The study consists of literature review on potato (Solanum tuberosum L.) initial seed material – minitubers production. This paper covers aspects of healthy potato microplants production techniques and subsequent greenhouse minitubers production methods. The diversity of conventional minituber growing techniques (on solid medium in greenhouses) is discussed. Review showed that obtained minitubers number depends on growing methods and variety. Physical manipulation during in vitro phase could have positive effects on minitubers yielding capacities.

Field trials were carried out at Jõgeva Plant Breeding Institute in 2008 – 2011 to identify the possibilities of using early red clover (Trifolium pratense L.) ‘Jõgeva 433’ (diploid), Washington lupine (Lupinus polyphyllus Lind.) ‘Lupi’ and crimson clover (Trifolium incarnatum L.) as green manure. Fresh material of the above species was ploughed into the soil in autumn of the sowing year. Fertilizer value was quantified through yield and grain quality of spring wheat ‘Vinjett’ and barley ‘Inari’. The composition and amount of ploughed biomass were recorded. By the time of ploughing, Washington lupine had produced the most abundant biomass. >From studied species crimson clover had the lowest fertilizer value – only by 6–7% extra yield of spring wheat in the following year. Crimson clover had no residual effect of fertilization in the second year. The fertilizer values of red clover and Washington lupine were approximately equal. Their effect on yield increase of spring wheat and barley lasted for three years, on grain quality for two years. Red clover, ploughed into the soil in the year of sowing, resulted in maximum spring wheat yield increase of 23.9%, compared with N 0 treatments; that of Washington lupine was 21.3%. The residual positive effect in the second year quantified as barley production increased by 6.2% in Washington lupine and 7.9% in red clover. The yield increase in the third year was 3.9% and 12.1%, respectively. Green manure increased the contents of crude protein and gluten in spring wheat and crude protein content in barley.

One of most important traits in dairy farming is cow (Bos primigenius taurus) longevity. In last few years the length of productive life in Latvian dairy cow population significantly decreased. Cow longevity depends on a large amount of genetic and non-genetic factors. Data from 1037 excluded Latvian brown (LB) breed cows were included in the analysis. Cows were born in the period from the year 2002 to 2006 and a farm size was 9 – 163 cows per farm. Farms were dislocated in all main regions of Latvia. Average life length of cows excluded from the herd was 2463.0 days in small farms, 2234.6 days in medium size farms and 2089.5 days in large farms. Cows’ productivity in one life day in small farms was 5.9 kg energy corrected milk (ECM), in medium size farms 6.2 kg ECM and in large farms 6.5 kg ECM. Large farm cows had longer life length in days (p is less than 0.05), but higher productivity in one productive day (p is less than 0.05) and productivity in one life day.

Kvass is a non-alcoholic beverage produced by fermenting kvass mash with yeast; alcohol content in kvass must be less than 1.2% alcohol by volume. Microbiological safety of kvass is an important issue because European Regulation No 2073/2005 on microbiological criteria for foodstuffs does not provide microbiological criteria for kvass production. Microbiological safety of kvass depends on raw materials, personal hygiene, environment, kvass blending and filtration. Experiments were carried out at the Latvia University of Agriculture Department of Food Technology from November 2013 to January 2014. The aim of this work was to assess the microbiological environment changes during kvass production process and shelf-life. Understanding the development of dynamic of microbiological environment provides a better management for kvass production processes. Samples of bread kvass were analysed during production (12 and 13 h) and storage (36, 60, 84, 132, 136 h) at 3 ± 1 °C to determine kvass quality. Yeasts (LVS EN ISO 21527 - 2: 2008), lactic acid bacteria (ISO 9332:2003) and total plate count (LVS EN ISO 4833:2003) were determined in kvass samples. Microorganisms in kvass were identified using API identification system; the dominating microflora in kvass was Saccharomyces cerevisiae and Leuconostoc mesentericus. Changes of total plate count during fermentation and maturation were not significant (p is greater than 0.05).

The objective of the study was to investigate the microbiological content of cow (Bos primigenius) milk in Latvian organic farms according to season and herd size with a purpose to detect their impact on the distribution of mastitis causing pathogens in milk. Samples were collected in 14 organic dairy farms of Latvia, 4 times through 2012: in winter, autumn, spring and summer period. Raw milk samples (n=564) obtained from cow composite milk were studied. The samples were divided into three groups in accordance with the number of cows in the cow-shed: A (3-30), B (31-60) and C (61-124). The total colony count (TTC) and the isolation of mastitis causing bacteria were analysed using standard methods. Bacterial growth occurred in 90.4% of samples. Isolated microorganisms belonged to 35 species, and the following bacteria were the most prevalent agent, including Coagulase negative staphylococci in 29.4%, Staphylococcus aureus in 24.1%, Kocuria kristinae in 12.9%, and Enterobacteriaceae spp. in 10.3% out of 564 milk samples. Depending on the season, the average number of TCC was the lowest in summer (4.66 ± 4.01 log10 CFU mLE-1), moderately higher in spring and winter (4.72 ± 4.18 and 4.82 ± 3.54 log10 CFU mLE-1, respectively), but significantly higher in autumn (5.43 ± 4.80 log10 CFU mL-E1). Coliforms were not isolated from group A herds instead of B (3.1%) and C (4.6%). Occurrence of Staphylococcus aureus was noticeably higher in B (19.1%) and C (20.8%) than in A (12.6%) herds. Most of group A herds met the milk quality requirements, while group C herds produced more contaminated milk.

