates of heritability were higher for starch content for both covered and hull-less barleys (b=0.549 and 0.703 respectively). Due to effects of genotype and environment interactions the regression estimates of heritability for crude protein and β-glucans content for both covered and hull-less barley were low (b varied from 0.347 to 0.373).

The main purpose of the research was to investigate the physically-chemical parameters of organic and conventional hull-less barley (Hordeum vulgare) harvested in Latvia. In the experiment the following hull-less barley harvested in 2012 from the State Priekuli Plant Breeding Institute (Latvia) was used: ‘Irbe’, PR4651, PR3808.21 and PR5099. The following quality parameters of grain – protein, starch, the bulk density and β-glucan content – were measured by standard methods – Infratec™ 1241 Grain Analyser (Denmark) corresponding to ISO 12099; thousand-grain weight (TGW) (ISO 520:2010); the falling number (ISO 3093:2009); moisture content (LVS 272:2000) were also determined. In the present experiments no significant differences in protein, moisture, starch, β-glucan content and TGW were detected, however, significant differences in the bulk density of all analyzed hull-less barley samples were established. The lower analyzed parameter value of 780 ± 1 g LE-1 was obtained for the conventional and organic hull-less barley PR 5099. However, the higher bulk density value of 821 ± 1 g LE-1 was obtained in the conventional hull-less barley variety ‘Irbe’. No significant differences in the bulk density of conventional and organic hull-less barley ‘Irbe’, PR4651 and PR3808.21 were found. Significant differences in the falling number were detected in the analyzed hull-less barley samples harvested in the conventional and organic farming. The lower falling number value was obtained in the organic hull-less barley line PR 5009 – 81 ± 4 s, the higher in the conventional and organic hull-less barley line PR 3808.21 as 362 ± 5 s and 373 ± 2 s, respectively.

In recent years, the effective immunostimulating properties of β-glucans have been widely proven not only in mammals, but also in aquatic animals. The aim of this study was to determine the effect of dietary β-glucan supplements on the percentage of individual types of leukocytes and the haematocrit of the sea trout (Salmo trutta L.). Investigations were performed at the state fish farm ‘Tome’, hatchery ‘Pelči’ of the Institute of Food Safety, Animal Health and Environment ‘BIOR’, during a six month period (September 2018 – February 2019). On the basis of feeding with immunostimulant diets, a total of 15 000 sea trout were assigned into five groups. For blood collection healthy fish were randomly selected once a month, five 5 individuals from each group. Blood smears were made to determine the leukocyte differential count. Sampling was done once a month (September 2018 – January 2019) (n=125). Haematocrit was determined by the standard microhaematocrit method. Sampling was done once a month (January 2019 – February 2019) (n=50). We concluded that the dietary β-glucan supplements can improve the haematocrit level and effect the percentage of individual types of leukocytes of the sea trout, stimulation of the preparation for parr-smolt transformation.

Grain samples of 51 spring barley (Hordeum vulgare L.) genotypes, including two-row, six rows, covered and hull-less were used for investigation of endosperm beta-glucan content. A significant difference (p-value is less than 0.001) between genotypes in beta-glucan content was observed. On average, the six-row barleys had slightly higher beta-glucan content. Far six-row genotypes it ranged from 35.9 to 47.1 mg kgE-1 with the mean value of 41.4 mg kgE-1, but for tw0-row barleys - 38.0 mg kgE-1 within the range of 33.2-44.9 g kgE-1. Six-row genotypes had a higher coefficient of variation in comparison with two-row genotypes. Hull-less genotypes had significantly higher beta-glucan content than the covered ones (49.3 mg kgE-1 and 38.9 mg kgE-1 respectively). The standard deviation of the mean values of beta-glucan content for covered genotypes was lower than for the hull-less ones. The mean values in 2005 were well plotted against data from the previous season for genotypes included in the two years trial. There were two distinct clusters of covered and hull-less genotypes. Beta-glucan content for varieties bred in Latvia ranged from 33.2 mg kgE-1 for two-row variety 'Malva' to 46.0 mg kgE-1 for six-row variety 'Druvis'.