The EU Directive on the promotion of the use of biofuels or other renewable fuels for transport (CO M 2003/30/EC) states that the proportion of biofuels in 2010 must make 5.75% of non-renewable fuels, but in 2020 - 10%. The only way for Latvia to fulfil the Directive 2003/30/EC is to use winter rye because it is one of the oldest cereals grown in Latvia, and Latvia has a long-lasting experience in growing it. A field trial of winter rye (Secale cereale L.) was arranged in the Agricultural Science Centre of Latgale from 2005 to 2007. The influence of four winter rye varieties and five fertilizer rates on grain yield and starch content was researched for three years. The year (factor A), the varieties (factor B), the fertilizer rates (factor C), and the interaction of factors AB, AC , BC, and ABC significantly influenced the yield of grain and the starch content with 95% probability. It is recommended to use good quality grain with high starch content and low protein content for bioethanol production. The varieties of winter rye 'Kaupo', 'Amilo', and 'Walet' are the most suitable for ethanol production in Latgale region.

Grain after enzymatic treatment, which is a starch-containing raw material, is used for ethanol production. Bioethanol production in Latvia began in 2006. Extraction of biofuels is a clean process, because the byproduct is used in various sectors of the economy. The bioethanol in Latvia was derived primarily from winter wheat, winter rye, and winter triticale. The objective of the research is to determine the different nitrogen fertiliser rates required for winter cereal crop yields and bioethanol outcome. The trials were carried out from 2005 to 2008 in Agricultural Science Centre of Latgale (Latvia). The method (ethanol outcome) is based on fermentation of saccharified cereal samples by yeast Saccharomyces cerevisiae followed by the calculation of ethanol outcome and speed of fermentation. The highest starch content was in winter wheat and winter triticale grain, but the lowest - in winter rye grains. A close negative correlation (p is less than 0.05) was found for winter triticale and winter wheat between the ethanol outcome and thousand grain weight. Production of bioethanol from rye starch content is used with full utilisation of grain. The winter wheat has the largest ethanol outcome from one hectare.

The present study was undertaken to estimate enzymatically hydrolysed and non–hydrolysed wheat (Triticum aestivum) and rye (Secale cereale) bran microflora. Enzymatic hydrolysis was accomplished by α – amylase from Bacillus amyloliquefaciens and by Viscozyme L which contain a wide range of enzymes responsible for the breakdown of carbohydrates into simple sugars. Wheat and rye bran samples were collected from native mills, namely Stock Company (SC) ‘Rigas dzirnavnieks’ wheat bran with large particle size (WLSR), SC ‘Jelgavas dzirnavas’ rye bran with small particle size (RSSJ), SC ‘Dobeles dzirnavnieks’ wheat bran with small particle size (WSSD) and wheat bran with large particle size (WLSD). Gained results indicate that before enzymatic hydrolysis all of the bran samples showed similar microbiological contamination with total plate count (TPC), yeasts and lactic acid bacteria. Enzymatic hydrolysis of bran gives the possibility to partially eliminate the microbiological contamination with TPC, yeasts and lactic acid bacteria. The amount of microorganisms after enzymatic hydrolysis (before storage) were decreased and ranged from 5.26 ± 0.04 to 5.45 ± 0.01 log CFU gE-1, from 4.81 ± 0.01 to 5.60 ± 0.05 log CFU gE-1, and from 4.09 ± 0.01 to 5.10 ± 0.05 log CFU gE-1, respectively. After eight weeks of storage (temperature – 20 ± 1 °C, relative humidity – 40 ± 1%) enumeration of microorganisms showed significant decrease of colony–forming units in all bran samples. The amount of TPC, yeasts and lactic acid bacteria in the control bran samples fluctuated in a range from 4.84 ± 0.04 to 5.49 ± 0.05 log CFU gE-1, from 4.86 ± 0.03 to 5.25 ± 0.03 log CFU g-1, 3.53 ± 0.03 to 4.21 ± 0.02 log CFU gE-1 respectively.

Malt is a natural food product produced by germinating cereal grains. Safety of cereal grains and cereal products is a very important area. Experiments were carried out at the Faculty of Food Technology of the Latvia University of Agriculture. The research object was rye malt. The purpose of the research was to investigate and compare the influence of copper and selenium on microbiological indicators of rye malt. Rye grains of 96% viability were soaked and germinated at temperature +6 ± 2 °C for 4 days, using copper(II) sulphate pentahydrate CuS04*5H2 O solutions (Cu concentration 10 mg LE-1, 50 mg LE-1, 100 mg LE-1) and for 3 days using sodium selenate Na2 SeO4 solutions (Se concentration 3 mg LE-1, 5 mg LE-1, 10 mg LE-1) then the soaked grains dried in the oven for 24 hours. The total plate count and yeast colony forming units were determined in rye malt samples. The obtained results showed that the increasing of copper and selenium concentration in solution significantly changes microflora of rye malt comparing with the control sample.