In goats (Capra) the growth and functional development of certain parts of the multi-chambered stomach continue during the first few months after birth. The aim of this research was to clarify morphofunctional changes in the kids abomasa and rumina and live weight gain during the first 8 weeks of life. Research was performed in two parts. In the first part (P1) we used Saanen breed kids which were all kept in the same conditions and in the second part (P2) we used Saanen breed kids which were separated in two groups with different feeding diets. In P1 the stomach contents of the goat kids were collected after slaughter (on day 1, 17, 25 and 30), weighed full and empty. The gastrohromoscopical method proved that in the newborn kids the area where the abomasal pH is 3.0 and lower is about 10% of the surface of the abomasum, but in age of three weeks it is composing 80% of the mucosal surface of the abomasum. In P2 were two groups – in the first group (MMG) kids which were fed with dairy (mother) milk and lived with mothers, the second group (MRG) kids were fed with milk replacer and lived separate from mothers. The stomach contents were collected after slaughter(at day 45 and 60).We confirm that the most important age of stomach development and kids growth in postnatal period are the first 45 days. During this period the most significant differences can be observed. On day 60 there are no significant differences (p is greater than 0.05) between MMG and MRG stomach development.

Listeria monocytogenes is a pathogen that causes infectious diseases in animals and foodborne infection in humans. The aim of this retrospective study was to analyse L. monocytogenes caused abortions in cattle in Latvia in 2013, and to describe the potential reasons of these abortions. In total, 186 cattle abortion cases were investigated. The pathological material from aborted foetuses included samples of brain, heart, liver, spleen, kidneys and lung, liquid of stomach and liquid from thoracic and abdominal cavities. These samples were tested with bacteriological methods according to ISO 11290-1:2007. L. monocytogenes was found in 44 (23.7%) cases of cattle abortions. Positive cases were more distributed in the central and the south-eastern parts of Latvia, because in this territory winters tend to be wetter and colder than in other parts of Latvia. Seasonality was observed of L. monocytogenes caused abortions and the majority of cases occurred during spring and autumn, when the cattle were fed with silage. All abortion cases were observed in the second and the third trimester of the gestation. L. monocytogenes caused abortions occurred in cattle of different ages, but significantly (p is less than 0.05) more often – in 3 years old cattle.

Nowadays excessively used corticosteroids in veterinary medicine induce steroid hepatopathy in dogs (Canis lupus familiaris). The objective of this study was to determine the possibility of the hepatoprotectants to decrease the corticosteroid-induced alteration in such dogs’ blood serum enzymes as alaninaminotransferase (ALAT), gammaglutamyltranferase (GGT), alkaline phosphatase (AP) and corticosteroid-induced thermostable alkaline phosphatase (cAP). The study took place in private veterinary clinics in Riga, Latvia, during 2013, with the permission of dogs’ owners. Twenty eight animals, which received corticosteroids due to present diagnosis, were divided into two groups. In the first group long-lasting corticosteroid methylprednisolone acetate injection was used once, while in the second group the hepatoprotectants were used after the injection of corticosteroids. It was discovered that after 14 and after 30 days of hepatoprotectants use, blood enzymes were significantly lower (p is less than 0.05) than in dogs that did not receive hepatoprotectants. In both groups the enzyme values did not reach the reference limits. The study is set to investigate further if and when the values reach the reference limits.

An important factor in the development of forest ecosystem is the ability to regenerate. Natural intensity of self thinning of a forest depends on the tree species and environmental conditions. Due to abiotic and biotic factors in a continuous forest tract, there appears a clearing, which, depending on the size, forms new growth conditions. Over time, the resulting new space is occupied by herbaceous and woody vegetation. Most often regeneration of a new forest depends on the size of the plot. The study was conducted in 2013 during the growing season in a typical broadleaf forest stand. During the study woody vegetation and projection coverage of herbaceous vegetation was registered in large clearings. Light conditions in the plots and under tree canopies, as well as soil parameters were ascertained. Based on the collected data, the view of the structure of woody vegetation, projection coverage of herbaceous vegetation, light conditions, temperature, soil moisture content and pH changes were obtained. In order to clarify the influence of microclimatic conditions on natural forest regeneration, the data on light and soil characteristics were analyzed. The aim of the study - was to determine the changes of woody and herbaceous vegetation in spruce stand clearings and to assess the impact of microclimate. During the study it was found out that in large plots dominated species demanding higher amount of light, while herbaceous vegetation was attributed to the third, fourth groups of aggressiveness. Naturally regenerated seedlings condition was mostly influenced by light conditions and soil moisture content.

The influence of forest distribution in the landscape on precipitation in middle latitudes is poorly researched. Moreover, results of such studies are controversial. Nevertheless, most researchers agree with findings, that influence of landscape roughness formed by forests on the amount of precipitation is significant. This study aimed to extend knowledge of the interface between forest distribution and the amount of precipitation during particular periods. Database of the amount of precipitation from 31 Lithuanian meteorological stations during the period of 2002-2010 was used in this study. Findings defined statistically significant correlations in the amount of precipitation increase due to shorter Euclidean distance to the nearest forest edge in the particular landscape (r = -0.421 – -0.359), p is less than 0.05), also shorter distance of the meteorological stations to the nearest forests (r = -0.380, p is less than 0.05) and higher density of the forests, treated as a perimeter per area ratio (PAR) (r = 0.359 – 0.551, p is less than 0.05).