In this study, a conceptual rainfall-runoff METQ model, version METQ2007BDOPT, to simulate daily runoff was applied. The model structure and parameters were fundamentally the same as in the METQ98 model with some additional improvement and semi-automatical calibration performance. The model has proved to be successful for both small (the Vienziemite Brook, 5.92 km2 ) and large (the Daugava River, 81 000 km2 ) drainage basins. The model METQ2007BDOPT was calibrated to the six different size river basins (the Pērse, the Malta, the Neriņa, the Imula, the Malmuta and the Iecava). These pilot river basins are characterised by one or two prevailing natural conditions such as hilly agricultural lands, agricultural lowlands, sandy lowlands, forested areas, swamps or lakes. The results of calibration showed good coincidence between the measured and simulated daily discharges. The Nash – Sutcliffe model efficiency coefficient NSE varies from 0.52 to 0.78 and Pearson correlation coefficient r from 0.65 to 0.88 for the six river basins with calibration and validation period from 1956 to 2015. In this study, we found some relationships between the model parameter values and physiographic sub-catchment characteristics.

Lake sediments have a broad range of elementary and organic substance content. Bottom sediments collect decaying organic debris of aquatic plants and animals mixed by water drift with mineral constituents - deposited in the bottom of the lake in anoxic conditions. Lake Alūksne is situated in Northeast Latvia and is of glacial origin. Its water and bottom sediments chemical content depends on formation conditions – influenced also by anthropogenic activities. In order to determine environmental quality for restoration purposes and sediment recovery, geochemical research is needed. This paper aims to study bottom sediments – texture, ash part, organic compounds and metals to pinpoint necessary steps that are crucial for distinguishing environmental quality of the lake through geochemistry research. Bottom sediments contain sapropel with large admixture of organic compounds and microelements that may significantly influence biota and human health if concentration exceeds natural background levels. When bottom sediments are recovered, it may improve oxygene conditions in the lake, extracted sapropel can be used as a fertilizer, therapeutic agents, supplements for farm foods. Generally, sapropel is improving the structure of agricultural soils, increasing the cation exchange capacity and serving as binding material for complexes formation; thus diminishing ecotoxicological exposure threats of heavy metals, increasing yields and albumen and protein quantity in plants cultivation products. As these sediments can be used for soil fertilizing and crop production improvement in agriculture, mechanisms of ecotoxicological impact to various soil types and agricultural plant ecology should be researched.

Eutrophication caused by water pollution with nutrients (nitrogen (N) and phosphorus (P)) is one of the main environmental problems nowadays. Poor water quality might be caused by many natural and anthropogenic factors. The most common anthropogenic factors are water pollution caused by intensive agriculture (e. g. intensive fertilizer use, high density of livestock) and discharges from municipal waste water treatment plants (WWTP). In this study, nutrient load from WWTP to the River Berze basin in time period from the year 2005 to the year 2015 was described. In total, 23 WWTPs of the study area were analysed using descriptive statistics to calculate average values of nutrient amount and concentrations discharged to River Berze basin. From 2005 to 2015 average N and P load from WWTP to the River Berze basin have been reduced, but concentration of N and P in treated wastewater (WW) have increased. The largest WWTP of the study area – Krigeri (WWTP of city Dobele) is subject to the regulations of treated WW quality set by Republic of Latvia Cabinet Regulation No. 34. The WWTP Krigeri meets the regulations, but overall situation in the study area suggests that strict control of smaller WWTP should be made.

Our previous research was focussed on evaluating spaces in the Soviet period housing estates in Riga according to theoretical model of spatial measurements. This model was based on assumption established ex situ that liking and perception of urban space is closely related to buildings. Namely, to the way walls of the buildings shape a setting in terms of its height, width, length. This current paper sets the goal to analyse the impact of landscape elements such as trees, benches, elevations of earth surface, playgrounds on the perception and preference of open spaces in situ. This is done to verify the hypothesis that landscape elements as well as the method of research play a role in our knowledge on how urban spaces are regarded. Semi-structured interviews in walk-along modus are used to talk to inhabitants about the ways they see and like yards and streets in three residential areas: ‘Agenskalna priedes’, ‘Kengarags’, ‘Zolitude’. The interview materials are analysed discursively. The extracted information is compared to the data previously acquired by using the model of spatial measurements. The results of interviews show that trees are the landscape elements that have the largest impact on how residents perceive the size of a space and how they like a setting. There are also other elements such as benches, playgrounds, elevations that play a role. The interview material displays deviations in preference and perception of the size of space compared to the model of spatial measurements. Yet these deviations can be adapted to the model.