Renewable energy resources play an important role in energy production both in Latvia and in the world. Bio-ethanol is used as a substitute for oil products in various countries of the world. It is produced from the plants containing starch: cereals, potatoes, beet, maize. The task of the research was to evaluate the suitability of different varieties of winter wheat, triticale and rye for extraction of bio-ethanol in Latvia. The research was carried out at the State Stende Cereals Breeding Institute in 2009, and the following varieties and lines of cereals were examined: winter wheat varieties 'Mulan', 'Skalmeje', and the line '99-115', developed at the State Stende Cereals Breeding Institute; winter triticale varieties 'SW Valentino', 'Dinaro', and the line '0002-26', developed at the State Priekuli Plant Breeding Institute; winter rye varieties 'Matador', 'Placido' Fp and 'Dankowskie Nowe'. The content of crude protein and starch of grains, the grain yield, and the bio-ethanol yield were determined. The highest bio-ethanol yield was acquired from the winter wheat and triticale varieties that had the highest starch content and the lowest crude protein content of grains. The best results were obtained from winter wheat line '99-115' and variety 'Mulan' (409.4 and 406.8 L tE-1), triticale variety 'Dinaro' (423.3 L tE-1), and winter rye variety 'Matador' (370.1 L tE-1.).

Brown rust caused by Puccinia recondita is a significant disease in cereal growing areas worldwide. On average, brown rust can cause yield losses from 10% to 40% resulting in economic losses. The disease damages the leaves, stems and glumes of cereals. Classification of the pathogen causing brown rust has undergone several revisions. There are two different opinions about the causal agent causing brown rust on rye and wheat. Some scientists consider that the causal agent of brown rust in wheat (Triticum aestivum L.) and rye (Secale cereale) is included in the broad species of P. recondita defined by Cummins, but other scientists consider that there are two species causing brown rust – P. recondita in rye and P. triticina in wheat. There are many studies about the races of P. recondita in wheat: in North America, 70 races are collected every year; in Canada, 35 races have been found; in Europe, 105 races have been found. Unfortunately, there are no studies about the races of the pathogen of rye. P. recondita is a heteroecious fungus with a complicated life cycle. For successful development, the fungus requires cereals as primary hosts and different alternative hosts, depending on the specialization of the pathogen. Specific studies about the biology, distribution and harmfulness of Puccinia recondita in Latvia are necessary. Monographic method was used for this study. The aim of this article is to summarize the information from the literature about Puccinia recondita, the causal agent of brown rust, with emphasis on the occurrence, harmfulness, taxonomy, and life cycle of P. recondita f. sp. secalis.

Germinated grains are added to wheat dough with the purpose to promote the biological value of bread. As a result, a new product was obtained with a higher content of protein, fibre, B group vitamins; and vitamins C and E. The task was to investigate gluten quality changes at grain germination time, wheat dough rheological properties changes with various wheat, rye and barley amount additions, germinated for a different time. With the purpose to save maximum stability value of gluten, it was ascertained that the germination time of wheat grain could not be more than 24 hours. The best dough quality was obtained with germinated wheat grain additive. Germinated rye and barley grain additive (more than the experimentally ascertained amount) increases dough softening, decreases dough development time and dough stability. Only adding experimentally determined optimal amount of germinated grain, which promotes high quality bread, could produce dough with accepted rheological properties.

The content of reducing sugars can be 30% higher if modified amylase is added to scald. Investigations were carried out with the rye-flour and fine flour scald. Photometrical method was used as a standard method for the determination of reducing sugars. Enzymes were inactvated using the advanced method. Results show, that the amount of reducing sugars is 1.8% lower in rye-flour and 2.1% lower in fine flour compare to the results obtained using the standard method.

The spread of thrips was observed in winter rye, wheat and triticale crops during the growing season of 2001 at the Lithuanian Institute of Agriculture in Dotnuva. Thrips started to spread in winter cereals of the end of tilling - beginning of stem elongation stage (DC 29-30). In winter rye and triticale the spread of thrips was similar. There were two well-defined peaks of thrips occurrence. The highest incidence of thrips was on winter rye. There were 100% infested stems and 15.4 thrips per infested stem in rye, 90% infested stems and 6.9 thrips per infested stem in triticale and 84% infested stems and 2.9 thrips per infested stem in wheat.