As European Union member states – Latvia and Lithuania have changed their national height systems from Baltic Normal Height System 1977 to European Vertical Reference System realizations. Performing practical Global Navigation Satellite System (GNSS) measurements and obtained data mathematical processing, there is a possibility to calculate point height difference between GNSS data and data of 1st order levelling networks. The aim of the research is to gain insight for methodology development of geodetic point height testing using GNSS measurements. To achieve the goal following tasks are set: 1) perform GNSS measurements of first order levelling network in Latvia and Lithuania; 2) do data processing to get point ellipsoidal height and compare with the point normal height; 3) detect technically feasible accuracy of geoid model for precise height measurements with GNSS method within research area. Performing GNSS measurements at the same time in the territory of Latvia and Lithuania, the experience of mutual cross-border cooperation was accumulated. The results were adjusted with academic software package Bernese and were obtained point ellipsoidal and normal heights. The height measurement accuracy using GNSS method depends on geoid model accuracy and more precise results can be obtained increasing used geoid model accuracy to 1.5 to 2 cm.

The aim of the paper is to explore the possibilities of detection and reduction of land degradation in Smarde municipality rural territory (56°57′18″ N; 23°20′17″ E). To carry out land degradation prevention measures, initially the territories of degraded land should be determined. This paper highlights the field inspection method. For territory inspection a model was used that gave the opportunity to identify degradation types with their characteristic features and possibilities to reduce the land degradation. The territory of Smarde municipality rural territory was inspected in nature and degraded territories identified. The costs of land degradation elimination depend on the type of land degradation. In territories where the land degradation reduction or elimination has been done, its control has to continue in order to stop the development of land degradation.

Human milk provides infant with the required nutrients for growth and development. The aim of preliminary study was to determine macro- and micronutrients content in mature human milk among lactating women residing in Latvia and dietary habits affecting it. The study was carried out from November 2016 to February 2017. In total, 24 pooled diurnal milk samples were collected from mothers whose infants had reached the age of at least two months. Personal information of each participant was recorded, including mother’s age, weight and height parameters, sex and age of an infant, parity, breastfeeding pattern, milk expression method used for sampling. Eating habits were obtained using Monthly Food Frequency Questionnaire. Analysed elements were determined using inductively coupled plasma mass spectrometry (ICP‑MS Agilent 7700x, Japan). Concentrations ranged from 227.52 to 398.34 mg LE-1 for calcium, 58.56 – 256.38 mg LE-1 for sodium, 445.33 – 736.71 mg LE-1 for potassium, 25.73 – 49.52 mg LE-1 for magnesium, till 3.17 mg LE-1 for iron, 0.28 – 2.77 mg LE-1 for zinc, 0.06 – 0.43 mg LE-1 for copper, 2.00 – 44.00 µg LE-1 for manganese, 1.00 –10.00 µg LE-1 for chrome which is comparable to data obtained from other studies although chrome and manganese concentration was even higher than observed in literature. Cobalt and selenium content was below detection limit. Zinc content in human milk negatively correlated with baby’s age (p is less than 0.05). The content of majority of essential minerals in human milk was affected by mother’s dietary habits; however, more samples need to be analysed for representative conclusions.

Fresh meat is a highly perishable product due to its biological composition as it serves as an ideal environment for the growth and propagation of microorganisms and common food-borne pathogens. High pressure processing (HPP) is a cold pasteurization treatment to extend shelf-life while preserving the sensory and nutritional characteristics of the product. The aim of this research was to evaluate the effects of HPP on the fresh porcine Musculus longissimus lumborum microbial load and related physical properties (pH, water activity aw, and moisture content). Vacuum packed meat samples were treated at 50, 100, 200, 300, 400, and 500 MPa for 1, 5, and 15 min in a high-pressure processor ISO-Lab S-FL-100-250-09-W (Stansted Fluid Power Ltd., UK). Pressure treatment above 300 MPa resulted in a significant (p is less than 0.05) decrease of total plate count. However, the studied pressurizing time had no significant effect on microbial lethality at the same pressure applied. Other important parameters such as water activity, moisture, and pH were determined as they directly affect microorganism growth and resistance to pressure. A slight increase in pork pH was observed with increased pressure. No significant changes in water activity and moisture content were observed as a result of high pressure treatment. For future researches it would be important to evaluate the dynamics of microbial growth during storing as part of cells after pressure treatment are injured and not eliminated immediately; therefore, microbial count may further decrease during cold storage.