Raw milk cheeses are known to have more intense and strong flavour and different texture due to natural microbiota and enzymes. Nevertheless, there are concerns about safety of these products. For microbial inactivation heat treatment of milk is used, but it can adversely affect the flavour, taste and texture of the product. Therefore, applying non-thermal technology such as high pressure processing is attracting alternative. The aim of this study was to examine the effects of high pressure treatment of cow`s milk at a wide range of pressures (400–600 MPa) on milk rennet coagulation time, curd firmness and curd yield. Processed milk samples were subjected to enzymatic coagulation using commercial rennet to determine rennet coagulation time, yield of coagulum and curd firmness. High pressure processing insignificantly influences coagulation properties of whole milk. However, the magnitude of changes depended on applied pressure. Rennet coagulation time and curd yield were significantly different (p is less than 0.05) among the pressure treated milk samples. The higher firmness of the curd form pressurized milk than that of raw or pasteurized milk, evaluated positively. The main effects of high pressure treatment in milk appeared to involve dissociation of casein micelles from the colloidal to the soluble phase. This study suggests that high pressure treatments of milk at 500 MPa or 550 MPa for 15 min may be beneficial for improving the coagulation properties of milk. These positive effects indicated that high pressure processing may have potential for new cheese varieties development.

Fresh meat is a highly perishable product due to its biological composition as it serves as an ideal environment for the growth and propagation of microorganisms and common food-borne pathogens. High pressure processing (HPP) is a cold pasteurization treatment to extend shelf-life while preserving the sensory and nutritional characteristics of the product. The aim of this research was to evaluate the effects of HPP on the fresh porcine Musculus longissimus lumborum microbial load and related physical properties (pH, water activity aw, and moisture content). Vacuum packed meat samples were treated at 50, 100, 200, 300, 400, and 500 MPa for 1, 5, and 15 min in a high-pressure processor ISO-Lab S-FL-100-250-09-W (Stansted Fluid Power Ltd., UK). Pressure treatment above 300 MPa resulted in a significant (p is less than 0.05) decrease of total plate count. However, the studied pressurizing time had no significant effect on microbial lethality at the same pressure applied. Other important parameters such as water activity, moisture, and pH were determined as they directly affect microorganism growth and resistance to pressure. A slight increase in pork pH was observed with increased pressure. No significant changes in water activity and moisture content were observed as a result of high pressure treatment. For future researches it would be important to evaluate the dynamics of microbial growth during storing as part of cells after pressure treatment are injured and not eliminated immediately; therefore, microbial count may further decrease during cold storage.

High economic losses in agriculture can be caused by pig disease like Postweaning Multisystemic Wasting Syndrome (PMWS). Porcine circovirus-2 (PCV2) is the primary agent of PMWS. It is known that PMWS causes cellular and humoral immunity disorders, therefore it is considered a general immune deficiency disease in piglets. It is still unknown, why piglets with PMWS have severe loss of lymphocytes in lymphoid tissues and in the blood. Thus, the hypothesis of the study was that lymphocyte depletion occurs because of lymphocyte life-cycle delay in pigs infected by PCV2. The activity of cell life-cycle is attributed to protein Ki67. This protein is found in all active phases of the cell like interphase G1, S, G2 and mitoses. However, protein Ki67 is not observed at all in the cell’s “quiet” phase G0. The aim of our work was to investigate the lymph node cell proliferation activity of pigs infected by PCV2. In total, 42 pig carcasses were collected from 5 to 15 weeks old pigs with PMWS clinical manifestation. Lymph nodes were selected from each pig carcass for histological and immunohistochemical testing. We concluded that B lymphocyte population was reduced first in the lymph node with PCV2, but the number of T lymphocytes decreased later. PCV2 decreased B lymphocyte proliferation activity.

