In this study wool characteristics of Libyan sheep in north-eastern Libya were investigated in a coastal region extended from El-Marj (32 25 N and 20 30 E) to Emsaad at the north-eastern border of the country (31 33N, 25 6 E). Twelve locations, one flock per flock were studied. Three sites have been sampled from each animal (Shoulder, mid-side and breech). Mean staple length and fiber diameter were 12.16cm and 38.43 µm respectively. In this study, staple length and fiber diameter didn’t significantly vary between locations studied. Sample site on the body of the animal significantly affect both staple length and fiber diameter, with breech position samples showed the shortest staple length and the thickest fiber diameter (P< 0.05). There was no difference between samples taken from mid-side or shoulder in all measured traits. Results of this study showed that Barbary sheep raised in the eastern part of the country characterized by a long staple and a large fiber diameter, and then confirmed the previous studies studies stated that this breed could be classed as a long carpet wool breed. Samples taken from mid-side position could be representative of Barbary sheep fleece