High economic losses in agriculture can be caused by pig disease like Postweaning Multisystemic Wasting Syndrome (PMWS). Porcine circovirus-2 (PCV2) is the primary agent of PMWS. It is known that PMWS causes cellular and humoral immunity disorders, therefore it is considered a general immune deficiency disease in piglets. It is still unknown, why piglets with PMWS have severe loss of lymphocytes in lymphoid tissues and in the blood. Thus, the hypothesis of the study was that lymphocyte depletion occurs because of lymphocyte life-cycle delay in pigs infected by PCV2. The activity of cell life-cycle is attributed to protein Ki67. This protein is found in all active phases of the cell like interphase G1, S, G2 and mitoses. However, protein Ki67 is not observed at all in the cell’s “quiet” phase G0. The aim of our work was to investigate the lymph node cell proliferation activity of pigs infected by PCV2. In total, 42 pig carcasses were collected from 5 to 15 weeks old pigs with PMWS clinical manifestation. Lymph nodes were selected from each pig carcass for histological and immunohistochemical testing. We concluded that B lymphocyte population was reduced first in the lymph node with PCV2, but the number of T lymphocytes decreased later. PCV2 decreased B lymphocyte proliferation activity.

The aim of this study was to investigate the patterns of antibiotic resistance of Aeromonas spp. bacteria isolated from the sea trout (Salmo trutta) from the state fish hatcheries of the Institute of Food Safety, Animal Health and Environment ‘BIOR’, Latvia. Bacteriological investigations were performed at four state fish hatcheries located in the drainage basins of the main Latvian rivers – Daugava, Venta and Gauja, during the five-year period (2012 – 2016). In fish with visible clinical signs, bacteriological samples were collected from heart, liver, spleen, kidney and ulcer surfaces. Aeromonas hydrophila and Aeromonas salmonicida were isolated from sea trouts. A total of 52 individual sea trouts were examined. Resistance to amoxicillin, ampicillin, cephalexin, colistin, doxycycline, enrofloxacin, erythromycin, florfenicol, gentamycin, kanamycin, lincomycin, neomycin, oxytetracycline, spectinomycin, streptomycin, tetracycline, trimethoprim/sulfamethoxazole was tested. The results of this study suggest a multi-drug resistance pattern among the A. hydrophila isolates. All the isolates were resistant to amoxicillin (100%), ampicillin (100%), cephalexin (100%) and erythromycin (100%). The lowest level of resistance was found against florfenicol (4.55%), gentamycin (4.55%), kanamycin (4.55%), but susceptibility was recorded to enrofloxacin, neomycin and trimethoprim/sulfamethoxazole. A. salmonicida isolates were resistant to oxytetracycline (9.38%) and tetracycline (9.38%). For other antibiotics A. salmonicida isolates were susceptible.

Organic farmers need varieties adapted to variable growing conditions, because in organic farms environmental conditions significantly vary in soil nutrient status, weed and disease pressure not only between years, but also among farms within a season. Modern varieties developed under high input agricultural systems do not always perform well under organic growing conditions. Therefore, farmers need such varieties of cereals that can adapt to variable growing conditions and maintain productivity. One of the ways to promote adaptation to environment is growing of variety mixtures. Field trials were conducted during 2014 – 2016 under organic and conventional farming systems in two locations with an aim to estimate the advantages of barley mixtures in respect of yield and its stability. The trial consisted of eight mixtures of barley varieties and breeding lines, components of mixtures in pure stand and three check varieties. The mixtures were combined by using two, three and five components. Three mixtures, each in one out of 11 environments significantly out–yielded, but one mixture had significantly lower yield than the average value of their components. Some mixtures showed a tendency to out–yield the average of components in both farming systems. All mixtures insignificantly out–yielded check varieties in 2015, but in 2016, yield was within the range between varieties with lowest and highest yield. The results of yield stability analysis showed that mixtures could be used as one of the approaches to stabilize yield – only one of eight mixtures had a lower yield than the average over all environments.

