Plant factories provide possibilities to get optimal yield of green leafy vegetables entire year independent from the season. The technology of light-emitting diode (LEDs) light has become one of the most powerful tools in photophysiological researches of various horticultural plants. In order to manage plant physiology and improve productivity, it is necessary to develop new LEDs technologies in horticulture. The aim of the study was to investigate different lighting intensities and elevated red light (660 nm) effect on tatsoi photosynthetic parameters. All plants were grown under the same light sources where overall photosynthetic photon flux density (PPFD) varied from 200 to 300 µmol mE-2 sE-1. Three days before harvesting red light intensity (640 and 660 nm) was increased until 132 µmol mE-2 sE-1 and 660 nm until 188 µmol mE-2 sE-1 at 16 hours photoperiod. In the last treatment merely 24 hours photoperiod was applied. The photosynthetic indices in tatsoi were determined by non-destructive methods using LI-6400XT portable photosynthesis system, OS5p fluorometer, DUALEX optical sensor and CID leaf spectrometer. Research was performed in 2017 – 2018 winter season. The obtained data revealed that the total photosynthetic photon flux density (PPFD) at level of 200 µmol mE-2 sE-1 was sufficient for optimal CO2 assimilation in tatsoi plants. The increased PPFD of LED light at pre-harvest stage resulted in reduced photosynthetic parameters of plants.

Light is one of the most significant environmental factors affecting the growth of plants, as it is the driving force of photosynthesis. Among others, the red and blue light are the most relevant, as these spectral regions are absorbed by chlorophyll the most. In addition, red and blue light trigger specific photomorphogenic responses that allow plants to capture the available light efficiently. Accordingly, the proportion of red and blue light (R:B ratio) is considered one of the most important characteristics of light for plants, as optimal R:B provides balanced growth and photosynthesis. The aim of this study was to evaluate how the in vitro cultures of hybrid aspen (Populus tremula L. × P. tremuloides Michx.) are affected by different R:B ratios under fixed illumination intensity. We examined the growth characteristics of plantlets under wide spectrum LED luminaires with three different R:B proportions – 1:1, 7:3 and 9:1. While photosynthesis-related variables were significantly affected by light, the effect on morphology was less pronounced. Overall, increased proportions of red light decreased the photosynthetic performance of plantlets without giving significant benefits in the form of longer shoots that could be used to facilitate propagation effectiveness. Nonetheless, the effect of light treatment remains at least partially clones-specific and should be considered in case of further application for propagation purposes.