The aim of this study was to investigate the patterns of antibiotic resistance of Aeromonas spp. bacteria isolated from the sea trout (Salmo trutta) from the state fish hatcheries of the Institute of Food Safety, Animal Health and Environment ‘BIOR’, Latvia. Bacteriological investigations were performed at four state fish hatcheries located in the drainage basins of the main Latvian rivers – Daugava, Venta and Gauja, during the five-year period (2012 – 2016). In fish with visible clinical signs, bacteriological samples were collected from heart, liver, spleen, kidney and ulcer surfaces. Aeromonas hydrophila and Aeromonas salmonicida were isolated from sea trouts. A total of 52 individual sea trouts were examined. Resistance to amoxicillin, ampicillin, cephalexin, colistin, doxycycline, enrofloxacin, erythromycin, florfenicol, gentamycin, kanamycin, lincomycin, neomycin, oxytetracycline, spectinomycin, streptomycin, tetracycline, trimethoprim/sulfamethoxazole was tested. The results of this study suggest a multi-drug resistance pattern among the A. hydrophila isolates. All the isolates were resistant to amoxicillin (100%), ampicillin (100%), cephalexin (100%) and erythromycin (100%). The lowest level of resistance was found against florfenicol (4.55%), gentamycin (4.55%), kanamycin (4.55%), but susceptibility was recorded to enrofloxacin, neomycin and trimethoprim/sulfamethoxazole. A. salmonicida isolates were resistant to oxytetracycline (9.38%) and tetracycline (9.38%). For other antibiotics A. salmonicida isolates were susceptible.

Renewable energy resources have been developing very fast due to negative effects and finite reserves of the fossil fuels. Biomass is ranked among the most promising renewable energy resources within the Central Europe. Corn (Zea mays L.) is currently the most widely grown crop in the Czech Republic; nevertheless, the cultivation of corn provokes soil erosion by water. Perennial energy grass called tall wheatgrass (Elymus elongatus subsp. Ponticus cv. Szarvasi-1) is supposed to be a good and environment-friendly alternative to corn. Field trials including these two crops were established in the experimental locality of South Bohemia. Their yield potential was monitored during spring harvest periods (use for combustion). Dry phytomass was fundamentally analysed (N, C, H, S) and higher heating value was determined too. Universal Soil Loss Equation was calculated for both crop species. Corn provided much higher average yield in a three-year interval; corn phytomass reached higher heating value as well. The area of Elymus elongatus should enlarge considerably, if we wanted to get the identical amount of energy from corn and Elymus elongatus. However, we found that, compared to Zea mays L., water erosion theoretical land losses would be several times less serious for Elymus elongatus.

This work deals with the baking quality of the spelt wheat grain (Triticum spelta L.) compared with bread wheat (Triticum aestivum L.). Mixed flours were made of different share of spelt wheat and bread wheat (in total 11 mixtures) in 2016 in the Česke Budejovice in the laboratories of the Faculty of Agriculture. The technological quality of these mixtures was analysed, focusing on standard evaluation methods (protein content, characteristic of gluten or swellability of protein). The analysis was supplemented by complete rheological analysis made by Mixolab II. Bread was used as a model product. Subsequently, sensory evaluation of baked bread from the previously prepared mixtures was done. Part of the analysis was to estimate the economic basic bread recipe with different proportions of bread wheat and spelt wheat. The results were statistically analysed via STATISTICA 9.1 (StatSoft, Inc., USA). It was proved that the flour made of spelt can give cereal products with a higher nutritional value. The results have shown that the spelt grain is much more suitable for baking. Its advantage is the higher protein content and higher resistance of kneading of the dough and starch gelatinization rate, which was statistically confirmed. The main disadvantage is the higher price of spelt. According to the results, the ideal utilization of spelt wheat based on sensory analysis and economic calculations seems to be the mixture of spelt wheat and bread wheat, which results in an undeniable decrease of the product cost, and hence effects the common customer choice and taste preferences.