Environmental conditions and climate change on a global scale affect the overall agriculture and food supply. Edamame, also called vegetable soybean is an import vegetable in the world, especially in the East Asia. China is the world’s centre for vegetable soybean production. Edamame beans are harvested before full maturity when bean pods are green and just before turning to yellow colour. Most cultivated varieties in the world are of Japanese origin. The aim of this literature survey is to give a review on history and consumption facts of edamame, as well as on the yield and quality parameters of this fresh commodity. Monographic method has been used for this review. Edamame has a high nutritional and medical value. Composition of biologically active compounds in edamame plants is dependent on climate conditions, seasonal changes, cultivar properties and maturity stage. Vegetable soybean has a long cultivation history in the world. In Europe edamame is grown, but in Latvia edamame has not been grown until now, but there is some experience in soya grain growing. Pod colour is the main external quality factor of edamame. It can be influenced by light, moisture, fertilization, variety properties, planting density and harvesting procedures. Vegetation period for edamame in most cases is reported to be between 75 and 100 days. Environmental conditions and climate change on a global scale affect the overall agriculture and food supply. Edamame, also called vegetable soybean is an import vegetable in the world, especially in the East Asia. China is the world’s centre for vegetable soybean production. Edamame beans are harvested before full maturity when bean pods are green and just before turning to yellow colour. Most cultivated varieties in the world are of Japanese origin. The aim of this literature survey is to give a review on history and consumption facts of edamame, as well as on the yield and quality parameters of this fresh commodity. Monographic method has been used for this review. Edamame has a high nutritional and medical value. Composition of biologically active compounds in edamame plants is dependent on climate conditions, seasonal changes, cultivar properties and maturity stage. Vegetable soybean has a long cultivation history in the world. In Europe edamame is grown, but in Latvia edamame has not been grown until now, but there is some experience in soya grain growing. Pod colour is the main external quality factor of edamame. It can be influenced by light, moisture, fertilization, variety properties, planting density and harvesting procedures. Vegetation period for edamame in most cases is reported to be between 75 and 100 days.

Cattle fattening, first of all, should be beneficial for a farmer, therefore, in Latvia more suitable are such average intense fattening methods as fattening with grass fodder. In the world, demand for beef, produced in an environmentally friendly manner, ensuring the appropriate animal welfare conditions, is growing. The aim of the study was to find out the fattening results of Hereford (HE) and Aberdinanguss (AB) pure-bred bulls and their crosses when fed with grass fodder. The study was conducted in 2015 and 2016 within the framework of the project ‘Baltic Grassland Beef’. AB crossbreed bulls were slaughtered when they were 519 ± 16.6 days old, but HE pure-bred bulls − 584 ± 7.3 days old (p ≤ 0.05). The biggest live weight and slaughter weight showed HE crossbreed group bulls – 557 ± 19.4 kg and 293 ± 12.2 kg, respectively, but the biggest live weight daily gain from birth to slaughter was observed to AB pure–bred group bulls – 952 ± 24.7. Carcass conformation score in muscle development was the highest for AB pure–bred group bulls. Their average conformation score was 3.2 ± 0.08 points. In terms of fat score development, carcass with the best quality was obtained from HE crossbreed group bulls, the resulting fat score – 2.4 ± 0.10. The obtained results prove that pure-bred and crossbreed bulls used in this research are suitable for fattening with grass in the conditions of Latvia

The aim of our study was to determine up to what extent the number of services per calving (NSPC) affects dairy cow productive and reproductive performance. The study contains data from the Agricultural Data Centre of Latvia about 26888 Latvian Brown (LB) and Holstein Black and White (HBW) breed cows that were born from year 2005 – 2010, closed at least 5 full lactations and were culled from herds. In data set we have included data about cow milk productivity (calculated in ECM), longevity traits and reproduction traits. The reproduction traits included the number of services per conception (NSPC), calving interval (CI) and calving to conception interval (CCI) in the first five lactations. The average lifespan of LB and HBW dairy cows was 3149.5 days and in this period 39570.6 kg ECM were obtained. LB breed cows were characterized by 23.5 days longer lifespan and by 6035.4 kg ECM lower lifetime milk productivity than HBW breed cows, but they showed higher reproduction trait scores than LB breed group. HBW cows had a significantly (p is less than 0.05) higher NSPC, CCI, number of milking days (MD) and CI than LB breed cows in all five analysed lactations. The NSPC in the first lactation increased the calving age at the fifth lactation – cows with NSPC in the first lactation is greater than 4, fifth time calved 170.7 days in LB breed group and 190.8 days in HBW breed group later than cows with one NSPC in the first lactation.